##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR522142_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	27541899
Sequences flagged as poor quality	0
Sequence length	50
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	39.05362317246171	40.0	39.0	40.0	38.0	40.0
2	38.60824026694746	40.0	39.0	40.0	38.0	40.0
3	38.803839669878975	40.0	39.0	40.0	37.0	40.0
4	38.884702176854255	40.0	39.0	40.0	38.0	40.0
5	38.88996750006236	40.0	39.0	40.0	38.0	40.0
6	38.98134776400131	40.0	39.0	40.0	38.0	40.0
7	38.93224563055728	40.0	39.0	40.0	38.0	40.0
8	38.92944237432575	40.0	39.0	40.0	38.0	40.0
9	38.97392638031241	40.0	39.0	40.0	38.0	40.0
10	38.919312608037664	40.0	39.0	40.0	37.0	40.0
11	39.04062552113781	40.0	39.0	40.0	38.0	40.0
12	38.97360290951615	40.0	39.0	40.0	38.0	40.0
13	38.97732487509303	40.0	39.0	40.0	38.0	40.0
14	38.936640280323445	40.0	39.0	40.0	38.0	40.0
15	38.92739062763973	40.0	39.0	40.0	37.0	40.0
16	38.91433299497613	40.0	39.0	40.0	37.0	40.0
17	38.87911116804255	40.0	39.0	40.0	37.0	40.0
18	38.85067765298246	40.0	39.0	40.0	37.0	40.0
19	38.82146020504977	40.0	39.0	40.0	37.0	40.0
20	38.796154760425196	40.0	39.0	40.0	36.0	40.0
21	38.738514726235834	40.0	39.0	40.0	36.0	40.0
22	38.66464211491009	40.0	39.0	40.0	36.0	40.0
23	38.64903393190136	40.0	39.0	40.0	36.0	40.0
24	38.60030856986295	40.0	39.0	40.0	36.0	40.0
25	38.578607306634886	40.0	39.0	40.0	36.0	40.0
26	38.49737547145896	40.0	39.0	40.0	36.0	40.0
27	38.42930670829924	40.0	38.0	40.0	35.0	40.0
28	38.3785122805076	40.0	38.0	40.0	35.0	40.0
29	38.48543686112566	40.0	39.0	40.0	36.0	40.0
30	38.44246771074137	40.0	39.0	40.0	35.0	40.0
31	38.40321943668445	40.0	39.0	40.0	36.0	40.0
32	38.35305060119493	40.0	39.0	40.0	35.0	40.0
33	38.299590453076604	40.0	38.0	40.0	35.0	40.0
34	38.264647619250944	40.0	38.0	40.0	35.0	40.0
35	38.18939979411006	40.0	38.0	40.0	35.0	40.0
36	38.13776388476336	40.0	38.0	40.0	35.0	40.0
37	38.070596112490286	40.0	38.0	40.0	35.0	40.0
38	38.18933810627946	40.0	39.0	40.0	35.0	40.0
39	38.11342627463706	40.0	38.0	40.0	35.0	40.0
40	38.049580677062245	40.0	38.0	40.0	35.0	40.0
41	38.0749247174278	40.0	39.0	40.0	35.0	40.0
42	38.00539552483291	40.0	39.0	40.0	35.0	40.0
43	37.934252028155356	40.0	38.0	40.0	35.0	40.0
44	37.83393654155801	40.0	38.0	40.0	35.0	40.0
45	37.73518449109119	40.0	38.0	40.0	35.0	40.0
46	37.519764668369454	40.0	38.0	40.0	35.0	40.0
47	37.413668825087186	40.0	38.0	40.0	34.0	40.0
48	37.338291306637934	40.0	38.0	40.0	34.0	40.0
49	37.26785535013399	40.0	38.0	40.0	33.0	40.0
50	37.202955794732965	40.0	38.0	40.0	33.0	40.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1	1	-22.862475929047307
1	2	-24.007926679176077
1	3	-31.726431325034838
1	4	-31.042043136589154
1	5	-29.179999712444857
1	6	-30.825733547528422
1	7	-30.412495369170944
1	8	-29.83517789210388
1	9	-29.825154859599678
1	10	-29.324182546810285
1	11	-27.810772761892004
1	12	-30.69803082198378
1	13	-26.415805787059128
1	14	-26.66963051113072
1	15	-27.826685496812452
1	16	-26.942520543236363
1	17	-28.66942067330328
1	18	-26.438500825296245
1	19	-26.66733391628075
1	20	-27.26297259293859
1	21	-26.96967422527075
1	22	-29.137964581345074
1	23	-25.813450783350696
1	24	-25.844354462187177
1	25	-25.820522006054794
1	26	-25.324589009059657
1	27	-24.73763862721948
1	28	-25.197830313446396
1	29	-25.646449261458052
1	30	-25.012639532387382
1	31	-25.887182671464075
1	32	-26.589140056332965
1	33	-25.40240494063646
1	34	-28.4912272272192
