##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR522145_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	31256043
Sequences flagged as poor quality	0
Sequence length	50
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	39.12834011010287	40.0	39.0	40.0	38.0	40.0
2	39.02972730745219	40.0	39.0	40.0	38.0	40.0
3	38.99580724277862	40.0	39.0	40.0	38.0	40.0
4	39.03975615211433	40.0	39.0	40.0	38.0	40.0
5	39.018868159350816	40.0	39.0	40.0	38.0	40.0
6	39.05821344691649	40.0	39.0	40.0	38.0	40.0
7	39.011381927008486	40.0	39.0	40.0	38.0	40.0
8	39.000828031878505	40.0	39.0	40.0	38.0	40.0
9	39.01553763539422	40.0	39.0	40.0	38.0	40.0
10	38.96864715088855	40.0	39.0	40.0	38.0	40.0
11	39.00086488875127	40.0	39.0	40.0	38.0	40.0
12	38.93890160696285	40.0	39.0	40.0	37.0	40.0
13	38.92904952811845	40.0	39.0	40.0	37.0	40.0
14	38.89584196566405	40.0	39.0	40.0	37.0	40.0
15	38.86420728305243	40.0	39.0	40.0	37.0	40.0
16	38.83211880019489	40.0	39.0	40.0	37.0	40.0
17	38.7683423010392	40.0	39.0	40.0	36.0	40.0
18	38.75066885465956	40.0	39.0	40.0	36.0	40.0
19	38.726132639374725	40.0	39.0	40.0	36.0	40.0
20	38.68490822718666	40.0	39.0	40.0	36.0	40.0
21	38.60519842514934	40.0	39.0	40.0	36.0	40.0
22	38.53492603014399	40.0	38.0	40.0	35.0	40.0
23	38.51363190791618	40.0	38.0	40.0	35.0	40.0
24	38.43872450521008	40.0	38.0	40.0	35.0	40.0
25	38.37777949051324	40.0	38.0	40.0	35.0	40.0
26	38.19641680170455	40.0	38.0	40.0	35.0	40.0
27	38.105300053496855	40.0	38.0	40.0	35.0	40.0
28	38.254154980526486	40.0	38.0	40.0	35.0	40.0
29	38.12770692054653	40.0	38.0	40.0	35.0	40.0
30	38.00199465428173	40.0	38.0	40.0	35.0	40.0
31	37.73082021291051	40.0	38.0	40.0	35.0	40.0
32	37.80288451740356	40.0	38.0	40.0	34.0	40.0
33	37.999419760204454	40.0	38.0	40.0	35.0	40.0
34	37.99098926246038	40.0	38.0	40.0	35.0	40.0
35	37.8566452253729	40.0	38.0	40.0	34.0	40.0
36	38.00628953575473	40.0	39.0	40.0	35.0	40.0
37	37.96446850293878	40.0	38.0	40.0	35.0	40.0
38	37.894807957616386	40.0	38.0	40.0	35.0	40.0
39	37.800947739929846	40.0	38.0	40.0	35.0	40.0
40	37.75734465172063	40.0	38.0	40.0	35.0	40.0
41	37.64834710523018	40.0	38.0	40.0	35.0	40.0
42	37.519221067106926	40.0	38.0	40.0	34.0	40.0
43	37.43567495092069	40.0	38.0	40.0	33.0	40.0
44	37.34212878450417	40.0	38.0	40.0	33.0	40.0
45	37.2471136221562	40.0	38.0	40.0	33.0	40.0
46	36.99159468138689	40.0	38.0	40.0	33.0	40.0
47	36.92214056014704	40.0	38.0	40.0	33.0	40.0
48	36.814891379564585	40.0	38.0	40.0	33.0	40.0
49	36.66305056593376	39.0	38.0	40.0	32.0	40.0
50	36.62904360606363	39.0	38.0	40.0	32.0	40.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1	1	-0.1844370710842611
1	2	-0.20298028989362393
1	3	-0.19334512610418386
1	4	-0.1880305512646956
1	5	-0.20527237225697803
1	6	-0.1895305204543618
1	7	-0.22063853721721216
1	8	-0.23230553269910104
1	9	-0.2365798076069865
1	10	-0.23579021239516607
1	11	-0.25145275246389076
1	12	-0.25397393172402616
1	13	-0.26197808388370447
1	14	-0.2556913822477398
1	15	-0.27563654397539494
1	16	-0.2837981874104756
1	17	-0.34097618011173836
1	18	-0.31594414856129305
1	19	-0.33862003128711393
1	20	-0.2963451673782842
1	21	-0.3559846548104417
1	22	-0.37273478552102546
1	23	-0.3753007127456698
1	24	-0.3789913240873233
1	25	-0.38804955370154204
1	26	-0.42464955091431733
1	27	-0.43493323753897073
1	28	-0.3378537423191901
1	29	-0.3420903633230594
1	30	-0.3412798202788352
1	31	-0.28612020740070676
1	32	-0.3469466024923804
1	33	-0.3901831574244454
1	34	-0.4069772261699711
1	35	-0.4177674968186551
1	36	-0.4091542667560759
1	37	-0.4223902776789217
1	38	-0.4605390981997033
1	39	-0.4662993272748608
1	40	-0.5345932963568671
1	41	-0.6606735651050286
1	42	-0.730839816621554
1	43	-0.7276453024606724
1	44	-0.8215159815707196
1	45	-1.0255620862312114
1	46	-3.622446712931776
1	47	-3.9315497171225644
1	48	-2.2016045130802198
1	49	-4.197372537671853
1	50	-4.80751187733777
2	1	-0.1941403859170734
2	2	-0.20372078844339114
2	3	-0.19658219534618127
2	4	-0.2072346382036443
2	5	-0.20449301049575297
2	6	-0.228127920302164
2	7	-0.25995812478844726
2	8	-0.2532203245050155
2	9	-0.2451635706957802
2	10	-0.27286635738748544
2	11	-0.2438596166244622
2	12	-0.24890574441404567
2	13	-0.2825395221213327
2	14	-0.29106661047509164
2	15	-0.31692349661089736
2	16	-0.2752961400562839
2	17	-0.2992378591489029
2	18	-0.3056649222909229
2	19	-0.31874668416024576
2	20	-0.319300207486954
2	21	-0.3324550006478191
2	22	-0.36251574870512826
2	23	-0.38192666339993053
2	24	-0.41932119055449135
2	25	-0.3691588414838378
2	26	-0.4255824874585912
