##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR522144_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	34153493
Sequences flagged as poor quality	0
Sequence length	50
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	38.762627793297746	40.0	39.0	40.0	36.0	40.0
2	38.59651026031217	40.0	39.0	40.0	36.0	40.0
3	38.50850529402659	40.0	38.0	40.0	35.0	40.0
4	38.61118893461351	40.0	39.0	40.0	36.0	40.0
5	38.601695996365585	40.0	39.0	40.0	36.0	40.0
6	38.65298035547931	40.0	39.0	40.0	36.0	40.0
7	38.59689959091446	40.0	39.0	40.0	36.0	40.0
8	38.57233419726644	40.0	39.0	40.0	36.0	40.0
9	38.55750868586121	40.0	39.0	40.0	36.0	40.0
10	38.51588998524983	40.0	38.0	40.0	35.0	40.0
11	38.5789944823506	40.0	39.0	40.0	36.0	40.0
12	38.47921154067609	40.0	38.0	40.0	35.0	40.0
13	38.446229672613576	40.0	38.0	40.0	35.0	40.0
14	38.40032414839677	40.0	38.0	40.0	35.0	40.0
15	38.38202730830489	40.0	38.0	40.0	35.0	40.0
16	38.3746078329382	40.0	38.0	40.0	35.0	40.0
17	38.28812645898327	40.0	38.0	40.0	35.0	40.0
18	38.274341397525575	40.0	38.0	40.0	35.0	40.0
19	38.272296189441	40.0	38.0	40.0	35.0	40.0
20	38.227848876248174	40.0	38.0	40.0	35.0	40.0
21	38.19534145453292	40.0	38.0	40.0	35.0	40.0
22	38.013450630071716	39.0	38.0	40.0	35.0	40.0
23	38.03205616479696	39.0	38.0	40.0	35.0	40.0
24	37.93131832811361	39.0	38.0	40.0	34.0	40.0
25	37.882621259266216	39.0	38.0	40.0	34.0	40.0
26	37.62120038497966	39.0	38.0	40.0	33.0	40.0
27	37.498230678777134	39.0	38.0	40.0	33.0	40.0
28	37.378433620244934	39.0	38.0	40.0	33.0	40.0
29	37.407570347196994	39.0	38.0	40.0	33.0	40.0
30	37.14124757312524	39.0	37.0	40.0	33.0	40.0
31	36.44588484697597	39.0	37.0	40.0	30.0	40.0
32	36.74208585341476	39.0	37.0	40.0	30.0	40.0
33	36.77404926049584	39.0	37.0	40.0	31.0	40.0
34	36.73748623603448	39.0	37.0	40.0	31.0	40.0
35	36.51143635586556	39.0	36.0	40.0	30.0	40.0
36	36.91620833628935	39.0	37.0	40.0	33.0	40.0
37	36.818903764836	39.0	37.0	40.0	32.0	40.0
38	36.71707745969058	39.0	37.0	40.0	32.0	40.0
39	36.569329614396985	39.0	36.0	40.0	31.0	40.0
40	36.53429442780567	39.0	36.0	40.0	31.0	40.0
41	36.50564936359511	39.0	37.0	40.0	32.0	40.0
42	36.31064105214656	39.0	36.0	40.0	31.0	40.0
43	36.19701639302311	39.0	36.0	40.0	31.0	40.0
44	36.243321964169226	39.0	36.0	40.0	31.0	40.0
45	36.00178175040544	39.0	36.0	40.0	30.0	40.0
46	35.66991903287901	39.0	36.0	40.0	30.0	40.0
47	35.60206389431383	39.0	36.0	40.0	30.0	40.0
48	35.418164373406846	39.0	36.0	40.0	29.0	40.0
49	35.15791286706751	39.0	35.0	40.0	28.0	40.0
50	35.14515229818514	39.0	35.0	40.0	29.0	40.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1	1	-0.28431398726132073
1	2	-0.2997586864025479
1	3	-0.2609979323877525
1	4	-0.33937098002819965
1	5	-0.3295855811599182
1	6	-0.2585647606169772
1	7	-0.3125968722691823
1	8	-0.2795345855273368
1	9	-0.3153029336783888
1	10	-0.25611414034266744
1	11	-0.21375517576785796
1	12	-0.24159290270807787
1	13	-0.1912156356361976
1	14	-0.30850603139989374
1	15	-0.1972196741610972
1	16	-0.2753355254299592
1	17	-0.24963812814703346
1	18	-0.259754777179495
1	19	-0.4921923943418065
1	20	-0.2860995894869802
1	21	-0.33894107047169086
1	22	-0.30587895861553704
1	23	-0.32756539384678973
1	24	-0.33230636837611627
1	25	-0.31848743183756767
1	26	-0.35072288314003686
1	27	-0.339549736490774
1	28	-0.26469180877150933
1	29	-0.20943336508739208
1	30	-0.12945574095918744
1	31	-0.0808504642749881
1	32	-0.26257904178443425
1	33	-0.21260149178816334
1	34	-0.0977567558083976
1	35	-0.21912817012505315
1	36	-0.09949306943045144
1	37	-0.17313603333158056
1	38	-0.24577719047503876
1	39	-0.14357144339106753
1	40	-0.15210854057547607
1	41	-0.2061349976518585
1	42	-0.13158217822520868
1	43	-0.1309226348220207
1	44	-0.17538076515616297
1	45	-0.27965847404349375
1	46	-0.6865788087635849
1	47	-0.553309399724526
1	48	-0.4028212212429878
1	49	-0.5188496703759498
1	50	-0.33911988917945024
2	1	-0.24003611037910133
2	2	-0.2692953883790068
2	3	-0.23218179665639838
2	4	-0.2158027642053426
2	5	-0.21849440411900645
2	6	-0.24344853787722798
2	7	-0.2633706159288778
2	8	-0.24199584553008435
2	9	-0.2463142881128988
2	10	-0.2684296231873091
2	11	-0.23182323688972417
2	12	-0.2531153481231243
2	13	-0.29786709425400915
2	14	-0.24677087926274055
2	15	-0.23618186305495925
2	16	-0.31453415791467876
2	17	-0.2590821797866951
2	18	-0.3242126740617195
2	19	-0.2974542530000832
2	20	-0.2848188529777147
2	21	-0.37977799542598234
2	22	-0.4015667518212922