1	35	-26.786013471763837
1	36	-29.751857545497703
1	37	-31.612767441402767
1	38	-29.558106613759946
1	39	-28.969005261782293
1	40	-29.045043792826597
1	41	-26.67559537855216
1	42	-27.190794727706702
1	43	-26.201651714998754
1	44	-27.10902253091134
1	45	-26.41291927771318
1	46	-24.924061291290165
1	47	-24.782656567269385
1	48	-24.985424421658283
1	49	-25.05177487680141
1	50	-26.9059402624744
2	1	0.3819969815156341
2	2	0.7140708730038554
2	3	0.6479947389097802
2	4	0.5739395199148092
2	5	0.5616792439006844
2	6	0.5255221453361614
2	7	0.5519345782078631
2	8	0.480475586536393
2	9	0.47039303553680156
2	10	0.4608890349839925
2	11	0.35972392530410957
2	12	0.40091110663551177
2	13	0.3666405641888488
2	14	0.3841630134639047
2	15	0.35864394936700705
2	16	0.3349113423213126
2	17	0.35636998387349195
2	18	0.3243697697038428
2	19	0.33578213026802217
2	20	0.293244777946974
2	21	0.35156493907010145
2	22	0.41764063039508414
2	23	0.32094062589953865
2	24	0.33303578566174963
2	25	0.3073714892314925
2	26	0.31756275914128196
2	27	0.31422465684490675
2	28	0.2826903017102609
2	29	0.41650129300299454
2	30	0.4110241768180174
2	31	0.47876157483192827
2	32	0.4337616814414673
2	33	0.4203699742747773
2	34	0.42430932652939646
2	35	0.3951971572059847
2	36	0.37079723743393345
2	37	0.38240246644131304
2	38	0.3813533189837415
2	39	0.3321895576502669
2	40	0.3621621118804299
2	41	0.32600013635124725
2	42	0.3389754628963857
2	43	0.2691444380017529
2	44	0.3095405476317836
2	45	0.2884962093280308
2	46	0.2967912579206029
2	47	0.28938565052763465
2	48	0.2252508626849874
2	49	0.2350395781018335
2	50	0.23714335302143752
3	1	0.4142777858245168
3	2	0.755434830006422
3	3	0.67815618045109
3	4	0.6165059788964342
3	5	0.5940563911205174
3	6	0.5573487055279571
3	7	0.5914775933915735
3	8	0.5233596398805105
3	9	0.48667559928303206
3	10	0.48592906774640454
3	11	0.379154448630338
3	12	0.41083707758264154
3	13	0.3715691020961813
3	14	0.4141912796529539
3	15	0.37558049759957157
3	16	0.3403778234533803
3	17	0.3556819124789996
3	18	0.3560597216828114
3	19	0.3426620096011206
3	20	0.33061910140295225
3	21	0.36130152487756817
3	22	0.42256351106904333
3	23	0.3558888459562297
3	24	0.42195164799766616
3	25	0.370519333325376
3	26	0.3628884487290449
3	27	0.3585154994725599
3	28	0.3093899393976969
3	29	0.4715615555107817
3	30	0.4285758453255326
3	31	0.5016111668632632
3	32	0.47119471096739574
3	33	0.46404520190279896
3	34	0.4408600725944183
3	35	0.45054371046649777
3	36	0.40502366928734546
3	37	0.40743294082076176
3	38	0.359902547769714
3	39	0.346748953791284
3	40	0.4051485467978182
3	41	0.3357679766736723
3	42	0.35939866828911704
3	43	0.3236543023077232
3	44	0.35496867166416024
3	45	0.33923239307539177
3	46	0.3525449431577954
3	47	0.3556266115158948
3	48	0.2899642875788686
3	49	0.28965643511721595
3	50	0.3179207073413224
4	1	0.4706408621989624
4	2	0.7658674104101237
4	3	0.7183569705073296
4	4	0.6468663468431544
4	5	0.6345317585030728
4	6	0.566364280482837
4	7	0.6047129212305151
4	8	0.5472270695000248
4	9	0.4896197119395822
4	10	0.49721041633731033
4	11	0.3801371278344945
4	12	0.43477418384211575
4	13	0.3936800619031331
4	14	0.39670058011547127
4	15	0.37261490496324257
4	16	0.3706301478341274
4	17	0.35416975993151567
4	18	0.37392773988896977
4	19	0.3638403202232112
4	20	0.3615864371257018
4	21	0.3603796188945054
4	22	0.47657190809535166
4	23	0.3679364705672512
4	24	0.41711501537849927
4	25	0.37037478188779005
4	26	0.3828643597006476
