##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR578548_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 7065766 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.991712717347276 31.0 31.0 34.0 30.0 34.0 2 32.260854520231774 34.0 31.0 34.0 30.0 34.0 3 32.3303449335854 34.0 31.0 34.0 30.0 34.0 4 35.90440102318701 37.0 35.0 37.0 35.0 37.0 5 35.7872563286132 37.0 35.0 37.0 33.0 37.0 6 35.75967177514794 37.0 35.0 37.0 33.0 37.0 7 35.756432635895386 37.0 35.0 37.0 33.0 37.0 8 35.674172623322086 37.0 35.0 37.0 33.0 37.0 9 37.31199717058278 39.0 37.0 39.0 34.0 39.0 10-11 37.30561201715426 39.0 37.0 39.0 33.5 39.0 12-13 37.299236912176255 39.0 37.0 39.0 33.5 39.0 14-15 38.612834687704066 40.0 38.0 41.0 34.0 41.0 16-17 38.5594249795422 40.0 38.0 41.0 34.0 41.0 18-19 38.48328150125549 40.0 38.0 41.0 33.5 41.0 20-21 38.41451259495432 40.0 38.0 41.0 34.0 41.0 22-23 38.290098129488015 40.0 38.0 41.0 33.0 41.0 24-25 38.20489024119961 40.0 37.5 41.0 33.0 41.0 26-27 38.03950003439118 40.0 37.0 41.0 33.0 41.0 28-29 37.94217824366106 40.0 37.0 41.0 33.0 41.0 30-31 37.84413841330154 40.0 37.0 41.0 33.0 41.0 32-33 37.710276493730476 40.0 37.0 41.0 32.0 41.0 34-35 37.57882690992031 40.0 37.0 41.0 32.0 41.0 36-37 37.451803456270696 40.0 36.0 41.0 31.5 41.0 38-39 37.321398203676715 40.0 36.0 41.0 31.0 41.0 40-41 37.17429469076672 39.0 36.0 41.0 31.0 41.0 42-43 37.01132290540049 39.0 35.5 41.0 31.0 41.0 44-45 36.853800847636336 39.0 35.0 41.0 30.0 41.0 46-47 36.935894282375045 39.0 35.0 41.0 31.0 41.0 48-49 36.91595553829549 39.0 35.0 41.0 31.0 41.0 50-51 36.89083752561294 39.0 35.0 41.0 31.0 41.0 52-53 36.90103521684698 39.0 35.0 41.0 31.0 41.0 54-55 36.771466745431425 39.0 35.0 41.0 31.0 41.0 56-57 36.62523206967228 39.0 35.0 41.0 30.0 41.0 58-59 36.41385109555001 39.0 35.0 41.0 30.0 41.0 60-61 36.19069892775957 38.5 35.0 40.5 30.0 41.0 62-63 35.92181399723682 38.0 35.0 40.0 29.0 41.0 64-65 35.616256043576875 37.5 34.0 40.0 29.0 41.0 66-67 35.30079406818737 37.0 34.0 40.0 29.0 41.0 68-69 34.979203670203624 36.5 34.0 39.5 28.5 41.0 70-71 34.61415804033136 36.0 34.0 39.0 28.0 41.0 72-73 34.191950313667334 35.5 33.5 39.0 28.0 40.5 74-75 33.810125611292534 35.0 33.0 37.5 27.0 40.0 76-77 32.37054786416646 34.5 31.5 36.0 25.5 39.0 78-79 32.97267451257231 35.0 32.5 37.0 26.0 39.0 80-81 32.91848392658348 35.0 33.0 36.0 26.0 39.0 82-83 32.64116877066124 35.0 33.0 36.0 26.0 37.5 84-85 32.35386793165808 35.0 33.0 35.5 26.0 37.0 86-87 32.071271678116716 35.0 32.5 35.0 26.0 37.0 88-89 31.815155780703748 35.0 32.0 35.0 25.0 36.0 90-91 31.569726551940725 35.0 32.0 35.0 25.0 36.0 92-93 31.328064430664703 35.0 32.0 35.0 24.5 36.0 94-95 31.070760339360234 35.0 32.0 35.0 23.5 35.5 96-97 30.463263643319067 34.0 31.5 35.0 17.5 35.0 98-99 30.435511875145597 34.0 31.0 35.0 18.5 35.0 100-101 29.854057012360727 34.0 30.5 35.0 9.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.41499053513745565 1101 2 0.39632410174098 1101 3 0.37287578683913836 1101 4 0.2677397079281221 1101 5 0.28151441361242036 1101 6 0.2972079042501363 1101 7 0.33239099789891213 1101 8 0.399779317566626 1101 9 0.46322632047598233 1101 10-11 0.4236955296668796 1101 12-13 0.42173770044252024 1101 14-15 0.5833627277161284 1101 16-17 0.5641225084680102 1101 18-19 0.570373277464121 1101 20-21 0.6295332387484578 1101 22-23 0.6742870388256748 1101 24-25 0.6930548533379763 1101 26-27 0.7263850193762096 1101 28-29 0.7665805235135537 1101 30-31 0.7222848987193373 1101 32-33 0.6892206874498399 1101 34-35 0.6902819232949113 1101 36-37 0.6893115555257836 1101 38-39 0.6918486985709933 1101 40-41 0.7140327968882971 1101 42-43 0.7264625931974606 1101 44-45 0.7103511497536132 1101 46-47 0.7598077432439894 1101 48-49 0.7047647204902816 1101 50-51 0.7005039141682303 1101 52-53 0.6698803167161032 1101 54-55 0.7686359662192004 1101 56-57 0.7495468177587838 1101 58-59 0.7927498220151392 1101 60-61 0.7021045479436978 1101 62-63 0.7563827362187183 1101 64-65 0.7704288529239278 1101 66-67 0.7650514033741374 1101 68-69 0.7243095377878817 1101 70-71 0.6693295618783495 1101 72-73 0.6697811665820552 1101 74-75 0.6980779821532082 1101 76-77 0.6857548983816102 1101 78-79 0.6021251492985371 1101 80-81 0.5062912439456397 1101 82-83 0.5012719760675637 1101 84-85 0.48017986257772804 