2	23	-0.41932042345876397
2	24	-0.35173294282317613
2	25	-0.3926933014199534
2	26	-0.3881726877551088
2	27	-0.43618425644833536
2	28	-0.4792980572719685
2	29	-0.5620212535199371
2	30	-0.4364124613217868
2	31	-0.13712334531172843
2	32	-0.29425621338278063
2	33	-0.23520887026543846
2	34	-0.3989316693578786
2	35	-0.3615804011095136
2	36	-0.38572910587339493
2	37	-0.3733438531343438
2	38	-0.3386279702108368
2	39	-0.3665317256184508
2	40	-0.5623590394990003
2	41	-0.3356101784257106
2	42	-0.3879889965008658
2	43	-0.3275775358909243
2	44	-0.5229455135249097
2	45	-0.5532884381364198
2	46	-0.6400653639081071
2	47	-0.6763487350812056
2	48	-0.641981320206753
2	49	-0.6499242349778669
2	50	-0.7447797951735282
3	1	-0.19714161887043247
3	2	-0.26976535645916044
3	3	-0.18659527387749364
3	4	-0.18834309401405136
3	5	-0.20680132902236892
3	6	-0.16927270578100462
3	7	-0.190775853437394
3	8	-0.24116312053966737
3	9	-0.19002449051029657
3	10	-0.1992955846338731
3	11	-0.2092283246525426
3	12	-0.1707743956240506
3	13	-0.1820271391987447
3	14	-0.27718253057976483
3	15	-0.21540606158981035
3	16	-0.2975350828801311
3	17	-0.19669571819469667
3	18	-0.15709146802517893
3	19	-0.24378488459142034
3	20	-0.22377293997651293
3	21	-0.23154662453505637
3	22	-0.3115707200100175
3	23	-0.22790152150314213
3	24	-0.3100280234444597
3	25	-0.2717543456237834
3	26	-0.258382011362265
3	27	-0.39276336612058316
3	28	-0.3603560831329631
3	29	-0.2697581663256301
3	30	-0.18522325885393087
3	31	-0.07111296831566705
3	32	-0.30114443073467356
3	33	-0.21034093114356267
3	34	-0.11667765038703237
3	35	-0.1541070826947788
3	36	-0.27887034911930186
3	37	-0.15301542912740018
3	38	-0.1523577951787587
3	39	-0.19695863797557678
3	40	-0.18536765883742135
3	41	-0.24114829866788767
3	42	-0.26762222650226875
3	43	-0.2693436146932626
3	44	-0.2713425046076239
3	45	-0.3250840837812632
3	46	-0.5541328883390406
3	47	-0.47385165004644847
3	48	-0.4946058301518903
3	49	-0.38783196557385935
3	50	-0.4547211846098165
4	1	-0.24858361846467858
4	2	-0.35381230506453676
4	3	-0.3753939126173549
4	4	-0.32261522824786937
4	5	-0.3288964954909659
4	6	-0.24569305701712807
4	7	-0.2333834613368424
4	8	-0.23551178529724126
4	9	-0.22466507753701848
4	10	-0.23937964670381717
4	11	-0.22530946764246096
4	12	-0.17980989207135423
4	13	-0.24685920615211643
4	14	-0.24664176386284709
4	15	-0.22206571052684154
4	16	-0.21196517730169262
4	17	-0.28067592048500245
4	18	-0.23304500743804368
4	19	-0.226477053914671
4	20	-0.2064544254723657
4	21	-0.2460391059417688
4	22	-0.23063163650064666
4	23	-0.294659846477181
4	24	-0.21965908194927408
4	25	-0.2509712045607415
4	26	-0.3689091354579901
4	27	-0.2761691101617032
4	28	-0.24865179924066183
4	29	-0.29760199122534914
4	30	-0.24349474535446092
4	31	-0.08317005206505712
4	32	-0.182157936536818
4	33	-0.28128786076273826
4	34	-0.13363413529387458
4	35	-0.3214345205840772
4	36	-0.1594533698703131
4	37	-0.12160361728209779
4	38	-0.13192324708750647
4	39	-0.26625461870172984
4	40	-0.22892845411014662
4	41	-0.2813868467516727
4	42	-0.2922086342210264
4	43	-0.23506662079844887
4	44	-0.219334251412036
4	45	-0.23601550958674267
4	46	-0.3630576122684275
4	47	-0.36419441406351183
4	48	-0.5045552780487625
4	49	-0.31531049797500543
4	50	-0.3971230333096827
5	1	-0.4973218723181816
5	2	-0.7816859378843617
5	3	-0.7996542215365565
5	4	-0.7854751344345203
5	5	-0.7335304572689765
5	6	-0.6264531652712719
5	7	-0.5702690684947385
5	8	-0.5589884625225068
5	9	-0.48075667474181927
5	10	-0.56853090243807
5	11	-0.526534398482795
5	12	-0.44480463350324584
5	13	-0.39476400904454323
5	14	-0.44430815520778566
5	15	-0.4491525440687667
5	16	-0.48878175166586857
5	17	-0.4007604245037584
5	18	-0.43797444276042796
5	19	-0.4695203193674189
5	20	-0.4798523849368763
5	21	-0.5421546609822698
5	22	-0.5021658731892629
5	23	-0.4986091228576015
5	24	-0.5406532682164951
5	25	-0.5582193648369156
5	26	-0.6277321462137877
5	27	-0.6228551959570297
5	28	-0.6224023151345932
5	29	-0.5703465042372642
5	30	-0.6171544211246527
5	31	-0.47517699652731693
5	32	-0.5773809372397523
5	33	-0.67075675563283
5	34	-0.5951676698665977
5	35	-0.6706387731645265
5	36	-0.5137476542412713
5	37	-0.524553344249675
5	38	-0.6431170683333249
5	39	-0.666659326845803
5	40	-0.7238521148094321
5	41	-0.7599710821153494
5	42	-0.7691811909146224
5	43	-0.8414504652034722
5	44	-0.8737803221275868
5	45	-0.8479366913519186
5	46	-1.0970864385787706
