##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR522150_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	35522682
Sequences flagged as poor quality	0
Sequence length	50
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	38.26506284069429	40.0	38.0	40.0	35.0	40.0
2	37.94718982648889	40.0	38.0	40.0	34.0	40.0
3	37.87338095698968	39.0	38.0	40.0	33.0	40.0
4	37.805144893057346	40.0	38.0	40.0	34.0	40.0
5	37.90699066585119	39.0	38.0	40.0	35.0	40.0
6	38.07959444053239	40.0	38.0	40.0	35.0	40.0
7	38.0371002392218	40.0	38.0	40.0	35.0	40.0
8	37.98040376005393	39.0	38.0	40.0	34.0	40.0
9	37.891935749671156	40.0	38.0	40.0	33.0	40.0
10	37.906214007151824	39.0	38.0	40.0	33.0	40.0
11	38.17211904213764	40.0	38.0	40.0	35.0	40.0
12	38.06691814542607	39.0	38.0	40.0	35.0	40.0
13	38.040161466411796	39.0	38.0	40.0	35.0	40.0
14	37.97798555863547	39.0	38.0	40.0	34.0	40.0
15	38.007531807423774	39.0	38.0	40.0	34.0	40.0
16	38.01764385358065	39.0	38.0	40.0	35.0	40.0
17	37.938547714387106	39.0	38.0	40.0	34.0	40.0
18	37.90429613394619	39.0	38.0	40.0	34.0	40.0
19	37.90721258040145	39.0	38.0	40.0	34.0	40.0
20	37.837433812007774	39.0	38.0	40.0	33.0	40.0
21	37.819252555310996	39.0	38.0	40.0	34.0	40.0
22	37.73989162755222	39.0	38.0	40.0	33.0	40.0
23	37.704554008619056	39.0	38.0	40.0	33.0	40.0
24	37.63409018496971	39.0	38.0	40.0	33.0	40.0
25	37.557534647862454	39.0	38.0	40.0	33.0	40.0
26	37.31319389115946	39.0	38.0	40.0	33.0	40.0
27	37.13988667860158	39.0	37.0	40.0	33.0	40.0
28	37.06527741908677	39.0	37.0	40.0	33.0	40.0
29	37.119194406548466	39.0	37.0	40.0	33.0	40.0
30	36.951829228434946	39.0	37.0	40.0	32.0	40.0
31	36.44000658508837	39.0	36.0	40.0	31.0	40.0
32	36.5832756941044	39.0	36.0	40.0	31.0	40.0
33	36.602508082019256	39.0	36.0	40.0	31.0	40.0
34	36.567157907727804	39.0	36.0	40.0	31.0	40.0
35	36.38456291110001	39.0	36.0	40.0	30.0	40.0
36	36.69522929040099	39.0	37.0	40.0	32.0	40.0
37	36.53507226734738	39.0	36.0	40.0	31.0	40.0
38	36.46915663631479	39.0	36.0	40.0	31.0	40.0
39	36.395173596408064	39.0	36.0	40.0	31.0	40.0
40	36.2896646430019	39.0	36.0	40.0	31.0	40.0
41	36.21907124017269	39.0	36.0	40.0	31.0	40.0
42	36.09727337029338	39.0	36.0	40.0	30.0	40.0
43	35.86895792384145	39.0	36.0	40.0	30.0	40.0
44	35.9354509324493	39.0	36.0	40.0	30.0	40.0
45	35.737045699421	39.0	36.0	40.0	30.0	40.0
46	35.366980511212525	39.0	36.0	40.0	29.0	40.0
47	35.201736766384926	39.0	35.0	40.0	28.0	40.0
48	35.00967319978824	39.0	35.0	40.0	27.0	40.0
49	34.887225970156194	39.0	35.0	40.0	27.0	40.0
50	34.82480813244901	39.0	35.0	40.0	27.0	40.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1	1	-0.08190585345055723
1	2	0.02409815181412256
1	3	0.11071448269851203
1	4	0.2453521906501024
1	5	0.07401667136624468
1	6	0.02958210186790211
1	7	0.05817183164743511
1	8	0.05379365180511542
1	9	0.10455513783395531
1	10	0.07452134965932089
1	11	-0.06140739403404183
1	12	-0.07689564054884812
1	13	-0.0504386660938394
1	14	0.00351332953689365
1	15	-0.1358843050019658
1	16	-0.13738897735931488
1	17	-0.12399432879843175
1	18	-0.07894673209879954
1	19	-0.22315682108227008
1	20	-0.09163255304373052
1	21	-0.22711244687450716
1	22	-0.15539403089870518
1	23	-0.21635388876669026
1	24	-0.16717922849554157
1	25	-0.14184591577961214
1	26	-0.24898524039921455
1	27	-0.0682091057279095
1	28	-0.15623918041763574
1	29	-5.5015374400113615E-5
1	30	0.039451017109811914
1	31	0.2003591221209149
1	32	0.15114915456474876
1	33	0.03242941853820014
1	34	0.0961460624884225
1	35	0.06158062367892825
1	36	0.06656153004883691
1	37	0.04252962098279767
1	38	0.12139268597678665
1	39	0.04794085679893101
1	40	0.06653378788303144
1	41	0.07107657873141449
1	42	0.021976824600137945
1	43	0.16792363204462646
1	44	-0.020957659920270544
1	45	0.061193255111248845
1	46	0.09707302756054759
1	47	0.11303796126211552
1	48	0.17197621682922204
1	49	0.08160763437528828
1	50	0.18392621771199202
2	1	0.05050130263118291
2	2	0.15954551987368149
2	3	0.1717569526146292
2	4	0.2454374961762369
2	5	0.2169809751796592
2	6	0.12441992015655501
2	7	0.10306953301699906
2	8	0.10233549026875721