1101 86-87 0.43483593906533713 1101 88-89 0.4919624811010834 1101 90-91 0.5130903073764692 1101 92-93 0.5592133635879541 1101 94-95 0.5677295647919252 1101 96-97 0.9251006939965123 1101 98-99 0.7026596058483996 1101 100-101 0.7034376187367783 1102 1 0.4272516189979072 1102 2 0.4088043668541417 1102 3 0.35350267425809534 1102 4 0.25832749641621433 1102 5 0.3389897999070115 1102 6 0.3112776279590861 1102 7 0.28877926243345087 1102 8 0.35205334147414646 1102 9 0.4798297685251143 1102 10-11 0.4393712236821088 1102 12-13 0.4521422504997261 1102 14-15 0.5394396263129764 1102 16-17 0.5781181162162596 1102 18-19 0.5878481745618132 1102 20-21 0.6154537889634639 1102 22-23 0.5990081203190343 1102 24-25 0.6583034626774307 1102 26-27 0.678105104065132 1102 28-29 0.6887461718967032 1102 30-31 0.7548142623688534 1102 32-33 0.7619743136876309 1102 34-35 0.8407947226321326 1102 36-37 0.8730411664038371 1102 38-39 0.8449121495132275 1102 40-41 0.8203300234574797 1102 42-43 0.8044072192642844 1102 44-45 0.8380900711533457 1102 46-47 0.9238759257196918 1102 48-49 0.9230161834783246 1102 50-51 0.9037173013307651 1102 52-53 0.8686683971442832 1102 54-55 0.898707988627578 1102 56-57 0.8135013224469105 1102 58-59 0.8492731785371106 1102 60-61 0.9061431787183878 1102 62-63 0.8754814356387826 1102 64-65 0.8186658872334647 1102 66-67 0.7999807354625617 1102 68-69 0.8213540678563973 1102 70-71 0.8485115720568004 1102 72-73 0.8454097451494462 1102 74-75 0.6716215982651406 1102 76-77 0.6963334657711115 1102 78-79 0.7304370622951311 1102 80-81 0.6997300509364237 1102 82-83 0.7261445402746602 1102 84-85 0.7457967372407452 1102 86-87 0.7065468868185789 1102 88-89 0.6639318984044564 1102 90-91 0.7121347164608061 1102 92-93 0.7236339624596475 1102 94-95 0.7722957076321606 1102 96-97 1.218440399163324 1102 98-99 0.9677714452024517 1102 100-101 0.9666615367831497 1103 1 0.3500054357910898 1103 2 0.31727323327401535 1103 3 0.2920641621418625 1103 4 0.2442653296567201 1103 5 0.21299056267103822 1103 6 0.29123483063225564 1103 7 0.28387636546746364 1103 8 0.11874849024441403 1103 9 0.2263895714329891 1103 10-11 0.42123589580317855 1103 12-13 0.3680968421315356 1103 14-15 0.512448147366662 1103 16-17 0.4982479479096966 1103 18-19 0.4137855636025023 1103 20-21 0.42314277495844976 1103 22-23 0.4388568781527695 1103 24-25 0.3010513898865099 1103 26-27 0.2846128728175188 1103 28-29 0.5248466311670015 1103 30-31 0.32344418623789295 1103 32-33 0.5169400957985815 1103 34-35 0.5224366484322758 1103 36-37 0.6413438098941597 1103 38-39 0.6741571801209574 1103 40-41 0.5339234247365354 1103 42-43 0.6036277034810453 1103 44-45 0.7552677040100164 1103 46-47 0.6240988450382119 1103 48-49 0.6098237554112416 1103 50-51 0.6000816387066621 1103 52-53 0.6563323361947582 1103 54-55 0.6869083445426796 1103 56-57 0.615950734323448 1103 58-59 0.3598785217885876 1103 60-61 0.41856732755881154 1103 62-63 0.24503112558357287 1103 64-65 0.4744666800638413 1103 66-67 0.44578649881435695 1103 68-69 0.4419169021794289 1103 70-71 0.42796952437932134 1103 72-73 0.5632850597098411 1103 74-75 0.5559802607397017 1103 76-77 0.3681604867777466 1103 78-79 0.48089769688844797 1103 80-81 0.5153368521329256 1103 82-83 0.5562873610835837 1103 84-85 0.5749834614423364 1103 86-87 0.451704098748678 1103 88-89 0.4004856393521692 1103 90-91 0.51327163077951 1103 92-93 0.49322860277443326 1103 94-95 0.5199515746354173 1103 96-97 0.6729004492112196 1103 98-99 0.43552378126049973 1103 100-101 0.6681555896229554 1104 1 0.3051602406540539 1104 2 0.28580087255161146 1104 3 0.2604943148067278 1104 4 0.19692203123166507 1104 5 0.22594622346100834 1104 6 0.22852196432628347 1104 7 0.23336854434062104 1104 8 0.28188410083863147 1104 9 0.3103876645613184 1104 10-11 0.30719712067212157 1104 12-13 0.3113135058809178 1104 14-15 0.3744439856487176 1104 16-17 0.4010742911374692 1104 18-19 0.42589494000690564 1104 20-21 0.35698038429787715 1104 22-23 0.4798464085773375 1104 24-25 0.45877324156376886 1104 26-27 0.5585668221736029 1104 28-29 0.5585281498260386 1104 30-31 0.5462021920873639 1104 32-33 0.5390165618617075 1104 34-35 0.5307985012874994 1104 36-37 0.5839074658346277 1104 38-39 0.6250890160028248 1104 40-41 0.5370269892704513 1104 42-43 0.6025658790527686 1104 44-45 