FastQCFastQC Report
Sat 18 Jun 2016
SRR578548_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR578548_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7065766
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1043401.4766976432562302No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT271480.3842187810918165No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT81400.11520336223984774No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG9650.022.59877248-49
GATCGGA22100.021.674671
CTCGTAT9900.020.35204544-45
ATCGGAA23600.019.6931842
TCTCGTA9900.018.67599342-43
CGTACGC1303.3489778E-718.2365420-21
CAACGCA56550.018.19204312-13
CGTCACG2905.3232725E-817.9876753
ATCAACG57850.017.78323210-11
AGTGGTA70550.016.6687985
GTATCAA70150.016.627249
ACACGTC13950.016.48245212-13
GGTATCA70800.016.475878
ATGCCGT14150.016.41700648-49
TCAACGC54250.016.16688310-11
GTGGTAT73800.015.9988786
ACGCAGA65050.015.81491214-15
TCGGAAG30450.015.7294823
TCGTATG14400.015.63815344-45
TGGTATC75500.015.5758327
Produced by FastQC (version 0.11.5)