4	27	0.3397760639636047
4	28	0.38667873909925277
4	29	0.45753788453452415
4	30	0.44517985299358287
4	31	0.48732196073412837
4	32	0.5117050689145373
4	33	0.48516754046919175
4	34	0.4850292384880177
4	35	0.44484509971023556
4	36	0.4200761926236538
4	37	0.41763744723854757
4	38	0.43976622134387355
4	39	0.3724792638534993
4	40	0.3734523705266284
4	41	0.39390527959257327
4	42	0.38624024322307804
4	43	0.3476053444090894
4	44	0.3677902282076886
4	45	0.31536546451408043
4	46	0.33398926881804414
4	47	0.35176954126190907
4	48	0.27525348593775334
4	49	0.3331661364674119
4	50	0.3594614632203559
5	1	0.3678485277446981
5	2	0.6835400626302004
5	3	0.6733060309310872
5	4	0.6059054045145515
5	5	0.5657466411817111
5	6	0.5210748096387761
5	7	0.58652729438937
5	8	0.5243718812037059
5	9	0.49396076175052883
5	10	0.4999056019702337
5	11	0.365021043402443
5	12	0.3944150630400429
5	13	0.3348353513264115
5	14	0.40337998545705744
5	15	0.35137930718730814
5	16	0.3419170969198859
5	17	0.3179712768400833
5	18	0.32209719582564134
5	19	0.36056697152239536
5	20	0.30224717527882206
5	21	0.3499655013600318
5	22	0.430046006745755
5	23	0.34278390647700263
5	24	0.33295442879440884
5	25	0.3462929514395938
5	26	0.3373434339326593
5	27	0.2782311689168395
5	28	0.2991585375700083
5	29	0.41728482777898535
5	30	0.40725969504278936
5	31	0.4912155668716238
5	32	0.44815290985751943
5	33	0.43898194205378616
5	34	0.3993545380241983
5	35	0.3927869888527553
5	36	0.36577021290610645
5	37	0.37293549740059717
5	38	0.41168741309761714
5	39	0.337406522544498
5	40	0.37547705000919507
5	41	0.32721015631625505
5	42	0.3407420444371425
5	43	0.3023705310934304
5	44	0.35278635268946346
5	45	0.34063356702364445
5	46	0.4053721958381473
5	47	0.3921466767164574
5	48	0.3662867762619868
5	49	0.34036218424994047
5	50	0.34008979718699095
6	1	0.4375838993671266
6	2	0.763004068950174
6	3	0.7158652341536609
6	4	0.651732191257004
6	5	0.6303810015075513
6	6	0.5823930486220377
6	7	0.6031897436220746
6	8	0.5380352422660977
6	9	0.4903170781632795
6	10	0.4858148006030518
6	11	0.4031999803598296
6	12	0.40157864755697403
6	13	0.4001046987901944
6	14	0.4232177667209598
6	15	0.3734473261399742
6	16	0.3793272834819632
6	17	0.36092143292797374
6	18	0.37545023157559854
6	19	0.40366246884385504
6	20	0.3020845151223739
6	21	0.40143426552361205
6	22	0.49284376055592105
6	23	0.3661390144525001
6	24	0.409805936049338
6	25	0.40259925587943
6	26	0.37361052354295765
6	27	0.3929131688914822
6	28	0.36138529538032316
6	29	0.4990189781731402
6	30	0.43793038650542826
6	31	0.5026004166502034
6	32	0.4810605527938705
6	33	0.4722225839193257
6	34	0.4886266675331328
6	35	0.4462156497652927
6	36	0.3932854134336736
6	37	0.3923383038675965
6	38	0.4236389431573997
6	39	0.3972898491326191
6	40	0.41735627532744957
6	41	0.36523703024823106
6	42	0.4448541007987288
6	43	0.3832591329189654
6	44	0.4307304493762629
6	45	0.4525423014084424
6	46	0.48351736177666993
6	47	0.48551994397893594
6	48	0.43482081675504247
6	49	0.35718428304501515
6	50	0.37996404691401864
7	1	0.4572143214497757
7	2	0.781180234449451
7	3	0.6921153420052235
7	4	0.6715578188211211
7	5	0.6311062092740158
7	6	0.5720417991646372
7	7	0.6107638481984452
7	8	0.5608973012863032
7	9	0.5198010059743865
7	10	0.49430034189651906
7	11	0.42066128399407177
7	12	0.4438109264459271
7	13	0.4033952854286511
7	14	0.45179133014795525
7	15	0.4078182201273819
7	16	0.38721614981189134
7	17	0.375745407825292