0.6082175309538513 1104 46-47 0.4218438402171074 1104 48-49 0.5607360798318268 1104 50-51 0.4571240754482204 1104 52-53 0.4930158406196412 1104 54-55 0.5348317534526927 1104 56-57 0.5391944735580623 1104 58-59 0.5736901843181883 1104 60-61 0.4519732316785223 1104 62-63 0.3216494225432527 1104 64-65 0.4992879906272947 1104 66-67 0.5805672095817513 1104 68-69 0.6082364156484132 1104 70-71 0.5174116787742875 1104 72-73 0.6800870344555463 1104 74-75 0.6012525619662838 1104 76-77 0.5027300910073436 1104 78-79 0.4877840459793532 1104 80-81 0.655833562633859 1104 82-83 0.6863843566780687 1104 84-85 0.7111573885068836 1104 86-87 0.692094810105452 1104 88-89 0.6579052613218508 1104 90-91 0.6959358862793117 1104 92-93 0.727845037540849 1104 94-95 0.7272972934107891 1104 96-97 1.0747833698596416 1104 98-99 0.8523735479773471 1104 100-101 0.885870726004633 1105 1 0.17105590196522513 1105 2 0.13943120430084122 1105 3 0.15271378354359655 1105 4 0.07732893188386925 1105 5 0.1096197581432321 1105 6 0.06949063452437088 1105 7 0.09281849665848796 1105 8 0.15683964307764597 1105 9 0.14480825259278873 1105 10-11 0.1088975045399323 1105 12-13 0.12918912432353835 1105 14-15 0.15833120692912672 1105 16-17 0.16509019626165156 1105 18-19 0.08721296114140387 1105 20-21 0.17389624295670103 1105 22-23 0.18513252461136886 1105 24-25 0.2000183635155608 1105 26-27 0.14615097976848546 1105 28-29 0.20365537634753395 1105 30-31 0.169925787902244 1105 32-33 0.19348055722092994 1105 34-35 0.14063465375291884 1105 36-37 0.18127500812332187 1105 38-39 0.19978235900799746 1105 40-41 0.21452663766110192 1105 42-43 0.1263041402630236 1105 44-45 0.27369150050185453 1105 46-47 0.23291894991347561 1105 48-49 0.19139342108017132 1105 50-51 0.15399687408885399 1105 52-53 0.21683496936753954 1105 54-55 0.3252079786417852 1105 56-57 0.32583429409889675 1105 58-59 0.31743223866190107 1105 60-61 0.30868923777714485 1105 62-63 0.30748543574276965 1105 64-65 0.2742398041585261 1105 66-67 0.3333214194842995 1105 68-69 0.32973786839534824 1105 70-71 0.35971003856761286 1105 72-73 0.3918984461086765 1105 74-75 0.39650323161568934 1105 76-77 0.3799168453222066 1105 78-79 0.35015842076395387 1105 80-81 0.30644132204623276 1105 82-83 0.33662459749101714 1105 84-85 0.41704841468460785 1105 86-87 0.31280605379664195 1105 88-89 0.2229497510106171 1105 90-91 0.2872165791910426 1105 92-93 0.29533742530886187 1105 94-95 0.307345508338976 1105 96-97 0.6796779915322055 1105 98-99 0.4699237402181744 1105 100-101 0.4740816870732658 1106 1 -0.029539025706707633 1106 2 -0.10539457370647654 1106 3 -0.07820402314262509 1106 4 -0.06163775237828162 1106 5 -0.052775013429176454 1106 6 -0.0519097653550773 1106 7 -0.047819564606172094 1106 8 -0.04712528538121319 1106 9 -0.01608882407028034 1106 10-11 -0.08982715753476356 1106 12-13 -0.10800685743986804 1106 14-15 -0.18355966911534694 1106 16-17 -0.1603902852414123 1106 18-19 -0.1715209302318499 1106 20-21 -0.18826627110670557 1106 22-23 -0.16346188622397761 1106 24-25 -0.04851453932378291 1106 26-27 -0.08504579005966661 1106 28-29 -0.09788851025069079 1106 30-31 -0.2167151640006182 1106 32-33 -0.24756554203550962 1106 34-35 -0.32926336632029063 1106 36-37 -0.26444196434134426 1106 38-39 -0.3390261751179793 1106 40-41 -0.32506682330753023 1106 42-43 -0.24903954044250298 1106 44-45 -0.2516668857180022 1106 46-47 -0.3166748612602319 1106 48-49 -0.3248752901953438 1106 50-51 -0.2770297699322555 1106 52-53 -0.17053261288655364 1106 54-55 -0.23442572931026007 1106 56-57 -0.21421777798788355 1106 58-59 -0.1700504799698237 1106 60-61 -0.28615928935609247 1106 62-63 -0.20071414752867867 1106 64-65 -0.1686957948570651 1106 66-67 -0.1790587886186117 1106 68-69 -0.18571637716851086 1106 70-71 -0.15225178938824513 1106 72-73 -0.14027381791217408 1106 74-75 -0.16076461631131878 1106 76-77 -0.19094749543002365 1106 78-79 -0.16150922588294492 1106 80-81 -0.16923532724732127 1106 82-83 -0.1537046479727593 1106 84-85 -0.13397491633443082 1106 86-87 -0.18237113667481708 1106 88-89 -0.24584288261658216 1106 90-91 -0.17949612420650496 1106 92-93 -0.1427901626673176 1106 94-95 -0.10205959394763653 1106 96-97 0.12278928709499226 1106 98-99 -0.06748095189460912 1106 100-101 -0.09897900902028667 1107 1 -0.16788701924656735 1107 2 -0.2604425374561572 