##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR578548_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 7065766 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.15783738097186 31.0 31.0 34.0 28.0 34.0 2 31.505724220134095 33.0 31.0 34.0 28.0 34.0 3 31.632415367279357 34.0 31.0 34.0 28.0 34.0 4 35.015866927945254 37.0 35.0 37.0 32.0 37.0 5 35.03493492425308 37.0 35.0 37.0 32.0 37.0 6 35.09010672020557 37.0 35.0 37.0 32.0 37.0 7 35.00681780291054 37.0 35.0 37.0 32.0 37.0 8 35.05570309008252 37.0 35.0 37.0 32.0 37.0 9 36.576481728944884 39.0 37.0 39.0 32.0 39.0 10-11 36.456961708044105 39.0 37.0 39.0 32.0 39.0 12-13 36.5399187434172 39.0 37.0 39.0 32.0 39.0 14-15 37.84486671367266 40.0 38.0 41.0 32.0 41.0 16-17 37.811370133117904 40.0 38.0 41.0 32.0 41.0 18-19 37.728455839041374 40.0 38.0 41.0 32.0 41.0 20-21 37.63835937957753 40.0 38.0 41.0 32.0 41.0 22-23 37.37442465544429 39.5 37.5 41.0 32.0 41.0 24-25 37.33726690071536 40.0 37.0 41.0 32.0 41.0 26-27 37.262163932403084 40.0 37.0 41.0 31.0 41.0 28-29 37.13284447857458 40.0 37.0 41.0 30.5 41.0 30-31 36.901165068302575 40.0 37.0 41.0 30.0 41.0 32-33 36.61224133377755 40.0 36.0 41.0 30.0 41.0 34-35 36.44923204363122 40.0 36.0 41.0 30.0 41.0 36-37 36.14113975752947 39.0 36.0 40.5 29.0 41.0 38-39 35.119940711311415 38.0 35.0 40.0 27.5 41.0 40-41 34.88580990369621 37.0 35.0 40.0 26.5 41.0 42-43 34.5831627455537 37.0 34.0 40.0 25.0 41.0 44-45 34.305679808813366 37.0 34.0 40.0 24.0 41.0 46-47 34.143183768044395 36.5 33.5 40.0 24.0 41.0 48-49 34.08542414226568 37.0 33.5 40.0 23.5 41.0 50-51 33.08627252869682 36.0 32.5 39.5 20.0 40.5 52-53 32.50606105551755 37.0 33.0 39.5 8.5 40.5 54-55 31.89205650172961 37.0 31.5 40.0 2.0 41.0 56-57 31.226205622999686 37.0 30.5 40.0 2.0 41.0 58-59 30.876970593138807 37.0 30.0 40.0 2.0 41.0 60-61 30.604406160634248 36.0 29.5 40.0 2.0 41.0 62-63 30.27375871207736 36.0 29.0 40.0 2.0 41.0 64-65 29.98149768899791 35.0 29.0 39.0 2.0 41.0 66-67 29.649411967506424 35.0 28.5 39.0 2.0 41.0 68-69 29.319079346811087 35.0 28.0 38.5 2.0 41.0 70-71 29.03259483826665 35.0 28.0 37.5 2.0 40.0 72-73 28.716696958263267 35.0 27.5 37.0 2.0 39.5 74-75 28.357497262151053 35.0 26.5 37.0 2.0 39.0 76-77 27.99473631025992 35.0 26.0 36.0 2.0 39.0 78-79 27.659733566042238 35.0 26.0 36.0 2.0 38.5 80-81 27.346997834346624 34.0 25.5 35.0 2.0 37.0 82-83 27.087799044010232 34.0 25.0 35.0 2.0 37.0 84-85 26.828362770575758 34.0 24.5 35.0 2.0 36.5 86-87 26.597669877547602 34.0 24.0 35.0 2.0 36.0 88-89 26.35454797116123 34.0 23.0 35.0 2.0 36.0 90-91 26.138159330495803 34.0 21.5 35.0 2.0 35.5 92-93 25.86602924580293 34.0 19.5 35.0 2.0 35.0 94-95 25.596443612766116 34.0 17.5 35.0 2.0 35.0 96-97 25.334581487697157 34.0 10.0 35.0 2.0 35.0 98-99 25.04435492202827 34.0 2.0 35.0 2.0 35.0 100-101 24.225016862998295 33.0 2.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.5719427365527139 1101 2 0.6003853327009061 1101 3 0.4854835215018909 1101 4 0.5683683048255403 1101 5 0.48960975773404414 1101 6 0.4836866052888027 1101 7 0.42762121142686027 1101 8 0.4565578565948982 1101 9 0.589744627876506 1101 10-11 0.635584128203881 1101 12-13 0.657335781787225 1101 14-15 0.7970730067246947 1101 16-17 0.7157809362632719 1101 18-19 0.7562372233055044 1101 20-21 0.8116386760207703 1101 22-23 0.8611767260900933 1101 24-25 0.7960030525435613 1101 26-27 0.8443698633316004 1101 28-29 0.9151841731597301 1101 30-31 1.0044503919762775 1101 32-33 0.9820870143271136 1101 34-35 1.0655579685345842 1101 36-37 1.075978474028922 1101 38-39 1.0515943859308408 1101 40-41 1.0286741453259154 1101 42-43 1.0786206323510967 1101 44-45 1.2190975105494104 1101 46-47 1.1472957560508732 1101 48-49 1.0487148663326238 1101 50-51 1.1232905653824972 1101 52-53 0.9670979623446172 1101 54-55 0.9386281399814003 1101 56-57 0.8971667120184215 1101 58-59 0.8675599093197519 1101 60-61 0.7905504277180277 1101 62-63 0.8118031105559922 1101 64-65 0.8481080869692654 1101 66-67 0.8598086226930661 1101 68-69 0.785586933211647 1101 70-71 0.8934074242797543 1101 72-73 0.8267141231922892 1101 74-75 0.8606001261193938 1101 76-77 0.8519101714603359 1101 78-79 0.8488533704253847 1101 80-81 0.8329306741015934 1101 82-83 0.8438827005485869 1101 84-85 0.7983693044083608 1101 86-87 0.8738422257915097 1101 88-89 0.7719795641253455 1101 