FastQCFastQC Report
Sat 18 Jun 2016
SRR578548_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR578548_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7065766
Sequences flagged as poor quality0
Sequence length101
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT106247615.036954238224137No Hit
AAGCAGTGGTATCAACGCAGAGTATTTTTTTTTTTTTTTTTTTTTTTTTT230730.3265463362358731No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGCCAGCAGCTGACCCAGC191600.2711666364269635No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTG106520.15075506321607593No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTA85810.12144472375677315No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTC82260.11642049849938423No Hit
AAGCAGTGGTATCAACGCAGAGTACCTTTTTTTTTTTTTTTTTTTTTTTT81830.11581193036961598No Hit
TAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTT80830.11439665564922472No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTGGTA2362200.091.7863165
GTATCAA2371550.091.607899
GTGGTAT2370800.091.592296
GGTATCA2371100.091.5852368
TGGTATC2371300.091.513447
AAGCAGT2381700.091.441881
AGCAGTG2391450.090.992782
GCAGTGG2387300.090.9188463
CAGTGGT2393450.090.582664
TCAACGC2364400.046.48090412-13
AACGCAG2372850.046.34112514-15
CAACGCA2364650.046.2431112-13
ATCAACG2363900.046.2423710-11
CGCAGAG2379700.046.21814716-17
TATCAAC2379800.046.15883310-11
ACGCAGA2373400.046.1193914-15
CAGAGTA2363300.045.95815718-19
AGAGTAC2321900.045.7494318-19
GAGTACT1343300.045.69684220-21
GCAGAGT2396350.045.57022516-17
Produced by FastQC (version 0.11.5)