1107 3 -0.18408635713882404 1107 4 -0.14608932021497623 1107 5 -0.16128358600376203 1107 6 -0.1635292123523726 1107 7 -0.18482317637832324 1107 8 -0.1634666596524852 1107 9 -0.20072888537615086 1107 10-11 -0.23927500869826446 1107 12-13 -0.18852725784518753 1107 14-15 -0.249786707277039 1107 16-17 -0.26860433528304384 1107 18-19 -0.2760910373592651 1107 20-21 -0.25731244947614584 1107 22-23 -0.18595114231518295 1107 24-25 -0.13332379719316378 1107 26-27 -0.16645376007357982 1107 28-29 -0.18525544501313362 1107 30-31 -0.2142120318429619 1107 32-33 -0.26730851115485876 1107 34-35 -0.2873854756416776 1107 36-37 -0.2650772685149434 1107 38-39 -0.23992123471191462 1107 40-41 -0.22433619038916675 1107 42-43 -0.3535986013884056 1107 44-45 -0.25561382320959325 1107 46-47 -0.23403880970635527 1107 48-49 -0.3500666128355263 1107 50-51 -0.292791937180219 1107 52-53 -0.31756138202970874 1107 54-55 -0.2975102379057404 1107 56-57 -0.31978855810341145 1107 58-59 -0.26715023104244295 1107 60-61 -0.3528947453744351 1107 62-63 -0.3812108833245347 1107 64-65 -0.39856516624453775 1107 66-67 -0.27590095543060045 1107 68-69 -0.2708557512630634 1107 70-71 -0.26976241287978553 1107 72-73 -0.2840892826012009 1107 74-75 -0.24382968410330363 1107 76-77 -0.3677632032857545 1107 78-79 -0.19436337784549806 1107 80-81 -0.15793120054145504 1107 82-83 -0.2266722525090259 1107 84-85 -0.2451097076558355 1107 86-87 -0.20052091721634469 1107 88-89 -0.20941439256542083 1107 90-91 -0.20132494159906145 1107 92-93 -0.18689645883847206 1107 94-95 -0.16356459774490517 1107 96-97 0.09461253261114422 1107 98-99 -0.1308480920168762 1107 100-101 -0.2732884077017026 1108 1 -0.37277784233091893 1108 2 -0.39329652797965053 1108 3 -0.34686766570431615 1108 4 -0.2551858903974775 1108 5 -0.267879898881894 1108 6 -0.2858031067608806 1108 7 -0.2532851873772799 1108 8 -0.24746972238241938 1108 9 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2305 18-19 -0.17924717025989167 2305 20-21 -0.1451668383635223 2305 22-23 -0.15982593378178223 2305 24-25 -0.11422570376640095 2305 26-27 -0.16216740609210234 2305 28-29 -0.12622383272006488 2305 30-31 -0.18103767373627022 2305 32-33 -0.19881682456183114 2305 34-35 -0.1787416634797907 2305 36-37 -0.12857010961841553 2305 38-39 -0.1643534297196112 2305 40-41 -0.19990537116835583 2305 42-43 -0.2473229738128495 2305 44-45 -0.19504312023322257 2305 46-47 -0.1270074513747801 2305 48-49 -0.17465888526582773 2305 50-51 -0.1605627746434095 2305 52-53 -0.20125735782389853 2305 54-55 -0.11150216150196002 2305 56-57 -0.09958783873701549 2305 58-59 -0.09696693263827427 2305 60-61 -0.13839692192924957 2305 62-63 -0.12368177210344555 2305 64-65 -0.08078015850050946 2305 66-67 -0.09624869034657735 2305 68-69 -0.1172715282555643 2305 70-71 -0.11657273605861462 2305 72-73 -0.12366462369708842 2305 74-75 -0.14500830970970213 2305 76-77 -0.1749817464168899 2305 78-79 -0.17540474286332852 2305 80-81 -0.09831747563961812 2305 82-83 -0.04790073815544105 2305 84-85 -0.050776990304477465 2305 86-87 -0.04282097669279494 2305 88-89 -0.01943620680894398 2305 90-91 -0.06423006323555924 2305 92-93 -0.053824663294467 2305 94-95 -0.15915317517462313 2305 96-97 0.1525096271226296 2305 98-99 -0.10772145070433936 2305 100-101 -0.048885392663930105 2306 1 -0.23745260693602077 2306 2 -0.2446851539703161 2306 3 -0.25156648405376814 2306 4 -0.1889304815070716 2306 5 -0.19381558330118054 2306 6 -0.16650306878170085 2306 7 -0.19924076758114495 2306 8 -0.1858951241149427 2306 9 -0.3592707998529221 2306 10-11 -0.28824255827809964 2306 12-13 -0.3073869820366184 2306 14-15 -0.33428564842889585 2306 16-17 -0.38130177860229963 2306 18-19 -0.37080782909826837 2306 20-21 -0.4238209765193304 2306 22-23 -0.35982443035981504 2306 24-25 -0.34340370443887736 2306 26-27 -0.3868298535863346 2306 28-29 -0.4842769557697011 2306 30-31 -0.494805970772461 2306 32-33 -0.5936067593970833 2306 34-35 -0.5016624189812191 2306 36-37 -0.5583060474464787 2306 38-39 -0.705800173727674 2306 40-41 -0.5927874522367276 2306 42-43 -0.6770351711994422 2306 44-45 -0.6954366828352931 2306 46-47 -0.6011431620034742 2306 48-49 -0.5270271861489775 2306 50-51 -0.5272026377386325 2306 52-53 -0.46347219113092564 2306 54-55 -0.5611611488686492 2306 56-57 -0.5276988144003667 