##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR578549_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 7081581 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.41556200515111 31.0 31.0 34.0 30.0 34.0 2 31.784646253428438 31.0 31.0 34.0 30.0 34.0 3 31.859064522456215 31.0 31.0 34.0 30.0 34.0 4 35.56476922314381 37.0 35.0 37.0 33.0 37.0 5 35.38908952675963 37.0 35.0 37.0 33.0 37.0 6 35.378061198480964 37.0 35.0 37.0 33.0 37.0 7 35.37296544373354 37.0 35.0 37.0 32.0 37.0 8 35.33306136016802 37.0 35.0 37.0 32.0 37.0 9 36.87480521651874 39.0 37.0 39.0 32.0 39.0 10-11 36.87019021599838 39.0 37.0 39.0 32.0 39.0 12-13 36.859532426445455 39.0 37.0 39.0 32.0 39.0 14-15 38.036077748740006 40.0 37.0 41.0 33.0 41.0 16-17 37.95871105618929 40.0 37.0 41.0 33.0 41.0 18-19 37.85805330758767 40.0 37.0 41.0 32.5 41.0 20-21 37.807528431857236 40.0 37.0 41.0 32.0 41.0 22-23 37.59555803993487 39.0 36.0 41.0 32.0 41.0 24-25 37.596467935620595 39.0 36.0 41.0 32.0 41.0 26-27 37.424315064672705 39.0 36.0 41.0 32.0 41.0 28-29 37.2755012616533 39.0 36.0 41.0 31.5 41.0 30-31 37.13112199097913 39.0 36.0 41.0 31.0 41.0 32-33 36.990767527759694 39.0 36.0 40.5 31.0 41.0 34-35 36.84194525205601 39.0 35.0 40.0 30.0 41.0 36-37 36.68516656096993 39.0 35.0 40.0 30.0 41.0 38-39 36.51580106193801 38.0 35.0 40.0 30.0 41.0 40-41 36.352590403188216 38.0 35.0 40.0 30.0 41.0 42-43 36.42055749980125 38.0 35.0 40.0 30.0 41.0 44-45 36.34894686652599 38.0 35.0 40.0 30.0 41.0 46-47 36.20064827331637 38.0 35.0 40.0 30.0 41.0 48-49 36.04669423395708 38.0 35.0 40.0 29.0 41.0 50-51 35.8562908480465 38.0 34.5 40.0 28.5 41.0 52-53 35.89922610501807 38.0 34.5 40.0 28.0 41.0 54-55 35.88592596766174 38.0 34.5 40.0 28.0 41.0 56-57 35.7259233496023 38.0 34.0 40.0 28.0 41.0 58-59 35.49694941002581 38.0 34.0 40.0 28.0 41.0 60-61 35.2386884369465 37.5 34.0 40.0 28.0 41.0 62-63 34.95772151727135 37.0 34.0 40.0 27.0 41.0 64-65 34.63284844161212 37.0 33.0 40.0 26.5 41.0 66-67 34.293003921017075 36.0 33.0 39.0 26.0 41.0 68-69 33.92289214795397 36.0 33.0 39.0 26.0 40.5 70-71 33.523087358599724 35.0 32.0 39.0 26.0 40.0 72-73 33.129342939097924 35.0 32.0 38.0 26.0 40.0 74-75 32.720562329231285 35.0 31.5 37.0 25.0 39.5 76-77 31.342188841728987 34.0 29.5 36.0 23.0 39.0 78-79 31.99869337087297 34.5 31.0 36.0 24.0 39.0 80-81 31.87740251788407 35.0 31.0 36.0 24.0 38.5 82-83 31.510822512656425 34.0 31.0 35.5 23.0 37.0 84-85 31.231800215234422 34.0 31.0 35.0 23.0 37.0 86-87 30.915828894705857 34.0 31.0 35.0 21.5 36.5 88-89 30.604088409071366 34.0 31.0 35.0 20.0 36.0 90-91 30.259186260808143 34.0 30.0 35.0 19.5 36.0 92-93 29.886674882346185 34.0 30.0 35.0 17.5 35.0 94-95 29.499845514723336 34.0 29.5 35.0 9.5 35.0 96-97 29.028875896498256 34.0 29.0 35.0 2.0 35.0 98-99 28.21007604092928 34.0 29.0 35.0 2.0 35.0 100-101 27.172734661934953 33.0 27.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.3799365761665072 1101 2 0.29374127067007905 1101 3 0.3762389749845063 1101 4 0.27101380605289194 1101 5 0.27969216688114784 1101 6 0.257007287840608 1101 7 0.26645671279883487 1101 8 0.2345185828454106 1101 9 0.3287517686724257 1101 10-11 0.3193788234672681 1101 12-13 0.3288695047469332 1101 14-15 0.27055874062876484 1101 16-17 0.3038384564058916 1101 18-19 0.40079203372197014 1101 20-21 0.3179111627211526 1101 22-23 0.45240128954839065 1101 24-25 0.4399545479200597 1101 26-27 0.41082661776491847 1101 28-29 0.4088637483066222 1101 30-31 0.40323891092918984 1101 32-33 0.4351563439063071 1101 34-35 0.44145042256024425 1101 36-37 0.4784867159555404 1101 38-39 0.4068216857678735 1101 