FastQCFastQC Report
Thu 26 May 2016
SRR522146_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR522146_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26596379
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCTGAGCGGGCTGGCAAGGCAGACCGATATCGTATGCCGTCTTCTGCT1778740.6687902890840892Illumina PCR Primer Index 1 (95% over 24bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT809720.3044474588063285No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT683670.25705378916430693No Hit
TCTCTGAGCGGGCTGGCAAGGCAGACCGATCTCGTATGCCGTCTTCTGCT560030.21056625791052233Illumina Paired End PCR Primer 2 (96% over 29bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT429140.16135279167137753No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG327450.034.01287535
CCGTCTT326900.033.96232639
GCCGTCT329600.033.69269638
CGTCTTC332200.033.5818640
ATGCCGT338500.032.7982936
GACCGAT348100.031.92703424
TGCCGTC348550.031.87958537
TCGTATG349450.031.7261232
ATATCGT256650.031.6577329
TATCGTA257550.031.5139430
ATCGTAT260350.031.37877831
CGTATGC355300.031.327833
ACCGATA260950.031.31029325
GATATCG261400.031.20777528
CGATATC262500.031.0598327
AGACCGA364500.031.03998823
CCGATAT263100.030.99590126
GCGGGCT378050.030.7432618
TGAGCGG380250.030.6691675
CAGACCG379850.029.75645622
Produced by FastQC (version 0.11.5)