5	47	-0.9351092965298449
5	48	-1.0855554796411013
5	49	-1.0936454413959922
5	50	-1.1453018160123563
6	1	-0.21722993882093533
6	2	-0.34275239706022376
6	3	-0.31223172769998087
6	4	-0.3395547539520152
6	5	-0.32920002964638684
6	6	-0.27389824721030465
6	7	-0.3075997596437432
6	8	-0.28426490989631503
6	9	-0.2091005793697036
6	10	-0.32388948442866905
6	11	-0.22069848930507874
6	12	-0.2149052545712351
6	13	-0.2037062689474638
6	14	-0.2049210159451249
6	15	-0.23843624596628388
6	16	-0.2824962058494336
6	17	-0.25553221711618335
6	18	-0.24850362182856145
6	19	-0.31380321542128087
6	20	-0.2737103290022844
6	21	-0.275490964415134
6	22	-0.25152495020346066
6	23	-0.3492152610516257
6	24	-0.333240944920945
6	25	-0.3555513836666009
6	26	-0.3871871794484676
6	27	-0.3372338510495041
6	28	-0.3216478386512378
6	29	-0.21113010646397612
6	30	-0.19617772210232687
6	31	-0.0857384962061829
6	32	-0.17520354091381307
6	33	-0.16783380101887957
6	34	-0.22990314090060338
6	35	-0.3683243488677945
6	36	-0.32065712074710007
6	37	-0.2861963361091213
6	38	-0.3975694665999896
6	39	-0.29365739971093063
6	40	-0.23198024617460078
6	41	-0.297918723886788
6	42	-0.2170792889068096
6	43	-0.3639789096842776
6	44	-0.29695932619642207
6	45	-0.28366870191529614
6	46	-0.30801231841765286
6	47	-0.40342488159364365
6	48	-0.38515614806787823
6	49	-0.5505891112953236
6	50	-0.4329030559585618
7	1	-0.15953520494942097
7	2	-0.17328272329013572
7	3	-0.12236616915790677
7	4	-0.16268066025961048
7	5	-0.15866546754514133
7	6	-0.14128122729827197
7	7	-0.14350558148275638
7	8	-0.1472350880008122
7	9	-0.15882101234630142
7	10	-0.1572059027252095
7	11	-0.16670632974861377
7	12	-0.1169617151567266
7	13	-0.0852996784933211
7	14	-0.16272166759316775
7	15	-0.2173592374282265
7	16	-0.13861401568473752
7	17	-0.12811218363050614
7	18	-0.15560426225086843
7	19	-0.17969432882870962
7	20	-0.17612432672860479
7	21	-0.18725959584403284
7	22	-0.1592937288595948
7	23	-0.19206731719973646
7	24	-0.19541224734928164
7	25	-0.1656684168810898
7	26	-0.18630500258218774
7	27	-0.10795956995525557
7	28	-0.11396022148925766
7	29	-0.12419160679246488
7	30	-0.1442217880669432
7	31	-0.08732846390977045
7	32	-0.08074197114128623
7	33	-0.09693276269224782
7	34	-0.16006969984098163
7	35	-0.1685446947587721
7	36	-0.09392605805967236
7	37	-0.20913663307679542
7	38	-0.18751810913285993
7	39	-0.11213479835674178
7	40	-0.14399955913501827
7	41	-0.11704609151790635
7	42	-0.17426665120677853
7	43	-0.08171609544681502
7	44	-0.13176374155352732
7	45	-0.13371778896313913
7	46	-0.15043482050690216
7	47	-0.1983164680838172
7	48	-0.12497936848617286
7	49	-0.2135808212007433
7	50	-0.2398702669587749
8	1	-0.1630184199206539
8	2	-0.16380526490343073
8	3	-0.16542772188405763
8	4	-0.13311078294151457
8	5	-0.16530501920885143
8	6	-0.1736440943584654
8	7	-0.18937047333472634
8	8	-0.21315358654663896
8	9	-0.14867307286353793
8	10	-0.2342742602490091
8	11	-0.13079933139209032
8	12	-0.1089612686992325
8	13	-0.14079358615610715
8	14	-0.11993737264448612
8	15	-0.09084848962191217
8	16	-0.11805675946877159
8	17	-0.11463531529797422
8	18	-0.20389926806602432
8	19	-0.13583443955580776
8	20	-0.15487834893505692
8	21	-0.1777951458869822
8	22	-0.19719470438283082
8	23	-0.18241770095291798
8	24	-0.17341001066964168
8	25	-0.25184916504107235
8	26	-0.1534986074792002
8	27	-0.1251906915731169
8	28	-0.2285822151243906
8	29	-0.11148393612424456
8	30	-0.2587699634730498
8	31	-0.019191571275605668
8	32	-0.10277420710342966
8	33	-0.15628212717453493
8	34	-0.08827865532414592
8	35	-0.043713447892884005
8	36	-0.024517412176315645
8	37	-0.05037796765990521
8	38	-0.01390754954275053
8	39	-0.009110338051293354
8	40	-0.23795742667096675
8	41	-0.2459909520991701
8	42	-0.23544510332771296
8	43	-0.06048376523725807
8	44	-0.18400564832726474
8	45	-0.10297125308952104
8	46	-0.1427876133475081
8	47	-0.21759301469555936
8	48	-0.1531514562359675
8	49	-0.10447975670721377
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113	45	0.07327143481087717
113	46	0.10638543932491729
113	47	0.19334107835103964
113	48	0.2603195716122215
113	49	0.1952270212453513
113	50	0.18019727684605158
114	1	-0.19106045632052826
114	2	-0.1726128707002772
114	3	-0.18720690073791957
114	4	-0.1796944607806381
114	5	-0.11889192147071981
114	6	-0.17869441153413845
114	7	-0.23777160574085343
114	8	-0.3399271686850085
114	9	-0.3520523594613536
114	10	-0.1992945170502125