40-41 0.4187435222345499 1101 42-43 0.33593242162228876 1101 44-45 0.3353136136898769 1101 46-47 0.3450741272751685 1101 48-49 0.3638128348244365 1101 50-51 0.39460711854930963 1101 52-53 0.3553654993449058 1101 54-55 0.3871396926068442 1101 56-57 0.44395222588851624 1101 58-59 0.42269636334921046 1101 60-61 0.3311263992005138 1101 62-63 0.23013353828739014 1101 64-65 0.30819654638196425 1101 66-67 0.35520512864246 1101 68-69 0.29864206253839853 1101 70-71 0.3188043790365498 1101 72-73 0.3703529284859073 1101 74-75 0.41715586908640745 1101 76-77 0.32070585552326136 1101 78-79 0.39723287150184916 1101 80-81 0.3578742114284772 1101 82-83 0.49531166051729514 1101 84-85 0.47215090122235637 1101 86-87 0.5090184741781911 1101 88-89 0.5246946940217612 1101 90-91 0.536831173760806 1101 92-93 0.5142827811500439 1101 94-95 0.4456198647225733 1101 96-97 0.6074403077686732 1101 98-99 0.9584754079709867 1101 100-101 1.0378084113587178 1102 1 0.2691265370252083 1102 2 0.2998602741053311 1102 3 0.27261488312187865 1102 4 0.2885860071845272 1102 5 0.28194492793081594 1102 6 0.14752194592366408 1102 7 0.2540075569717857 1102 8 0.17009137834385513 1102 9 0.2538627166583325 1102 10-11 0.2869105884071459 1102 12-13 0.35853447493089874 1102 14-15 0.339402633013961 1102 16-17 0.20302194572406762 1102 18-19 0.3298808355653833 1102 20-21 0.29709455871381607 1102 22-23 0.5057676426121915 1102 24-25 0.3325319038276646 1102 26-27 0.42961380032331675 1102 28-29 0.3879132513628818 1102 30-31 0.44138133673322244 1102 32-33 0.3890223967244566 1102 34-35 0.3835985832166031 1102 36-37 0.40156275961903987 1102 38-39 0.3402647348988026 1102 40-41 0.32555313441166334 1102 42-43 0.22087758851309047 1102 44-45 0.3398191357892131 1102 46-47 0.36092663097975475 1102 48-49 0.2947805415747027 1102 50-51 0.2941957572189082 1102 52-53 0.3152929854270141 1102 54-55 0.34912270211525964 1102 56-57 0.3800008064804885 1102 58-59 0.42805639710970667 1102 60-61 0.4882657566369062 1102 62-63 0.34977715734549975 1102 64-65 0.2604236866639056 1102 66-67 0.29679091563457405 1102 68-69 0.28296918126797976 1102 70-71 0.35706264512447916 1102 72-73 0.40778800528550363 1102 74-75 0.34987800785035716 1102 76-77 0.1329403918515233 1102 78-79 0.3471932227440533 1102 80-81 0.36641667409609013 1102 82-83 0.41179131161989346 1102 84-85 0.413770752024039 1102 86-87 0.3963102525802782 1102 88-89 0.2868776242618196 1102 90-91 0.3661343034608002 1102 92-93 0.3765924882566978 1102 94-95 0.42954113415943596 1102 96-97 0.5755590609749142 1102 98-99 0.8947169390458996 1102 100-101 1.0029113508496685 1103 1 0.18433294090188213 1103 2 0.20284486698585624 1103 3 0.22309792638106885 1103 4 0.22511366241030828 1103 5 0.2614271928822802 1103 6 0.04792096029631665 1103 7 0.22136778634787646 1103 8 0.045136029748761075 1103 9 0.08822847759787322 1103 10-11 0.1322617420653316 1103 12-13 0.2119250270809374 1103 14-15 0.22767402951484428 1103 16-17 0.19349492561685366 1103 18-19 0.16174445521590286 1103 20-21 0.32269793296956095 1103 22-23 0.3940469156311295 1103 24-25 0.27857658489955384 1103 26-27 0.12379501147282923 1103 28-29 0.24379464088395508 1103 30-31 0.19575901350705038 1103 32-33 0.1536973576246936 1103 34-35 0.18379159691972546 1103 36-37 0.20263907652130797 1103 38-39 0.27233998395699643 1103 40-41 0.2542935326064395 1103 42-43 0.24346734937772396 1103 44-45 0.18621632448157754 1103 46-47 0.17883676670795268 1103 48-49 0.12838875424172613 1103 50-51 0.1645460967534973 1103 52-53 0.1374820117171751 1103 54-55 0.2903853335934343 1103 56-57 0.21535919446041163 1103 58-59 0.10562894824683866 