2	9	0.29663100413061727
2	10	0.11379347007019902
2	11	7.666105422288183E-4
2	12	-0.08610905656163936
2	13	-0.03568812497417895
2	14	0.010068522939697289
2	15	-0.0988931979615586
2	16	-0.18610543436269467
2	17	-0.1487772604739206
2	18	-0.0672434506367594
2	19	-0.11167604311028612
2	20	-0.19056641026568855
2	21	-0.1269219273006854
2	22	-0.24239272305252513
2	23	-0.17477977501157227
2	24	-0.17123685309175585
2	25	-0.2358714418014003
2	26	-0.17716296473319915
2	27	-0.1777126650281886
2	28	-0.3086323833739897
2	29	-0.19767615152236573
2	30	-0.170023676157534
2	31	0.15591644986509579
2	32	0.005880755482479572
2	33	-0.04165313042735619
2	34	-0.021781718677353012
2	35	-0.10474476396672827
2	36	-0.1258586147659173
2	37	0.02036159024360984
2	38	-0.011094135779757153
2	39	-0.07838522733335651
2	40	-0.13602886887181853
2	41	-0.08170105530859928
2	42	-0.11105333186253574
2	43	-0.005179315894395131
2	44	-0.06824344389977455
2	45	-0.12093746330720023
2	46	-0.011491983127896788
2	47	0.0036063634255540933
2	48	0.028515612074436092
2	49	-0.23093050217394762
2	50	-0.10588114431225648
3	1	0.07113748470815295
3	2	0.10047676779455372
3	3	0.15635726618970125
3	4	0.33492585257573637
3	5	0.23766202156011929
3	6	0.06535892617634431
3	7	0.057595867219561114
3	8	0.07478422492904713
3	9	0.13538622881983287
3	10	0.07423387338562293
3	11	-0.1351616378740701
3	12	-0.08980920505383239
3	13	0.0020557241584882036
3	14	-0.15596639907758458
3	15	-0.27515524467312247
3	16	-0.16390764077570452
3	17	-0.10737045542748547
3	18	-0.20020454148178146
3	19	-0.1635249884502059
3	20	-0.10710661151295398
3	21	-0.09261521311434961
3	22	-0.2741458186436887
3	23	-0.21563780036758118
3	24	-0.3183782959492447
3	25	-0.2342682153719977
3	26	-0.27787725744376246
3	27	-0.2921711615569862
3	28	-0.3052276420751454
3	29	-0.08012952221250202
3	30	-0.14709106225154756
3	31	-0.07092724264933281
3	32	-0.06582061219383206
3	33	-0.11263144881251463
3	34	-0.14455528550936947
3	35	-0.27117967635850704
3	36	-0.19893143847362893
3	37	-0.12595075696861358
3	38	-0.24604639773201598
3	39	-0.2315392659507225
3	40	-0.1411201545401326
3	41	-0.1453317077491718
3	42	-0.20481575468901525
3	43	-0.07853009582346004
3	44	-0.22280961026551438
3	45	-0.1992239989736504
3	46	-0.15234425598571022
3	47	-0.05932095369065138
3	48	-0.04461282775321962
3	49	-0.12944265565653978
3	50	-0.261948481225005
4	1	0.0751415766126442
4	2	0.0481044549645091
4	3	0.06417784477512356
4	4	0.27640254557142896
4	5	0.19980860823218904
4	6	0.0288123450561244
4	7	0.0912059140406285
4	8	0.017262435979965574
4	9	0.17026514163293882
4	10	0.1169416182487737
4	11	-0.01481302460777556
4	12	-0.051405523047691304
4	13	-0.00892940075738835
4	14	0.01857838103708076
4	15	-0.055085193189889026
4	16	-0.09138301293514672
4	17	-0.10795071250008448
4	18	-0.07298927152848478
4	19	-0.09555686595508206
4	20	-0.14605900822317608
4	21	-0.11471796664844192
4	22	-0.262328873824039
4	23	-0.255972958236498
4	24	-0.18050695031670472
4	25	-0.18319105123084256
4	26	-0.1898721994720134
4	27	-0.0971947974426044
4	28	-0.10448440774570145
4	29	-0.011189770866700144
4	30	-0.005889376910396038
4	31	0.05554323430192909
4	32	0.0969330081086639
4	33	-0.025792031534436433
4	34	-0.10634827694021709
4	35	0.04890081946663827
4	36	-0.09021254485713115
4	37	0.07575006910924742
4	38	-0.02063156185815984
4	39	0.041058059862351115
4	40	-0.11020743354241347
4	41	-0.03130879850568391
4	42	-0.23008541614179023
4	43	0.07363867319416073
4	44	-0.1052953796865097
4	45	-0.05154574186592953
4	46	-0.05007423667861843
4	47	-0.010621955537075678
4	48	0.0068804899639971495
4	49	-0.01735933061005568
4	50	-0.2600789154827581
5	1	-0.2800094387985368
5	2	-0.427152398118686
5	3	-0.41442008194407265
5	4	-0.2173300728067531
5	5	-0.2837245283576806
5	6	-0.2724354871985142
5	7	-0.2021851809915418
5	8	-0.16876777600618453
5	9	0.018476229430262947
5	10	-0.1293206067923407
5	11	-0.21013281712888698
5	12	-0.2471432454109035
5	13	-0.1786528788346402
5	14	-0.16872596382083316
5	15	-0.2852955152440728
5	16	-0.33035506935897274
5	17	-0.2738925656051592
5	18	-0.2706340094924542