2	27	-0.442806588803748
2	28	-0.3307525960590638
2	29	-0.3145540388255412
2	30	-0.34272090711547776
2	31	-0.2603736332949893
2	32	-0.2828844351166069
2	33	-0.37914997639308723
2	34	-0.36269235038270864
2	35	-0.4052329126154959
2	36	-0.3697774399921059
2	37	-0.41360440165748
2	38	-0.44788861591906937
2	39	-0.43539716678424156
2	40	-0.4774121155312372
2	41	-0.46902970767084184
2	42	-0.5258169097138037
2	43	-0.5293826785624631
2	44	-0.5180885552917829
2	45	-0.517239228127373
2	46	-0.5589486628050224
2	47	-0.5795710942550159
2	48	-0.5887962015127783
2	49	-0.6893467061128362
2	50	-0.6628811928108078
3	1	-0.16568833265241523
3	2	-0.18307466265888195
3	3	-0.19163319609570806
3	4	-0.18939286100009411
3	5	-0.20573821703001016
3	6	-0.21551587180989884
3	7	-0.21667082523816106
3	8	-0.23587649934331978
3	9	-0.2048050220491362
3	10	-0.23784065656455056
3	11	-0.2125363130311726
3	12	-0.21767086244017975
3	13	-0.22097261349824038
3	14	-0.23658923875896676
3	15	-0.23208236112714786
3	16	-0.24385548501978604
3	17	-0.25310619074800655
3	18	-0.24883325865182115
3	19	-0.27715666350501067
3	20	-0.2871894577560852
3	21	-0.2588620662819139
3	22	-0.2936723079755055
3	23	-0.31956809769734207
3	24	-0.3416440424100031
3	25	-0.3557892478058662
3	26	-0.3483529640715233
3	27	-0.3745377376266319
3	28	-0.26484809083677874
3	29	-0.28761632514421365
3	30	-0.30296438442969986
3	31	-0.2603375301806068
3	32	-0.26709172999405695
3	33	-0.30671080746817836
3	34	-0.327611638010481
3	35	-0.3419458668642221
3	36	-0.35026972113563914
3	37	-0.40287710874350324
3	38	-0.4034282018129147
3	39	-0.40768883441149484
3	40	-0.3978750694229305
3	41	-0.37753232161711736
3	42	-0.43146532927834613
3	43	-0.4581126499129624
3	44	-0.4729346986137273
3	45	-0.5001237567962775
3	46	-0.48610338280778365
3	47	-0.5067188857894038
3	48	-0.5353265995965373
3	49	-0.5255251196412871
3	50	-0.47409506169488935
4	1	-0.1479441583240515
4	2	-0.16414534082451837
4	3	-0.16251758558991014
4	4	-0.16667405245141964
4	5	-0.167325439063589
4	6	-0.1786581015870965
4	7	-0.19902925516664283
4	8	-0.2110961332156549
4	9	-0.19636345143613454
4	10	-0.21779450052748928
4	11	-0.21003198593464134
4	12	-0.22222742680315832
4	13	-0.2360513757312077
4	14	-0.21514497522575482
4	15	-0.23140191413156685
4	16	-0.26228310971401925
4	17	-0.2805963468275081
4	18	-0.2867722029577564
4	19	-0.28088561335122364
4	20	-0.3053157280549925
4	21	-0.27853468957871286
4	22	-0.32671650390756923
4	23	-0.32686694712377573
4	24	-0.3339327685591158
4	25	-0.37318644075615737
4	26	-0.33395007306222624
4	27	-0.39683945089797135
4	28	-0.2689727736423393
4	29	-0.24675425413794017
4	30	-0.2970858950130477
4	31	-0.24469704381113644
4	32	-0.28528446545983144
4	33	-0.2830205981987177
4	34	-0.2846952153745832
4	35	-0.2840662006255883
4	36	-0.27158214931139923
4	37	-0.29312328554553346
4	38	-0.3557809361414286
4	39	-0.3836488388752173
4	40	-0.38805251420880893
4	41	-0.3646814801446041
4	42	-0.40168867067284
4	43	-0.4738296942947642
4	44	-0.4983813696304935
4	45	-0.4482351594139047
4	46	-0.4996544207828322
4	47	-0.47692237903041956
4	48	-0.5048700644813024
4	49	-0.5646901034034215
4	50	-0.5866048426004724
5	1	-0.17458139897669156
5	2	-0.19604678532062536
5	3	-0.19253801854958397
5	4	-0.1784931739841511
5	5	-0.20577311479159732
5	6	-0.20571876163008795
5	7	-0.21843526465399776
5	8	-0.23263438344096699
5	9	-0.23874142582915425
5	10	-0.25957456235249765
5	11	-0.23755341568164567
5	12	-0.246142199255047
5	13	-0.2713859530046321
5	14	-0.23812787145801906
5	15	-0.26687002843263485
5	16	-0.2522992281173444
5	17	-0.2626825832929853
5	18	-0.29278909063199876
5	19	-0.2777815924504097
5	20	-0.30090482032107246
5	21	-0.27828805061413675
5	22	-0.2903832734249292
5	23	-0.3177948105730337
5	24	-0.3083828861430362
5	25	-0.34081182770974294
5	26	-0.3037238652653045
5	27	-0.3495792757883862
5	28	-0.22007058686701697
5	29	-0.20642239451242972
5	30	-0.265481132871912
5	31	-0.18952032521008277
5	32	-0.27037847193840747
5	33	-0.286059278148187
5	34	-0.2701747811726065
5	35	-0.3040544558184308
5	36	-0.30357847161985063
5	37	-0.3676227779023762
5	38	-0.36927430876192346
5	39	-0.4147991348664348
5	40	-0.34534461184334475
5	41	-0.37772946295814336
5	42	-0.38187160189504255
5	43	-0.4060783406325541
5	44	-0.4669413256781354
5	45	-0.4289059779731019
5	46	-0.40978540451994405
5	47	-0.3932712691590794
5	48	-0.441072936593649
5	49	-0.4869106318700531
5	50	-0.49251276596841365
6	1	-0.15395061204901594
6	2	-0.1783495453374968
6	3	-0.1542563541852502
6	4	-0.19500982009204648