1103 60-61 0.12831614554649207 1103 62-63 0.23733481870549156 1103 64-65 0.1277517746449064 1103 66-67 0.03961240291697976 1103 68-69 0.2515274773378664 1103 70-71 0.21117242182835128 1103 72-73 0.08397273809311656 1103 74-75 0.07534947643625145 1103 76-77 0.09325589321242589 1103 78-79 0.2007011145785782 1103 80-81 0.14855248224625583 1103 82-83 0.3690790162841999 1103 84-85 0.22618445283334765 1103 86-87 0.13075169125379205 1103 88-89 0.2332594351673123 1103 90-91 0.21722665855140022 1103 92-93 0.12803582767891086 1103 94-95 0.3270053839165392 1103 96-97 0.2606675264800309 1103 98-99 0.747935183075402 1103 100-101 0.6834025048790089 1104 1 0.26316118021245316 1104 2 0.19496166684314886 1104 3 0.15085609105143405 1104 4 0.19613260286688927 1104 5 0.18866137932504046 1104 6 0.06983748505108878 1104 7 0.08694727334459174 1104 8 0.030368642402777368 1104 9 0.19189557816797276 1104 10-11 0.05704875271904797 1104 12-13 0.17458031451519673 1104 14-15 0.27416609437409534 1104 16-17 0.3112176354891787 1104 18-19 0.082406116107542 1104 20-21 0.14528268341928197 1104 22-23 0.30232845011024523 1104 24-25 0.2122895499900821 1104 26-27 0.273669885097668 1104 28-29 0.17891338651150335 1104 30-31 0.18918421988568213 1104 32-33 0.24082595099723392 1104 34-35 0.19081464942365756 1104 36-37 0.22882745757546985 1104 38-39 0.21845098601473012 1104 40-41 0.3088427279950139 1104 42-43 0.2247182567183259 1104 44-45 0.21978616713960974 1104 46-47 0.25704664186778103 1104 48-49 0.2519850100177692 1104 50-51 0.22988203620754177 1104 52-53 0.14375555700887332 1104 54-55 0.23723740060194842 1104 56-57 0.14194211173335702 1104 58-59 0.0797595280708947 1104 60-61 0.15262752746998842 1104 62-63 0.09697951290167595 1104 64-65 0.09194988986052977 1104 66-67 0.11644310236373911 1104 68-69 0.19651806169625985 1104 70-71 0.22301974073981512 1104 72-73 0.22407254398410004 1104 74-75 0.24688382685507548 1104 76-77 0.11088786227450953 1104 78-79 0.21415196815151205 1104 80-81 0.3294911339162958 1104 82-83 0.4033475955821011 1104 84-85 0.28959704172122613 1104 86-87 0.45885039947078354 1104 88-89 0.34485532229193794 1104 90-91 0.32649461457999607 1104 92-93 0.4501442293761144 1104 94-95 0.38051949324850654 1104 96-97 0.2838150373584405 1104 98-99 0.7219013908088563 1104 100-101 0.805271013146708 1105 1 0.2488301146215015 1105 2 0.18008158566599164 1105 3 0.12871420407758194 1105 4 0.15632811384043066 1105 5 0.17637127302090505 1105 6 0.04134724780292487 1105 7 0.16274381929495263 1105 8 0.03095561457836027 1105 9 0.19376453724969167 1105 10-11 0.0778961393334825 1105 12-13 0.12282203263269764 1105 14-15 0.1136418683550886 1105 16-17 0.14229105509891582 1105 18-19 0.1476165108286196 1105 20-21 0.09403694833272169 1105 22-23 0.18405599211467205 1105 24-25 0.19312628112893293 1105 26-27 0.18026932804088602 1105 28-29 0.22018692661526273 1105 30-31 0.14342146734396266 1105 32-33 0.18721085153653405 1105 34-35 0.13942779481962475 1105 36-37 0.09542753637568069 1105 38-39 0.14348960901413932 1105 40-41 0.11090319693335715 1105 42-43 0.23049036822497015 1105 44-45 0.17125724180082358 1105 46-47 0.20684106433915161 1105 48-49 0.21321476277655194 1105 50-51 0.14576958764177306 1105 52-53 0.15660452671241387 1105 54-55 0.2001205586893704 1105 56-57 0.1280449255600118 1105 58-59 0.14411605893781854 1105 60-61 0.017811841426777164 1105 62-63 0.09199535043472196 1105 64-65 0.12266403255127045 1105 66-67 0.1855075787067193 1105 68-69 0.18625019320672465 1105 70-71 0.33127436905704144 1105 72-73 0.282552055794703 1105 74-75 0.2972129537020862 1105 76-77 