2306 58-59 -0.5369666762582241 2306 60-61 -0.5185176170798016 2306 62-63 -0.49530830448102137 2306 64-65 -0.5238770642323942 2306 66-67 -0.5312484294942905 2306 68-69 -0.554271600220865 2306 70-71 -0.5780664630690922 2306 72-73 -0.6067672578971894 2306 74-75 -0.6201567800482763 2306 76-77 -0.5513729310626658 2306 78-79 -0.43852863445534496 2306 80-81 -0.4608898905690637 2306 82-83 -0.4550034544272492 2306 84-85 -0.4741071029069843 2306 86-87 -0.4878571316714968 2306 88-89 -0.5018087012237302 2306 90-91 -0.49414382240381016 2306 92-93 -0.5144205355857068 2306 94-95 -0.5101137368427047 2306 96-97 -0.2776762150594543 2306 98-99 -0.5487265309665474 2306 100-101 -0.5386016729048961 2307 1 -0.48140386096202903 2307 2 -0.4637973078745219 2307 3 -0.49637321643186283 2307 4 -0.393444566588947 2307 5 -0.38495495625736 2307 6 -0.38609864391995785 2307 7 -0.43175337042794126 2307 8 -0.355598970404742 2307 9 -0.5062734017976709 2307 10-11 -0.5969338105787543 2307 12-13 -0.5656450558371802 2307 14-15 -0.916706543406498 2307 16-17 -0.8016475341166966 2307 18-19 -0.7383457498765011 2307 20-21 -0.6457321812354948 2307 22-23 -0.6161906998646955 2307 24-25 -0.6251948535064287 2307 26-27 -0.7074730069152224 2307 28-29 -0.747609416620989 2307 30-31 -0.7577479100197877 2307 32-33 -0.7898644202462677 2307 34-35 -0.7678233164755 2307 36-37 -0.8602658807514416 2307 38-39 -0.8523632374902235 2307 40-41 -0.6923203475911137 2307 42-43 -0.8462352281936418 2307 44-45 -0.6520973597816564 2307 46-47 -0.7083368719507277 2307 48-49 -0.7209424942230385 2307 50-51 -0.7966646595526825 2307 52-53 -0.9041194364160319 2307 54-55 -0.842680901027606 2307 56-57 -0.8740796464762397 2307 58-59 -0.904335433801684 2307 60-61 -0.9373689075892244 2307 62-63 -0.9511985511971872 2307 64-65 -0.9014069305729606 2307 66-67 -0.898891989824655 2307 68-69 -0.8687953687109129 2307 70-71 -0.9469421408927445 2307 72-73 -1.2341129271042988 2307 74-75 -0.9758131984923537 2307 76-77 -0.9048273394755775 2307 78-79 -0.8972602237805134 2307 80-81 -0.8399729540423877 2307 82-83 -0.822951167794681 2307 84-85 -0.8064470583628385 2307 86-87 -0.7650444781413555 2307 88-89 -0.8427429191334426 2307 90-91 -0.9336933746577181 2307 92-93 -0.8663678497675988 2307 94-95 -0.9564260113359531 2307 96-97 -0.7119927331466087 2307 98-99 -1.1208390302066533 2307 100-101 -1.1102879629456268 2308 1 -1.409893538727271 2308 2 -0.8611610617974854 2308 3 -0.8653750199273276 2308 4 -0.572284565574158 2308 5 -0.6585241526799521 2308 6 -0.6661452141397106 2308 7 -0.6115391371157486 2308 8 -0.6156723306377501 2308 9 -0.8882928012586362 2308 10-11 -0.8512929990404885 2308 12-13 -0.7787930557444369 2308 14-15 -1.0296236705916684 2308 16-17 -0.9390186887745884 2308 18-19 -0.9670283622909039 2308 20-21 -0.9531366786810835 2308 22-23 -1.054294809657236 2308 24-25 -1.1601841581472954 2308 26-27 -1.2220756224241427 2308 28-29 -1.2614464980643163 2308 30-31 -1.15371995644729 2308 32-33 -1.2293975363236953 2308 34-35 -1.3060910970825717 2308 36-37 -1.336860766761248 2308 38-39 -1.3593790315861582 2308 40-41 -1.3109025510789039 2308 42-43 -1.2499381247052384 2308 44-45 -1.2056018392738963 2308 46-47 -1.272881806959667 2308 48-49 -1.2905237983109572 2308 50-51 -1.3224387223760417 2308 52-53 -1.3192361501770478 2308 54-55 -1.2754738309383242 2308 56-57 -1.2095883495673263 2308 58-59 -1.227907121887057 2308 60-61 -1.2038178626243834 2308 62-63 -1.2210865705273548 2308 64-65 -1.2126612893163724 2308 66-67 -1.2243066657473065 2308 68-69 -1.2795979949460374 2308 70-71 -1.2301870471968215 2308 72-73 -1.2920578297526788 2308 74-75 -1.4306016223010545 2308 76-77 -1.331862550383331 2308 78-79 -1.6939156895754266 2308 80-81 -1.3860632997607638 2308 82-83 -1.2961865854465557 2308 84-85 -1.2041509320683055 2308 86-87 -1.194469226687442 2308 88-89 -1.2005845537626456 2308 90-91 -1.2566880401653506 2308 92-93 -1.170476451167513 2308 94-95 -1.3065762407799788 2308 96-97 -1.1238814668206842 2308 98-99 -1.48685837843 2308 100-101 -1.489949502135424 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 36.0 3 53.0 4 160.0 5 456.0 6 849.0 7 1661.0 8 3051.0 9 4754.0 10 6306.0 11 7856.0 12 9142.0 13 10428.0 14 11601.0 15 12925.0 16 14883.0 17 17413.0 18 19833.0 19 22542.0 20 24551.0 21 25301.0 22 27943.0 23 32612.0 24 40369.0 25 50562.0 26 64831.0 27 82479.0 28 104583.0 29 130769.0 30 162386.0 31 200250.0 32 246697.0 33 309794.0 34 411229.0 35 616462.0 36 832054.0 37 1395403.0 38 1668110.0 39 493787.0 40 1645.