SRR522145_1.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	SRR522145_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	31256043
Sequences flagged as poor quality	0
Sequence length	50
%GC	46

Per base quality graph

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
TCTCTGAGCGGGCTGGCAAGGCAGACCGATATCGTATGCCGTCTTCTGCT	492360	1.5752473849616855	Illumina PCR Primer Index 1 (95% over 24bp)
TCTCTGAGCGGGCTGGCAAGGCAGACCGATCTCGTATGCCGTCTTCTGCT	82306	0.2633282786307915	Illumina Paired End PCR Primer 2 (96% over 29bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	60978	0.19509187391379004	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	50538	0.16169033297017157	No Hit
CTTATACACATCTCTGAGCGGGCTGGCAAGGCAGACCGATATCGTATGCC	47640	0.15241852591513264	No Hit
CATCTCTGAGCGGGCTGGCAAGGCAGACCGATATCGTATGCCGTCTTCTG	34104	0.10911170041582038	Illumina Paired End PCR Primer 2 (96% over 26bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	33076	0.10582273642252156	No Hit

Adapter graph

Kmer graph

Sequence	Count	Obs/Exp Max	Max Obs/Exp Position
CGTCTTC	76200	35.888412	40
GCCGTCT	76830	35.713173	38
CCGTCTT	77180	35.550014	39
TATGCCG	78905	35.109592	35
ATGCCGT	79505	34.63371	36
TGCCGTC	80020	34.297302	37
ACCGATA	69040	32.69371	25
TATCGTA	68755	32.68103	30
GACCGAT	83785	32.63134	24
TCGTATG	84575	32.627262	32
ATCGTAT	69780	32.577976	31
ATATCGT	68985	32.548748	29
CGTATGC	85325	32.50115	33
GTCTTCT	84360	32.495453	41
AGACCGA	86255	32.49207	23
CCGATAT	69355	32.434906	26
TGAGCGG	88965	32.427757	5
CGATATC	69505	32.390293	27
GATATCG	69565	32.372425	28
GCGGGCT	88930	32.34918	8