0.12224466687875335 1105 78-79 0.1863237660867867 1105 80-81 0.4312789370985364 1105 82-83 0.4735867955996014 1105 84-85 0.3733045609605483 1105 86-87 0.3644664780449993 1105 88-89 0.3909853840967088 1105 90-91 0.5787444192399427 1105 92-93 0.7239924846703971 1105 94-95 0.7450332614566655 1105 96-97 0.7807986929109845 1105 98-99 1.2841285800366222 1105 100-101 1.257900853065724 1106 1 0.18448866873715986 1106 2 0.1850026170402188 1106 3 0.11585559992441574 1106 4 0.09869930413749728 1106 5 0.11846860601417575 1106 6 -0.0028557734297152138 1106 7 0.04464680792562348 1106 8 0.047415118374487975 1106 9 0.10497509811845163 1106 10-11 -0.011750331851963836 1106 12-13 0.06550892053257229 1106 14-15 0.011869452067855946 1106 16-17 -0.018315513201578426 1106 18-19 0.07570211319731612 1106 20-21 0.13222955989117224 1106 22-23 0.2243828860462358 1106 24-25 0.09723975024017761 1106 26-27 0.03961958574846847 1106 28-29 0.19755251723521638 1106 30-31 0.17904383698665072 1106 32-33 0.12627037593779988 1106 34-35 0.050735428385529246 1106 36-37 0.040143474114401556 1106 38-39 0.07770870379116701 1106 40-41 0.21893871089373818 1106 42-43 0.18759707648847268 1106 44-45 -0.04286662829976251 1106 46-47 0.07588386490061083 1106 48-49 0.11836849099368152 1106 50-51 0.12020744293617014 1106 52-53 0.11415743658240984 1106 54-55 0.17297079620647793 1106 56-57 0.2327090297352754 1106 58-59 0.12462155844557543 1106 60-61 0.1984720236835429 1106 62-63 0.18608189421904342 1106 64-65 0.08063448226614867 1106 66-67 0.0683935385136536 1106 68-69 0.19953585849781774 1106 70-71 0.21695497075008774 1106 72-73 0.195407326605455 1106 74-75 0.26700819980766966 1106 76-77 0.11587625658569678 1106 78-79 0.2394429443611159 1106 80-81 0.33349581802366757 1106 82-83 0.49604624454595836 1106 84-85 0.5057393938939789 1106 86-87 0.533187225763001 1106 88-89 0.5156221979324869 1106 90-91 0.4590904895896273 1106 92-93 0.5011141059483464 1106 94-95 0.4534913570980379 1106 96-97 0.5862508949787362 1106 98-99 1.0203115954486428 1106 100-101 1.055856433902239 1107 1 0.20547256432453764 1107 2 0.10378169689555605 1107 3 0.012495260688105247 1107 4 0.023301775443322015 1107 5 0.006649351954706617 1107 6 0.03553369447553223 1107 7 0.03500000599985498 1107 8 0.0783054220115531 1107 9 0.14157807751489315 1107 10-11 0.02448447138237242 1107 12-13 0.0908193553863299 1107 14-15 0.06074081895891936 1107 16-17 0.1471055393584777 1107 18-19 0.05208845173929433 1107 20-21 0.10138744404279265 1107 22-23 0.18824135613275672 1107 24-25 0.20596358704238327 1107 26-27 0.18302877828349295 1107 28-29 0.1024302872552667 1107 30-31 0.09957596892599696 1107 32-33 0.18562586792958058 1107 34-35 0.18780518206028063 1107 36-37 0.19251338711103472 1107 38-39 0.24308892636404522 1107 40-41 0.14484956559455497 1107 42-43 0.16414060198791702 1107 44-45 0.21864975254447216 1107 46-47 0.09002352101538236 1107 48-49 0.13500854798794393 1107 50-51 0.17234417173664696 1107 52-53 0.10849265358444882 1107 54-55 0.18743447273413238 1107 56-57 0.21709156410612707 1107 58-59 0.14063266198491675 1107 60-61 0.20445871720234265 1107 62-63 0.27242657097571765 1107 64-65 0.29866892936500733 1107 66-67 0.22742552504925584 1107 68-69 0.25736803525270346 1107 70-71 0.2563567487668479 1107 72-73 0.20541179099398477 1107 74-75 0.3147900130817902 1107 76-77 0.27207174359097763 1107 78-79 0.31954478134041153 1107 80-81 0.2722345392202392 1107 82-83 0.31403089998055833 1107 84-85 0.40115478085747824 1107 86-87 0.4865831882020579 1107 88-89 0.37124376926851 1107 90-91 0.511466923583118 1107 92-93 0.511518207135051 1107 