5	19	-0.29375542184815373
5	20	-0.3161636988212777
5	21	-0.29053445841741166
5	22	-0.35178527212198674
5	23	-0.3738380350132715
5	24	-0.3399754310801839
5	25	-0.3715905186504713
5	26	-0.34259891242291474
5	27	-0.24364350929494094
5	28	-0.3772238377395638
5	29	-0.2116973338575292
5	30	-0.21590252979274993
5	31	-0.20703435813572924
5	32	-0.2663981911964868
5	33	-0.26873686959851995
5	34	-0.40614454332661865
5	35	-0.3888586081853518
5	36	-0.3482951726884167
5	37	-0.22626387322290498
5	38	-0.24259894137155413
5	39	-0.35010768273274806
5	40	-0.3776394041813802
5	41	-0.2817835855462647
5	42	-0.4443799502413768
5	43	-0.2639092147940829
5	44	-0.4816721214154711
5	45	-0.5303595814247046
5	46	-0.44623522096903656
5	47	-0.42628429980570814
5	48	-0.49171019339036803
5	49	-0.5263286982272319
5	50	-0.6035265327733796
6	1	0.12498285799036779
6	2	0.10152635081112749
6	3	0.11154422485351034
6	4	0.25304001115593167
6	5	0.1826893838933259
6	6	0.06546354435479174
6	7	0.08468248991415095
6	8	0.07599656225969653
6	9	0.23959932876572765
6	10	0.12594862933386253
6	11	-0.04281015068741567
6	12	-0.04561553388716533
6	13	-0.03838101568291563
6	14	-0.07611983250546217
6	15	-0.18142839191700944
6	16	-0.16145794853304096
6	17	-0.17146310378323903
6	18	-0.07245209941070385
6	19	-0.10916021523601671
6	20	-0.05577459690363895
6	21	-0.044261026420485905
6	22	-0.10828851499799441
6	23	-0.10330803478044714
6	24	-0.0634647073249468
6	25	-0.10155893310199104
6	26	-0.026297319700638866
6	27	-0.046959757094583665
6	28	-0.07899944928712443
6	29	0.06722451135919272
6	30	0.045068447241398246
6	31	0.08536328520692393
6	32	0.07501557014260385
6	33	0.09146744611592084
6	34	0.07530312641122805
6	35	-0.013410812165815855
6	36	0.046967262469387094
6	37	0.05281988995260889
6	38	-0.005194378155955803
6	39	0.054513940168263275
6	40	-0.12785316389343393
6	41	-0.0032995858138633594
6	42	0.02325653718168752
6	43	0.11027682686290063
6	44	0.06608542891658686
6	45	-0.012601604994607385
6	46	0.023991338740898982
6	47	0.15586330133600512
6	48	0.22463692629880683
6	49	0.09273383557221138
6	50	0.041605164407101825
7	1	0.17364333495308415
7	2	0.20378331475733802
7	3	0.20657726636074614
7	4	0.40432718735617357
7	5	0.29455866300404665
7	6	0.16268424275504145
7	7	0.16959925378283458
7	8	0.21088542573814806
7	9	0.3129150285991784
7	10	0.20384805272305329
7	11	-0.016299617152313317
7	12	0.05026405510488985
7	13	0.08587367924959466
7	14	0.09184630364643454
7	15	-0.14920928375642717
7	16	-0.03605408934128462
7	17	-0.05646774171064095
7	18	0.01488465116533888
7	19	-0.2197323992535516
7	20	-0.04944963111655909
7	21	-0.16684133434788606
7	22	0.005131391081540926
7	23	-0.16453577453256685
7	24	-0.06904797530548734
7	25	-0.03878378766149382
7	26	-0.2163964904683695
7	27	-0.06188362698075167
7	28	-0.09411510939269618
7	29	-0.18927103900383457
7	30	-0.18582299509548506
7	31	0.053134132597797645
7	32	-0.06638379484621026
7	33	0.009969859638573553
7	34	-0.14821980393641354
7	35	-0.1014812776477001
7	36	0.01185995354902758
7	37	0.04175777201643882
7	38	-0.009254712149342481
7	39	-0.029512698031680884
7	40	0.05139283961786845
7	41	0.06004915531188715
7	42	-0.21806441268871168
7	43	0.16139772355790427
7	44	-0.08297952319471591
7	45	-0.001356633496662596
7	46	0.12187291009299628
7	47	0.06471646467351633
7	48	0.25040133802809095
7	49	0.059714305940268275
7	50	-0.06919597142372425
8	1	0.17321296182894486
8	2	0.2511541561572912
8	3	0.2456588195420295
8	4	0.3850023348774627
8	5	0.35575408060820735
8	6	0.19081592255610502
8	7	0.21989453880765808
8	8	0.18079773814289268
8	9	0.34950520224134607
8	10	0.2003235429114696
8	11	0.09085995717888551
8	12	-0.023031048730956627
8	13	0.05710200881146221
8	14	0.07764867691247446
8	15	-0.0051140739996569096
8	16	-0.0736419872112748
8	17	-0.10236720217027795
8	18	-0.12879155541913434
8	19	0.003673827422808529
8	20	-0.11803063753971088
8	21	0.015645925354533574
8	22	-0.05375883450896879
8	23	-0.05934134886724962
8	24	-0.0438664931290802
8	25	-0.012612940764242353
8	26	0.04112641772082526
8	27	-0.021773293975819286