6	5	-0.16995931484898108
6	6	-0.18494817349020565
6	7	-0.2057648054020973
6	8	-0.19703978321653182
6	9	-0.19524269793750904
6	10	-0.2014149303521222
6	11	-0.2073264182368817
6	12	-0.21124123115035331
6	13	-0.23883563145790276
6	14	-0.22611385488060876
6	15	-0.24220544898113872
6	16	-0.23929216427822553
6	17	-0.2681864292633094
6	18	-0.2791153726457267
6	19	-0.26163931615439395
6	20	-0.31052504133218406
6	21	-0.3118260454812969
6	22	-0.31091541506611264
6	23	-0.2918095702897503
6	24	-0.3479961609905686
6	25	-0.32839233446807015
6	26	-0.3347158028967172
6	27	-0.3580938920425183
6	28	-0.26549029207425434
6	29	-0.3269505519568625
6	30	-0.31778116595806694
6	31	-0.2900088883213314
6	32	-0.3359892408364189
6	33	-0.45364907670688126
6	34	-0.3875327807782796
6	35	-0.37942938566192197
6	36	-0.3635937471955586
6	37	-0.35449983476363656
6	38	-0.3369606768484772
6	39	-0.3379234531829667
6	40	-0.36568359179134546
6	41	-0.37921091388449213
6	42	-0.42535481326943625
6	43	-0.40273100020987584
6	44	-0.4443775340424949
6	45	-0.5485844572319678
6	46	-0.48067245856549334
6	47	-0.5338428151754471
6	48	-0.632901651211462
6	49	-0.6820664558917002
6	50	-0.6072207137165435
7	1	-0.16186256957652034
7	2	-0.1805052793022739
7	3	-0.1819393878114255
7	4	-0.17542328060390844
7	5	-0.1772388591365015
7	6	-0.17404200420015314
7	7	-0.175788449602166
7	8	-0.1937315494042764
7	9	-0.2010677950436417
7	10	-0.18580526206363146
7	11	-0.20763424405084407
7	12	-0.23352948829858633
7	13	-0.25080588507777435
7	14	-0.24916496397503352
7	15	-0.25136380315924356
7	16	-0.2713009648395115
7	17	-0.2563165864379755
7	18	-0.25958658525223655
7	19	-0.2909773802342812
7	20	-0.3009507449703577
7	21	-0.32327867510019104
7	22	-0.330107143123179
7	23	-0.3661699598836279
7	24	-0.3457940346966666
7	25	-0.35100700613748614
7	26	-0.3787337821485437
7	27	-0.45029785178668646
7	28	-0.3509891303594941
7	29	-0.3544658900234836
7	30	-0.46688531737245853
7	31	-0.33332828171302964
7	32	-0.35960698774528765
7	33	-0.36291497529452243
7	34	-0.3830822947391965
7	35	-0.4249884005176341
7	36	-0.38399294683255647
7	37	-0.34315619534261543
7	38	-0.43916909470482324
7	39	-0.45240280097036845
7	40	-0.43733257217692056
7	41	-0.44617005876582994
7	42	-0.5200028702570094
7	43	-0.5805442977766262
7	44	-0.5096987278378649
7	45	-0.6187318831567623
7	46	-0.5960971294977071
7	47	-0.6359321540911154
7	48	-0.6971570130207851
7	49	-0.6425779249651811
7	50	-0.585269992538251
8	1	-0.16462079873377888
8	2	-0.2002424718020137
8	3	-0.19704310468816288
8	4	-0.2023959016818253
8	5	-0.19497958590279296
8	6	-0.19006698751135787
8	7	-0.21160928939716683
8	8	-0.21756978907740177
8	9	-0.20665940124968074
8	10	-0.21404467986319275
8	11	-0.20827404065457955
8	12	-0.22662287453506735
8	13	-0.23345137105346936
8	14	-0.2553437260089666
8	15	-0.31339519604998145
8	16	-0.3605535991234774
8	17	-0.3147056348248398
8	18	-0.28279205330036916
8	19	-0.32168801490131216
8	20	-0.3357161163185367
8	21	-0.3315727385254945
8	22	-0.3157448094291624
8	23	-0.3215752614385039
8	24	-0.32500995675888333
8	25	-0.3437947491660083
8	26	-0.3816627905274075
8	27	-0.3801955788690421
8	28	-0.37982641890435787
8	29	-0.3752565877011804
8	30	-0.401104659851768
8	31	-0.35521433491677357
8	32	-0.3567846855465433
8	33	-0.3340712935407666
8	34	-0.3475786587853804
8	35	-0.3795435563113756
8	36	-0.3379477299716882
8	37	-0.36929313088972293
8	38	-0.3722309920215494
8	39	-0.3543179410595698
8	40	-0.4379718452422878
8	41	-0.39044713957309085
8	42	-0.5141351353183623
8	43	-0.43416354215692365
8	44	-0.5263542753550965
8	45	-0.6123353769860174
8	46	-0.5534614008676186
8	47	-0.6309749454994105
8	48	-0.6005553070951848
8	49	-0.5944180762379645
8	50	-0.5426627640279307
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113	20	-0.1552397220125883
113	21	-0.14010231662043537
113	22	-0.09676684297546245
113	23	-0.10731386717218072
113	24	-0.14606350287613878
113	25	-0.13179267073214618
113	26	-0.12068561837524072
113	27	-0.08907436815995595
113	28	-0.054947361327364774
113	29	-0.10282042134265623
113	30	-0.0985605568980219
113	31	0.022417885939546522
113	32	0.03531075418510454
113	33	0.009697963463786152
113	34	-0.07661995341175043
113	35	0.03351384945147373
113	36	-0.03500636922390754
113	37	-0.0016026245570657238
113	38	0.03285600935208777
113	39	-0.007029844676118557
113	40	-0.003249779242175066
113	41	0.06484866380324661
113	42	0.07303661710574261
113	43	0.0011481032719729
113	44	0.06244783990753433
113	45	0.039394376678977494
113	46	0.10841618563592448
