##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR522149_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 27888611 Sequences flagged as poor quality 0 Sequence length 50 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 39.13793795610689 40.0 39.0 40.0 38.0 40.0 2 39.03419704193945 40.0 39.0 40.0 38.0 40.0 3 38.99589577982209 40.0 39.0 40.0 38.0 40.0 4 39.03479735867806 40.0 39.0 40.0 38.0 40.0 5 39.03505940112973 40.0 39.0 40.0 38.0 40.0 6 39.09544232948711 40.0 39.0 40.0 38.0 40.0 7 39.05885223183041 40.0 39.0 40.0 38.0 40.0 8 39.042080618500506 40.0 39.0 40.0 38.0 40.0 9 39.06434963720495 40.0 39.0 40.0 38.0 40.0 10 39.02670018237911 40.0 39.0 40.0 38.0 40.0 11 39.084001135804144 40.0 39.0 40.0 38.0 40.0 12 39.03074792789071 40.0 39.0 40.0 38.0 40.0 13 39.029998912459284 40.0 39.0 40.0 38.0 40.0 14 39.00478507158352 40.0 39.0 40.0 38.0 40.0 15 38.986231512211205 40.0 39.0 40.0 38.0 40.0 16 38.970026438390924 40.0 39.0 40.0 38.0 40.0 17 38.93757978839462 40.0 39.0 40.0 37.0 40.0 18 38.92070917407826 40.0 39.0 40.0 37.0 40.0 19 38.89407080904818 40.0 39.0 40.0 37.0 40.0 20 38.8672330794818 40.0 39.0 40.0 37.0 40.0 21 38.835561979045856 40.0 39.0 40.0 37.0 40.0 22 38.77844848565603 40.0 39.0 40.0 36.0 40.0 23 38.74924911104393 40.0 39.0 40.0 36.0 40.0 24 38.70150560743237 40.0 39.0 40.0 36.0 40.0 25 38.6678206741813 40.0 39.0 40.0 36.0 40.0 26 38.57767262055468 40.0 39.0 40.0 36.0 40.0 27 38.50577983249148 40.0 38.0 40.0 36.0 40.0 28 38.63613297198631 40.0 39.0 40.0 36.0 40.0 29 38.56262153034441 40.0 39.0 40.0 36.0 40.0 30 38.49479767923903 40.0 39.0 40.0 36.0 40.0 31 38.31451910602504 40.0 39.0 40.0 35.0 40.0 32 38.35249188279761 40.0 39.0 40.0 35.0 40.0 33 38.29983942190595 40.0 38.0 40.0 35.0 40.0 34 38.27205033624658 40.0 38.0 40.0 35.0 40.0 35 38.170416554628694 40.0 38.0 40.0 35.0 40.0 36 38.125112111176854 40.0 38.0 40.0 35.0 40.0 37 38.054484212211214 40.0 38.0 40.0 35.0 40.0 38 37.97940255970439 40.0 38.0 40.0 35.0 40.0 39 38.079865863524006 40.0 38.0 40.0 35.0 40.0 40 38.01748294312686 40.0 38.0 40.0 35.0 40.0 41 38.013367786585 40.0 39.0 40.0 35.0 40.0 42 37.91705961978529 40.0 38.0 40.0 35.0 40.0 43 37.864985602904355 40.0 38.0 40.0 35.0 40.0 44 37.77532075727974 40.0 38.0 40.0 35.0 40.0 45 37.65156884292301 40.0 38.0 40.0 35.0 40.0 46 37.41962692225869 40.0 38.0 40.0 35.0 40.0 47 37.32564436428906 40.0 38.0 40.0 34.0 40.0 48 37.22731311358604 40.0 38.0 40.0 33.0 40.0 49 37.146962966352106 40.0 38.0 40.0 33.0 40.0 50 37.086635974807066 40.0 38.0 40.0 33.0 40.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1 1 -0.02322255953654917 1 2 -0.06867297455379173 1 3 -0.08020414642352591 1 4 -0.07116304909103377 1 5 -0.06916416249131174 1 6 -0.08399437489980244 1 7 -0.0975985482455286 1 8 -0.10267597167921139 1 9 -0.08701635719810241 1 10 -0.09357611472673 1 11 -0.08780184012847059 1 12 -0.10731103469694858 1 13 -0.11705682620900149 1 14 -0.11221913248121496 1 15 -0.12924081493545714 1 16 -0.11326579235930723 1 17 -0.12107659846056151 1 18 -0.1205921257379714 1 19 -0.13112546485833576 1 20 -0.15167647410350327 1 21 -0.15717251830655243 1 22 -0.15486550167376123 1 23 -0.17212322052581186 1 24 -0.17426233558962423 1 25 -0.15838630893937733 1 26 -0.15523533440526904 1 27 -0.1827474793119066 1 28 -0.10302085685763274 1 29 -0.14899117269976614 1 30 -0.15834558501381935 1 31 -0.07260140250530611 1 32 -0.0741526574913749 1 33 -0.08504526940960488 1 34 -0.04595944128399054 1 35 -0.07870156518979599 1 36 -0.05527141529951507 1 37 -0.06329747270289943 1 38 -0.07644607387916125 1 39 -0.08701449265893046 1 40 -0.0769797338997904 1 41 -0.08258985848903677 1 42 -0.06629015629215473 1 43 -0.06239318274221972 1 44 -0.0701297363499549 1 45 -0.07811776539635673 1 46 -0.11092923817965072 1 47 -0.1277113957398015 1 48 -0.11258251309349276 1 49 -0.11167846272010706 1 50 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-0.24910245021053612 119 41 -0.22831965537844212 119 42 -0.24169886851970546 119 43 -0.26073470602255355 119 44 -0.2727663111272989 119 45 -0.258629564245517 119 46 -0.23495583145770382 119 47 -0.2481096757274699 119 48 -0.2868450680252792 119 49 -0.2776153958738732 119 50 -0.28323666190580354 120 1 -0.07032601897292068 120 2 -0.08060595194835685 120 3 -0.07279569509670836 120 4 -0.06451461985430029 120 5 -0.07772193755704393 120 6 -0.06826097102503326 120 7 -0.08256607964616336 120 8 -0.08037186307190325 120 9 -0.04712087995373082 120 10 -0.06638544706797944 120 11 -0.09657422767129731 120 12 -0.10288982751299613 120 13 -0.10675452359770787 120 14 -0.08964911370816964 120 15 -0.1289775715233361 120 16 -0.11666830907676484 120 17 -0.13720682818309626 120 18 -0.12244203644711149 120 19 -0.13984669322771737 120 20 -0.14124222030826417 120 21 -0.15252804211852578 120 22 -0.1641859749968404 120 23 -0.18932395212001296 120 24 -0.1956952320637555 120 25 -0.19160451889130314 120 26 -0.20592348089591894 120 27 -0.21223911155210828 120 28 -0.14917286890070613 120 29 -0.15281573309844276 120 30 -0.13569670806371192 120 31 -0.11105710310501848 120 32 -0.06819060733181459 120 33 -0.08695619000838661 120 34 -0.10177879961694458 120 35 -0.0901332063532081 120 36 -0.10579182392927322 120 37 -0.10554623830561383 120 38 -0.09376891400994936 120 39 -0.11803430113128854 120 40 -0.1171002687424405 120 41 -0.1295143065418074 120 42 -0.11353641816556603 120 43 -0.15612834846296408 120 44 -0.15368691079096664 120 45 -0.2137966607196944 120 46 -0.15835079035384325 120 47 -0.1852839125939596 120 48 -0.155443121808176 120 49 -0.17061267668550073 120 50 -0.17815836720043166 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 8055.0 3 681.0 4 797.0 5 812.0 6 1199.0 7 1753.0 8 2084.0 9 2467.0 10 3604.0 11 4718.0 12 5639.0 13 7281.0 14 8775.0 15 10250.0 16 11813.0 17 13596.0 18 15334.0 19 18020.0 20 22276.0 21 22337.0 22 28227.0 23 41835.0 24 59308.0 25 54799.0 26 83814.0 27 108044.0 28 125508.0 29 128611.0 30 162276.0 31 201520.0 32 252301.0 33 297028.0 34 422313.0 35 620045.0 36 950737.0 37 1697489.0 38 3519780.0 39 1.8469222E7 40 504263.