7	18	0.3799766431556151
7	19	0.4032359964260479
7	20	0.3730337993487467
7	21	0.41050968397972554
7	22	0.48606214412534143
7	23	0.3788201137647391
7	24	0.40902890612186127
7	25	0.3912157367161697
7	26	0.4078979213505889
7	27	0.39024415796045986
7	28	0.39454031636012843
7	29	0.4651826960037866
7	30	0.4766297178739194
7	31	0.4816756150668624
7	32	0.47973241319842685
7	33	0.45808311718681693
7	34	0.40328106080230697
7	35	0.39982809960420695
7	36	0.3991643080668865
7	37	0.41167248817005486
7	38	0.3373438952471588
7	39	0.3711997503274631
7	40	0.3714766022377134
7	41	0.2803894411204766
7	42	0.3061532170681289
7	43	0.28212320349416586
7	44	0.2524813730272868
7	45	0.2824154185131462
7	46	0.31319410841298634
7	47	0.2901311330880816
7	48	0.25029885264546436
7	49	0.2329411786261204
7	50	0.2418809305417824
8	1	0.45254114337986096
8	2	0.7840200236595933
8	3	0.7378180553759037
8	4	0.6856427368520102
8	5	0.6406279340006122
8	6	0.6086373217573566
8	7	0.6312143913689354
8	8	0.5582232511167149
8	9	0.5258460065574155
8	10	0.5247728978630946
8	11	0.4417703233698447
8	12	0.4740551733256595
8	13	0.42245577293497405
8	14	0.45811622018918285
8	15	0.41269611888414914
8	16	0.3936114199812266
8	17	0.4006790424835671
8	18	0.37979417411938954
8	19	0.42456372084347294
8	20	0.351137502624681
8	21	0.43055164668709267
8	22	0.5131244448468877
8	23	0.4197697824178519
8	24	0.4402052152355651
8	25	0.39612617954343676
8	26	0.4184719153585519
8	27	0.38612650788901703
8	28	0.4256331061448435
8	29	0.5050654076358114
8	30	0.48535289164561135
8	31	0.49469541754514523
8	32	0.4518233780342342
8	33	0.47401824621534416
8	34	0.4438557478845553
8	35	0.4007943342123994
8	36	0.38118040850311985
8	37	0.40111118640459154
8	38	0.3481993740108962
8	39	0.3556660875600457
8	40	0.33758753635942185
8	41	0.2603261083019106
8	42	0.24280698149978974
8	43	0.12586433979392808
8	44	-0.019151630834372213
8	45	0.016136203051665632
8	46	0.050953518032301304
8	47	-0.06775289793205275
8	48	-0.24009233725877976
8	49	-0.18427870377437472
8	50	-0.29626957948007515
9	1	0.4379628803489908
9	2	0.7556874751384797
9	3	0.6744926186868838
9	4	0.6216083283342968
9	5	0.614899049990207
9	6	0.5643156552344877
9	7	0.6318205716661964
9	8	0.5212010409958907
9	9	0.500083405273827
9	10	0.4822549311660893
9	11	0.4101578630317704
9	12	0.4362224379945161
9	13	0.3879544860121058
9	14	0.39769185494034076
9	15	0.3890979512566233
9	16	0.36749123322584865
9	17	0.3614652319152398
9	18	0.37155983095590983
9	19	0.3816650844312406
9	20	0.3327765525086761
9	21	0.39316373340510324
9	22	0.4193310165333344
9	23	0.3351100346289684
9	24	0.3667190276611123
9	25	0.3528231310568728
9	26	0.3377701783403424
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119	11	0.41474602016411666
119	12	0.41785735115848865
119	13	0.4090753054193357
119	14	0.4203780507219861
119	15	0.3966912653666981
119	16	0.3885757108078636
119	17	0.37900391094732555
119	18	0.37962725397930797
119	19	0.39480575692832076
119	20	0.3948554381257168
119	21	0.4440067556829064
119	22	0.5080609183383586
119	23	0.42584103409713236
119	24	0.4618072604064736
119	25	0.4587884917989342
119	26	0.42991100546180405
119	27	0.4450545808033368
119	28	0.44872399971759336
119	29	0.5612643521200908
119	30	0.5451677039464755
119	31	0.5854722115220028
119	32	0.5535723432511404
119	33	0.5357414008269572
119	34	0.5174231947744232
119	35	0.5646938162664128
119	36	0.4839735641160843
119	37	0.5095589196936601
119	38	0.46570523458954227