8	28	-0.17159595839934383
8	29	-0.0857522937651396
8	30	0.017473275529638954
8	31	0.02336731570512285
8	32	-0.06287433908455142
8	33	-0.0056561656744023026
8	34	-0.03988364223323515
8	35	-0.1927363121375052
8	36	-0.12737793354909144
8	37	-0.03602983566200635
8	38	-0.005204435171620503
8	39	-0.16326139401066797
8	40	-0.18052657460338395
8	41	-0.02288221091218645
8	42	-0.0848615200386007
8	43	-0.10427418867535465
8	44	-0.029982414112680544
8	45	-0.09970075080698848
8	46	-0.0599280850209567
8	47	0.05204798692516732
8	48	0.043773260749162546
8	49	-0.1539763644351595
8	50	-0.1257105835936443
9	1	0.16972822188252934
9	2	0.3386338912505451
9	3	0.18554125932345755
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115	6	0.0559799036145705
115	7	0.058661594943274054
115	8	0.04066294083732913
115	9	0.18968332989960857
115	10	0.1382828146615367
115	11	-0.007428370703379983
115	12	-0.0569997388207355
115	13	0.06296204865866173
115	14	0.04375798042208601
115	15	0.024324388848675937
115	16	-0.031611053235685915
115	17	-0.020599888363797447
115	18	-0.03410627599515692
115	19	-0.10891598503258848
115	20	-0.09033716375950007
115	21	-0.1395019815045515
115	22	-0.1188000699792795
115	23	-0.041888338370632994
115	24	-0.12387144062962108
115	25	-0.27143944256744845
115	26	-0.13209912595217332
115	27	-0.06349909215344951
115	28	-0.06871925420152536
115	29	-0.10870079613565764
115	30	-0.08064965928981849
115	31	-0.11929922740911536
115	32	-0.018244115966616903
115	33	-0.045230357952362965
115	34	-0.04970020249789542
115	35	-0.021073363038091486
115	36	-0.03759730206036238
115	37	-0.10009968291371507
115	38	-0.05694530492298355
115	39	0.025929999954215077
115	40	-0.07275695636825219
115	41	-0.011966673137699502
115	42	-0.07438298225913798
115	43	-0.012268669992408832
115	44	-0.041998536168875944
115	45	-0.034099743688088324
115	46	-0.12983948799476508
115	47	-0.07735778132908422
115	48	-0.09504923954937539
115	49	-0.06543509232955103
115	50	-0.12166540775275081
116	1	-0.17333383256378454
116	2	-0.4560325982147475
116	3	-0.3705131444500722
116	4	-0.17471767854747355
116	5	-0.3702394929318302
116	6	-0.2960351186705026
116	7	-0.19738311393236785
116	8	-0.23670601330822194
116	9	-0.04232850594030424
116	10	-0.1138841421357526
116	11	-0.1694642229197285
116	12	-0.22548304253422202
116	13	-0.15763171304399037
116	14	-0.12402187460943992
116	15	-0.22800330966362736
116	16	-0.25741015406110535
116	17	-0.2765113731786002
116	18	-0.18849226644510253
116	19	-0.30357189134590357
116	20	-0.22728740211283593
116	21	-0.21034287677628072
116	22	-0.2029989448108367
116	23	-0.26710997901695066
116	24	-0.27813441720449816
116	25	-0.25376746576229436
116	26	-0.28066167608825765
116	27	-0.2663521849149575
116	28	-0.2743402419466605
116	29	-0.1548022397155151
116	30	-0.1571482769538335
116	31	-0.07617651889302834
116	32	-0.25212134728452185
116	33	-0.19671349323363074
116	34	-0.33411974059501404
116	35	-0.25489455356373014
116	36	-0.2124451936156433
116	37	-0.22377353186338667
116	38	-0.22621345627897682
116	39	-0.19466485600174366
116	40	-0.2740910642064591
116	41	-0.20374566542645312
116	42	-0.3365480061287869
116	43	-0.24778519434971003
116	44	-0.2928980659546525
116	45	-0.26259335026599473
116	46	-0.32758612816186883
116	47	-0.3200111316510217
116	48	-0.2615446571369944
116	49	-0.45710699255575094
116	50	-0.6674571311257367
117	1	0.09731317956362773
117	2	0.12345383162885781
117	3	0.11861131493851218
117	4	0.2221645930259939
117	5	0.1835836400610944
117	6	0.1097002546993977
117	7	0.04144944314097643
117	8	0.08297113479856222
117	9	0.19692778454545845
117	10	0.14621218127145852
117	11	-0.013729237532608352
117	12	-0.09746180934314452
117	13	0.0029977343065752393
117	14	-0.09376853193933243
117	15	-0.07657792966932675
117	16	-0.09541647407997544
117	17	-0.1266104546110114
117	18	-0.2514994671550781
117	19	-0.10569579136297591
117	20	-0.10684580391195908
117	21	-0.10049907121589996
117	22	-0.18428432840380538
117	23	-0.11344978158565766
117	24	-0.05895276969932439
117	25	-0.1643562072883853
117	26	-0.10701822499882496