94-95 0.5101151915257205 1107 96-97 0.5936450921176082 1107 98-99 1.0299667028392854 1107 100-101 1.1774110159512219 1108 1 0.11849733902522175 1108 2 0.01936103186032412 1108 3 0.006804995935489444 1108 4 0.01405710203257371 1108 5 0.030118274880244655 1108 6 0.05887847215112885 1108 7 -0.0222523006274713 1108 8 0.055487260476844824 1108 9 0.010437500182270298 1108 10-11 0.03480644094389618 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2316 90-91 -1.2363278972996774 2316 92-93 -1.2403427232592144 2316 94-95 -1.3580580262558257 2316 96-97 -1.4965265101160377 2316 98-99 -1.1192274245201936 2316 100-101 -1.3730858769974112 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 126.0 3 69.0 4 215.0 5 611.0 6 1192.0 7 2021.0 8 3372.0 9 5194.0 10 7034.0 11 8913.0 12 10883.0 13 12788.0 14 14960.0 15 17053.0 16 19690.0 17 22989.0 18 26376.0 19 30190.0 20 32243.0 21 34584.0 22 38459.0 23 46284.0 24 57591.0 25 71841.0 26 91822.0 27 116211.0 28 144773.0 29 179353.0 30 218294.0 31 265652.0 32 323129.0 33 402486.0 34 523045.0 35 738214.0 36 921695.0 37 1272907.0 38 1165137.0 39 253800.0 40 385.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 18.811219946571683 31.482949781681626 27.788848960342882 21.91698131140381 2 22.711284386918685 29.294136436482194 28.144943904475568 19.849635272123557 3 21.026279301189945 28.516103960400933 28.65814851231667 21.79946822609245 4 21.682690277763463 28.99534832096289 28.063939298984725 21.258022102288926 5 22.294491468879027 28.860518110248005 27.92597601963301 20.919014401239956 6 21.996374818260794 28.68453293447673 27.860281383691994 21.45881086357048 7 22.04159362491291 29.090571209814325 27.76034283942281 21.10749232584996 8 21.30590612463516 28.879709206178678 28.157186933256856 21.657197735929305 9 21.201787513725773 28.674934703726635 28.527368514995548 21.595909267552045 10-11 21.26738077900995 29.15383611152082 28.039230873455878 21.53955223601335 12-13 21.239423795336098 29.201325240790155 27.864095602380317 21.69515536149343 14-15 21.417886768505507 29.210934676875123 27.679708810786746 21.691469743832627 16-17 21.610690328434544 29.007572252429313 27.63673381577285 21.745003603363287 18-19 21.535425601671832 29.038665874094065 27.612176406100303 21.813732118133796 20-21 21.679531850269864 28.97394868278346 27.640905868400424 21.705613598546254 22-23 21.74462850040288 29.016025040873867 27.55612407580157 21.683222382921684 24-25 21.578994860046084 28.96729557989946 27.59158583372837 21.862123726326086 26-27 21.76206165465474 28.862629974635585 27.689444465312373 21.685863905397305 28-29 21.80430475895396 28.979625585148977 27.565884048480697 21.65018560741636 30-31 21.741830742137818 28.970377625243433 27.557554774925773 21.73023685769298 32-33 21.739750844851315 29.053169907272704 27.4491403497091 21.757938898166884 34-35 21.766227615229024 29.126113029603196 27.405118663495315 21.70254069167247 36-37 21.796558282461124 29.033828250772448 27.436296975316782 21.73331649144965 38-39 21.804980466019515 29.072347040304702 27.393445687965247 21.729226805710535 40-41 21.80462127764735 29.171919295334593 27.33521385324178 21.68824557377628 42-43 21.801505725337442 29.136211742590273 27.34055505723078 21.7217274748415 44-45 21.77655566444587 29.181244475071804 27.33939933969165 21.702800520790674 46-47 21.83404494708106 29.205081534362904 27.247151044175222 21.713722474380813 48-49 21.824603116432595 29.214709091861707 27.268453340010375 21.69223445169532 50-51 21.82610091910254 29.21044202841448 27.257776331662107 21.705680720820876 52-53 21.76151467764128 29.303267068775767 