FastQCFastQC Report
Thu 26 May 2016
SRR522142_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR522142_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27541899
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT605150.21971978039713236No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT529020.19207825865602077No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT329020.11946162463234652No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA291350.032.6261941
TATGCCG62150.028.67146335
TCGTATG64950.027.12823132
TATCGTA49000.026.84404630
ATATCGT49250.026.84152229
CGTATGC66900.026.63342533
GACCGAT67550.026.07544124
ATCGTAT51900.025.59819231
ACCGATA52000.025.41541725
CGATATC54900.024.31463227
GATATCG56250.023.69555728
CCGATAT57600.023.09747526
AGACCGA78700.022.93764523
GCCGTCT80800.022.49001138
ATGCCGT79200.022.35915436
CCGTCTT82050.022.28152839
GCGGGCT84400.021.8791528
TGAGCGG86200.021.8556255
GTATCAA434000.021.7667142
TGCCGTC88950.020.10649537
Produced by FastQC (version 0.11.5)