114	11	-0.4186416090139815
114	12	-0.32901094378283346
114	13	-0.4898343592788663
114	14	-0.5433705202034602
114	15	-0.45923939352417875
114	16	-0.3847008627171391
114	17	-0.37095552993790193
114	18	-0.4132237856254406
114	19	-0.3104146852843428
114	20	-0.43081969892337924
114	21	-0.1925719279386584
114	22	-0.23395988292984526
114	23	-0.1716072584606536
114	24	-0.1692054593037895
114	25	-0.25303539781136664
114	26	-0.22350085833879518
114	27	-0.40847317018739204
114	28	-0.1958975302424193
114	29	-0.21528178730166303
114	30	-0.34270514562874155
114	31	-0.4947310979582795
114	32	-0.12474682066510923
114	33	-0.10878173605215125
114	34	-0.061973256983065994
114	35	-0.053039362506723364
114	36	2.3269784578872077E-4
114	37	-0.029474531720026675
114	38	-0.19961208608015113
114	39	0.0018838805260372737
114	40	0.004452152213971772
114	41	-0.023094882311703202
114	42	0.0926415723356726
114	43	0.14950937154992516
114	44	0.09372813549567383
114	45	-0.04970094385201662
114	46	0.11699970125847159
114	47	0.04965648151303981
114	48	0.16319981590772414
114	49	0.055659497091212984
114	50	0.18835437585151737
115	1	-0.28949807079620626
115	2	-0.38841133873386013
115	3	-0.3300762919667619
115	4	-0.3505761736774673
115	5	-0.35156812163319984
115	6	-0.3764840977076176
115	7	-0.380939869640315
115	8	-0.4079107401497808
115	9	-0.29480075165347586
115	10	-0.28590689441455197
115	11	-0.3051216012441671
115	12	-0.429384380130891
115	13	-0.3748141662828104
115	14	-0.4349087738307347
115	15	-0.30768571380598786
115	16	-0.2872068267207908
115	17	-0.2879694769529664
115	18	-0.2733007507487528
115	19	-0.3478674512260014
115	20	-0.4421004506754329
115	21	-0.2830219532724314
115	22	-0.35545387598207867
115	23	-0.3427281210237467
115	24	-0.35213711775995904
115	25	-0.4069430248446224
115	26	-0.2736808246264104
115	27	-0.38690029605568554
115	28	-0.3136593528022118
115	29	-0.18934580324528127
115	30	-0.20124992851101808
115	31	-0.08156022985320988
115	32	0.009261824320034862
115	33	-0.15705780613327391
115	34	-0.09530755653263157
115	35	-0.06863801374628054
115	36	-0.21719950670654242
115	37	-0.1536420190965586
115	38	-0.24882581565365314
115	39	-0.024150134955462477
115	40	-0.2500141646571805
115	41	-0.10262317173421565
115	42	-0.09295723039150516
115	43	-0.025750384004595617
115	44	-0.0512107448400414
115	45	0.02511509667473888
115	46	0.05533588160007952
115	47	0.015568450060285954
115	48	0.01937356539208679
115	49	0.10443331205664208
115	50	0.09436312656386292
116	1	-0.5132238072996387
116	2	-0.7289460790454356
116	3	-0.7408158862366534
116	4	-0.6982971216694693
116	5	-0.6898814775533921
116	6	-0.6129559198673178
116	7	-0.6577680737472846
116	8	-0.6012321216573113
116	9	-0.5054297676203134
116	10	-0.5758286301573676
116	11	-0.510886208003086
116	12	-0.513151989001301
116	13	-0.4835150603977425
116	14	-0.42415584211651947
116	15	-0.5594771458225409
116	16	-0.524652370772408
116	17	-0.5193958106677314
116	18	-0.492975180957373
116	19	-0.5100336285546376
116	20	-0.4215839381399036
116	21	-0.5232471164540939
116	22	-0.504276380235261
116	23	-0.5718240970434749
116	24	-0.6605633235451265
116	25	-0.5112864539977124
116	26	-0.6459661915676307
116	27	-0.47939035755857873
116	28	-0.5746162130257488
116	29	-0.3844951004070083
116	30	-0.3251198883011952
116	31	-0.09287097628092056
116	32	-0.43780210772419537
116	33	-0.2657561268682045
116	34	-0.30822053967294494
116	35	-0.3095933403553488
116	36	-0.3107183230762445
116	37	-0.2593090869143566
116	38	-0.1983502357707323
116	39	-0.17388134817286272
116	40	-0.351058847768698
116	41	-0.38945147051137496
116	42	-0.38413120052062055
116	43	-0.2695352449925963
116	44	-0.3826960845419407
116	45	-0.49714559359434674
116	46	-0.4850004765667535
116	47	-0.34756482205350636
116	48	-0.4626321064225962
116	49	-0.22410041208049591
116	50	-0.41592887591945527
117	1	-0.2786082396833578
117	2	-0.3850920669108078
117	3	-0.3260774425327
117	4	-0.3806167882049394
117	5	-0.35417414755757903
117	6	-0.3699105127896445
117	7	-0.4137436167389339
117	8	-0.4715449001146155
117	9	-0.49331818382100323
117	10	-0.4386569564336469
117	11	-0.43783187807582635
117	12	-0.5059053073787894
117	13	-0.409812948216441
117	14	-0.6630653798382511
117	15	-0.6051636699725407
117	16	-0.44030700479520846
117	17	-0.43335871692402606
117	18	-0.35518359663992527
117	19	-0.33790286991351337
117	20	-0.4197788567860812
117	21	-0.4206693860409132
117	22	-0.3853185532246073