117	27	-0.06581247369690146
117	28	-0.09486913703955224
117	29	-0.005760423197280318
117	30	-0.07049524044447253
117	31	0.07362735031792056
117	32	0.0338074692397754
117	33	-0.017845443035646724
117	34	-0.014311539486058678
117	35	0.11869994304253595
117	36	0.07391267223879794
117	37	0.036704924343816
117	38	0.08876463945686908
117	39	-0.032705399423697656
117	40	0.05788871290791775
117	41	0.10837407386499365
117	42	0.1488973041260806
117	43	0.10448670648988667
117	44	0.08057548990312569
117	45	0.07495405128522492
117	46	-0.06103267506905041
117	47	-0.0153253553608792
117	48	0.07259344134967449
117	49	0.05995123039225092
117	50	-0.11466332198373408
119	1	0.032501150234963916
119	2	0.11032740911589656
119	3	0.10143618244113384
119	4	0.30207023530683585
119	5	0.22621322530618215
119	6	0.10622937154886358
119	7	0.09900402123508201
119	8	0.08568063886773558
119	9	0.2916106679391959
119	10	0.11547086494388026
119	11	-0.07653769246481801
119	12	-0.07674580732511771
119	13	-0.030256884600078138
119	14	0.04076199375218437
119	15	-0.09553224225518164
119	16	-0.11731567723145986
119	17	-0.17182683711293123
119	18	-0.1353743824140139
119	19	-0.1770800232054981
119	20	-0.21763197118391986
119	21	-0.23845695228065011
119	22	-0.16510121089646645
119	23	-0.2127453932955774
119	24	-0.21889459179919157
119	25	-0.2985685870580568
119	26	-0.25837681259653067
119	27	-0.2072145104932872
119	28	-0.4068442484860739
119	29	-0.26231109969540967
119	30	-0.3334050777504629
119	31	-0.25496484713430334
119	32	-0.327591186046682
119	33	-0.24838909009964993
119	34	-0.23395597799321877
119	35	-0.3301106988585829
119	36	-0.3454672966902308
119	37	-0.23193782686326614
119	38	-0.2835038273633472
119	39	-0.2547195958596902
119	40	-0.2651711539137409
119	41	-0.23073818814138747
119	42	-0.20241365563008173
119	43	-0.13670358914210112
119	44	-0.19844703849808099
119	45	-0.3693394244615078
119	46	-0.20160769003295087
119	47	-0.17847746636894612
119	48	-0.11121096667800856
119	49	-0.15507992773002144
119	50	-0.20850564386893922
120	1	0.01926688488563144
120	2	0.19980064088802862
120	3	0.1906217468081337
120	4	0.34857862679456986
120	5	0.25975992168241646
120	6	0.1433777483914156
120	7	0.07304126259492705
120	8	0.033734180728849594
120	9	0.25471265018014577
120	10	0.11788651343542256
120	11	-0.007705482330955249
120	12	-0.05984527904484338
120	13	0.029665845757207876
120	14	-0.11098937127322728
120	15	-0.14261821638121575
120	16	-0.19694898638153546
120	17	-0.1774252587545746
120	18	-0.16598919318651895
120	19	-0.16076180312987987
120	20	-0.105879862440041
120	21	-0.08792800129432266
120	22	-0.20511623918991972
120	23	-0.1471459245861837
120	24	-0.1486641615058204
120	25	-0.21285234512139795
120	26	-0.1443023914994228
120	27	-0.06524505874419617
120	28	-0.08951798802717548
120	29	0.025909711931738855
120	30	0.028505923820574708
120	31	0.3004795192244387
120	32	0.16692807247718378
120	33	0.023710157459191805
120	34	0.12232854130145654
120	35	0.12943725554711705
120	36	-0.012722661910046895
120	37	-0.021194892175472546
120	38	0.0021414935313543992
120	39	-0.05162990280389579
120	40	0.004358823722967031
120	41	0.15803883339801672
120	42	0.1283095457740373
120	43	0.27419529303998047
120	44	0.0962055956215977
120	45	0.13294946902382776
120	46	0.051965388103525356
120	47	0.15325253456445864
120	48	0.20887198856370048
120	49	0.13454904891760577
120	50	0.13858205574215532
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	29686.0
3	1969.0
4	3018.0
5	3333.0
6	4851.0
7	7945.0
8	8720.0
9	10336.0
10	14793.0
11	18917.0
12	23690.0
13	30089.0
14	37079.0
15	43076.0
16	50165.0
17	58529.0
18	67710.0
19	79243.0
20	102385.0
21	87179.0
22	101711.0
23	128600.0
24	161904.0
25	166883.0
26	224308.0
27	278019.0
28	335127.0
29	368731.0
30	452040.0
31	554271.0
32	686499.0
33	853508.0
34	1164691.0
35	1630555.0
36	2323846.0
37	3655907.0
38	5587031.0
39	1.6051279E7
40	115059.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.56386992260871	19.566458833466825	13.055634768200767	23.814036475723693
2	17.911312778192702	21.768871357190495	34.62292756904303	25.69688829557377
