SRR522143_1.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	SRR522143_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	32360030
Sequences flagged as poor quality	0
Sequence length	50
%GC	47

Per base quality graph

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
TCTCTGAGCGGGCTGGCAAGGCAGACCGATATCGTATGCCGTCTTCTGCT	1348618	4.167542489917345	Illumina PCR Primer Index 1 (95% over 24bp)
TCTCTGAGCGGGCTGGCAAGGCAGACCGATCTCGTATGCCGTCTTCTGCT	376630	1.1638740755184713	Illumina Paired End PCR Primer 2 (96% over 29bp)
CTTATACACATCTCTGAGCGGGCTGGCAAGGCAGACCGATATCGTATGCC	141557	0.4374439702311772	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	108016	0.33379449895442	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	95339	0.2946196279793313	No Hit
CATCTCTGAGCGGGCTGGCAAGGCAGACCGATATCGTATGCCGTCTTCTG	87921	0.2716962870553581	Illumina Paired End PCR Primer 2 (96% over 26bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	58387	0.1804293753744975	No Hit
ATACACATCTCTGAGCGGGCTGGCAAGGCAGACCGATATCGTATGCCGTC	56609	0.17493494289096764	No Hit
ACACATCTCTGAGCGGGCTGGCAAGGCAGACCGATATCGTATGCCGTCTT	51381	0.1587792100316347	No Hit
CTTATACACATCTCTGAGCGGGCTGGCAAGGCAGACCGATCTCGTATGCC	46239	0.14288923712369858	No Hit
CATCTCTGAGCGGGCTGGCAAGGCAGACCGATCTCGTATGCCGTCTTCTG	35168	0.10867727872934604	Illumina Paired End PCR Primer 2 (100% over 26bp)

Adapter graph

Kmer graph

Sequence	Count	Obs/Exp Max	Max Obs/Exp Position
TTCTGCT	218215	38.802322	44
CTTCTGC	222180	38.09532	43
CGTCTTC	220970	37.743248	40
CCGTCTT	227405	36.74603	39
GCCGTCT	227665	36.714954	38
GTCTTCT	228605	36.48702	41
TCTTCTG	233655	35.69955	42
TATGCCG	237755	35.39259	35
ATGCCGT	237415	35.29622	36
TGCCGTC	236855	35.28887	37
ACCGATA	191700	33.056286	25
TATCGTA	189885	33.0099	30
CCGATAT	191425	32.97804	26
ATATCGT	190065	32.97049	29
ATCGTAT	192265	32.91583	31
CGATATC	191990	32.90139	27
TGAGCGG	269890	32.891453	5
GATATCG	192165	32.88559	28
GACCGAT	256095	32.87293	24
GCGGGCT	270780	32.712833	8