117	23	-0.39992738659316274
117	24	-0.2654549510927424
117	25	-0.4514868531369487
117	26	-0.24981529494029076
117	27	-0.38530976693798635
117	28	-0.393630707994717
117	29	-0.22846632678894707
117	30	-0.18097840758158412
117	31	-0.24966742197216973
117	32	-0.12023201819099683
117	33	-0.14402721030507593
117	34	-0.0942519123410932
117	35	-0.2914344859589164
117	36	-0.2993503753803566
117	37	-0.1585720980607519
117	38	-0.27669185673102703
117	39	0.02645476050251716
117	40	-0.050868859888325346
117	41	0.01853055313396368
117	42	0.08997582536428439
117	43	-0.05578112822293946
117	44	0.04557995360067224
117	45	0.06096960515027661
117	46	-0.11120267456610833
117	47	-0.20531945743464064
117	48	-0.1719333513944008
117	49	-0.03684797450203092
117	50	-0.16908638488548178
118	1	-0.2848206751052942
118	2	-0.31764879383167965
118	3	-0.2345454696496745
118	4	-0.30315493018146356
118	5	-0.275102904150863
118	6	-0.33769719621489713
118	7	-0.339066011305313
118	8	-0.5320675475824075
118	9	-0.36825147691138227
118	10	-0.4264042986302172
118	11	-0.5068929782218277
118	12	-0.4130398919988423
118	13	-0.4740656670948127
118	14	-0.5412766259305997
118	15	-0.44850680786002783
118	16	-0.4908618367375581
118	17	-0.2731217938415611
118	18	-0.47348331504083063
118	19	-0.36590320516372543
118	20	-0.4590815200634637
118	21	-0.40774938490439894
118	22	-0.3976927094969511
118	23	-0.4032599059915398
118	24	-0.3692025982945779
118	25	-0.5644477546300095
118	26	-0.5038050349643797
118	27	-0.44428325047135786
118	28	-0.3201991298988176
118	29	-0.41591652249128686
118	30	-0.492753567847906
118	31	-0.37353301480356293
118	32	-0.36106766754588904
118	33	-0.4708001006986038
118	34	-0.18459542741682355
118	35	-0.2888846721928644
118	36	-0.13646105759402616
118	37	-0.2801232089035608
118	38	-0.15602360474061072
118	39	-0.05263993352222229
118	40	-0.10468770686530604
118	41	-0.09211739824790754
118	42	-0.19814426994343393
118	43	-0.11642529147987091
118	44	-0.2030213682494022
118	45	-0.014427031007087976
118	46	-0.01227492369266514
118	47	-0.18400760390133541
118	48	-0.14937116067486755
118	49	-0.09299606414545991
118	50	0.030198595176784693
119	1	-0.3909136055388416
119	2	-0.38312612309965033
119	3	-0.39500166141417736
119	4	-0.4227187190927779
119	5	-0.3945613089727402
119	6	-0.560818316774629
119	7	-0.41291779668201656
119	8	-0.7023401516666965
119	9	-0.4088530174080276
119	10	-0.4048303838924241
119	11	-0.36715974985212796
119	12	-0.4924732692839626
119	13	-0.40776787963753236
119	14	-0.3903660112201095
119	15	-0.4592018616214091
119	16	-0.4625174233644742
119	17	-0.5653531051684269
119	18	-0.44700708680040435
119	19	-0.461726771708463
119	20	-0.4532560510160337
119	21	-0.47324805484058885
119	22	-0.3983390576799337
119	23	-0.5079771860843962
119	24	-0.558351548277237
119	25	-0.5363294297772541
119	26	-0.45814436308490514
119	27	-0.5103370302830186
119	28	-0.4265803295792594
119	29	-0.27058540023502786
119	30	-0.1828429399675997
119	31	-0.14131734520486106
119	32	-0.39165027783145234
119	33	0.012203325253175024
119	34	-0.20008404579046868
119	35	-0.13840198015285665
119	36	-0.2942291564340138
119	37	-0.24523142631993267
119	38	-0.08814776820953796
119	39	-0.18498174684044244
119	40	-0.1571964096561942
119	41	-0.18848386095146452
119	42	-0.07011850889142579
119	43	-0.14626032868200411
119	44	-0.14860124575487532
119	45	-0.14883973459789956
119	46	-0.09684065791852703
119	47	-0.12329508807152223
119	48	-0.14563496630975692
119	49	-0.03403830901670801
119	50	-0.1239491254052254
120	1	-0.2563666050792648
120	2	-0.2653409857623288
120	3	-0.2953854181284967
120	4	-0.38738661917475525
120	5	-0.24197727254895085
120	6	-0.32620137656721937
120	7	-0.28670961951650753
120	8	-0.39845262402398873
120	9	-0.43603589470532
120	10	-0.5031127462103555
120	11	-0.4878861444857705
120	12	-0.38179021418703485
120	13	-0.5245551851483015
120	14	-0.5559134933768064
120	15	-0.4620039656400934
120	16	-0.38881236707968725
120	17	-0.3849520380570439
120	18	-0.5763355917741322
120	19	-0.37576660460239
120	20	-0.5853238008864068
120	21	-0.45407535416859446
120	22	-0.6749912882874938
120	23	-0.4187149551079443
120	24	-0.7080207188281449
120	25	-0.4851037759272572
120	26	-0.4506478619291059
120	27	-0.43737246056266343
120	28	-0.7162953574978062
120	29	-0.28597692294341215
120	30	-0.5282540136207174
120	31	-0.3133813055375114
120	32	-0.49599413447667473
120	33	-0.2944850437791402
