FastQCFastQC Report
Thu 26 May 2016
SRR522149_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR522149_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27888611
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCTGAGCGGGCTGGCAAGGCAGACCGATATCGTATGCCGTCTTCTGCT2065640.7406751092766864Illumina PCR Primer Index 1 (95% over 24bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT817540.2931447536056923No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT668410.23967131242212097No Hit
TCTCTGAGCGGGCTGGCAAGGCAGACCGATCTCGTATGCCGTCTTCTGCT587100.21051604183514194Illumina Paired End PCR Primer 2 (96% over 29bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT417620.1497457151953534No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG368850.034.29049335
CGTCTTC371900.034.175840
CCGTCTT371400.034.14480239
GCCGTCT371250.034.0826938
ATGCCGT384700.032.8282736
TATCGTA300800.032.0402630
ATATCGT302050.031.92171729
TGCCGTC396750.031.88663337
GACCGAT396000.031.79096624
ACCGATA305400.031.69431125
ATCGTAT306050.031.67961531
TCGTATG399550.031.60661332
CGATATC306750.031.57951527
GATATCG308550.031.39108828
CGTATGC403600.031.36073533
CCGATAT308750.031.32927526
AGACCGA416150.030.80617323
TGAGCGG429300.030.7845755
GCGGGCT428500.030.7455738
AGCGGGC439500.030.0212087
Produced by FastQC (version 0.11.5)