SRR522147_1.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	SRR522147_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	31926854
Sequences flagged as poor quality	0
Sequence length	50
%GC	46

Per base quality graph

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
TCTCTGAGCGGGCTGGCAAGGCAGACCGATATCGTATGCCGTCTTCTGCT	1055483	3.3059411365742455	Illumina PCR Primer Index 1 (95% over 24bp)
TCTCTGAGCGGGCTGGCAAGGCAGACCGATCTCGTATGCCGTCTTCTGCT	311393	0.9753325523397952	Illumina Paired End PCR Primer 2 (96% over 29bp)
CTTATACACATCTCTGAGCGGGCTGGCAAGGCAGACCGATATCGTATGCC	115217	0.360878024499376	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	109543	0.3431061513295359	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	95892	0.30034904159363773	No Hit
CATCTCTGAGCGGGCTGGCAAGGCAGACCGATATCGTATGCCGTCTTCTG	69580	0.21793566005595166	Illumina Paired End PCR Primer 2 (96% over 26bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	58423	0.1829901561863878	No Hit
ATACACATCTCTGAGCGGGCTGGCAAGGCAGACCGATATCGTATGCCGTC	46998	0.1472052335629436	No Hit
ACACATCTCTGAGCGGGCTGGCAAGGCAGACCGATATCGTATGCCGTCTT	41912	0.13127507019639328	No Hit
CTTATACACATCTCTGAGCGGGCTGGCAAGGCAGACCGATCTCGTATGCC	39998	0.12528011685711346	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	31940	0.10004117536917354	No Hit

Adapter graph

Kmer graph

Sequence	Count	Obs/Exp Max	Max Obs/Exp Position
TTCTGCT	176360	37.82943	44
CGTCTTC	175500	37.374947	40
CTTCTGC	180840	36.908634	43
GCCGTCT	180625	36.37456	38
CCGTCTT	180695	36.36444	39
GTCTTCT	183440	35.768623	41
TATGCCG	188825	34.971107	35
ATGCCGT	188775	34.857067	36
TCTTCTG	188370	34.85621	42
TGCCGTC	188735	34.80912	37
GGTATCA	45660	33.58062	1
ACCGATA	150990	32.459858	25
TATCGTA	149540	32.415295	30
TGAGCGG	214920	32.40953	5
ATATCGT	149670	32.377857	29
CCGATAT	150845	32.373367	26
GACCGAT	203620	32.35469	24
CGATATC	151000	32.341946	27
GATATCG	151090	32.333725	28
ATCGTAT	151775	32.28997	31