119	39	0.4770938442766379
119	40	0.47236153596829666
119	41	0.46173469422939917
119	42	0.4984258184163579
119	43	0.460437075015129
119	44	0.4891057574732329
119	45	0.4575360107120545
119	46	0.5069799670814561
119	47	0.5161293689872153
119	48	0.46978472802311444
119	49	0.47133503903221197
119	50	0.5442081700792301
120	1	-9.89049227508771
120	2	-10.849580745239404
120	3	-27.917870689820848
120	4	-23.018469273620056
120	5	-18.09382919461932
120	6	-19.7342303432976
120	7	-23.787900427265107
120	8	-17.9895539417873
120	9	-14.086959569414656
120	10	-14.691414855610606
120	11	-9.774917425081025
120	12	-9.195992849637246
120	13	-8.69674065768639
120	14	-15.55530291061475
120	15	-11.640670520753261
120	16	-9.198270461659224
120	17	-7.534061951295005
120	18	-6.332000972873317
120	19	-7.24725709492974
120	20	-8.250936019831318
120	21	-7.806128891611785
120	22	-14.076867943488107
120	23	-7.073449756609783
120	24	-10.42417239312002
120	25	-8.178945067468394
120	26	-7.3996042171514596
120	27	-6.797571715679169
120	28	-6.693970350070437
120	29	-7.50903692217247
120	30	-7.627891131996893
120	31	-6.40749771567015
120	32	-6.514359454475862
120	33	-7.586872084453198
120	34	-6.323080116648278
120	35	-9.27812822347066
120	36	-6.786233860670862
120	37	-7.477727848891583
120	38	-6.84292819991753
120	39	-8.016030537353188
120	40	-7.7152393056979065
120	41	-8.09637818587041
120	42	-9.282061429347578
120	43	-8.139850581167952
120	44	-8.698590572340741
120	45	-8.346126801421345
120	46	-13.93573417055207
120	47	-15.477577301651916
120	48	-10.597833791116102
120	49	-11.750862851836704
120	50	-10.881412397362688
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	20052.0
3	2153.0
4	992.0
5	912.0
6	1292.0
7	1846.0
8	2315.0
9	2861.0
10	3767.0
11	5069.0
12	6736.0
13	8423.0
14	10016.0
15	11083.0
16	11351.0
17	12903.0
18	14592.0
19	17196.0
20	21056.0
21	22367.0
22	28444.0
23	40393.0
24	54800.0
25	55995.0
26	82384.0
27	105387.0
28	124562.0
29	134875.0
30	170244.0
31	208980.0
32	256807.0
33	303343.0
34	419173.0
35	614105.0
36	936825.0
37	1701808.0
38	3447409.0
39	1.8226937E7
40	452446.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.19367460672283	19.233682892858106	10.86022857788077	24.712413922538296
2	18.246365646547165	21.403510044801536	35.54456452690452	24.805559781746783
3	18.652313543871603	24.821314855002097	31.025259313261866	25.501112287864437
4	12.54396305146657	15.811681396599026	37.48639435553378	34.15796119640062
5	13.482484927830846	38.49240257234979	35.139055070503325	12.886057429316041
6	32.20351432600233	36.94951591167246	17.316903246382896	13.530066515942316
7	28.61832239894398	32.017785151122006	22.151506006204666	17.212386443729347
8	28.483821773477164	33.32481055950618	20.28624102612551	17.905126640891144
9	27.36285757503075	12.705736965595838	19.677373067494905	40.25403239187851
10	15.902403824805253	27.08948645843193	35.47353434125948	21.534575375503337
11	35.20661374874695	21.471326287268717	23.11253483283778	20.20952513114655
12	20.55971334966339	26.025265261793617	32.081943085300594	21.3330783032424
13	30.06721101675521	20.236013571826074	25.679612491230042	24.017162920188674
14	21.441487602099507	20.248664207067048	27.90548328924956	30.40436490158388
15	24.11539990960657	28.53288148235934	23.628358818936608	23.723359789097483
16	23.32110890860474	27.013594400231938	25.65186694941208	24.013429741751235
17	21.862145145424165	27.194021352426873	27.43713110952734	23.506702392621623