27.165229696850506 21.769988556732443 54-55 21.810232052770377 29.308414379856323 27.14404026058125 21.73731330679205 56-57 21.798566494806273 29.358832838642197 27.117909523135065 21.724691143416468 58-59 21.780769581901108 29.439703723557226 27.07850722753509 21.701019467006578 60-61 21.802772572960755 29.42168563771282 27.07723741350978 21.698304375816644 62-63 21.732918660868144 29.491032571435756 27.057597231575524 21.718451536120575 64-65 21.773943921126605 29.59674228407832 27.02053907589974 21.608774718895337 66-67 21.737624063327132 29.641695995962447 27.002352392274283 21.61832754843614 68-69 21.695867020504796 29.700126069837253 26.95275682007458 21.651250089583367 70-71 21.711412248688262 29.79520843488983 26.90115179898337 21.592227517438534 72-73 21.71443060505382 29.771931904445808 26.85096367541841 21.662673815081956 74-75 21.73447036268282 29.79290710195694 26.881964821963006 21.590657713397235 76-77 21.694020342534934 29.875368347026942 26.853466460165215 21.577144850272916 78-79 21.700204569237822 29.88475337484875 26.815635831312036 21.599406224601392 80-81 21.642978751376198 29.93294772645225 26.7984285111009 21.625645011070645 82-83 21.71662426238509 29.980883828837612 26.739021546326953 21.563470362450342 84-85 21.63387935354216 30.03540666903855 26.74581383761283 21.58490013980646 86-87 21.62846629883734 30.080246932321604 26.720548723429626 21.570738045411435 88-89 21.607745856249046 30.175708286033302 26.688309240031195 21.528236617686453 90-91 21.552217957043172 30.15593929850086 26.712321271232508 21.579521473223455 92-93 21.560279111028017 30.251897801632794 26.65272937886033 21.535093708478858 94-95 21.578728860470633 30.29085563551328 26.620252431681337 21.510163072334755 96-97 21.549563462179076 30.310440545436805 26.579312031588714 21.56068396079541 98-99 21.577756851641503 30.355376618634512 26.471321042564526 21.59554548715946 100-101 21.59203244534528 30.375747865533498 26.45524746240964 21.576972226711575 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 149755.0 1 85128.0 2 15433.0 3 9348.0 4 7894.0 5 6886.0 6 6225.5 7 6065.0 8 5656.0 9 5229.5 10 5064.5 11 5421.0 12 8265.5 13 8137.0 14 5591.0 15 5641.5 16 6080.0 17 6875.5 18 8134.5 19 10014.0 20 12579.5 21 16394.5 22 21047.0 23 26301.0 24 32308.0 25 39679.0 26 48888.0 27 58901.0 28 68569.0 29 77843.5 30 88459.5 31 100658.0 32 113483.0 33 127961.5 34 142279.0 35 154813.5 36 168110.0 37 181279.0 38 192555.0 39 199101.0 40 206275.5 41 218001.5 42 227466.0 43 236260.5 44 243952.5 45 250652.0 46 256418.5 47 258985.0 48 263392.5 49 266472.0 50 264644.0 51 256613.5 52 243566.5 53 232978.0 54 219987.5 55 203923.0 56 187831.5 57 169571.0 58 151073.5 59 131837.5 60 109606.5 61 86582.5 62 67345.5 63 51830.5 64 38723.0 65 27068.5 66 18079.0 67 12613.0 68 7917.5 69 4259.0 70 2268.0 71 1042.0 72 489.0 73 251.5 74 131.0 75 86.5 76 60.5 77 37.5 78 27.0 79 22.0 80 15.0 81 9.5 82 9.0 83 9.0 84 6.5 85 4.0 86 2.5 87 1.5 88 1.5 89 0.5 90 0.0 91 0.0 92 0.5 93 0.5 94 0.0 95 0.5 96 0.5 97 0.0 98 0.0 99 0.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.15777550239134452 2 0.0 3 0.0 4 7.342993040678346E-4 5 5.366033375880329E-4 6 7.060570231421486E-5 7 2.1181710694264457E-4 8 0.0 9 1.8357482601695865E-4 10-11 2.1887767717406606E-4 12-13 0.0 14-15 0.0 16-17 3.530285115710743E-5 18-19 7.060570231421486E-6 20-21 7.060570231421486E-6 22-23 5.50724478050876E-4 24-25 0.0 26-27 9.88479832399008E-5 28-29 0.0052460036819461636 30-31 0.003692678231033437 32-33 1.3415083439700823E-4 34-35 9.108135598533717E-4 36-37 4.1657364365386767E-4 38-39 