90-91 0.8342869810945608 1101 92-93 0.9715529671031398 1101 94-95 1.00377433524228 1101 96-97 0.9797422455733127 1101 98-99 0.9638397535659458 1101 100-101 1.1114122958790311 1102 1 0.4698012850055626 1102 2 0.4562546697865031 1102 3 0.531797128277006 1102 4 0.48867271154279024 1102 5 0.5452698237493223 1102 6 0.5771898179447987 1102 7 0.5694958770765624 1102 8 0.49602738830721904 1102 9 0.6269644324607526 1102 10-11 0.6069413234749774 1102 12-13 0.5829060196789442 1102 14-15 0.7071047519214915 1102 16-17 0.7254061167532626 1102 18-19 0.8294685213868576 1102 20-21 0.8475486637908531 1102 22-23 0.8769368302416964 1102 24-25 0.9186950189310039 1102 26-27 0.9558121841237792 1102 28-29 1.0208839956285942 1102 30-31 0.9976265317919086 1102 32-33 1.1043762769811352 1102 34-35 1.0760087187680085 1102 36-37 1.1211029904209582 1102 38-39 1.1021737265670595 1102 40-41 1.1839607331775213 1102 42-43 1.124050760042003 1102 44-45 1.2145362596781908 1102 46-47 1.2486994408081742 1102 48-49 1.2212008703040667 1102 50-51 1.3308492361345756 1102 52-53 1.1540412496952044 1102 54-55 1.114599366787285 1102 56-57 1.0067475807374358 1102 58-59 1.0922351167681654 1102 60-61 1.1098104674323999 1102 62-63 1.1091628359745052 1102 64-65 1.0881319103323612 1102 66-67 1.0933786817386064 1102 68-69 1.0378100701210293 1102 70-71 1.0836664934906004 1102 72-73 1.0844346918179397 1102 74-75 0.986687234728997 1102 76-77 1.0277608980229367 1102 78-79 1.0594834507746569 1102 80-81 1.0740707395908586 1102 82-83 1.1225343529031768 1102 84-85 1.1146821379367573 1102 86-87 1.1225951734658501 1102 88-89 1.1116867613690111 1102 90-91 1.0938846560942324 1102 92-93 1.1263835603201322 1102 94-95 1.1934495611013354 1102 96-97 1.280837062979252 1102 98-99 1.3227429425511232 1102 100-101 1.3882035955384318 1103 1 0.41776766819511835 1103 2 0.42649022580264884 1103 3 0.3513632954661077 1103 4 0.4367455866671577 1103 5 0.4184615793011872 1103 6 0.4789488015857728 1103 7 0.35630969349764285 1103 8 0.3915853610869675 1103 9 0.4865248992236104 1103 10-11 0.5221709722964789 1103 12-13 0.5360689710270847 1103 14-15 0.6179593898918014 1103 16-17 0.7143294948945567 1103 18-19 0.6559107526406791 1103 20-21 0.6866077497428122 1103 22-23 0.762119779656409 1103 24-25 0.6962913627438212 1103 26-27 0.7518146902892298 1103 28-29 0.7687736942659456 1103 30-31 0.8361876488185516 1103 32-33 0.8906291564588216 1103 34-35 0.923012196289072 1103 36-37 0.9900053274962772 1103 38-39 0.8710722276537126 1103 40-41 0.8713554817494469 1103 42-43 0.7963921070522773 1103 44-45 0.7933319471526161 1103 46-47 1.0147468068047587 1103 48-49 1.0644530606504787 1103 50-51 1.002984141070634 1103 52-53 0.8761668355510537 1103 54-55 0.7855308248073207 1103 56-57 0.7533353274190624 1103 58-59 0.6993858409361167 1103 60-61 0.7371189649957017 1103 62-63 0.6022447448884343 1103 64-65 0.5213325134164926 1103 66-67 0.5175174323594831 1103 68-69 0.7102680092252918 1103 70-71 0.6446358506228229 1103 72-73 0.708154877339318 1103 74-75 0.6956713221819975 1103 76-77 0.6954917865224424 1103 78-79 0.7057774713602569 1103 80-81 0.7232629050330814 1103 82-83 0.7694822474353877 1103 84-85 0.8052375526310627 1103 86-87 0.8108496683060267 1103 88-89 0.8320036728725135 1103 90-91 0.7868221234085659 1103 92-93 0.8525629564263291 1103 94-95 0.811203240739534 1103 96-97 0.8091923223942281 1103 98-99 0.8806633102441559 1103 100-101 0.9540321651532686 1104 1 0.29737886173059636 1104 2 0.302041773002955 1104 3 0.3139757100635734 1104 4 0.23700275822998407 1104 5 0.36076613778045186 1104 6 0.3574173728430665 1104 7 0.32132778441951615 1104 8 0.24997731531978928 1104 9 0.42541441672536706 1104 10-11 0.42433538011090377 1104 12-13 0.46704595473372024 1104 14-15 0.5704112716187311 1104 16-17 0.5310523199634858 1104 18-19 0.5240713946622861 1104 20-21 0.5924601065152544 1104 22-23 0.5749247447052426 1104 24-25 0.5811537095864239 1104 26-27 0.6142544648110686 1104 28-29 0.6276136445767619 1104 30-31 0.7785953696047727 1104 32-33 0.7336505429998184 1104 34-35 0.845609567873062 1104 36-37 0.8335836996029471 1104 38-39 0.8267633401750132 1104 40-41 0.7730672428503098 1104 42-43 0.9568488909631654 1104 44-45 0.9023472329888662 1104 46-47 1.0249382580700725 1104 48-49 0.9698973178396599 1104 50-51 0.9678090715555001 1104 52-53 0.8340260632664069 