SRR522148_1.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	SRR522148_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	25211221
Sequences flagged as poor quality	0
Sequence length	50
%GC	46

Per base quality graph

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
TCTCTGAGCGGGCTGGCAAGGCAGACCGATATCGTATGCCGTCTTCTGCT	204379	0.8106668058639445	Illumina PCR Primer Index 1 (95% over 24bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	48152	0.19099431955318624	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	41889	0.16615220659086682	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	25371	0.10063376145090315	No Hit
TCTCTGAGCGGGCTGGCAAGGCAGACCGATCTCGTATGCCGTCTTCTGCT	25253	0.10016571589293514	Illumina Paired End PCR Primer 2 (96% over 29bp)

Adapter graph

Kmer graph

Sequence	Count	Obs/Exp Max	Max Obs/Exp Position
TATGCCG	31490	34.50515	35
GCCGTCT	31555	33.957367	38
CCGTCTT	32005	33.507915	39
ATGCCGT	32325	33.243267	36
CGTCTTC	32200	33.18247	40
TGCCGTC	33040	32.456413	37
TATCGTA	28680	32.263325	30
ATATCGT	28765	32.166367	29
GACCGAT	33480	32.099113	24
ACCGATA	28950	32.067802	25
TCGTATG	33790	32.05341	32
ATCGTAT	29310	31.87469	31
CGATATC	29115	31.86653	27
CGTATGC	34235	31.793055	33
GATATCG	29275	31.719904	28
CCGATAT	29245	31.700214	26
AGACCGA	34950	31.513235	23
GCGGGCT	36340	31.404924	8
TGAGCGG	36655	31.350634	5
GGTATCA	25355	30.960623	1