113	47	0.061894185395409806
113	48	0.06822940781001563
113	49	0.12439845300408336
113	50	0.15002895352773749
114	1	-0.05379985786547792
114	2	-0.07138553699324035
114	3	-0.04955441442805153
114	4	-0.07043395706080702
114	5	-0.056543507380197866
114	6	-0.0693347857626847
114	7	-0.07530038694896035
114	8	-0.07671423628553242
114	9	-0.08334804206997148
114	10	-0.10706979425712149
114	11	-0.09079106988620111
114	12	-0.06967260328552527
114	13	-0.08298072348445373
114	14	-0.1139655525275387
114	15	-0.10104299870836542
114	16	-0.07385977930646703
114	17	-0.11564607685413364
114	18	-0.1040625517324898
114	19	-0.10324059890074011
114	20	-0.09739920696748783
114	21	-0.10176175243385899
114	22	-0.09438149662027939
114	23	-0.14286137368878116
114	24	-0.10509626128926897
114	25	-0.15145566145505995
114	26	-0.12086810902139433
114	27	-0.11290859272692444
114	28	-0.10359043758989372
114	29	-0.058667540435784815
114	30	-0.02511081827518069
114	31	-0.019691447353586966
114	32	-0.03198770060832601
114	33	0.024525988026738332
114	34	-0.06179252740522401
114	35	0.02624666536377873
114	36	0.0038524619517517067
114	37	0.006424696265597163
114	38	0.018731495783931962
114	39	0.03665826637344338
114	40	0.04061954476578933
114	41	0.035100169939269676
114	42	0.0670406932013421
114	43	-0.024549390619228006
114	44	-0.02117503281946398
114	45	0.08577645668410838
114	46	0.11305128689836152
114	47	0.11218886991873944
114	48	0.02907372487633353
114	49	0.09582327247048994
114	50	0.14217626384490245
115	1	-0.10371705551708033
115	2	-0.11588100815202296
115	3	-0.1367932456809413
115	4	-0.08444167017426452
115	5	-0.09101133036836018
115	6	-0.10728493624775126
115	7	-0.11742842828296318
115	8	-0.10914993590496636
115	9	-0.08850346406373433
115	10	-0.13078401298081133
115	11	-0.12707389200065933
115	12	-0.1451490603391079
115	13	-0.12215637134789858
115	14	-0.11433546372271763
115	15	-0.13027815276673493
115	16	-0.10866525989807485
115	17	-0.12031584096326498
115	18	-0.1626191966722601
115	19	-0.10999860719493881
115	20	-0.12765128586181618
115	21	-0.1589099358035213
115	22	-0.11110313847954245
115	23	-0.17314451691843402
115	24	-0.14638056453535597
115	25	-0.15446689860323204
115	26	-0.22632931084557129
115	27	-0.19019667155541242
115	28	-0.07863195335686868
115	29	-0.13931024365488298
115	30	-0.05060116693829997
115	31	-0.06117445562053092
115	32	-0.10201147743575234
115	33	-0.04331220574549377
115	34	-0.05739267775278023
115	35	-0.059125740259673876
115	36	-0.039841717197042215
115	37	-0.1020906676798603
115	38	-0.12897440168411833
115	39	-0.12373065395098592
115	40	-0.03400084720938423
115	41	-0.05982654611931082
115	42	-0.033103574661872415
115	43	-0.0824923610057482
115	44	-0.13326094017839551
115	45	-0.1376662115856746
115	46	0.07744389173300448
115	47	0.12761152337022708
115	48	0.10787085151060438
115	49	0.10089277496418703
115	50	-0.016784141743023895
116	1	-0.0917492869555403
116	2	-0.10344634169059219
116	3	-0.12705222588594722
116	4	-0.07859894314089644
116	5	-0.11093917003864817
116	6	-0.12737540475643527
116	7	-0.11020185653432435
116	8	-0.1099351080358204
116	9	-0.1584825989618679
116	10	-0.11988285178147606
116	11	-0.12806213008219203
116	12	-0.1382423783695188
116	13	-0.12363623140703339
116	14	-0.15720649207491988
116	15	-0.1921837701802005
116	16	-0.15090326021962142
116	17	-0.1331337829795558
116	18	-0.19985893101729602
116	19	-0.13941533195463052
116	20	-0.16833939577364987
116	21	-0.1732791147072419
116	22	-0.16100684804217735
116	23	-0.14591914634949887
116	24	-0.15691728960955942
116	25	-0.10860056641963922
116	26	-0.12175792975211408
116	27	-0.1622270590759669
116	28	-0.07481469130843976
116	29	0.019024548991218637
116	30	-0.10080127046914811
116	31	0.054662492400318285
116	32	-0.015588788634310902
116	33	0.027779800858411363
116	34	0.03432045030365316
116	35	-0.022106838390982375
116	36	-0.01908309177483858
116	37	-0.03756799071155115
116	38	-0.04859209631373318
116	39	-0.05197798837798473
116	40	-0.027622157876933784
116	41	0.016458015538709958
116	42	-0.00242470718242771
116	43	0.00566136899300318
116	44	-0.057083889586543535
116	45	0.0687388732131069
116	46	0.014779002745648029
116	47	0.024788407355728737
116	48	0.048968862222977805
116	49	0.09544562304166249
116	50	0.04725047983747288
117	1	-0.08285201709101386
117	2	-0.06896084630518118
117	3	-0.06974512684031708
117	4	-0.06488827668961505
117	5	-0.060534778117840915
117	6	-0.11950225384937596
117	7	-0.06012619510544681
117	8	-0.10814739138827179