1104 54-55 0.7696579985729635 1104 56-57 0.6109775093158909 1104 58-59 0.615334394262252 1104 60-61 0.6436301113118255 1104 62-63 0.5425029631219864 1104 64-65 0.4401391019562517 1104 66-67 0.5771010420210239 1104 68-69 0.6403865896263063 1104 70-71 0.5876876617126108 1104 72-73 0.5520624700083587 1104 74-75 0.5988739174810433 1104 76-77 0.607859936206939 1104 78-79 0.5278034146555868 1104 80-81 0.6439003796181915 1104 82-83 0.6834750544927637 1104 84-85 0.6816715389950971 1104 86-87 0.6903753830587256 1104 88-89 0.70372775709723 1104 90-91 0.6933497110595646 1104 92-93 0.6125184624173023 1104 94-95 0.5164407420832191 1104 96-97 0.6238878642192276 1104 98-99 0.839078614606187 1104 100-101 0.7544772001751276 1105 1 0.2523829372252955 1105 2 0.22060452890797677 1105 3 0.2153872844191156 1105 4 0.21314632149925217 1105 5 0.2015365992235303 1105 6 0.14822632388164436 1105 7 0.1603371298592151 1105 8 0.19378648735366966 1105 9 0.14516161889034151 1105 10-11 0.2018684780700326 1105 12-13 0.16890234615742372 1105 14-15 0.06824382956803987 1105 16-17 0.06095054134991784 1105 18-19 -0.04469426746636884 1105 20-21 0.055166754868686496 1105 22-23 -0.027123442362160688 1105 24-25 0.0012262144231200978 1105 26-27 -0.3110734797413386 1105 28-29 -0.09033027269359195 1105 30-31 -0.21394684663175667 1105 32-33 -0.1920380642328965 1105 34-35 -0.110546589477309 1105 36-37 -0.08181679093117822 1105 38-39 -0.009280563686623111 1105 40-41 -0.030053482682490085 1105 42-43 0.09082596279317556 1105 44-45 0.08383684813386338 1105 46-47 0.11642423183356243 1105 48-49 0.09736389390366895 1105 50-51 0.0697107281992615 1105 52-53 0.01565483979100435 1105 54-55 -0.054958974339488265 1105 56-57 0.14215881620154747 1105 58-59 0.2058307781046267 1105 60-61 0.07968138801842528 1105 62-63 0.18008182982876875 1105 64-65 0.09522639681116374 1105 66-67 0.023705174628432246 1105 68-69 0.0764992584041444 1105 70-71 0.12197265695826687 1105 72-73 0.10594975666467477 1105 74-75 0.1192775247264457 1105 76-77 0.14532789998879636 1105 78-79 0.14491362365507143 1105 80-81 0.22883451542569233 1105 82-83 0.22654708361514864 1105 84-85 0.18899084792111154 1105 86-87 0.18817171106265462 1105 88-89 0.2882979376728265 1105 90-91 0.26596888197112634 1105 92-93 0.3001916866209342 1105 94-95 0.35107598974975573 1105 96-97 0.36625658484229007 1105 98-99 0.41040428548305385 1105 100-101 0.40374958998442523 1106 1 -0.09908423999699068 1106 2 -0.06004199926187681 1106 3 -0.08755314179648366 1106 4 -0.07902205720797895 1106 5 -0.12035140699875768 1106 6 -0.07585392225571042 1106 7 -0.13606136984774508 1106 8 -0.15232995134265792 1106 9 -0.13063259316487574 1106 10-11 -0.3069808763839674 1106 12-13 -0.2640004970610548 1106 14-15 -0.2603410030549753 1106 16-17 -0.2624573252324751 1106 18-19 -0.29328749416432487 1106 20-21 -0.10539463756739309 1106 22-23 -0.0924251136952634 1106 24-25 -0.21886644581294235 1106 26-27 -0.16853913930496134 1106 28-29 -0.21013394013109377 1106 30-31 -0.24982974786598788 1106 32-33 -0.15438336675469344 1106 34-35 -0.1544169945884022 1106 36-37 -0.2714915377020404 1106 38-39 -0.266419026847764 1106 40-41 -0.28844048119997723 1106 42-43 -0.3709107938309941 1106 44-45 -0.37352853681577614 1106 46-47 -0.40143066182664455 1106 48-49 -0.6001314024107742 1106 50-51 -0.7692906395375303 1106 52-53 -0.8230996798496477 1106 54-55 -0.8396507779832199 1106 56-57 -0.7382418155725539 1106 58-59 -0.703400319259643 1106 60-61 -0.8783301976231606 1106 62-63 -0.7344649080741554 1106 64-65 -0.6697399634854548 1106 66-67 -0.8163993520331161 1106 68-69 -0.9124964378698088 1106 70-71 -0.839311234906976 1106 72-73 -0.864953986033349 1106 74-75 -0.932114858142544 1106 76-77 -0.9390291504672597 1106 78-79 -0.85224663538202 1106 80-81 -0.8606276844939451 1106 82-83 -0.9461455187854213 1106 84-85 -0.9747745078034455 1106 86-87 -0.9179499202762962 1106 88-89 -1.0019125854269042 1106 90-91 -0.9899264758203152 1106 92-93 -0.936775880591906 1106 94-95 -0.9646042052411268 1106 96-97 -0.9081576487319474 1106 98-99 -0.9430226132993376 1106 100-101 -1.0688182208637151 1107 1 -0.3705904176642356 1107 2 -0.4066472582025398 1107 3 -0.3887689468046709 1107 4 -0.2505817420231793 1107 5 -0.31970116648392377 1107 6 -0.33724733463194667 1107 7 -0.2847013425620517 1107 8 -0.2866510028928104 