0.001059085534713223 40-41 0.002908954935345652 42-43 0.0010873278156389088 44-45 5.789667589765618E-4 46-47 0.00860683511210279 48-49 0.008472684277705783 50-51 0.007667779271323734 52-53 0.014099958752148707 54-55 0.005810849300459883 56-57 8.190261468448924E-4 58-59 0.001221478650035917 60-61 0.0 62-63 1.1296912370274378E-4 64-65 7.766627254563635E-4 66-67 9.390558407790577E-4 68-69 0.0026618349772459006 70-71 0.0019345962434094872 72-73 0.005429578507963123 74-75 0.002054625937343653 76-77 0.0016027494425326775 78-79 0.0016309917234583633 80-81 2.3299881763690905E-4 82-83 0.002005201945723702 84-85 7.766627254563634E-5 86-87 0.0017863242685496362 88-89 1.976959664798016E-4 90-91 0.0010661461049446444 92-93 0.0013838717653586112 94-95 8.613895682334213E-4 96-97 9.037529896219502E-4 98-99 0.5331860215960249 100-101 1.4827197485985121E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 7081581.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 74.36804712769276 #Duplication Level Percentage of deduplicated Percentage of total 1 92.52233628555304 68.80705465248246 2 4.434988347034232 6.5964284480602 3 0.9761393587563467 2.177807335055634 4 0.4492359991765561 1.3363521583287308 5 0.279265959682231 1.0384232025404252 6 0.1998957839675045 0.8919515449633477 7 0.15302190025582282 0.7965957922855893 8 0.1245823847820102 0.7411958930199111 9 0.09006695273659321 0.6028293047186376 >10 0.753866790431981 9.83990248723028 >50 0.01541277715342081 0.7175451493052193 >100 8.087411324830302E-4 0.11159421514039664 >500 9.467983448417007E-5 0.04527462274321022 >1k 1.4201975166791393E-4 0.3281530003491392 >5k 4.733991722263798E-5 0.2756964001431294 >10k+ 9.467983444527596E-5 5.6931957936337305 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 133889 1.8906653754295826 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 27823 0.39289249109768004 No Hit AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 7831 0.11058265096452333 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 1.4121140462842972E-5 0.0 0.0 0.0 2 0.0 1.4121140462842972E-5 0.0 0.0 0.0 3 2.8242280925685944E-5 1.4121140462842972E-5 0.0 0.0 0.0 4 2.8242280925685944E-5 2.8242280925685944E-5 0.0 1.4121140462842972E-5 0.0 5 2.8242280925685944E-5 2.8242280925685944E-5 0.0 1.4121140462842972E-5 0.0 6 5.648456185137189E-5 2.8242280925685944E-5 0.0 1.4121140462842972E-5 0.0 7 5.648456185137189E-5 2.8242280925685944E-5 0.0 1.4121140462842972E-5 0.0 8 5.648456185137189E-5 2.8242280925685944E-5 0.0 1.4121140462842972E-5 0.0 9 5.648456185137189E-5 2.8242280925685944E-5 0.0 2.8242280925685944E-5 2.8242280925685944E-5 10-11 7.060570231421486E-5 2.8242280925685944E-5 0.0 4.2363421388528916E-5 4.2363421388528916E-5 12-13 7.060570231421486E-5 4.2363421388528916E-5 0.0 4.2363421388528916E-5 4.94239916199504E-5 14-15 7.060570231421486E-5 5.648456185137189E-5 0.0 4.94239916199504E-5 6.354513208279338E-5 16-17 7.060570231421486E-5 7.060570231421486E-5 0.0 5.648456185137189E-5 1.2002969393416527E-4 18-19 7.766627254563634E-5 7.060570231421486E-5 0.0 5.648456185137189E-5 1.2709026416558676E-4 20-21 8.472684277705783E-5 7.766627254563634E-5 0.0 6.354513208279338E-5 1.2709026416558676E-4 22-23 8.472684277705783E-5 8.472684277705783E-5 0.0 7.060570231421486E-5 1.2709026416558676E-4 24-25 9.178741300847933E-5 8.472684277705783E-5 0.0 7.766627254563634E-5 1.6945368555411566E-4 26-27 1.2002969393416527E-4 8.472684277705783E-5 0.0 8.472684277705783E-5 2.047565367112231E-4 28-29 1.2709026416558676E-4 8.472684277705783E-5 0.0 8.472684277705783E-5 2.188776771740661E-4 30-31 