117	9	-0.08414437429158284
117	10	-0.10458617987242746
117	11	-0.1058516897364612
117	12	-0.06860899398650844
117	13	-0.07062802843950777
117	14	-0.08596858206708191
117	15	-0.09767076581127299
117	16	-0.100773415731787
117	17	-0.08103374097922256
117	18	-0.11281714694690237
117	19	-0.09929241951904544
117	20	-0.08312084503373995
117	21	-0.08711680166992153
117	22	-0.19091019223898087
117	23	-0.12916659495024163
117	24	-0.13809664665868127
117	25	-0.17230007696296923
117	26	-0.18182576932085226
117	27	-0.16048039086660282
117	28	-0.10631693923095753
117	29	-0.06990332396805599
117	30	-0.10619858273812355
117	31	-0.07972917740244867
117	32	0.03138028214033284
117	33	-0.07812648884855378
117	34	-0.05676045061537138
117	35	-0.01260594321787778
117	36	-0.0031375632238095363
117	37	-0.05056495080427226
117	38	0.006952118520622719
117	39	-0.01934115129034808
117	40	-0.009290548693435596
117	41	0.05104668607456375
117	42	0.008239929901705523
117	43	0.0507682060236192
117	44	0.054159503982560864
117	45	-0.023474032380455867
117	46	0.03596806472728531
117	47	-0.0017764401001159058
117	48	0.06001965583851643
117	49	0.1097176010512726
117	50	0.05635048215581406
118	1	-0.04557586083062404
118	2	-0.04439271483725804
118	3	-0.05288208801182748
118	4	-0.02868225275201297
118	5	-0.050709740892550315
118	6	-0.057665375812355535
118	7	-0.06296689439808745
118	8	-0.08143708087096257
118	9	-0.08021747073881613
118	10	-0.04613388583534572
118	11	-0.08738458101656477
118	12	-0.1045721430326978
118	13	-0.10880475107734355
118	14	-0.09119574092132865
118	15	-0.13548530071216192
118	16	-0.12162174096143019
118	17	-0.10942420492599325
118	18	-0.1359861586012059
118	19	-0.11814925924500841
118	20	-0.15767808800666216
118	21	-0.08480134859574662
118	22	-0.13990232579206463
118	23	-0.12688387322261008
118	24	-0.1546127838765301
118	25	-0.12446966486359656
118	26	-0.12892970355152045
118	27	-0.11218907311665305
118	28	-0.02504897147064611
118	29	-0.01590073711267337
118	30	-0.011797650749436173
118	31	0.04717838073555214
118	32	-0.04639265151553218
118	33	-0.038996712184427906
118	34	-0.024832623328279624
118	35	0.034039777384762715
118	36	-0.002118575448996296
118	37	-0.013827354276628512
118	38	0.03481192072825934
118	39	-0.00975805654695705
118	40	-0.011543623032451933
118	41	-0.07148448460709744
118	42	-0.012116365524541095
118	43	-0.029927231037817137
118	44	0.03672425460042916
118	45	0.04018522549103665
118	46	0.0615634635508826
118	47	-0.026380172364419252
118	48	-0.09003042382234838
118	49	0.047492176653335605
118	50	0.019975625265317376
119	1	-0.06001767103470712
119	2	-0.06940237588030129
119	3	-0.09358075886932227
119	4	-0.05771955563491815
119	5	-0.07797930304290901
119	6	-0.09702491596199536
119	7	-0.09188311143490324
119	8	-0.10432155814276456
119	9	-0.0902215755814808
119	10	-0.08298473832228126
119	11	-0.10534718142569233
119	12	-0.1230834491393864
119	13	-0.13749690952661098
119	14	-0.11812768247816763
119	15	-0.12115961177963896
119	16	-0.15359303989986017
119	17	-0.15027297085751457
119	18	-0.18758526988331425
119	19	-0.17055645626257387
119	20	-0.150037930491294
119	21	-0.16302891176376022
119	22	-0.15280497448220132
119	23	-0.16815357349106108
119	24	-0.2275414957393238
119	25	-0.18208974105463227
119	26	-0.25251828993564374
119	27	-0.23535436414379518
119	28	-0.0481849833184711
119	29	-0.12975383714516653
119	30	-0.12843018807524942
119	31	-0.036094881562398484
119	32	-0.016600622582821245
119	33	-0.03941952036353058
119	34	-0.09802982081541245
119	35	-0.04137488295529579
119	36	-0.0847530653035804
119	37	-0.08465771511314557
119	38	-0.05900146650424176
119	39	-0.05148522000239808
119	40	-0.04805508020645988
119	41	-0.08319208913630405
119	42	-0.07123432281344577
119	43	-0.10970376465610343
119	44	-0.04856485588901194
119	45	-0.06287826391964302
119	46	0.052423503643090896
119	47	-0.01728438219602424
119	48	-0.03844339919129425
119	49	0.018950724886678927
119	50	0.03529058159546139
120	1	-0.08424965225815839
120	2	-0.10179802652356074
120	3	-0.11033899273792258
120	4	-0.10656308165600592
120	5	-0.10882271941230215
120	6	-0.11290971178993203
120	7	-0.12175484505437595
120	8	-0.1218656693544844
120	9	-0.0811336989826188
120	10	-0.14731341386832497
120	11	-0.12849023692852768
120	12	-0.15817737505781793
120	13	-0.1488050522899087
120	14	-0.15464250162415283
120	15	-0.15597888629909562
120	16	-0.16979381738261878
120	17	-0.16315387244350887
120	18	-0.1707293536218657
120	19	-0.17495272625718883
120	20	-0.1647349935729565
