FastQCFastQC Report
Sat 18 Jun 2016
SRR578549_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR578549_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7081581
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1338891.8906653754295826No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT278230.39289249109768004No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT78310.11058265096452333No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCACG3100.027.5671353
TATGCCG9500.023.49288448-49
CGCGTAG1750.023.06102454-55
GATCGGA22150.022.1101571
CGTACGC1950.020.69469320-21
ATCGGAA24650.019.8381482
ATCAACG52900.019.65462110-11
CTCGTAT11200.019.5018544-45
CAACGCA54000.019.2981412-13
TCTCGTA11300.018.48808142-43
ACGCAGA61450.017.11301414-15
ACACGTC14100.017.00383812-13
TCGGAAG29150.016.9385243
TCAACGC51050.016.87940210-11
ACGTACG2401.8189894E-1216.81443820-21
GTATCAA69900.016.640519
GGTATCA69550.016.5877298
CGTAGCG2451.8189894E-1216.47163656-57
AGTGGTA70100.016.4576975
CTCGCGT2600.016.4360552-53
Produced by FastQC (version 0.11.5)