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.33936546660654 18.901877556400223 11.646752210747435 24.1120047662458 2 18.388769231657385 21.01114450326169 35.20390532900404 25.396180936076885 3 18.75281297734558 24.620307222305886 31.554983039664954 25.071896760683575 4 12.462506562281641 15.775088605369078 36.350129678681355 35.41227515366792 5 13.460531361722659 38.17195130543788 35.331382591448424 13.036134741391042 6 32.81628834078542 35.95673517049666 17.338798264280715 13.888178224437208 7 28.354928387608723 32.49562809963881 21.684206261185235 17.465237251567235 8 29.31302544315416 32.762664116329674 19.889967951781877 18.034342488734286 9 26.940015047719655 12.544475592563572 19.768904948331777 40.74660441138499 10 17.156053415496384 26.73364406710682 34.67052912746354 21.439773389933258 11 35.358168250114716 21.248939217517858 22.84958903116401 20.543303501203415 12 21.427797655096004 26.093756213591824 31.281569528776977 21.1968766025352 13 30.23310174761768 20.00624341262955 25.389042559836465 24.3716122799163 14 21.446706636983258 20.313283644325594 28.548669598170633 29.691340120520522 15 25.278872352203408 28.014448645369576 23.55117864432327 23.155500358103748 16 24.07076494415588 26.46478521285983 25.62160948065861 23.84284036232568 17 21.616799058224878 26.936264412738232 27.2720107860517 24.17492574298519 18 22.045635981544866 26.71307223365182 28.063250772501803 23.17804101230151 19 22.557151907530567 26.532653038685606 28.738337227199295 22.17185782658453 20 24.344941533491816 25.290356800137577 28.334467740814652 22.030233925555954 21 24.322838582322408 25.328327831605797 27.828181445170635 22.520652140901166 22 22.983966465737574 25.116098467578755 28.277743914890564 23.62219115179311 23 22.81686249073673 26.305200269230383 27.98797262645167 22.889964613581217 24 23.48324720014549 25.624409435944273 28.256313549282087 22.63602981462815 25 22.90193452763547 26.46100237514309 28.091882326266855 22.545180770954588 26 22.650570852593887 25.383719319021925 28.228676546316706 23.73703328206748 27 22.31854018477734 25.432938648952636 28.273830645096222 23.974690521173798 28 23.459096012366064 25.42118516912928 28.4531008295996 22.66661798890506 29 22.268073955838368 26.500914998553 28.586086069034966 22.644924976573673 30 22.444764001094693 25.89368086804406 29.053505028534904 22.608050102326338 31 22.768154198641437 26.536545501372217 28.181332731564996 22.51396756842135 32 22.212149637939184 25.797989642701157 29.30419921699911 22.68566150236055 33 22.27518623911695 25.795496888015773 28.37206114092362 23.557255731943656 34 23.651535369733114 25.210675434281534 28.467330992584838 22.67045820340051 35 22.39673827953715 25.66434831857442 29.444261814975842 22.494651586912585 36 22.671356195353635 27.129531580048592 27.745056432856348 22.454055791741425 37 22.69579990187104 25.600110749332668 29.052579135352353 22.65151021344394 38 23.727213330266824 26.24662328347627 27.794572209618522 22.231591176638375 39 22.469165829044783 26.069540911461754 28.024281349120084 23.437011910373382 40 22.4668424985865 25.604536997225225 28.312364343937908 23.616256160250366 41 23.425031701215097 25.819353930742654 