1107 9 -0.2888949124416129 1107 10-11 -0.3802160682220759 1107 12-13 -0.39221361708163727 1107 14-15 -0.6243496816015082 1107 16-17 -0.4765442773781672 1107 18-19 -0.4645889442435731 1107 20-21 -0.34077432421629084 1107 22-23 -0.44129594248062176 1107 24-25 -0.4091991736172105 1107 26-27 -0.3212300779663906 1107 28-29 -0.37824199664711955 1107 30-31 -0.39883083354103377 1107 32-33 -0.32115949572560964 1107 34-35 -0.3901259843683391 1107 36-37 -0.5490284505502885 1107 38-39 -0.5137161273730655 1107 40-41 -0.6851739067834046 1107 42-43 -0.6351738970732796 1107 44-45 -0.5520113613560369 1107 46-47 -0.5852742359340439 1107 48-49 -0.6239824999791992 1107 50-51 -0.7849491194328735 1107 52-53 -0.7926800943931269 1107 54-55 -0.7619912790944916 1107 56-57 -0.8077868569507949 1107 58-59 -0.8156238753793446 1107 60-61 -0.7081600788125719 1107 62-63 -0.7255331322897014 1107 64-65 -0.7257991006400957 1107 66-67 -0.8122062334305724 1107 68-69 -0.772257980597999 1107 70-71 -0.6761518320109552 1107 72-73 -0.7146939212406132 1107 74-75 -0.752855028932931 1107 76-77 -0.6915409331534157 1107 78-79 -0.6980349806126256 1107 80-81 -0.6716589875716359 1107 82-83 -0.7244291019417339 1107 84-85 -0.8117400754952335 1107 86-87 -0.8136030808700419 1107 88-89 -0.9245283843523531 1107 90-91 -0.8294022595046506 1107 92-93 -0.7733668617579319 1107 94-95 -0.7555426985000828 1107 96-97 -0.8407566176397197 1107 98-99 -0.8232234391459734 1107 100-101 -0.882663714190624 1108 1 -0.676242967332989 1108 2 -0.6640672107516359 1108 3 -0.5623130204946527 1108 4 -0.5103834924262571 1108 5 -0.7265206271963436 1108 6 -0.6057628077606125 1108 7 -0.5200624176567672 1108 8 -0.6021154257783792 1108 9 -0.6854461265142504 1108 10-11 -0.9190580503383003 1108 12-13 -0.7665907765893394 1108 14-15 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-0.36606412661905097 2305 66-67 -0.24182356503308 2305 68-69 -0.24102792211441582 2305 70-71 -0.19304138641626878 2305 72-73 -0.1637222689161888 2305 74-75 -0.24195534111987982 2305 76-77 -0.23004898529052298 2305 78-79 -0.24711849912487338 2305 80-81 -0.20911001729626477 2305 82-83 -0.29303367922197765 2305 84-85 -0.28398834332039513 2305 86-87 -0.21282249844419354 2305 88-89 -0.15888119062297434 2305 90-91 -0.13478416360309708 2305 92-93 -0.19363592402661212 2305 94-95 -0.12948675168071233 2305 96-97 -0.1408228879490423 2305 98-99 -0.1725846802541824 2305 100-101 -0.19940247165801495 2306 1 -0.2480126142779966 2306 2 -0.28291790918564175 2306 3 -0.27075978479145846 2306 4 -0.3925393535272548 2306 5 -0.3908154389512788 2306 6 -0.2927462969188497 2306 7 -0.38205370078539147 2306 8 -0.2886324509388345 2306 9 -0.49148478960883324 2306 10-11 -0.6113088295590003 2306 12-13 -0.587579766107325 2306 14-15 -0.6960019928385535 2306 16-17 -0.5740642940812961 2306 18-19 -0.5100212005331599 2306 20-21 -0.5130331439662967 2306 22-23 -0.47545634033742346 2306 24-25 -0.5742009737549054 2306 26-27 -0.6284671525255163 2306 28-29 -0.5202598016598898 2306 30-31 -0.5093402311857105 2306 32-33 -0.6083911287072326 2306 34-35 -0.51783654465833 2306 36-37 -0.5513442809049565 2306 38-39 -0.547353351387251 2306 40-41 -0.6318829443719025 2306 42-43 -0.6817670872638573 2306 44-45 -0.7751995550395421 2306 46-47 -0.713328694074626 2306 48-49 -0.8170625842995065 2306 50-51 -0.7907712092402903 2306 52-53 -0.8545080450743896 2306 54-55 -0.7368011319189485 2306 56-57 -0.8478177336812891 2306 58-59 -0.8131376780457984 2306 60-61 -0.7366957075828395 2306 62-63 -0.8561612432504724 2306 64-65 -0.8913316134750602 2306 66-67 -0.8666721455298045 2306 68-69 -0.9208707044680757 2306 70-71 -0.8895473756573544 2306 72-73 -0.9011467418154844 2306 74-75 -0.9860521528680302 2306 76-77 -0.9578672605369185 2306 78-79 -0.9828180011599201 2306 80-81 -1.0958010593497605 2306 82-83 -1.1237115529207067 2306 84-85 -1.1118179784516293 2306 86-87 -1.140819237861745 2306 88-89 -1.185255912024921 2306 90-91 -1.1609643743016917 2306 92-93 -1.135389622329722 2306 94-95 -1.187632246363929 2306 96-97 -1.0836677019578573 2306 98-99 -1.065857753436692 2306 100-101 -1.1632408473008944 2307 1 -0.6462718163078698 2307 2 -0.6940655714526436 2307 3 -0.7095175523733204 2307 4 -0.7623625474857647 2307 5 -0.7582414796911294 2307 6 -0.5902478019197375 2307 7 -0.6023053947571739 2307 8 -0.6046546596058064 