3	18.333472822087206	24.88728787891712	33.14729108758072	23.631948211414954
4	12.371168933697067	16.144383467442015	36.32470937864433	35.15973822021659
5	13.095019120459428	38.069369311697805	36.625694535114214	12.209917032728553
6	32.76673478652315	36.39706314967997	16.9454294019804	13.89077266181647
7	26.91012745039916	34.02878757859556	21.7716725330593	17.289412437945987
8	29.86793058024166	32.54850239066972	20.210270159218272	17.373296869870355
9	26.235715691457894	12.377643853145683	19.462943526736883	41.92369692865954
10	17.91040158510554	26.968709738752274	34.58311227738942	20.537776398752776
11	36.10021924303626	20.885865618412396	22.90332606952308	20.11058906902827
12	21.992691880641217	26.07507507456785	31.308215973106986	20.624017071683944
13	29.8146270154525	19.834165030522588	25.39803737029645	24.95317058372846
14	20.6875333590071	20.31848702686512	29.91071046521317	29.08326914891461
15	25.884050415927433	28.04314248624885	23.658127770432074	22.414679327391642
16	24.676121020366647	26.402606650027156	25.71502343207081	23.206248897535385
17	20.973033511377324	27.054105880856632	27.30839411280939	24.664466494956656
18	21.512824864011986	27.919024156354066	28.17505524988171	22.39309572975224
19	21.752823856331958	27.649468109199514	28.983077848641248	21.614630185827274
20	25.029393902046905	25.21775528497658	28.49668918505403	21.25616162792249
21	25.048937170654987	25.257905610427212	28.106889956697696	21.586267262220105
22	22.429400296458862	25.000952209767096	28.47931185285101	24.090335640923033
23	22.097970708223404	27.553504067138718	28.17730776189543	22.171217462742447
24	23.82498644696691	25.54169271070205	28.621784696503695	22.011536145827343
25	22.2522016781813	27.791634231078337	28.280310719421347	21.67585337131901
26	22.13098348677448	25.32418644174633	28.36159776408899	24.1832323073902
27	21.498709587328293	25.404474692888098	28.44945187750845	24.64736384227516
28	23.973299358863883	25.618774492486164	28.566013684015168	21.84191246463478
29	21.392066386904368	27.924510981265104	28.737086988459282	21.946335643371242
30	21.90249887603762	25.877372552086936	30.502766517155717	21.71736205471973
31	22.19892391898541	27.362056967859612	28.405475086839115	22.033544026315866
32	21.27434709365805	25.744547665705316	30.86447337303216	22.116631867604468
33	21.55278726103786	25.95998201737032	28.461642808772524	24.025587912819294
34	24.233468070061214	25.19475246563242	28.775127732377793	21.79665173192857
35	21.637954375228034	25.779916310405383	30.903303631313467	21.67882568305312
36	21.838080414174815	28.364437293835937	27.98819697598642	21.809285316002825
37	21.781796733846363	25.53669649001598	30.806819449857052	21.8746873262806
38	24.319449894759877	26.278072547859075	27.936138355307598	21.466339202073446
39	21.567152296375337	26.21077243891105	28.415611839810236	23.806463424903377
40	21.672250107061345	25.508145484884388	28.742927899812752	24.07667650824152
41	23.84994749225677	25.95775792360654	28.127867738750435	22.06442684538626
42	21.315003535085083	25.931926544951057	30.795597927517456	21.957471992446408
43	21.77627461559861	25.707578617748105	28.162339252006124	24.353807514647162
44	21.853789106152842	25.903753878240014	30.56376037140544	21.678696644201704
45	21.55519540409016	25.936872075748685	30.523953772462693	21.983978747698462
46	21.86731447597158	26.3825693143587	27.702951938060032	24.047164271609685
47	21.57276535180289	26.297941419424554	30.41941214061201	21.70988108816055
48	24.3367050620493	26.27093520484366	27.10455333618472	22.28780639692232
49	21.68754041116322	26.209942365804178	27.771768890710185	24.330748332322415
50	21.778136039274692	26.214807163473992	29.62079357997314	22.38626321727818
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	17341.0
1	15389.0
2	13437.0
3	30186.5
4	46936.0
5	48070.0
6	49204.0
7	38853.5
8	28503.0
9	38960.0
10	49417.0
11	75314.0
12	101211.0
13	167348.5
14	233486.0
15	234284.5
16	235083.0
17	307163.0
18	379243.0
19	366707.0
20	354171.0
21	392952.0
22	431733.0
23	481460.0