27.98506804266462 22.770546325377627 42 22.205009144362915 26.01037919179709 29.081714262325608 22.702897401514388 43 22.512694550059006 25.717855034938168 28.006769135508097 23.76268127949473 44 22.5057601252625 26.019040175927643 28.92466709571532 22.55053260309453 45 22.379008227675456 26.030850061612966 28.898568975940726 22.69157273477086 46 22.68910263318534 26.257659453212078 27.439841963476773 23.61339595012581 47 22.33805074595734 26.42238576378374 28.723374267179523 22.516189223079397 48 23.59090068318369 26.363932859455865 27.065810819239733 22.979355638120712 49 22.479891735406234 26.299753648697145 27.456832929227676 23.763521686668945 50 22.510778510934017 26.287674040126678 28.28844555534858 22.91310189359072 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 11539.0 1 9472.0 2 7405.0 3 18131.5 4 28858.0 5 29739.5 6 30621.0 7 24173.0 8 17725.0 9 24252.0 10 30779.0 11 47307.0 12 63835.0 13 104506.0 14 145177.0 15 148646.5 16 152116.0 17 201417.0 18 250718.0 19 245368.0 20 240018.0 21 269573.5 22 299129.0 23 337813.5 24 376498.0 25 418679.0 26 460860.0 27 523758.0 28 586656.0 29 652022.0 30 717388.0 31 793109.5 32 868831.0 33 938583.0 34 1008335.0 35 1086416.5 36 1164498.0 37 1230748.5 38 1296999.0 39 1345975.5 40 1394952.0 41 1435331.0 42 1475710.0 43 1508790.5 44 1541871.0 45 1555088.0 46 1568305.0 47 1568054.5 48 1567804.0 49 1559789.5 50 1551775.0 51 1537378.0 52 1522981.0 53 1523813.5 54 1524646.0 55 1485410.5 56 1446175.0 57 1494898.5 58 1543622.0 59 1402093.5 60 1260565.0 61 1123012.5 62 985460.0 63 876049.0 64 766638.0 65 686307.5 66 605977.0 67 527760.0 68 449543.0 69 389668.5 70 329794.0 71 278202.5 72 226611.0 73 190966.0 74 155321.0 75 123940.0 76 92559.0 77 74740.0 78 56921.0 79 44280.5 80 31640.0 81 23990.0 82 16340.0 83 12464.0 84 8588.0 85 6323.5 86 4059.0 87 3099.0 88 2139.0 89 1247.0 90 355.0 91 246.0 92 137.0 93 94.0 94 51.0 95 35.5 96 20.0 97 22.0 98 24.0 99 33.5 100 43.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1053046349278564 2 5.521967372272502E-4 3 3.944262408766073E-5 4 2.2948435832820789E-4 5 2.725126755147469E-4 6 0.0 7 0.0010075797607847878 8 3.227123788990423E-5 9 0.0 10 0.0 11 0.0 12 0.004152232608501011 13 6.095678268093022E-5 14 2.9402683410801635E-4 15 1.6135618944952115E-4 16 0.0 17 0.0 18 1.0757079296634745E-5 19 0.0010864650089601093 20 0.0012083785743219697 21 3.513979236900683E-4 22 0.0 23 8.175380265442406E-4 24 6.812816887868672E-5 25 0.0036860925056468393 26 0.009333559136380079 27 0.009380173146665498 28 0.016340003451588175 29 0.011908086781374663 30 0.004887299693771052 31 0.022740465633085852 32 0.0036968495849434737 33 0.035623860937355395 34 0.02587436140150544 35 0.03171904115267698 36 0.038058546551493724 37 0.04678612355416338 38 0.06311178423335605 39 0.04328290139655933 40 0.026673970962555287 41 0.02294485013972191 42 0.04257651985608032 43 0.026372772742249515 44 0.035415890737620456 45 0.032005896600587246 46 0.017677466977469764 47 0.01881413168981417 48 0.03014850757536831 49 0.02613611699772355 50 0.02085797675617477 >>END_MODULE >>Sequence