2307 9 -0.7048318332726637 2307 10-11 -0.7736154521207581 2307 12-13 -0.7334931017528561 2307 14-15 -0.9483245122532722 2307 16-17 -0.8882745102589737 2307 18-19 -0.9691506925125566 2307 20-21 -0.8503938364769112 2307 22-23 -0.8693566566168087 2307 24-25 -0.9085615836349206 2307 26-27 -0.9736319634905968 2307 28-29 -0.9106799507679426 2307 30-31 -0.9051788618656005 2307 32-33 -1.116902037083122 2307 34-35 -0.9788335392108891 2307 36-37 -1.0306477309948434 2307 38-39 -1.012681933269306 2307 40-41 -1.1083271507303678 2307 42-43 -1.3066119048333746 2307 44-45 -1.1878947254562675 2307 46-47 -1.2175972637602754 2307 48-49 -1.3242106906629942 2307 50-51 -1.2874226358765242 2307 52-53 -1.183606450418445 2307 54-55 -1.1520507466445302 2307 56-57 -1.0659994917710023 2307 58-59 -1.163842127340125 2307 60-61 -1.1336959957837927 2307 62-63 -1.1223481206974775 2307 64-65 -1.1106851241644762 2307 66-67 -1.0840889271433838 2307 68-69 -1.0113467210499074 2307 70-71 -1.0280535150524663 2307 72-73 -1.0755095884660442 2307 74-75 -0.9916906842243698 2307 76-77 -0.9366022768517048 2307 78-79 -0.9424274294264947 2307 80-81 -0.8951324006376531 2307 82-83 -1.0296487689323754 2307 84-85 -1.0192513337450677 2307 86-87 -1.093671913565398 2307 88-89 -1.101554201226751 2307 90-91 -1.0774327004458932 2307 92-93 -0.9526450949841312 2307 94-95 -0.8597690085829335 2307 96-97 -0.9419983456752234 2307 98-99 -1.056088704127074 2307 100-101 -1.1468616780764087 2308 1 -1.5478357438477275 2308 2 -1.1475687961136778 2308 3 -1.1148099841591694 2308 4 -1.0654091569921746 2308 5 -1.342499274903723 2308 6 -1.4336143276350768 2308 7 -1.3650312968622345 2308 8 -1.2811982126547363 2308 9 -1.3846830533327363 2308 10-11 -1.4344067791388895 2308 12-13 -1.2666754692398072 2308 14-15 -1.7793044515967438 2308 16-17 -1.915785016968158 2308 18-19 -1.6286525261860731 2308 20-21 -2.820941599136411 2308 22-23 -3.0567625598531123 2308 24-25 -2.2419042471050616 2308 26-27 -2.612903348758536 2308 28-29 -2.3249787930836945 2308 30-31 -2.102318425873392 2308 32-33 -2.4408651577364466 2308 34-35 -2.62652247619215 2308 36-37 -2.823991008113829 2308 38-39 -2.796418272931355 2308 40-41 -2.528351243489489 2308 42-43 -2.6278207006636833 2308 44-45 -2.693122349482877 2308 46-47 -2.800758268458047 2308 48-49 -2.48555119819704 2308 50-51 -2.484145915961939 2308 52-53 -2.3849364247284655 2308 54-55 -2.1460487746685892 2308 56-57 -2.115071046633414 2308 58-59 -1.975471169107621 2308 60-61 -1.9351475613386206 2308 62-63 -1.9561553466524622 2308 64-65 -1.974904754864884 2308 66-67 -2.2066752507183125 2308 68-69 -2.0055610235044163 2308 70-71 -2.0101336610819054 2308 72-73 -1.9354112627095432 2308 74-75 -1.7982784772335378 2308 76-77 -1.698744660689119 2308 78-79 -1.830427116256704 2308 80-81 -2.3836814428372968 2308 82-83 -2.1984864063268468 2308 84-85 -1.9313193892068519 2308 86-87 -1.8047330939723771 2308 88-89 -1.682760640173914 2308 90-91 -1.843870468020004 2308 92-93 -1.9253315239842657 2308 94-95 -1.9662724193363879 2308 96-97 -1.9239415718975934 2308 98-99 -1.9920015945993832 2308 100-101 -2.1520167873382476 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 65813.0 3 10742.0 4 8924.0 5 8427.0 6 9758.0 7 11816.0 8 14532.0 9 17222.0 10 19879.0 11 24404.0 12 24124.0 13 26906.0 14 30897.0 15 37468.0 16 48926.0 17 68529.0 18 110834.0 19 188648.0 20 278283.0 21 196052.0 22 97010.0 23 79364.0 24 81640.0 25 83203.0 26 84657.0 27 92683.0 28 105313.0 29 122910.0 30 144637.0 31 173038.0 32 213703.0 33 270864.0 34 361774.0 35 537108.0 36 915647.0 37 1183700.0 38 1097399.0 39 218641.0 40 291.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 9.723148524962596 51.44578275720371 23.181574500370004 15.649494217463694 2 15.571230025474634 53.458681243243724 16.343387807562323 14.626700923719316 3 48.590664602212776 19.26690167747974 17.326879719403493 14.815554000903989 4 15.531798626798793 17.50387608534992 18.292234107766856 48.67209118008443 5 15.323553796212789 50.854858424011084 19.292117930673328 14.529469849102803 6 48.68723757807643 18.680004013771935 17.277802128686115 15.354956279465522 7 16.190644947215972 17.99154759224131 51.540212908058805 14.277594552483915 8 48.198868828906306 18.740143014832377 18.29939079355639 14.761597362704926 9 47.9755943522839 18.407461706764785 