24	531187.0
25	587185.5
26	643184.0
27	723063.0
28	802942.0
29	883974.5
30	965007.0
31	1063810.5
32	1162614.0
33	1248347.5
34	1334081.0
35	1427429.5
36	1520778.0
37	1601429.0
38	1682080.0
39	1739627.5
40	1797175.0
41	1840272.5
42	1883370.0
43	1913879.0
44	1944388.0
45	1952999.0
46	1961610.0
47	1948064.0
48	1934518.0
49	1921065.0
50	1907612.0
51	1885131.0
52	1862650.0
53	1884925.5
54	1907201.0
55	1867048.0
56	1826895.0
57	2002971.0
58	2179047.0
59	1903085.0
60	1627123.0
61	1390110.5
62	1153098.0
63	1010670.5
64	868243.0
65	767285.0
66	666327.0
67	574086.0
68	481845.0
69	414367.0
70	346889.0
71	289578.5
72	232268.0
73	194204.5
74	156141.0
75	123793.0
76	91445.0
77	72538.5
78	53632.0
79	41580.5
80	29529.0
81	22299.5
82	15070.0
83	11512.5
84	7955.0
85	5986.5
86	4018.0
87	3048.5
88	2079.0
89	1328.0
90	577.0
91	372.5
92	168.0
93	119.5
94	71.0
95	53.0
96	35.0
97	29.5
98	24.0
99	32.5
100	41.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.05769271588220732
2	0.00279258193398798
3	2.955857893838084E-4
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	1.3794003504577723E-4
10	0.0
11	8.44530826810881E-6
12	0.0
13	3.884841803330053E-4
14	1.1823431575352334E-4
15	5.348695236468913E-5
16	0.0
17	0.0
18	0.0012217545961197413
19	1.9987229567857516E-4
20	2.252082204829016E-5
21	2.618045563113731E-4
22	1.323098295337047E-4
23	8.13564696494482E-4
24	0.0031332093674683683
25	0.017279100716550623
26	0.09462404893864715
27	0.08926690839391012
28	0.11580488207506405
29	0.15201554882595858
30	0.19700933617568628
31	0.2304330511981049
32	0.22564737651284325
33	0.24283920904395676
34	0.31012579511873567
35	0.320862597030258
36	0.3499060121642842
37	0.400729877321763
38	0.4082321261665997
39	0.41062496350923056
40	0.4497914881539632
41	0.46728453667997255
42	0.5380843709942847
43	0.4975722272321668
44	0.5266100121606809
45	0.5418650539956414
46	0.6196378978366555
47	0.645052645518151
48	0.6931289703857383
49	0.6931599365160547
50	0.7453294207909189
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	3.5522682E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.79665267230946
#Duplication Level	Percentage of deduplicated	Percentage of total
1	65.46282413606554	22.778871544111492
2	14.11865178534438	9.825636447518207
3	6.627808249087605	6.918766248665068
4	3.7665385423846987	5.242517337449085
5	2.4090115030189225	4.191276827707381
6	1.598024074413348	3.3363533207610057
7	1.116369594630972	2.7192147526810593
8	0.8422502089862082	2.3445990388218516
9	0.6223203736182961	1.9489199302527935
>10	3.0967895782399215	18.707029604930366
>50	0.1899669905930771	4.586058132453438
>100	0.1354363368785093	9.042003442275279
>500	0.010312730444171025	2.4234086073939993
>1k	0.0034618251404375383	1.9618982390806146
>5k	9.685703022393243E-5	0.23915090078105802
>10k+	1.3721412355958412E-4	3.734295625117244
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TCTCTGAGCGGGCTGGCAAGGCAGACCGATATCGTATGCCGTCTTCTGCT	515634	1.451562694506006	Illumina PCR Primer Index 1 (95% over 24bp)
TCTCTGAGCGGGCTGGCAAGGCAGACCGATCTCGTATGCCGTCTTCTGCT	174646	0.4916464359307104	Illumina Paired End PCR Primer 2 (96% over 29bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	124870	0.351521881146249	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	109519	0.30830723873833626	No Hit
CTTATACACATCTCTGAGCGGGCTGGCAAGGCAGACCGATATCGTATGCC	73032	0.20559258447884085	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	64405	0.18130669300251595	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	36624	0.10310032333707235	No Hit
CATCTCTGAGCGGGCTGGCAAGGCAGACCGATATCGTATGCCGTCTTCTG	36512	0.10278503182839628	Illumina Paired End PCR Primer 2 (96% over 26bp)
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	8.44530826810881E-6	2.81510275603627E-6	0.0	5.63020551207254E-6	2.81510275603627E-6
2	4.222654134054405E-5	1.407551378018135E-5	0.0	2.533592480432643E-5	2.81510275603627E-6
3	4.222654134054405E-5	1.689061653621762E-5	0.0	2.533592480432643E-5	2.81510275603627E-6
4	4.222654134054405E-5	2.252082204829016E-5	0.0	3.378123307243524E-5	2.81510275603627E-6