120	21	-0.14851564581601195
120	22	-0.17710013597044139
120	23	-0.20153512545622476
120	24	-0.2268055038945178
120	25	-0.21993857554342355
120	26	-0.20748385508471756
120	27	-0.2394210765589193
120	28	-0.09118053921142177
120	29	-0.08777872486765403
120	30	-0.15708868388287556
120	31	-0.08270416383980717
120	32	-0.08167067214498758
120	33	-0.10297187794535034
120	34	-0.079615806123293
120	35	-0.04367756863869943
120	36	-0.06521104390061794
120	37	-0.08597690940209901
120	38	-0.07305564127177888
120	39	-0.08878097614023517
120	40	-0.06928762555376977
120	41	-0.06534340325970334
120	42	-0.09155426879894435
120	43	-0.0929167723198745
120	44	-0.0995374724858209
120	45	-0.11156727342540051
120	46	-0.11277557745157196
120	47	-0.09512664561595585
120	48	-0.09531672316206397
120	49	-0.06993595490204285
120	50	-0.09878689717056943
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	5481.0
3	373.0
4	560.0
5	768.0
6	1218.0
7	2018.0
8	2511.0
9	3097.0
10	4663.0
11	6530.0
12	7887.0
13	10995.0
14	14337.0
15	17486.0
16	21529.0
17	25402.0
18	31330.0
19	41299.0
20	57086.0
21	38861.0
22	43752.0
23	55591.0
24	74296.0
25	69897.0
26	101743.0
27	130969.0
28	155425.0
29	164924.0
30	204582.0
31	256975.0
32	319749.0
33	385967.0
34	541249.0
35	804120.0
36	1256769.0
37	2271569.0
38	4431990.0
39	1.9352085E7
40	340960.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.743844599119164	19.223898993938036	12.584188616972103	24.448067789970697
2	17.67758507456297	20.646128704292614	35.65627467684718	26.020011544297233
3	18.072422042844657	24.488433540972913	32.46716707211298	24.97197734406945
4	12.021224183752242	15.837855738808651	36.71043388313742	35.43048619430169
5	13.307714607380083	37.47968032933663	36.56301918960119	12.64958587368209
6	33.43294287123933	36.336135063545946	17.21041591861132	13.020506146603395
7	27.926346914739014	33.162796711023205	21.880338467668476	17.030517906569298
8	29.451866315899295	33.425977818113445	19.677033334002004	17.445122531985255
9	26.467953732978934	12.760070108682664	19.5169714861219	41.25500467221651
10	17.46032599200097	26.629477698120645	34.740245270330604	21.169951039547776
11	36.118772296288434	21.29183467017882	22.86652856217276	19.722864471359987
12	22.165489726258695	25.448301309286016	31.37787467210741	21.008334292347882
13	29.210073072909452	19.90241375083852	25.596717409174286	25.290795767077746
14	20.999599981354006	19.95012932379188	29.174879878428627	29.875390816425483
15	25.54791724595465	27.943191657370065	23.31708143606022	23.191809660615068
16	25.015428216553197	26.53791460422549	25.164736943828753	23.281920235392562
17	21.68204401305693	26.906025820350965	26.666596920153967	24.745333246438136
18	22.23411005673367	27.902908247214786	27.394475365931637	22.468506330119904
19	22.522521571351454	27.66777995965757	27.720899491593958	22.08879897739702
20	25.177838410319563	25.51052287712811	27.33773753766592	21.973901174886404
21	25.300150758047014	25.489803683722855	26.96853533251154	22.241510225718592
22	23.046001280841217	25.208445438400496	27.272370977738973	24.47318230301931
23	22.693929289691127	27.335796222034954	27.12293455524351	22.847339933030415
24	24.224078085318183	25.688292980426137	27.43457520555158	22.653053728704094
25	22.764301571694144	27.64980449845596	27.237071548187863	22.348822381662032
26	22.624086981538603	25.557751981441594	27.23920877186703	24.578952265152772
27	22.261728447566213	25.611178574433165	27.245930565408894	24.881162412591728
28	24.32612779921821	25.646108154316632	27.486277811916892	22.541486234548266
29	22.124615376775232	27.671779463678263	27.58641180148716	22.617193358059346
30	22.395540689305747	26.030496501239774	29.205470803868156	22.368492005586322
31	22.733188575494882	27.477233604084105	27.251509625703633	22.53806819471738
32	22.065168100616074	25.806919872698764	29.391519167236517	22.73639285944865
33	22.18463634865455	25.83081391727377	27.442562349573556	24.54198738449812
34	24.602069298360128	25.290557451883195	27.63818408678871	22.46918916296797
35	22.347183369048487	25.65143123167386	29.668906256466986	22.332479142810666
36	22.472413615237294	28.140428315136706	26.970350704872782	22.416807364753218
37	22.572727649751183	25.53190574182787	29.43654424365671	22.458822364764234
38	24.67818407413174	26.089316355242946	27.01697755534118	22.215522015284133
39	22.371449838493593	26.06212679896767	27.22428971085011	24.342133651688624
40	22.45165718313707	25.471168222579482	27.628254031476786	24.448920562806663