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 18.86745716088196 31.1437461703079 27.84356894111039 22.145227727699744 2 22.966750527960944 28.925635042989235 28.037525393014 20.07008903603582 3 21.275503510016698 27.983068357113467 28.568899124756857 22.172529008112974 4 21.804665894867345 28.53190182486194 28.091848836781423 21.57158344348929 5 22.510598392741038 28.407188310164578 27.951139689887505 21.13107360720688 6 22.199206710213726 28.185139445602925 27.904985814701476 21.710668029481873 7 22.325392604283813 28.545892971830654 27.815299855670283 21.31341456821525 8 21.55728296054317 28.397891634902955 28.195151156586025 21.849674247967855 9 21.44420664005038 28.197518024168673 28.579253759714835 21.779021576066118 10-11 21.47561920391929 28.688481899910073 28.066242216342857 21.76965667982778 12-13 21.462641134733303 28.709173216322192 27.879503793360833 21.94868185558367 14-15 21.62200814462296 28.717870079478995 27.739886942194236 21.920234833703805 16-17 21.849987672957187 28.504651866478454 27.677140737465688 21.96821972309867 18-19 21.78413494021738 28.526871679588595 27.649111221628342 22.039882158565682 20-21 21.9144392837238 28.476983245694804 27.70294827199202 21.90562919858937 22-23 21.859726737051655 28.500344301323068 27.696410795811943 21.943518165813337 24-25 21.78614482876973 28.447348261690315 27.67648739546346 22.090019514076495 26-27 21.97629164506453 28.319132183568847 27.752105261891508 21.952470909475117 28-29 22.013515590524793 28.41977784149659 27.66642710783233 21.900279460146287 30-31 21.960711690706994 28.420733151932854 27.66571239409853 21.95284276326162 32-33 21.956352644568188 28.48843989455637 27.568921755970972 21.986285704904464 34-35 21.98579743512593 28.578762727211743 27.524977475902823 21.910462361759503 36-37 22.022177071813587 28.473982863287578 27.532322751701656 21.971517313197182 38-39 22.03640058275352 28.49764625661252 27.51560128088023 21.950351879753732 40-41 22.017287297654637 28.621284656185896 27.45329381131501 21.90813423484446 42-43 22.026543194325992 28.57487779810427 27.43292093171498 21.965658075854762 44-45 21.984962423040898 28.59726036780725 27.445439036616836 21.972338172535007 46-47 22.025495891032904 28.64061730886644 27.368773605013242 21.96511319508741 48-49 22.049810310729224 28.636866830857404 27.361654773169676 21.951668085243696 50-51 22.06180476398454 28.611229129297516 27.35629088197939 21.97067522473855 52-53 22.021285448739743 28.732475714593438 27.23604206536135 22.010196771305473 54-55 22.088404852354294 28.70644881248544 27.224677409356605 21.980468925803656 56-57 22.032381202547608 28.732496943714242 27.240082674688065 21.995039179050085 58-59 22.015971092164673 28.82172293846131 27.202139159434374 21.960166809939643 60-61 22.059743145982292 28.81473759845803 27.17573590232168 21.949783353237997 62-63 21.98803808790562 28.870999014967957 27.176729750232102 21.96423314689432 64-65 22.056377596150444 28.973357393058063 27.115047942539718 21.85521706825178 66-67 21.985003574637968 28.99324707262166 27.10258450252383 21.91916485021655 68-69 21.94824863503443 29.067008635747733 27.092815111100794 21.89192761811704 70-71 21.95651523193926 29.13608108623536 27.00102458878636 21.906379093039018 72-73 21.96157656236964 29.107001923570774 26.99425844234429 21.9371630717153 74-75 22.007300130085003 29.152351454679813 27.00068053499377 21.839667880241407 76-77 21.964271214657117 29.245981659472932 26.940102434081247 21.849644691788708 78-79 21.975346700957196 29.24978831570672 26.894869143740486 21.879995839595594 80-81 21.921634212983683 29.267699647964367 26.91470502402996 21.89596111502199 82-83 21.960850167186827 29.356805371830852 26.84634571296402 21.835998748018305 84-85 21.91531794341555 29.403497374032465 26.825456138451226 21.855728544100756 86-87 21.91390721861225 29.44411551349585 26.810912471769775 21.831064796122128 88-89 21.910541896718584 29.497107294481715 26.786610407844343 21.805740400955358 90-91 21.882650593444207 29.510514389183882 26.780454876447735 21.826380140924176 92-93 