120	34	-0.3205615787089897
120	35	-0.39216248153378075
120	36	-0.431797364193109
120	37	-0.30160368832321893
120	38	-0.4349110845931108
120	39	-0.4469749198958084
120	40	-0.31865709984555934
120	41	-0.3852646519563976
120	42	-0.3003040749284551
120	43	-0.45290925912376423
120	44	-0.450088284454786
120	45	-0.3435612188423107
120	46	-0.3651491137454457
120	47	-0.6404465836721656
120	48	-0.6778190265997139
120	49	-0.6205829165878782
120	50	-0.4238059531709766
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	11845.0
3	948.0
4	1615.0
5	1928.0
6	2967.0
7	4799.0
8	5948.0
9	7216.0
10	10585.0
11	13821.0
12	17479.0
13	24515.0
14	30971.0
15	37184.0
16	46071.0
17	55158.0
18	64603.0
19	73588.0
20	93068.0
21	75015.0
22	82610.0
23	102780.0
24	132490.0
25	125991.0
26	175129.0
27	214906.0
28	254873.0
29	278012.0
30	352805.0
31	438626.0
32	545788.0
33	676201.0
34	946139.0
35	1388439.0
36	2087710.0
37	3401866.0
38	5205779.0
39	1.7005701E7
40	158324.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.398671147350726	19.05632218453681	14.859677236284636	23.68532943182783
2	17.344262286441488	20.83770544457384	34.49796860255334	27.32006366643133
3	17.729682876067756	24.29453701851228	34.483708591680504	23.49207151373946
4	11.79202373238954	15.748031980213561	35.43227628283877	37.02766800455813
5	12.763555399736127	37.05359214648997	38.04022329429087	12.142629159483043
6	34.26642481341513	35.46576041285148	16.776067384967035	13.491747388766356
7	26.65924683018513	35.01190053971932	21.305170747835366	17.023681882260185
8	31.412751837711006	32.10371776614474	19.50249129715663	16.981039098987623
9	25.69988743757154	12.246027434032221	19.02024249819023	43.03384263020602
10	19.79322876286768	26.277095581409494	33.64748958474028	20.28218607098255
11	37.334699557670426	20.46723595738802	22.42538413274449	19.772680352197064
12	23.928389989275768	25.283800400737928	30.443009152826622	20.344800457159682
13	29.219567673502677	19.295874070625807	24.95853937985201	26.526018876019503
14	20.4899832646693	19.724442240798034	31.119493399986936	28.66608109454573
15	27.543438310788247	27.108030417504597	23.152071829141217	22.19645944256593
16	26.57057361599881	25.662446883544238	25.015488166905797	22.751491333551154
17	20.925399929078996	26.41658643817193	26.394316388077787	26.263697244671285
18	21.542445184072864	29.259016195301413	27.251370475037305	21.94716814558842
19	21.498314120431612	29.023436589577305	28.00170479440046	21.47654449559062
20	26.87442247854414	24.711396283829593	27.472419878107345	20.941761359518924
21	26.92498588180254	24.672920007480514	27.191670264963584	21.21042384575336
22	22.404928730622196	24.346077860878065	27.46399492313762	25.78499848536212
23	21.934941641569242	28.89029999379848	27.246720967170408	21.928037397461875
24	25.507893532356025	24.919371707758586	27.752620090098024	21.820114669787365
25	22.0449390804556	29.313021390809713	27.30429068475168	21.33774884398301
26	22.081557177079514	24.700454637795964	27.34630609793806	25.87168208718646
27	21.193011952532483	24.852424326731107	27.444767636742867	26.509796083993546
28	25.654152357574763	25.15196146393719	27.58084995456203	21.613036223926013
29	21.127190700651656	29.28387944295198	27.811561177232036	21.777368679164326
30	21.738593474544484	25.363591384913537	31.494955704223226	21.402859436318753
31	22.001522977265928	28.241181902654745	27.555328664368066	22.201966455711265
32	20.928704416106438	25.033585491959254	32.03059134051083	22.007118751423473
33	21.24212414903368	25.412993245321847	27.588069681320366	25.75681292432411
34	25.981376333677318	24.530216904187345	27.96419505045669	21.524211711678646
35	21.336527062494913	25.21584567628688	31.96518136794945	21.48244589326876
36	21.62048416560923	29.72925519407312	27.076299553381972	21.573961086935682
37	21.469668167562087	24.768912022828218	32.07865101842333	21.682768791186366
38	26.089158595888772	25.62662207050771	27.022711140046873	21.26150819355665
39	21.310211512152076	25.528533432175255	27.62640680993258	25.53484824574009
40	21.504152071960405	24.70083713056178	27.971405936911705	25.823604860566114
41	25.59124241788458	25.258207174194013	27.261513113145064	21.88903729477634
42	20.96976138956579	25.10631191472231	32.11718836780093	21.80673832791097
43	21.614199876161543	24.966755226234945	27.324366523818313	26.094678373785197
44	21.58190308055275	25.098083935564393	31.901435495969903	21.418577487912952