41	24.26491867350345	25.87549344806059	27.277013279488322	22.582574598947648
42	22.091866056930503	25.822031750439745	29.574846818744316	22.51125537388544
43	22.425675869983237	25.542393322857453	27.353419791181853	24.678511015977453
44	22.342948177532143	25.79980011352213	29.40077818006773	22.456473528877996
45	22.143355082630755	25.780376203171045	29.428654613047406	22.647614101150797
46	22.354215252474763	26.03411225993722	27.130854204805356	24.480818282782664
47	22.14887251894776	25.952864442592105	29.530656958516243	22.36760607994389
48	24.556776154081184	26.029328116845722	26.67845101691453	22.735444712158568
49	22.186563922058316	26.048062433915753	27.194411795659658	24.570961848366277
50	22.342708824744374	25.957271519618153	28.9584599603793	22.741559695258175
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	5873.0
1	5792.5
2	5712.0
3	14953.5
4	24195.0
5	25418.0
6	26641.0
7	21289.0
8	15937.0
9	21845.0
10	27753.0
11	41719.0
12	55685.0
13	89969.0
14	124253.0
15	131702.5
16	139152.0
17	181789.0
18	224426.0
19	229180.0
20	233934.0
21	269328.0
22	304722.0
23	352431.5
24	400141.0
25	452728.0
26	505315.0
27	582168.5
28	659022.0
29	736206.0
30	813390.0
31	904061.0
32	994732.0
33	1076015.5
34	1157299.0
35	1247480.0
36	1337661.0
37	1414273.0
38	1490885.0
39	1549094.0
40	1607303.0
41	1654359.5
42	1701416.0
43	1737634.5
44	1773853.0
45	1781203.0
46	1788553.0
47	1785981.5
48	1783410.0
49	1782910.0
50	1782410.0
51	1751022.0
52	1719634.0
53	1714676.0
54	1709718.0
55	1670037.5
56	1630357.0
57	1814344.0
58	1998331.0
59	1703346.0
60	1408361.0
61	1233729.5
62	1059098.0
63	931527.5
64	803957.0
65	708908.0
66	613859.0
67	530652.5
68	447446.0
69	385139.0
70	322832.0
71	265071.0
72	207310.0
73	176381.5
74	145453.0
75	113733.0
76	82013.0
77	65752.0
78	49491.0
79	38795.5
80	28100.0
81	22367.5
82	16635.0
83	14068.0
84	11501.0
85	9742.0
86	7983.0
87	6588.5
88	5194.0
89	3773.5
90	2353.0
91	1957.5
92	1562.0
93	1135.0
94	708.0
95	506.5
96	305.0
97	212.5
98	120.0
99	84.5
100	49.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.12979890000791208
2	0.002508314952087825
3	0.011684140567633594
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	3.839257579726263E-4
20	0.0
21	0.0
22	7.038638896164815E-5
23	2.015610229356288E-4
24	0.0012317618068288425
25	0.042625357278910835
26	0.06866192243208777
27	0.09605182588211822
28	0.16642541731850063
29	0.1782183368508931
30	0.20519871949241944
31	0.22449098883054389
32	0.24561650366298768
33	0.2522552198945977
34	0.2774951390999814
35	0.28197107356167894
36	0.3240077446783651
37	0.3294306960097284
38	0.3394319620049153
39	0.3496635834548858
40	0.3646686818289826
41	0.38000331647867264
42	0.41385916956922536
43	0.4279108523110235
44	0.5707792249965871
45	0.5281442695737268
46	0.47028665784725215
47	0.45117355386284824
48	0.47027066094066994
49	0.5008183537500253
50	0.5302206680480955
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	3.1256043E7
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.96432670979814
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.63398280587204	42.623496237601024
2	8.295058375131061	8.455041302140533
3	2.867128813781018	4.383638687538354
4	1.380456588537866	2.814161623477487
5	0.8354008140352464	2.1287820010061806
6	0.5829959285926687	1.7827196985167346
7	0.37558250862921516	1.339891677338543
8	0.2856084098490526	1.1644672248490437
9	0.21115267984482758	0.9685128745135078
>10	1.3024200437191105	12.464581942292394
>50	0.1274946710332204	4.530011879070471
>100	0.09151171557188334	8.952840410770044
>500	0.007939637244756168	2.7541141866397423
>1k	0.0031411983937402062	2.6295210138324205
>5k	5.032390574044156E-5	0.1625329699238515
>10k+	7.548585838869708E-5	2.845686270489799
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TCTCTGAGCGGGCTGGCAAGGCAGACCGATATCGTATGCCGTCTTCTGCT	492360	1.5752473849616855	Illumina PCR Primer Index 1 (95% over 24bp)
TCTCTGAGCGGGCTGGCAAGGCAGACCGATCTCGTATGCCGTCTTCTGCT	82306	0.2633282786307915	Illumina Paired End PCR Primer 2 (96% over 29bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	60978	0.19509187391379004	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	50538	0.16169033297017157	No Hit
CTTATACACATCTCTGAGCGGGCTGGCAAGGCAGACCGATATCGTATGCC	47640	0.15241852591513264	No Hit
CATCTCTGAGCGGGCTGGCAAGGCAGACCGATATCGTATGCCGTCTTCTG	34104	0.10911170041582038	Illumina Paired End PCR Primer 2 (96% over 26bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	33076	0.10582273642252156	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	3.1993813164385524E-6