5	6.756246614487048E-5	3.659633582847151E-5	0.0	4.222654134054405E-5	5.63020551207254E-6
6	2.1113270670272024E-4	3.659633582847151E-5	0.0	4.504164409658032E-5	8.44530826810881E-6
7	3.490727417484975E-4	3.659633582847151E-5	0.0	6.193226063279794E-5	8.44530826810881E-6
8	6.840699697168136E-4	3.659633582847151E-5	0.0	6.756246614487048E-5	8.44530826810881E-6
9	6.897001752288861E-4	4.785674685261659E-5	0.0	1.0978900748541453E-4	8.44530826810881E-6
10	7.488173331056478E-4	5.348695236468913E-5	0.0	1.7453637087424875E-4	1.126041102414508E-5
11	8.473459295669173E-4	5.348695236468913E-5	0.0	1.9424209016650264E-4	1.126041102414508E-5
12	8.839422653953888E-4	7.037756890090675E-5	0.0	2.3646863150704667E-4	1.407551378018135E-5
13	0.001033142711465311	7.037756890090675E-5	0.0	2.8432537835966325E-4	1.407551378018135E-5
14	0.004177612489957825	7.600777441297929E-5	0.0	3.7159356379678766E-4	1.407551378018135E-5
15	0.004290216600199275	7.600777441297929E-5	0.0	4.138201051373317E-4	1.407551378018135E-5
16	0.0056076846900242495	8.163797992505183E-5	0.0	4.785674685261659E-4	1.407551378018135E-5
17	0.005624575306560468	8.163797992505183E-5	0.0	5.348695236468912E-4	1.407551378018135E-5
18	0.005658356539632902	9.008328819316064E-5	0.0	5.799111677434716E-4	1.407551378018135E-5
19	0.005714658594753628	9.289839094919692E-5	0.0	6.080621953038343E-4	1.407551378018135E-5
20	0.005748439827826063	9.289839094919692E-5	0.0	6.502887366443784E-4	1.689061653621762E-5
21	0.006015874589649509	9.289839094919692E-5	0.0	7.037756890090675E-4	1.970571929225389E-5
22	0.0060440256172098715	9.852859646126946E-5	0.0	7.572626413737566E-4	1.970571929225389E-5
23	0.0060440256172098715	1.0978900748541453E-4	2.81510275603627E-6	8.079344909824095E-4	2.533592480432643E-5
24	0.006066546439258162	1.1823431575352334E-4	2.81510275603627E-6	8.55791237835026E-4	2.81510275603627E-5
25	0.006122848494378887	1.1823431575352334E-4	2.81510275603627E-6	9.036479846876427E-4	3.378123307243524E-5
26	0.006376207742422152	1.1823431575352334E-4	2.81510275603627E-6	9.79655759100622E-4	3.378123307243524E-5
27	0.006562004524320545	1.2386452126559588E-4	2.81510275603627E-6	0.00139629096699399	3.378123307243524E-5
28	0.006784397642047411	1.407551378018135E-4	2.81510275603627E-6	0.002116957272539275	3.659633582847151E-5
29	0.006792842950315519	1.5201554882595858E-4	2.81510275603627E-6	0.003212032244637384	3.659633582847151E-5
30	0.00681536377236381	1.5483065158199485E-4	2.81510275603627E-6	0.004594247697851193	3.659633582847151E-5
31	0.006823809080631919	1.5483065158199485E-4	2.81510275603627E-6	0.00606091623374609	3.941143858450778E-5
32	0.006826624183387955	1.5483065158199485E-4	2.81510275603627E-6	0.008011782443679224	4.222654134054405E-5
33	0.006832254388900027	1.5483065158199485E-4	2.81510275603627E-6	0.01180372585606008	4.785674685261659E-5
34	0.006832254388900027	1.5483065158199485E-4	2.81510275603627E-6	0.01759439222522669	4.785674685261659E-5
35	0.006832254388900027	1.5764575433803113E-4	2.81510275603627E-6	0.025144497816915964	4.785674685261659E-5
36	0.006832254388900027	1.5764575433803113E-4	2.81510275603627E-6	0.037567546279304025	5.348695236468913E-5
37	0.006832254388900027	1.5764575433803113E-4	5.63020551207254E-6	0.06056130559060827	5.348695236468913E-5
38	0.006832254388900027	1.689061653621762E-4	5.63020551207254E-6	0.09318271632755658	5.348695236468913E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTCTTC	95255	0.0	36.055714	40
GCCGTCT	97755	0.0	35.18698	38
CCGTCTT	97845	0.0	35.172844	39
TTCTGCT	102905	0.0	33.954365	44
TATGCCG	102415	0.0	33.931183	35
ATGCCGT	102885	0.0	33.55966	36
TGCCGTC	103265	0.0	33.363396	37
GTCTTCT	103620	0.0	33.14168	41
CTTCTGC	106270	0.0	32.95953	43
GGTATCA	55480	0.0	31.338009	1
TCTTCTG	110905	0.0	30.938091	42
TATCGTA	79670	0.0	30.705881	30
GACCGAT	113255	0.0	30.700369	24
ACCGATA	81295	0.0	30.677507	25
ATATCGT	80080	0.0	30.583738	29
ATCGTAT	81360	0.0	30.551327	31
TCGTATG	113315	0.0	30.5085	32
CCGATAT	81265	0.0	30.502167	26
CGATATC	81445	0.0	30.468428	27
TGAGCGG	122130	0.0	30.432053	5
>>END_MODULE