SRR522150_1.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	SRR522150_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	35522682
Sequences flagged as poor quality	0
Sequence length	50
%GC	45

Per base quality graph

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
TCTCTGAGCGGGCTGGCAAGGCAGACCGATATCGTATGCCGTCTTCTGCT	515634	1.451562694506006	Illumina PCR Primer Index 1 (95% over 24bp)
TCTCTGAGCGGGCTGGCAAGGCAGACCGATCTCGTATGCCGTCTTCTGCT	174646	0.4916464359307104	Illumina Paired End PCR Primer 2 (96% over 29bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	124870	0.351521881146249	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	109519	0.30830723873833626	No Hit
CTTATACACATCTCTGAGCGGGCTGGCAAGGCAGACCGATATCGTATGCC	73032	0.20559258447884085	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	64405	0.18130669300251595	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	36624	0.10310032333707235	No Hit
CATCTCTGAGCGGGCTGGCAAGGCAGACCGATATCGTATGCCGTCTTCTG	36512	0.10278503182839628	Illumina Paired End PCR Primer 2 (96% over 26bp)

Adapter graph

Kmer graph

Sequence	Count	Obs/Exp Max	Max Obs/Exp Position
CGTCTTC	95255	36.055714	40
GCCGTCT	97755	35.18698	38
CCGTCTT	97845	35.172844	39
TTCTGCT	102905	33.954365	44
TATGCCG	102415	33.931183	35
ATGCCGT	102885	33.55966	36
TGCCGTC	103265	33.363396	37
GTCTTCT	103620	33.14168	41
CTTCTGC	106270	32.95953	43
GGTATCA	55480	31.338009	1
TCTTCTG	110905	30.938091	42
TATCGTA	79670	30.705881	30
GACCGAT	113255	30.700369	24
ACCGATA	81295	30.677507	25
ATATCGT	80080	30.583738	29
ATCGTAT	81360	30.551327	31
TCGTATG	113315	30.5085	32
CCGATAT	81265	30.502167	26
CGATATC	81445	30.468428	27
TGAGCGG	122130	30.432053	5