18.743735037337125 14.873208903614197 10-11 15.000453286603884 34.8066221315822 34.990519905226655 15.20240467658726 12-13 14.784198258264524 18.751506639547273 34.93836066010027 31.525934442087927 14-15 14.949829464553446 51.53813821987646 18.219454664492837 15.292577651077249 16-17 31.43734564884997 18.519250802454376 18.356336235229897 31.687067313465754 18-19 15.207351879611034 34.85340263119291 18.320037821991654 31.619207667204403 20-21 31.68016828198016 34.94109161230297 18.362130780137647 15.016609325579225 22-23 31.576841581311744 18.483431639835675 34.84507606172686 15.094650717125727 24-25 15.048575430190192 34.74435414293705 18.917987316777136 31.28908311009563 26-27 15.300865933204937 25.11626636503931 44.11637704099483 15.466490660760929 28-29 28.563339150431055 18.445919920777456 37.75059122173276 15.240149707058734 30-31 25.65360639230417 20.098865837460643 38.08526398274076 16.162263787494428 32-33 19.380765220635364 22.28117795796145 40.197775099129565 18.14028172227362 34-35 18.497478379564754 21.47177828110879 40.787899618652105 19.242843720674358 36-37 18.55655337493973 21.571375174946265 40.91083298977418 18.96123846033983 38-39 19.12032896924522 21.672594815050335 40.398327979801245 18.8087482359032 40-41 18.809825677571443 21.43855349522357 40.52632726103413 19.22529356617085 42-43 19.3751830590252 21.83874445020232 40.452175517860226 18.333896972912253 44-45 18.7201092371515 21.89317930155251 40.259729782974944 19.126981678321044 46-47 19.04482183110726 21.726510369287674 40.04979692379981 19.178870875805252 48-49 19.141697177729895 21.89475171027427 40.02837548340683 18.935175628588997 50-51 19.307289116481257 21.892507046782598 39.22960555213791 19.57059828459823 52-53 19.693202725581585 22.886130316674624 37.780011164097345 19.64065579364644 54-55 20.5583636201811 24.015330218762596 35.727092683706665 19.699213477349634 56-57 21.032340638514412 24.98652367115037 34.06171166664635 19.919424023688865 58-59 20.927263649565408 26.244215433252776 32.65951312328027 20.169007793901546 60-61 20.839293692719536 26.5915868593052 32.19838031240607 20.370739135569195 62-63 20.81574928281161 26.35505639246623 32.740693270068775 20.088501054653392 64-65 20.637532363150235 26.02944130106506 32.87211910843661 20.460907227348095 66-67 20.654302375655075 25.442086721024655 33.76238461349889 20.141226289821386 68-69 20.143413088366387 25.003287893324867 34.365734199334206 20.487564818974548 70-71 19.99642200882595 24.698809308303453 34.864810385951884 20.43995829691871 72-73 19.892007021945243 24.7274192877638 34.385449080801465 20.995124609489487 74-75 20.46343332970349 24.97060642479258 33.93712229831509 20.628837947188842 76-77 20.692736720805463 25.491253654540593 33.268869956989064 20.547139667664876 78-79 20.616748504695238 25.524932565034536 33.1303097306407 20.72800919962954 80-81 20.649820292715752 25.89083042891234 32.79866619370179 20.660683084670115 82-83 21.522688706995343 26.081549159431923 31.95319859079733 20.4425635427754 84-85 21.029783144630986 26.889255231505764 31.259619681950753 20.821341941912497 86-87 20.954474558695395 26.569659691031156 31.358540712336406 21.11732503793704 88-89 20.938300850376812 26.441548390314658 31.372788792154328 21.24736196715421 90-91 20.814024912371224 26.601035099763493 31.10878453116576 21.476155456699527 92-93 21.11791025927583 26.728565781475027 31.15087905691182 21.002644902337327 94-95 21.061074868297275 26.61340134179404 30.75789371178537 21.56763007812331 96-97 20.895772033340204 26.430284348809447 31.378017703159244 21.295925914691104 98-99 21.38204742969737 26.509110951466695 30.73400086366715 21.374840755168787 100-101 21.5242492851769 26.595500607158563 30.63152851335359 21.24872159431095 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 9631.0 1 5657.5 2 1262.5 3 750.0 4 643.5 5 602.0 6 687.0 7 818.0 8 746.5 9 827.5 10 1405.5 11 8089.5 12 148019.5 13 164342.0 14 43175.5 15 41591.5 16 46440.0 17 50919.0 18 54058.0 19 56671.5 20 58251.0 21 58735.5 22 59538.0 23 60776.0 24 63010.0 25 66732.5 26 71303.5 27 76463.5 28 82512.5 29 89151.5 30 95008.5 31 100573.0 32 107154.0 33 115222.0 34 127951.5 35 135895.5 36 139440.0 37 146745.5 38 154273.0 39 162138.0 40 170803.5 41 179324.5 42 185485.0 43 191674.5 44 200629.0 45 207504.5 46 211445.0 47 216886.5 48 221908.5 49 222438.5 50 220628.0 51 221008.0 52 219469.5 53 222041.5 54 215765.5 55 200925.0 56 203242.5 57 191823.5 58 166500.0 59 140075.0 60 111998.0 61 91586.0 62 71471.5 63 54683.5 64 40233.0 65 28021.5 66 18621.0 67 11855.0 68 7210.0 69 4159.0 70 2661.5 71 1832.5 72 1404.5 73 1145.5 74 972.5 75 871.0 76 811.0 77 719.0 78 597.0 79 531.0 80 457.5 81 369.5 82 314.0 83 268.5 84 210.0 85 166.5 86 147.0 87 116.5 88 91.5 89 76.0 90 58.0 91 41.0 92 27.0 93 20.5 94 14.0 95 11.5 96 8.5 97 4.5 98 3.5 99 1.