SRR522144_1.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	SRR522144_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	34153493
Sequences flagged as poor quality	0
Sequence length	50
%GC	46

Per base quality graph

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
TCTCTGAGCGGGCTGGCAAGGCAGACCGATATCGTATGCCGTCTTCTGCT	966754	2.830615304853299	Illumina PCR Primer Index 1 (95% over 24bp)
TCTCTGAGCGGGCTGGCAAGGCAGACCGATCTCGTATGCCGTCTTCTGCT	304881	0.8926788249740664	Illumina Paired End PCR Primer 2 (96% over 29bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	110556	0.3237033471217717	No Hit
CTTATACACATCTCTGAGCGGGCTGGCAAGGCAGACCGATATCGTATGCC	98983	0.2898180868352177	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	94345	0.27623821668840726	No Hit
CATCTCTGAGCGGGCTGGCAAGGCAGACCGATATCGTATGCCGTCTTCTG	64729	0.18952380654008066	Illumina Paired End PCR Primer 2 (96% over 26bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	59202	0.17334098155055472	No Hit
ATACACATCTCTGAGCGGGCTGGCAAGGCAGACCGATATCGTATGCCGTC	39533	0.11575097165024965	No Hit
ACACATCTCTGAGCGGGCTGGCAAGGCAGACCGATATCGTATGCCGTCTT	36183	0.10594231166926323	No Hit
CTTATACACATCTCTGAGCGGGCTGGCAAGGCAGACCGATCTCGTATGCC	35867	0.1050170768770269	No Hit

Adapter graph

Kmer graph

Sequence	Count	Obs/Exp Max	Max Obs/Exp Position
TTCTGCT	167130	37.465847	44
CGTCTTC	165680	37.16849	40
CTTCTGC	171520	36.521145	43
GCCGTCT	169965	36.272903	38
CCGTCTT	169985	36.25875	39
GTCTTCT	173840	35.447826	41
TATGCCG	177340	35.06434	35
ATGCCGT	176815	34.969067	36
TGCCGTC	176810	34.87534	37
TCTTCTG	180095	34.236156	42
ACCGATA	137555	32.885654	25
TATCGTA	136340	32.76536	30
CCGATAT	137455	32.7651	26
ATATCGT	136245	32.760822	29
GACCGAT	189575	32.729416	24
TGAGCGG	201680	32.71275	5
ATCGTAT	138415	32.701202	31
CGATATC	137960	32.658657	27
GCGGGCT	201940	32.606506	8
GATATCG	138350	32.601692	28