1.4121140462842972E-4 8.472684277705783E-5 0.0 8.472684277705783E-5 2.2593824740548755E-4 32-33 1.553325450912727E-4 1.2709026416558676E-4 0.0 9.88479832399008E-5 2.541805283311735E-4 34-35 1.6945368555411566E-4 1.2709026416558676E-4 0.0 1.1296912370274378E-4 2.541805283311735E-4 36-37 2.3299881763690907E-4 1.2709026416558676E-4 0.0 1.1296912370274378E-4 2.6830166879401646E-4 38-39 2.8242280925685944E-4 1.2709026416558676E-4 0.0 1.1296912370274378E-4 2.6830166879401646E-4 40-41 3.6008908180249584E-4 1.3415083439700823E-4 0.0 1.1296912370274378E-4 2.6830166879401646E-4 42-43 4.1657364365386767E-4 1.4121140462842972E-4 0.0 1.1296912370274378E-4 2.8242280925685944E-4 44-45 4.518764948109751E-4 1.4121140462842972E-4 0.0 1.1296912370274378E-4 3.106650901825454E-4 46-47 5.436639078194544E-4 1.4121140462842972E-4 0.0 1.1296912370274378E-4 3.106650901825454E-4 48-49 6.213301803650907E-4 1.4121140462842972E-4 0.0 1.1296912370274378E-4 3.3184680087680986E-4 50-51 7.413598742992561E-4 1.4121140462842972E-4 0.0 1.1296912370274378E-4 3.3890737110823133E-4 52-53 8.260867170763139E-4 1.4827197485985121E-4 0.0 1.1296912370274378E-4 3.3890737110823133E-4 54-55 8.896318491591073E-4 1.553325450912727E-4 0.0 1.1296912370274378E-4 3.459679413396528E-4 56-57 0.0010732066751760658 1.553325450912727E-4 0.0 1.1296912370274378E-4 3.530285115710743E-4 58-59 0.0012426603607301814 1.553325450912727E-4 0.0 1.1296912370274378E-4 3.671496520339173E-4 60-61 0.001362690054664347 1.623931153226942E-4 0.0 1.1296912370274378E-4 3.671496520339173E-4 62-63 0.0015462648806813055 1.6945368555411566E-4 0.0 1.1296912370274378E-4 3.671496520339173E-4 64-65 0.0017510214173925287 1.7651425578553716E-4 0.0 1.1296912370274378E-4 3.8127079249676023E-4 66-67 0.001976959664798016 1.8357482601695865E-4 0.0 1.1296912370274378E-4 3.8127079249676023E-4 68-69 0.0022170190526663467 1.8357482601695865E-4 0.0 1.1296912370274378E-4 3.8127079249676023E-4 70-71 0.0024711995809975204 2.047565367112231E-4 0.0 1.1296912370274378E-4 3.953919329596032E-4 72-73 0.0027818646711800657 2.118171069426446E-4 0.0 1.1296912370274378E-4 4.1657364365386767E-4 74-75 0.0031278326125197186 2.118171069426446E-4 0.0 1.1296912370274378E-4 4.236342138852892E-4 76-77 0.003636193669182065 2.118171069426446E-4 0.0 1.1296912370274378E-4 4.236342138852892E-4 78-79 0.004102191304455884 2.2593824740548755E-4 0.0 1.1296912370274378E-4 4.236342138852892E-4 80-81 0.0049494597322264616 2.2593824740548755E-4 0.0 1.1296912370274378E-4 4.377553543481321E-4 82-83 0.006121514390642429 2.4005938786833054E-4 0.0 1.2002969393416527E-4 4.518764948109751E-4 84-85 0.007823111816415006 2.4005938786833054E-4 0.0 1.3415083439700823E-4 4.518764948109751E-4 86-87 0.01007543372023846 2.4711995809975206E-4 0.0 1.4827197485985121E-4 4.518764948109751E-4 88-89 0.01400817133914023 2.6830166879401646E-4 0.0 1.623931153226942E-4 4.518764948109751E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTCACG 310 0.0 27.567135 3 TATGCCG 950 0.0 23.492884 48-49 CGCGTAG 175 0.0 23.061024 54-55 GATCGGA 2215 0.0 22.110157 1 CGTACGC 195 0.0 20.694693 20-21 ATCGGAA 2465 0.0 19.838148 2 ATCAACG 5290 0.0 19.654621 10-11 CTCGTAT 1120 0.0 19.50185 44-45 CAACGCA 5400 0.0 19.29814 12-13 TCTCGTA 1130 0.0 18.488081 42-43 ACGCAGA 6145 0.0 17.113014 14-15 ACACGTC 1410 0.0 17.003838 12-13 TCGGAAG 2915 0.0 16.938524 3 TCAACGC 5105 0.0 16.879402 10-11 ACGTACG 240 1.8189894E-12 16.814438 20-21 GTATCAA 6990 0.0 16.64051 9 GGTATCA 6955 0.0 16.587729 8 CGTAGCG 245 1.8189894E-12 16.471636 56-57 AGTGGTA 7010 0.0 16.457697 5 CTCGCGT 260 0.0 16.43605 52-53 >>END_MODULE