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0515159998222415 2 0.09322414583217163 3 0.08419469311607546 4 0.05481358992075311 5 0.08575149530850583 6 0.0013162054899638624 7 0.0012312890067403875 8 0.004882697785349812 9 0.009623868098660499 10-11 0.009899846669136793 12-13 0.017025754886306733 14-15 0.011499107103178904 16-17 0.0014081983467892937 18-19 5.024225257388937E-4 20-21 9.199285682543124E-5 22-23 3.467423064958562E-4 24-25 7.642483490112749E-4 26-27 0.0013303582371677748 28-29 0.001422351093993206 30-31 0.004705788445300905 32-33 1.8398571365086248E-4 34-35 0.002413043398267081 36-37 3.538186800978125E-4 38-39 0.0025687236175101183 40-41 0.0033683538345311743 42-43 0.001139296149914956 44-45 5.37804393748675E-4 46-47 9.694631834680061E-4 48-49 0.0022573631790240435 50-51 0.004351969765203093 52-53 0.1304458709784615 54-55 0.14008389182432593 56-57 0.12663878198060904 58-59 0.1205460243093247 60-61 0.10864356391083431 62-63 0.11317951938968826 64-65 0.11553595179913968 66-67 0.12345441385972873 68-69 0.1355267072246661 70-71 0.1236030177053698 72-73 0.11573409025999447 74-75 0.12067339903415993 76-77 0.13168423635880386 78-79 0.14527087367455985 80-81 0.1354276379942387 82-83 0.11545811168951817 84-85 0.12199668089772574 86-87 0.12283169298275658 88-89 0.11922274244575888 90-91 0.12951886603660523 92-93 0.14587236543072613 94-95 0.1550787274868712 96-97 0.15539716429895922 98-99 0.13879599182876987 100-101 0.12914381823570156 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 7065766.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.448761105670236 #Duplication Level Percentage of deduplicated Percentage of total 1 92.93798768203844 49.674203012589956 2 4.2365938011725826 4.528813799612736 3 0.9634871863128565 1.5449158934883078 4 0.45872367094778504 0.9807284760801699 5 0.2957946070329285 0.79049276438243 6 0.20660225936354848 0.6625580882768415 7 0.1515817060002023 0.5671298074396327 8 0.12199729185422852 0.5216483286284312 9 0.09300758246579123 0.44740260506069995 >10 0.5103051264341397 4.3066519087820065 >50 0.008660198323860498 0.31806029371834177 >100 0.009537879350941069 1.2221771053101822 >500 0.00221935682443204 0.7924731909897273 >1k 0.0030577805134840205 3.235899245875234 >5k 2.4659520270032425E-4 1.005465353509556 >10k+ 1.9727616216025937E-4 29.401380126255717 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 1062476 15.036954238224137 No Hit AAGCAGTGGTATCAACGCAGAGTATTTTTTTTTTTTTTTTTTTTTTTTTT 23073 0.3265463362358731 No Hit AAGCAGTGGTATCAACGCAGAGTACATGGGGAGCCAGCAGCTGACCCAGC 19160 0.2711666364269635 No Hit AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTG 10652 0.15075506321607593 No Hit AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTA 8581 0.12144472375677315 No Hit AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTC 8226 0.11642049849938423 No Hit AAGCAGTGGTATCAACGCAGAGTACCTTTTTTTTTTTTTTTTTTTTTTTT 8183 0.11581193036961598 No Hit TAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTT 8083 0.11439665564922472 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 1.4152747203912499E-5 0.0 0.0 0.0 0.0 2 2.8305494407824997E-5 0.0 0.0 0.0 0.0 3 2.8305494407824997E-5 0.0 0.0 0.0 0.0 4 2.8305494407824997E-5 1.4152747203912499E-5 0.0 0.0 0.0 5 2.8305494407824997E-5 1.4152747203912499E-5 0.0 0.0 0.0 6 2.8305494407824997E-5 1.4152747203912499E-5 0.0 0.0 0.0 7 2.8305494407824997E-5 1.4152747203912499E-5 0.0 0.0 0.0 8 2.8305494407824997E-5 1.4152747203912499E-5 0.0 1.4152747203912499E-5 0.0 9 4.245824161173749E-5 1.4152747203912499E-5 0.0 1.4152747203912499E-5 0.0 10-11 4.953461521369374E-5 2.8305494407824997E-5 0.0 1.4152747203912499E-5 0.0 12-13 7.784010962151873E-5 4.245824161173749E-5 0.0 1.4152747203912499E-5 0.0 14-15 1.273747248352125E-4 4.245824161173749E-5 0.0 