Length Distribution pass #Length Count 50 2.7888611E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 35.857619451761366 #Duplication Level Percentage of deduplicated Percentage of total 1 65.08991841095931 23.33969524526375 2 14.618272710483172 10.483529197891476 3 6.905703337868261 7.428662470081153 4 3.737515722286905 5.3607366585895555 5 2.4172750153511013 4.33388638053552 6 1.5440764978486539 3.3220144478559277 7 1.0775583220817821 2.7047073375200936 8 0.761534155093947 2.18454415463019 9 0.5667399002822555 1.8289749305205276 >10 2.930840565940607 18.426171754623038 >50 0.19751648901241364 4.90890777796602 >100 0.14052978494670879 9.59185947819047 >500 0.009458972546893978 2.287201293048013 >1k 0.002878845733312042 1.5931600403015118 >5k 8.056425098484112E-5 0.21795592590050009 >10k+ 1.0070531373071763E-4 1.9879929070822306 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TCTCTGAGCGGGCTGGCAAGGCAGACCGATATCGTATGCCGTCTTCTGCT 206564 0.7406751092766864 Illumina PCR Primer Index 1 (95% over 24bp) GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 81754 0.2931447536056923 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 66841 0.23967131242212097 No Hit TCTCTGAGCGGGCTGGCAAGGCAGACCGATCTCGTATGCCGTCTTCTGCT 58710 0.21051604183514194 Illumina Paired End PCR Primer 2 (96% over 29bp) GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 41762 0.1497457151953534 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 2.8685544791025986E-5 0.0 0.0 0.0 3.5856930988782482E-6 2 7.888524817532145E-5 0.0 0.0 2.8685544791025986E-5 3.5856930988782482E-6 3 8.247094127419971E-5 3.5856930988782482E-6 0.0 4.302831718653898E-5 3.5856930988782482E-6 4 8.247094127419971E-5 3.5856930988782482E-6 0.0 5.378539648317372E-5 3.5856930988782482E-6 5 1.5418480325176466E-4 7.1713861977564964E-6 3.5856930988782482E-6 6.454247577980846E-5 3.5856930988782482E-6 6 5.05582726941833E-4 7.1713861977564964E-6 3.5856930988782482E-6 6.812816887868672E-5 1.0757079296634745E-5 7 7.816810955554581E-4 7.1713861977564964E-6 3.5856930988782482E-6 9.322802057083445E-5 1.0757079296634745E-5 8 0.0014055916947602733 7.1713861977564964E-6 3.5856930988782482E-6 1.0757079296634744E-4 1.0757079296634745E-5 9 0.001445034318847934 7.1713861977564964E-6 3.5856930988782482E-6 1.936274273394254E-4 1.0757079296634745E-5 10 0.001660175904780629 7.1713861977564964E-6 3.5856930988782482E-6 3.9801193397548553E-4 1.0757079296634745E-5 11 0.0018681461045155672 7.1713861977564964E-6 3.5856930988782482E-6 5.05582726941833E-4 1.0757079296634745E-5 12 0.0019721312043830363 7.1713861977564964E-6 3.5856930988782482E-6 7.637526300610668E-4 2.5099851692147737E-5 13 0.0025530134864013127 1.0757079296634745E-5 3.5856930988782482E-6 9.143517402139533E-4 2.5099851692147737E-5 14 0.007626769221314034 1.0757079296634745E-5 3.5856930988782482E-6 0.0013051922879916823 2.5099851692147737E-5 15 0.007924381748520929 1.4342772395512993E-5 3.5856930988782482E-6 0.0018896602631088368 2.5099851692147737E-5 16 0.009831970477124157 1.4342772395512993E-5 3.5856930988782482E-6 0.002237472493700027 