18	22.269870054282777	26.476891469120577	28.11956770569625	23.133670770900395
19	22.945978397197337	26.15957384195113	28.520238843761668	22.374208917089867
20	23.61566278306276	25.81522350849466	28.167105587832186	22.402008120610397
21	23.606814475646722	25.840879744711863	27.67383614325214	22.87846963638927
22	23.143874523053757	25.70451234134711	28.121009714366714	23.030603421232414
23	23.091742474339792	25.89591590624186	27.857785758968273	23.15455586045007
24	22.92285547390469	26.041687544133147	28.01860207679596	23.016854905166195
25	23.132419347571066	25.93864347086521	28.03544159903732	22.89349558252641
26	22.811717010161267	25.861582029630213	28.072740613853142	23.25396034635538
27	22.619416539493294	26.001954643753212	28.07379422904396	23.304834587709536
28	22.99249616049442	25.801724424048743	28.260090108822123	22.945689306634716
29	22.637047897511156	26.074649811542223	28.324911617465116	22.963390673481506
30	22.707108628959645	26.277998655942486	28.015583774500502	22.999308940597366
31	23.00367405799833	26.29185609836612	28.011196852446822	22.693272991188724
32	22.639201857551253	26.21939388009974	28.22525623422136	22.91614802812765
33	22.65709760518258	26.143448900857198	28.17125100459601	23.02820248936421
34	23.09278814118593	25.706856665119737	28.240920882442598	22.959434311251734
35	22.797352320300803	25.984738044402544	28.41360583195287	22.804303803343778
36	22.970732094904296	26.60213350043657	27.685984056340985	22.741150348318154
37	23.095181779082402	25.999669739858216	28.013483068211865	22.891665412847512
38	23.1291321311789	26.57096854537759	27.70182449515794	22.598074828285576
39	22.790878059802385	26.48942535158905	27.841584339657928	22.87811224895064
40	22.80923448494686	26.043618086472996	28.091153406138513	23.055994022441634
41	22.834535234652915	26.173197368696837	27.907716006319294	23.084551390330954
42	22.552392308145823	26.48417886099772	27.99121658710669	22.97221224374977
43	22.79102979133796	26.123629190097684	27.981782971320495	23.103558047243858
44	22.775997014959316	26.42241631574238	27.903663922374992	22.89792274692331
45	22.66013635111717	26.439815357347552	27.943028339146796	22.95701995238848
46	22.972099704657417	26.467702548832726	27.493301158260486	23.06689658824937
47	22.66693722861162	26.684364353071423	27.804170402877197	22.844528015439757
48	22.882832075351526	26.691352154864507	27.252463598424203	23.173352171359767
49	22.789659995876764	26.606446649849623	27.49902823471396	23.10486511955965
50	22.824268290915093	26.520862736831564	27.508329802824488	23.146539169428856
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	8105.0
1	6735.0
2	5365.0
3	13685.5
4	22006.0
5	23081.0
6	24156.0
7	18811.0
8	13466.0
9	18826.5
10	24187.0
11	36916.0
12	49645.0
13	83832.0
14	118019.0
15	121682.5
16	125346.0
17	164729.5
18	204113.0
19	205198.5
20	206284.0
21	236067.5
22	265851.0
23	307019.5
24	348188.0
25	394611.0
26	441034.0
27	507964.0
28	574894.0
29	643752.0
30	712610.0
31	795239.0
32	877868.0
33	952378.5
34	1026889.0
35	1112398.5
36	1197908.0
37	1268264.5
38	1338621.0
39	1392646.5
40	1446672.0
41	1489255.0
42	1531838.0
43	1561400.0
44	1590962.0
45	1602953.0
46	1614944.0
47	1607111.0
48	1599278.0
49	1593783.0
50	1588288.0
51	1574179.5
52	1560071.0
53	1543330.0
54	1526589.0
55	1479674.0
56	1432759.0
57	1388204.5
58	1343650.0
59	1270018.0
60	1196386.0
61	1084569.5
62	972753.0
63	862056.5