1.4152747203912499E-5 0.0 16-17 1.3445109843716874E-4 4.953461521369374E-5 0.0 1.4152747203912499E-5 0.0 18-19 1.4860384564108122E-4 7.076373601956249E-5 0.0 1.4152747203912499E-5 0.0 20-21 1.7690934004890624E-4 7.076373601956249E-5 0.0 1.4152747203912499E-5 0.0 22-23 2.122912080586875E-4 7.076373601956249E-5 0.0 1.4152747203912499E-5 0.0 24-25 2.3352032886455622E-4 7.784010962151873E-5 0.0 1.4152747203912499E-5 0.0 26-27 2.759785704762938E-4 8.491648322347499E-5 0.0 2.8305494407824997E-5 7.076373601956249E-6 28-29 3.0428406488411874E-4 9.199285682543124E-5 0.0 4.953461521369374E-5 1.4152747203912499E-5 30-31 3.2551318568998746E-4 9.90692304273875E-5 0.0 5.6610988815649995E-5 1.4152747203912499E-5 32-33 3.396659328939E-4 9.90692304273875E-5 0.0 5.6610988815649995E-5 1.4152747203912499E-5 34-35 3.6797142730172496E-4 1.0614560402934375E-4 0.0 5.6610988815649995E-5 2.8305494407824997E-5 36-37 3.8920054810759374E-4 1.1322197763129999E-4 0.0 7.784010962151873E-5 2.8305494407824997E-5 38-39 4.175060425154187E-4 1.2029835123325624E-4 0.0 8.491648322347499E-5 2.8305494407824997E-5 40-41 4.5996428412715625E-4 1.273747248352125E-4 0.0 8.491648322347499E-5 2.8305494407824997E-5 42-43 5.165752729428062E-4 1.273747248352125E-4 0.0 8.491648322347499E-5 2.8305494407824997E-5 44-45 5.590335145545437E-4 1.4152747203912498E-4 0.0 8.491648322347499E-5 2.8305494407824997E-5 46-47 6.156445033701937E-4 1.4152747203912498E-4 0.0 8.491648322347499E-5 2.8305494407824997E-5 48-49 6.864082393897562E-4 1.556802192430375E-4 0.0 8.491648322347499E-5 2.8305494407824997E-5 50-51 7.996302170210562E-4 1.6275659284499373E-4 0.0 8.491648322347499E-5 2.8305494407824997E-5 52-53 9.270049418562687E-4 1.8398571365086248E-4 0.0 9.199285682543124E-5 2.8305494407824997E-5 54-55 0.001047303293089525 1.8398571365086248E-4 0.0 9.90692304273875E-5 2.8305494407824997E-5 56-57 0.001160525270720825 1.98138460854775E-4 0.0 9.90692304273875E-5 2.8305494407824997E-5 58-59 0.0012878999955560372 1.98138460854775E-4 0.0 9.90692304273875E-5 2.8305494407824997E-5 60-61 0.0013515873579736438 1.98138460854775E-4 0.0 9.90692304273875E-5 2.8305494407824997E-5 62-63 0.0015638785660323311 2.1229120805868747E-4 0.0 9.90692304273875E-5 2.8305494407824997E-5 64-65 0.0017337115324792811 2.1229120805868747E-4 0.0 9.90692304273875E-5 2.8305494407824997E-5 66-67 0.0019460027405379685 2.1229120805868747E-4 0.0 9.90692304273875E-5 3.5381868009781245E-5 68-69 0.002080453838975137 2.2644395526259998E-4 0.0 9.90692304273875E-5 4.245824161173749E-5 70-71 0.0022502868054220873 2.2644395526259998E-4 0.0 9.90692304273875E-5 4.245824161173749E-5 72-73 0.0024838071342866436 2.405967024665125E-4 0.0 9.90692304273875E-5 4.245824161173749E-5 74-75 0.002894236803200106 2.405967024665125E-4 0.0 1.1322197763129999E-4 4.245824161173749E-5 76-77 0.0033329719665213934 2.405967024665125E-4 7.076373601956249E-6 1.1322197763129999E-4 4.245824161173749E-5 78-79 0.0039769219642994125 2.54749449670425E-4 1.4152747203912499E-5 1.2029835123325624E-4 4.245824161173749E-5 80-81 0.004819010422932206 2.689021968743375E-4 1.4152747203912499E-5 1.273747248352125E-4 4.245824161173749E-5 82-83 0.005958306572847162 2.689021968743375E-4 1.4152747203912499E-5 1.273747248352125E-4 4.245824161173749E-5 84-85 0.007677865358122531 2.759785704762937E-4 1.4152747203912499E-5 1.273747248352125E-4 4.245824161173749E-5 86-87 0.010402269194875687 2.972076912821625E-4 1.4152747203912499E-5 1.273747248352125E-4 4.245824161173749E-5 88-89 0.014011219731873373 2.972076912821625E-4 1.4152747203912499E-5 1.273747248352125E-4 4.245824161173749E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGTGGTA 236220 0.0 91.786316 5 GTATCAA 237155 0.0 91.60789 9 GTGGTAT 237080 0.0 91.59229 6 GGTATCA 237110 0.0 91.585236 8 TGGTATC 237130 0.0 91.51344 7 AAGCAGT 238170 0.0 91.44188 1 AGCAGTG 239145 0.0 90.99278 2 GCAGTGG 238730 0.0 90.918846 3 CAGTGGT 239345 0.0 90.58266 4 TCAACGC 236440 0.0 46.480904 12-13 AACGCAG 237285 0.0 46.341125 14-15 CAACGCA 236465 0.0 46.24311 12-13 ATCAACG 236390 0.0 46.24237 10-11 CGCAGAG 237970 0.0 46.218147 16-17 TATCAAC 237980 0.0 46.158833 10-11 ACGCAGA 237340 0.0 46.11939 14-15 CAGAGTA 236330 0.0 45.958157 18-19 AGAGTAC 232190 0.0 45.74943 18-19 GAGTACT 134330 0.0 45.696842 20-21 GCAGAGT 239635 0.0 45.570225 16-17 >>END_MODULE