21.88419424307683 29.575744192434524 26.737039580937406 21.803021983551243 94-95 21.862819596018475 29.628813866955788 26.729866207102344 21.778500329923393 96-97 21.839449110466823 29.647972831196267 26.688473940484496 21.824104117852407 98-99 21.818670975023817 29.707530883755446 26.632068938435594 21.841729202785146 100-101 21.837655670745658 29.808085655493432 26.56133502970888 21.79292364405203 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 121403.0 1 68075.5 2 11355.0 3 7258.0 4 6206.5 5 5446.0 6 4950.5 7 4848.0 8 4653.0 9 4352.0 10 4206.5 11 4603.5 12 7582.0 13 7363.0 14 4637.0 15 4827.5 16 5448.5 17 6266.0 18 7421.5 19 9178.0 20 11719.5 21 15491.0 22 19873.5 23 24881.5 24 30704.5 25 37937.0 26 47186.5 27 57555.0 28 67286.5 29 75887.0 30 86394.5 31 99035.0 32 111810.0 33 125854.0 34 139841.5 35 153136.0 36 165988.5 37 178432.0 38 190198.0 39 196555.0 40 204425.5 41 216967.5 42 226932.5 43 236181.5 44 244090.5 45 251577.5 46 257922.5 47 261016.0 48 266086.5 49 270111.5 50 268756.0 51 261244.0 52 248960.0 53 238905.5 54 226492.0 55 210286.5 56 193577.5 57 175084.0 58 156864.5 59 136695.0 60 113024.5 61 89302.0 62 69692.5 63 53484.5 64 39564.0 65 27324.5 66 17996.5 67 12541.5 68 7759.5 69 4040.0 70 2029.5 71 852.5 72 395.0 73 184.0 74 79.0 75 55.5 76 34.5 77 24.0 78 19.0 79 11.0 80 6.0 81 6.5 82 6.0 83 4.5 84 3.5 85 2.5 86 1.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.012779930725132987 2 0.018724084550776236 3 0.005873390089623687 4 3.6797142730172496E-4 5 0.0010189977986816998 6 0.0 7 0.0 8 0.008151982389453598 9 0.009468187879417462 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 1.0614560402934374E-4 24-25 0.005002996136583069 26-27 0.006977304371528862 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 2.122912080586875E-5 62-63 8.4916483223475E-5 64-65 2.2644395526259998E-4 66-67 2.1936758166064374E-4 68-69 2.8305494407824996E-4 70-71 6.368736241760624E-5 72-73 7.076373601956249E-6 74-75 2.122912080586875E-5 76-77 0.12645479626695819 78-79 0.055938733323464156 80-81 5.6610988815649995E-5 82-83 0.0023705851566553434 84-85 0.010628713150138287 86-87 0.11876277816163174 88-89 0.1354276379942387 90-91 0.14936101761649057 92-93 0.14718857092069 94-95 0.11417021169396213 96-97 1.2212476325992114 98-99 0.07613470358344729 100-101 0.06835069262129542 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 7065766.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 74.84495834919333 #Duplication Level Percentage of deduplicated Percentage of total 1 92.32747007186842 69.10245652015388 2 4.639202852333897 6.94441888512779 3 0.960366011127573 2.156356623084725 4 0.45287833682959977 1.355826410290534 5 0.26876180193481025 1.0057732935832513 6 0.20065987152944148 0.901102783618532 7 0.15597852534337311 0.817194436288535 8 0.11683377293266767 0.69955350951397 9 0.10084184899398416 0.6792753589029349 >10 0.7616183772067797 10.043628098941078 >50 0.014044028639424297 0.6626835902571464 >100 9.620659684883531E-4 0.12520553027688308 >500 4.7804411483666786E-5 0.01831894623438203 >1k 1.9121764574343134E-4 0.49242901713213333 >5k 4.7804411435857834E-5 0.29124262148191277 >10k+ 9.560882287171567E-5 4.704534375112254 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 104340 1.4766976432562302 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 27148 0.3842187810918165 No Hit AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 8140 0.11520336223984774 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 1.4152747203912499E-5 0.0 0.0 0.0 1.4152747203912499E-5 3 1.4152747203912499E-5 0.0 0.0 0.0 2.8305494407824997E-5 4 1.4152747203912499E-5 0.0 0.0 0.0 2.8305494407824997E-5 5 1.4152747203912499E-5 0.0 0.0 1.4152747203912499E-5 2.8305494407824997E-5 6 1.4152747203912499E-5 0.0 0.0 1.4152747203912499E-5 2.8305494407824997E-5 7 1.4152747203912499E-5 0.0 0.0 1.4152747203912499E-5 4.245824161173749E-5 8 1.4152747203912499E-5 0.0 0.0 1.4152747203912499E-5 4.245824161173749E-5 9 1.4152747203912499E-5 0.0 0.0 1.4152747203912499E-5 4.245824161173749E-5 10-11 1.4152747203912499E-5 7.076373601956249E-6 0.0 2.8305494407824997E-5 4.245824161173749E-5 12-13 3.5381868009781245E-5 1.4152747203912499E-5 0.0 2.8305494407824997E-5 5.6610988815649995E-5 14-15 4.245824161173749E-5 1.4152747203912499E-5 