64	751360.0
65	666490.0
66	581620.0
67	501683.0
68	421746.0
69	360474.5
70	299203.0
71	247529.5
72	195856.0
73	163654.5
74	131453.0
75	103544.0
76	75635.0
77	60056.5
78	44478.0
79	34349.0
80	24220.0
81	18558.0
82	12896.0
83	10063.5
84	7231.0
85	5569.5
86	3908.0
87	3046.0
88	2184.0
89	1403.5
90	623.0
91	512.0
92	401.0
93	294.0
94	187.0
95	136.5
96	86.0
97	58.0
98	30.0
99	33.5
100	37.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.05954927073111407
2	0.9141090815851152
3	6.535497062130684E-5
4	7.624746572485797E-5
5	2.795740409911459E-4
6	8.387221229734377E-4
7	1.706490899556345E-4
8	1.8154158505918564E-5
9	2.0695740696747162E-4
10	0.0
11	0.0
12	1.8517241676036937E-4
13	0.001568519294911364
14	6.535497062130684E-5
15	2.7231237758877847E-4
16	1.5975659485208336E-4
17	6.535497062130684E-5
18	5.0831643816571976E-5
19	0.0013797160464498108
20	4.6111562605033154E-4
21	0.0
22	3.993914871302084E-5
23	0.005776653236583287
24	8.822921033876422E-4
25	0.0017355375531658147
26	0.005921886504630635
27	0.012261318654897399
28	0.01160776894868433
29	0.012235902832989112
30	0.026733813815815678
31	0.02029271837791577
32	0.021283935432338925
33	0.030655112053094086
34	0.04404561936705962
35	0.02979460493991355
36	0.016088215267945034
37	0.021893915158137787
38	0.04052008178521024
39	0.03309866178799072
40	0.044528519983317055
41	0.04675785064784385
42	0.04789793180201554
43	0.05253087305272596
44	0.0555735100183179
45	0.04816298251620195
46	0.045432597076911795
47	0.050857059638480265
48	0.06842665424050826
49	0.07196671514916238
50	0.06367752637535996
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2.7541899E7
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.34870433662072
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.11391960514302	42.985572993306754
2	9.509303122360409	9.955993951994985
3	3.1513907835207564	4.949136731270388
4	1.4504116285019808	3.03708678027387
5	0.829790835778927	2.171923756171422
6	0.5348553340506115	1.6799390247047983
7	0.3781870606363102	1.3858321834830096
8	0.280420347387972	1.1743713484308336
9	0.21628248144445025	1.0189896906893563
>10	1.3061617361220648	12.864815043264736
>50	0.12941432382182907	4.744070745821447
>100	0.091050054842722	9.157563820654604
>500	0.006658771968029243	2.3528510394334687
>1k	0.0020844308128764076	1.6524507121359444
>5k	2.0845082418470955E-5	0.0743188838934491
>10k+	4.863852563955526E-5	0.7950832944709293
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	60515	0.21971978039713236	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	52902	0.19207825865602077	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	32902	0.11946162463234652	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	3.6308317011837127E-6	1.0892495103551139E-5	0.0	3.6308317011837127E-6	0.0
3	3.6308317011837127E-6	1.0892495103551139E-5	0.0	1.0892495103551139E-5	0.0
4	3.6308317011837127E-6	1.0892495103551139E-5	0.0	1.8154158505918564E-5	0.0
5	2.541582190828599E-5	3.267748531065342E-5	0.0	1.8154158505918564E-5	0.0
6	3.993914871302084E-5	3.267748531065342E-5	0.0	2.1784990207102278E-5	0.0
7	5.083164381657198E-5	3.267748531065342E-5	0.0	2.541582190828599E-5	3.6308317011837127E-6
8	7.624746572485797E-5	3.267748531065342E-5	0.0	4.3569980414204556E-5	3.6308317011837127E-6
9	8.713996082840911E-5	3.267748531065342E-5	0.0	7.987829742604168E-5	3.6308317011837127E-6
10	1.125557827366951E-4	3.267748531065342E-5	0.0	1.0166328763314397E-4	3.6308317011837127E-6