45	21.225428102385884	25.107520911570546	31.817890905031504	21.84916008101206
46	21.510893258797818	25.67538345937616	27.05615917295143	25.757564108874586
47	21.21118294191014	25.396758953499948	31.985394316915812	21.406663787674102
48	26.17212071981835	25.394691138837487	26.311217905180932	22.12197023616323
49	21.31793591812948	25.404720634372218	27.193871860676577	26.083471586821727
50	21.544958835853905	25.35521278612659	30.942032071732257	22.15779630628725
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	14929.0
1	12990.0
2	11051.0
3	26207.5
4	41364.0
5	42706.0
6	44048.0
7	34114.5
8	24181.0
9	33144.5
10	42108.0
11	65734.5
12	89361.0
13	146347.5
14	203334.0
15	205951.5
16	208569.0
17	272608.0
18	336647.0
19	325574.5
20	314502.0
21	351985.0
22	389468.0
23	438761.5
24	488055.0
25	539105.5
26	590156.0
27	665819.5
28	741483.0
29	820460.0
30	899437.0
31	990635.5
32	1081834.0
33	1158346.0
34	1234858.0
35	1324886.0
36	1414914.0
37	1488609.0
38	1562304.0
39	1616660.5
40	1671017.0
41	1711648.0
42	1752279.0
43	1777758.0
44	1803237.0
45	1809624.5
46	1816012.0
47	1805101.0
48	1794190.0
49	1786998.5
50	1779807.0
51	1758074.0
52	1736341.0
53	1771976.0
54	1807611.0
55	1793642.5
56	1779674.0
57	2211793.0
58	2643912.0
59	2201968.5
60	1760025.0
61	1449741.5
62	1139458.0
63	997734.5
64	856011.0
65	756110.5
66	656210.0
67	568172.5
68	480135.0
69	413254.5
70	346374.0
71	288923.0
72	231472.0
73	194462.5
74	157453.0
75	124468.0
76	91483.0
77	73238.5
78	54994.0
79	42715.0
80	30436.0
81	23285.5
82	16135.0
83	12427.0
84	8719.0
85	6622.5
86	4526.0
87	3502.5
88	2479.0
89	1534.0
90	589.0
91	390.0
92	191.0
93	125.0
94	59.0
95	42.0
96	25.0
97	21.0
98	17.0
99	18.0
100	19.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.06557162396244506
2	0.006945116858179045
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	2.92795820327953E-6
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	2.92795820327953E-6
16	0.0
17	0.0
18	2.0495707422956707E-5
19	0.0011741112395150916
20	0.0
21	0.002231104150899002
22	3.367151933771459E-4
23	0.0017187114653250842
24	0.0013732123973380994
25	0.022841001943783613
26	0.06226888710914576
27	0.11506875738888553
28	0.1298608022318537
29	0.10920112914951335
30	0.16561409985210004
31	0.20954225677590282
32	0.18846974158690005
33	0.2166718350008885
34	0.2809873648941266
35	0.3358543736653818
36	0.360346744035815
37	0.3631517279945568
38	0.3813197086459063
39	0.40475215814675236
40	0.42752288909365727
41	0.45643940430924584
42	0.519791635953605
43	0.47560581870791374
44	0.5062527572216405
45	0.5423076345368247
46	0.6005593629910709
47	0.6044271957776032
48	0.7168432230343175
49	0.713130572032559
50	0.7283149632747666
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	3.4153493E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.130104598424836
#Duplication Level	Percentage of deduplicated	Percentage of total
1	66.54601568269482	23.377684915414882
2	13.92946159547539	9.786868856975842
3	6.602398569812361	6.958288570739963
4	3.647048702594577	5.124848095907883
5	2.263405420803662	3.97568345907372
6	1.5054857591560995	3.173272331435567
7	1.0126440593627333	2.4902004208470436
8	0.7733218499134377	2.173350198056516
9	0.5704023876589369	1.8034465987484753
>10	2.819689842133024	17.284261818662504
>50	0.185267815859924	4.529505282174962
>100	0.13112258623879006	8.846594249338988
>500	0.00987667037197358	2.340685227300329
>1k	0.003600925983970816	2.0559522112901014
>5k	7.49415313322017E-5	0.16735513219853063
>10k+	1.8319040917733957E-4	5.912002631834739
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TCTCTGAGCGGGCTGGCAAGGCAGACCGATATCGTATGCCGTCTTCTGCT	966754	2.830615304853299	Illumina PCR Primer Index 1 (95% over 24bp)
TCTCTGAGCGGGCTGGCAAGGCAGACCGATCTCGTATGCCGTCTTCTGCT	304881	0.8926788249740664	Illumina Paired End PCR Primer 2 (96% over 29bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	110556	0.3237033471217717	No Hit
CTTATACACATCTCTGAGCGGGCTGGCAAGGCAGACCGATATCGTATGCC	98983	0.2898180868352177	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	94345	0.27623821668840726	No Hit
CATCTCTGAGCGGGCTGGCAAGGCAGACCGATATCGTATGCCGTCTTCTG	64729	0.18952380654008066	Illumina Paired End PCR Primer 2 (96% over 26bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	59202	0.17334098155055472	No Hit
ATACACATCTCTGAGCGGGCTGGCAAGGCAGACCGATATCGTATGCCGTC	39533	0.11575097165024965	No Hit