0.0 2.8305494407824997E-5 9.90692304273875E-5 16-17 6.368736241760625E-5 1.4152747203912499E-5 0.0 2.8305494407824997E-5 1.0614560402934375E-4 18-19 8.4916483223475E-5 4.245824161173749E-5 0.0 2.8305494407824997E-5 1.1322197763129999E-4 20-21 1.2029835123325624E-4 4.245824161173749E-5 0.0 2.8305494407824997E-5 1.1322197763129999E-4 22-23 1.556802192430375E-4 4.245824161173749E-5 0.0 2.8305494407824997E-5 1.4860384564108122E-4 24-25 1.556802192430375E-4 4.245824161173749E-5 0.0 4.245824161173749E-5 1.6275659284499373E-4 26-27 1.556802192430375E-4 5.6610988815649995E-5 0.0 4.245824161173749E-5 1.8398571365086248E-4 28-29 1.6275659284499373E-4 7.076373601956249E-5 0.0 4.245824161173749E-5 2.0521483445673123E-4 30-31 2.1936758166064374E-4 7.076373601956249E-5 0.0 4.245824161173749E-5 2.1229120805868747E-4 32-33 2.54749449670425E-4 7.784010962151873E-5 0.0 4.245824161173749E-5 2.405967024665125E-4 34-35 2.9720769128216244E-4 8.491648322347499E-5 0.0 4.245824161173749E-5 2.54749449670425E-4 36-37 3.6797142730172496E-4 8.491648322347499E-5 0.0 4.245824161173749E-5 2.689021968743375E-4 38-39 3.8920054810759374E-4 8.491648322347499E-5 0.0 4.245824161173749E-5 2.759785704762937E-4 40-41 4.599642841271562E-4 8.491648322347499E-5 0.0 4.245824161173749E-5 2.8305494407824996E-4 42-43 5.0949889934085E-4 9.199285682543124E-5 0.0 4.245824161173749E-5 2.972076912821625E-4 44-45 5.448807673506311E-4 1.273747248352125E-4 0.0 4.245824161173749E-5 3.0428406488411874E-4 46-47 6.014917561662813E-4 1.273747248352125E-4 0.0 4.245824161173749E-5 3.11360438486075E-4 48-49 6.793318657878E-4 1.273747248352125E-4 0.0 4.245824161173749E-5 3.11360438486075E-4 50-51 7.996302170210562E-4 1.4152747203912498E-4 0.0 4.245824161173749E-5 3.2551318568998746E-4 52-53 9.128521946523561E-4 1.6983296644694997E-4 0.0 4.245824161173749E-5 3.2551318568998746E-4 54-55 0.0010260741722836562 1.6983296644694997E-4 0.0 6.368736241760625E-5 3.325895592919437E-4 56-57 0.0011180670291090875 1.6983296644694997E-4 0.0 7.076373601956249E-5 3.467423064958562E-4 58-59 0.0012595945011482125 1.6983296644694997E-4 0.0 7.076373601956249E-5 3.538186800978125E-4 60-61 0.0013657401051775562 1.8398571365086248E-4 0.0 7.076373601956249E-5 3.538186800978125E-4 62-63 0.0015780313132362435 1.8398571365086248E-4 0.0 7.076373601956249E-5 3.538186800978125E-4 64-65 0.0018823153781203621 1.98138460854775E-4 0.0 7.076373601956249E-5 3.8212417450563744E-4 66-67 0.0021229120805868746 1.98138460854775E-4 0.0 7.076373601956249E-5 3.8212417450563744E-4 68-69 0.002299821420635781 1.98138460854775E-4 0.0 7.076373601956249E-5 4.1042966891346246E-4 70-71 0.002497959881490556 2.122912080586875E-4 0.0 7.076373601956249E-5 4.1042966891346246E-4 72-73 0.0027244038367531557 2.2644395526259998E-4 0.0 7.076373601956249E-5 4.175060425154187E-4 74-75 0.0031419098792685746 2.2644395526259998E-4 0.0 7.076373601956249E-5 4.2458241611737494E-4 76-77 0.0036160269105996435 2.2644395526259998E-4 0.0 7.076373601956249E-5 4.2458241611737494E-4 78-79 0.004295358776387443 2.2644395526259998E-4 0.0 7.076373601956249E-5 4.2458241611737494E-4 80-81 0.005130370861418281 2.405967024665125E-4 0.0 7.076373601956249E-5 4.387351633212875E-4 82-83 0.006198903275313675 2.405967024665125E-4 0.0 7.784010962151873E-5 4.458115369232437E-4 84-85 0.007854774698171436 2.54749449670425E-4 0.0 8.491648322347499E-5 4.5288791052519996E-4 86-87 0.010522567546108943 2.54749449670425E-4 0.0 8.491648322347499E-5 4.6704065772911244E-4 88-89 0.01390507412784403 2.689021968743375E-4 0.0 8.491648322347499E-5 4.882697785349812E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATGCCG 965 0.0 22.598772 48-49 GATCGGA 2210 0.0 21.67467 1 CTCGTAT 990 0.0 20.352045 44-45 ATCGGAA 2360 0.0 19.693184 2 TCTCGTA 990 0.0 18.675993 42-43 CGTACGC 130 3.3489778E-7 18.23654 20-21 CAACGCA 5655 0.0 18.192043 12-13 CGTCACG 290 5.3232725E-8 17.987675 3 ATCAACG 5785 0.0 17.783232 10-11 AGTGGTA 7055 0.0 16.668798 5 GTATCAA 7015 0.0 16.62724 9 ACACGTC 1395 0.0 16.482452 12-13 GGTATCA 7080 0.0 16.47587 8 ATGCCGT 1415 0.0 16.417006 48-49 TCAACGC 5425 0.0 16.166883 10-11 GTGGTAT 7380 0.0 15.998878 6 ACGCAGA 6505 0.0 15.814912 14-15 TCGGAAG 3045 0.0 15.729482 3 TCGTATG 1440 0.0 15.638153 44-45 TGGTATC 7550 0.0 15.575832 7 >>END_MODULE