11	1.1981744613906252E-4	4.3569980414204556E-5	0.0	1.2344827784024624E-4	3.6308317011837127E-6
12	1.1981744613906252E-4	6.172413892012312E-5	0.0	1.4886409974853223E-4	3.6308317011837127E-6
13	2.0695740696747162E-4	6.172413892012312E-5	0.0	1.670182582544508E-4	3.6308317011837127E-6
14	5.482555868787407E-4	6.172413892012312E-5	0.0	1.9969574356510421E-4	3.6308317011837127E-6
15	5.954563989941289E-4	8.35091291272254E-5	0.0	2.214807337722065E-4	3.6308317011837127E-6
16	7.261663402367425E-4	8.35091291272254E-5	0.0	2.577890507840436E-4	3.6308317011837127E-6
17	7.3342800363911E-4	9.440162423077653E-5	0.0	2.940973677958807E-4	3.6308317011837127E-6
18	7.406896670414774E-4	9.803245593196025E-5	0.0	3.376673482100853E-4	3.6308317011837127E-6
19	7.515821621450285E-4	1.0892495103551139E-4	0.0	3.558215067160039E-4	3.6308317011837127E-6
20	7.661054889497634E-4	1.0892495103551139E-4	0.0	4.1391481393494324E-4	7.261663402367425E-6
21	7.806288157544982E-4	1.0892495103551139E-4	0.0	4.4659229924559667E-4	7.261663402367425E-6
22	7.951521425592331E-4	1.161866144378788E-4	0.0	4.756389528550664E-4	7.261663402367425E-6
23	8.024138059616005E-4	1.1981744613906252E-4	0.0	5.155781015680872E-4	1.0892495103551139E-5
24	8.024138059616005E-4	1.2707910954142996E-4	0.0	5.627789136834754E-4	1.0892495103551139E-5
25	8.060446376627842E-4	1.3434077294379736E-4	0.0	6.208722209024149E-4	1.0892495103551139E-5
26	8.35091291272254E-4	1.3434077294379736E-4	0.0	6.608113696154358E-4	1.8154158505918564E-5
27	8.677687765829073E-4	1.3434077294379736E-4	0.0	9.803245593196024E-4	1.8154158505918564E-5
28	9.004462618935608E-4	1.416024363461648E-4	0.0	0.0014850101657841385	2.541582190828599E-5
29	9.004462618935608E-4	1.5249493144971595E-4	0.0	0.002341886447263495	2.541582190828599E-5
30	9.004462618935608E-4	1.5612576315089967E-4	3.6308317011837127E-6	0.00341298179911269	2.90466536094697E-5
31	9.040770935947445E-4	1.5612576315089967E-4	3.6308317011837127E-6	0.0047055578847340915	2.90466536094697E-5
32	9.040770935947445E-4	1.5975659485208336E-4	3.6308317011837127E-6	0.00654275872553305	3.267748531065342E-5
33	9.040770935947445E-4	1.5975659485208336E-4	3.6308317011837127E-6	0.009843184741909046	4.3569980414204556E-5
34	9.040770935947445E-4	1.5975659485208336E-4	3.6308317011837127E-6	0.01491182579676151	4.3569980414204556E-5
35	9.077079252959282E-4	1.706490899556345E-4	3.6308317011837127E-6	0.022158965872324198	4.3569980414204556E-5
36	9.077079252959282E-4	1.706490899556345E-4	3.6308317011837127E-6	0.03414071048623045	4.3569980414204556E-5
37	9.077079252959282E-4	1.9243408016273678E-4	3.6308317011837127E-6	0.05714202931322927	4.3569980414204556E-5
38	9.11338756997112E-4	1.960649118639205E-4	3.6308317011837127E-6	0.0899284395749182	4.3569980414204556E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	29135	0.0	32.626194	1
TATGCCG	6215	0.0	28.671463	35
TCGTATG	6495	0.0	27.128231	32
TATCGTA	4900	0.0	26.844046	30
ATATCGT	4925	0.0	26.841522	29
CGTATGC	6690	0.0	26.633425	33
GACCGAT	6755	0.0	26.075441	24
ATCGTAT	5190	0.0	25.598192	31
ACCGATA	5200	0.0	25.415417	25
CGATATC	5490	0.0	24.314632	27
GATATCG	5625	0.0	23.695557	28
CCGATAT	5760	0.0	23.097475	26
AGACCGA	7870	0.0	22.937645	23
GCCGTCT	8080	0.0	22.490011	38
ATGCCGT	7920	0.0	22.359154	36
CCGTCTT	8205	0.0	22.281528	39
GCGGGCT	8440	0.0	21.879152	8
TGAGCGG	8620	0.0	21.855625	5
GTATCAA	43400	0.0	21.766714	2
TGCCGTC	8895	0.0	20.106495	37
>>END_MODULE