ACACATCTCTGAGCGGGCTGGCAAGGCAGACCGATATCGTATGCCGTCTT	36183	0.10594231166926323	No Hit
CTTATACACATCTCTGAGCGGGCTGGCAAGGCAGACCGATCTCGTATGCC	35867	0.1050170768770269	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	8.78387460983859E-6	0.0	0.0	2.92795820327953E-6	5.85591640655906E-6
2	6.734303867542919E-5	0.0	0.0	4.391937304919295E-5	8.78387460983859E-6
3	7.027099687870872E-5	5.85591640655906E-6	0.0	4.391937304919295E-5	8.78387460983859E-6
4	7.027099687870872E-5	5.85591640655906E-6	0.0	6.734303867542919E-5	8.78387460983859E-6
5	2.3130869805908287E-4	2.049570742295671E-5	0.0	7.612691328526777E-5	8.78387460983859E-6
6	4.655453543214453E-4	2.049570742295671E-5	0.0	7.905487148854731E-5	8.78387460983859E-6
7	6.382948883149375E-4	2.049570742295671E-5	0.0	9.955057891150401E-5	8.78387460983859E-6
8	0.001077488618806867	2.049570742295671E-5	0.0	1.0540649531806307E-4	8.78387460983859E-6
9	0.0011096961590429419	2.049570742295671E-5	0.0	1.6103770118037415E-4	8.78387460983859E-6
10	0.0016601523012594935	2.049570742295671E-5	0.0	2.1374094883940567E-4	1.171183281311812E-5
11	0.0018036222532201904	2.6351623829515767E-5	0.0	2.3423665626236238E-4	1.171183281311812E-5
12	0.001879749166505458	2.6351623829515767E-5	0.0	2.664441964984372E-4	1.171183281311812E-5
13	0.002784488251318833	3.806345664263389E-5	0.0	3.1914744415746876E-4	1.4639791016397649E-5
14	0.006614257581208458	3.806345664263389E-5	0.0	3.92346399239457E-4	1.4639791016397649E-5
15	0.007076874977326624	3.806345664263389E-5	0.0	4.2748189767881134E-4	1.4639791016397649E-5
16	0.008397384127005691	3.806345664263389E-5	0.0	4.3626577228864994E-4	1.4639791016397649E-5
17	0.008400312085208972	4.099141484591342E-5	0.0	4.567614797116067E-4	1.4639791016397649E-5
18	0.008417879834428649	4.977528945575201E-5	0.0	4.6847331252472475E-4	1.4639791016397649E-5
19	0.00847351104029096	5.2703247659031534E-5	0.0	5.006808527607996E-4	1.4639791016397649E-5
20	0.00859355732662542	5.2703247659031534E-5	0.0	5.387443094034335E-4	1.4639791016397649E-5
21	0.008941984352815685	6.734303867542919E-5	0.0	5.680238914362288E-4	1.4639791016397649E-5
22	0.0089683359766452	6.734303867542919E-5	0.0	5.855916406559059E-4	1.756774921967718E-5
23	0.008985903725864877	7.612691328526777E-5	0.0	6.090153062821422E-4	1.756774921967718E-5
24	0.009009327391491113	7.612691328526777E-5	0.0	6.500067211280557E-4	1.756774921967718E-5
25	0.009135229594232134	7.612691328526777E-5	0.0	7.232056762100439E-4	1.756774921967718E-5
26	0.009518792118861752	7.905487148854731E-5	0.0	7.671250492592368E-4	1.756774921967718E-5
27	0.009814515897392984	7.905487148854731E-5	0.0	0.0011975349051413277	1.756774921967718E-5
28	0.010168798839989807	7.905487148854731E-5	0.0	0.001812406127830029	1.756774921967718E-5
29	0.010180510672802925	8.198282969182684E-5	0.0	0.0028811108720270574	1.756774921967718E-5
30	0.010221502087648838	8.198282969182684E-5	0.0	0.004283602851397952	1.756774921967718E-5
31	0.010227358004055397	8.198282969182684E-5	0.0	0.0060725853136017445	1.756774921967718E-5
32	0.010233213920461956	8.198282969182684E-5	0.0	0.008707747696553322	2.049570742295671E-5
33	0.010236141878665237	9.369466250494496E-5	2.92795820327953E-6	0.013494959358915353	2.9279582032795297E-5
34	0.010244925753275074	9.369466250494496E-5	2.92795820327953E-6	0.020100433065513974	2.9279582032795297E-5
35	0.010250781669681634	9.369466250494496E-5	2.92795820327953E-6	0.029027777627313258	2.9279582032795297E-5
36	0.010262493502494752	9.369466250494496E-5	2.92795820327953E-6	0.04326058245345506	2.9279582032795297E-5
37	0.010262493502494752	9.369466250494496E-5	2.92795820327953E-6	0.06848787033291148	2.9279582032795297E-5
38	0.01026834941890131	1.083344535213426E-4	2.92795820327953E-6	0.10403035496252169	2.9279582032795297E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTCTGCT	167130	0.0	37.465847	44
CGTCTTC	165680	0.0	37.16849	40
CTTCTGC	171520	0.0	36.521145	43
GCCGTCT	169965	0.0	36.272903	38
CCGTCTT	169985	0.0	36.25875	39
GTCTTCT	173840	0.0	35.447826	41
TATGCCG	177340	0.0	35.06434	35
ATGCCGT	176815	0.0	34.969067	36
TGCCGTC	176810	0.0	34.87534	37
TCTTCTG	180095	0.0	34.236156	42
ACCGATA	137555	0.0	32.885654	25
TATCGTA	136340	0.0	32.76536	30
CCGATAT	137455	0.0	32.7651	26
ATATCGT	136245	0.0	32.760822	29
GACCGAT	189575	0.0	32.729416	24
TGAGCGG	201680	0.0	32.71275	5
ATCGTAT	138415	0.0	32.701202	31
CGATATC	137960	0.0	32.658657	27
GCGGGCT	201940	0.0	32.606506	8
GATATCG	138350	0.0	32.601692	28
>>END_MODULE