3	0.0	0.0	0.0	1.279752526575421E-5	3.1993813164385524E-6
4	0.0	0.0	0.0	2.8794431847946972E-5	3.1993813164385524E-6
5	3.1993813164385524E-6	1.279752526575421E-5	0.0	4.479133843013973E-5	3.1993813164385524E-6
6	6.398762632877105E-6	1.279752526575421E-5	3.1993813164385524E-6	4.799071974657829E-5	3.1993813164385524E-6
7	6.398762632877105E-6	1.279752526575421E-5	3.1993813164385524E-6	6.078824501233249E-5	3.1993813164385524E-6
8	2.8794431847946972E-5	1.279752526575421E-5	3.1993813164385524E-6	7.038638896164815E-5	6.398762632877105E-6
9	3.519319448082408E-5	1.279752526575421E-5	3.1993813164385524E-6	1.0877896475891077E-4	6.398762632877105E-6
10	4.799071974657829E-5	1.9196287898631313E-5	3.1993813164385524E-6	2.207573108342601E-4	6.398762632877105E-6
11	4.799071974657829E-5	2.559505053150842E-5	6.398762632877105E-6	2.9754246242878535E-4	6.398762632877105E-6
12	5.119010106301684E-5	2.8794431847946972E-5	6.398762632877105E-6	3.423338008589251E-4	6.398762632877105E-6
13	5.7588863695893944E-5	2.8794431847946972E-5	6.398762632877105E-6	3.9032452060550337E-4	6.398762632877105E-6
14	1.5037092187261195E-4	2.8794431847946972E-5	6.398762632877105E-6	4.7990719746578283E-4	9.598143949315657E-6
15	1.5676968450548906E-4	3.519319448082408E-5	6.398762632877105E-6	6.110818314397635E-4	9.598143949315657E-6
16	1.7276659108768182E-4	3.519319448082408E-5	6.398762632877105E-6	7.262595588315514E-4	9.598143949315657E-6
17	1.7276659108768182E-4	3.519319448082408E-5	6.398762632877105E-6	8.478360488562164E-4	9.598143949315657E-6
18	1.7276659108768182E-4	3.839257579726263E-5	6.398762632877105E-6	9.918082080959513E-4	9.598143949315657E-6
19	1.7916535372055893E-4	4.159195711370118E-5	6.398762632877105E-6	0.0011133846981206163	9.598143949315657E-6
20	1.7916535372055893E-4	4.159195711370118E-5	6.398762632877105E-6	0.0012669550013096667	9.598143949315657E-6
21	1.7916535372055893E-4	4.159195711370118E-5	6.398762632877105E-6	0.0014429209737137872	9.598143949315657E-6
22	1.7916535372055893E-4	4.159195711370118E-5	9.598143949315657E-6	0.001586893132953522	9.598143949315657E-6
23	1.7916535372055893E-4	5.119010106301684E-5	9.598143949315657E-6	0.0017212671482439413	9.598143949315657E-6
24	1.8556411635343604E-4	5.438948237945539E-5	9.598143949315657E-6	0.0018588405448507989	1.279752526575421E-5
25	1.8556411635343604E-4	5.7588863695893944E-5	9.598143949315657E-6	0.0020412052798877965	1.279752526575421E-5
26	1.9836164161919024E-4	5.7588863695893944E-5	9.598143949315657E-6	0.002265161972038495	1.279752526575421E-5
27	2.1755792951782155E-4	5.7588863695893944E-5	9.598143949315657E-6	0.002764265457402909	1.599690658219276E-5
28	2.3035545478357577E-4	7.678515159452525E-5	9.598143949315657E-6	0.0036632916073221426	1.599690658219276E-5
29	2.3355483610001433E-4	7.678515159452525E-5	9.598143949315657E-6	0.005090215674453737	1.599690658219276E-5
30	2.3355483610001433E-4	7.678515159452525E-5	9.598143949315657E-6	0.006913863024823712	1.599690658219276E-5
31	2.4315298004932997E-4	7.678515159452525E-5	9.598143949315657E-6	0.009575748280100587	1.599690658219276E-5
32	2.4315298004932997E-4	7.678515159452525E-5	9.598143949315657E-6	0.013315825039017255	1.9196287898631313E-5
33	2.4315298004932997E-4	7.998453291096381E-5	9.598143949315657E-6	0.019468235310528593	1.9196287898631313E-5
34	2.4315298004932997E-4	7.998453291096381E-5	9.598143949315657E-6	0.02871124793371957	2.2395669215069866E-5
35	2.495517426822071E-4	8.638329554384091E-5	9.598143949315657E-6	0.04138399732813267	2.2395669215069866E-5
36	2.495517426822071E-4	8.638329554384091E-5	9.598143949315657E-6	0.060775447487066744	2.559505053150842E-5
37	2.527511239986456E-4	1.0238020212603368E-4	9.598143949315657E-6	0.0934859220663345	2.559505053150842E-5
38	2.527511239986456E-4	1.1197834607534933E-4	9.598143949315657E-6	0.13656239211086316	3.199381316438552E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTCTTC	76200	0.0	35.888412	40
GCCGTCT	76830	0.0	35.713173	38
CCGTCTT	77180	0.0	35.550014	39
TATGCCG	78905	0.0	35.109592	35
ATGCCGT	79505	0.0	34.63371	36
TGCCGTC	80020	0.0	34.297302	37
ACCGATA	69040	0.0	32.69371	25
TATCGTA	68755	0.0	32.68103	30
GACCGAT	83785	0.0	32.63134	24
TCGTATG	84575	0.0	32.627262	32
ATCGTAT	69780	0.0	32.577976	31
ATATCGT	68985	0.0	32.548748	29
CGTATGC	85325	0.0	32.50115	33
GTCTTCT	84360	0.0	32.495453	41
AGACCGA	86255	0.0	32.49207	23
CCGATAT	69355	0.0	32.434906	26
TGAGCGG	88965	0.0	32.427757	5
CGATATC	69505	0.0	32.390293	27
GATATCG	69565	0.0	32.372425	28
GCGGGCT	88930	0.0	32.34918	8
>>END_MODULE