2.8685544791025986E-5 17 0.009860656021915182 1.4342772395512993E-5 3.5856930988782482E-6 0.00352115062309844 2.8685544791025986E-5 18 0.009910855725299478 1.792846549439124E-5 3.5856930988782482E-6 0.003933505329469438 2.8685544791025986E-5 19 0.010079383300946756 1.792846549439124E-5 3.5856930988782482E-6 0.005088098507308235 2.8685544791025986E-5 20 0.01021563963870413 1.792846549439124E-5 7.1713861977564964E-6 0.006081335495697509 2.8685544791025986E-5 21 0.010757079296634744 1.792846549439124E-5 7.1713861977564964E-6 0.0071785575839542525 2.8685544791025986E-5 22 0.010825207465513432 1.792846549439124E-5 7.1713861977564964E-6 0.00988575587360733 2.8685544791025986E-5 23 0.0108467216241067 1.792846549439124E-5 7.1713861977564964E-6 0.010810864693117919 3.585693098878248E-5 24 0.01088974994129324 2.5099851692147737E-5 7.1713861977564964E-6 0.014149144968173568 4.302831718653898E-5 25 0.01103317766524837 2.5099851692147737E-5 7.1713861977564964E-6 0.014651142002016521 4.302831718653898E-5 26 0.011710873660936358 2.5099851692147737E-5 7.1713861977564964E-6 0.01627187528270949 4.302831718653898E-5 27 0.012069442970824183 2.5099851692147737E-5 7.1713861977564964E-6 0.01749818232252585 4.302831718653898E-5 28 0.012686182183831243 3.227123788990423E-5 1.0757079296634745E-5 0.01934122857534927 4.302831718653898E-5 29 0.012707696342424512 5.378539648317372E-5 1.0757079296634745E-5 0.02206635533049674 4.302831718653898E-5 30 0.012804510056094225 5.378539648317372E-5 1.0757079296634745E-5 0.0242321139622192 4.302831718653898E-5 31 0.012958694859345988 5.378539648317372E-5 1.0757079296634745E-5 0.02839151795691797 4.661401028541722E-5 32 0.012962280552444867 5.378539648317372E-5 1.0757079296634745E-5 0.03219593833482779 4.661401028541722E-5 33 0.013012480255829163 5.378539648317372E-5 1.0757079296634745E-5 0.03909839755016842 5.378539648317372E-5 34 0.01301606594892804 5.378539648317372E-5 1.0757079296634745E-5 0.04888016832390828 5.378539648317372E-5 35 0.013033994414422432 5.378539648317372E-5 1.0757079296634745E-5 0.06121853827714833 5.378539648317372E-5 36 0.013048337186817945 5.378539648317372E-5 1.0757079296634745E-5 0.08119084883790018 5.378539648317372E-5 37 0.013051922879916824 5.737108958205197E-5 1.0757079296634745E-5 0.11484257857087253 5.378539648317372E-5 38 0.01308060842470785 7.888524817532145E-5 1.0757079296634745E-5 0.1620769137623957 5.378539648317372E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATGCCG 36885 0.0 34.290493 35 CGTCTTC 37190 0.0 34.1758 40 CCGTCTT 37140 0.0 34.144802 39 GCCGTCT 37125 0.0 34.08269 38 ATGCCGT 38470 0.0 32.82827 36 TATCGTA 30080 0.0 32.04026 30 ATATCGT 30205 0.0 31.921717 29 TGCCGTC 39675 0.0 31.886633 37 GACCGAT 39600 0.0 31.790966 24 ACCGATA 30540 0.0 31.694311 25 ATCGTAT 30605 0.0 31.679615 31 TCGTATG 39955 0.0 31.606613 32 CGATATC 30675 0.0 31.579515 27 GATATCG 30855 0.0 31.391088 28 CGTATGC 40360 0.0 31.360735 33 CCGATAT 30875 0.0 31.329275 26 AGACCGA 41615 0.0 30.806173 23 TGAGCGG 42930 0.0 30.784575 5 GCGGGCT 42850 0.0 30.745573 8 AGCGGGC 43950 0.0 30.021208 7 >>END_MODULE