SRR522141_1.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	SRR522141_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	29311056
Sequences flagged as poor quality	0
Sequence length	50
%GC	47

Per base quality graph

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
TCTCTGAGCGGGCTGGCAAGGCAGACCGATATCGTATGCCGTCTTCTGCT	263859	0.9002029814278953	Illumina PCR Primer Index 1 (95% over 24bp)
TCTCTGAGCGGGCTGGCAAGGCAGACCGATCTCGTATGCCGTCTTCTGCT	53076	0.1810784299276014	Illumina Paired End PCR Primer 2 (96% over 29bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	44316	0.1511920962520081	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	40148	0.13697220598261625	No Hit

Adapter graph

Kmer graph

Sequence	Count	Obs/Exp Max	Max Obs/Exp Position
GCCGTCT	42145	35.155205	38
TATGCCG	42920	34.898087	35
ATGCCGT	43590	34.035404	36
CCGTCTT	44015	33.697426	39
TGCCGTC	44195	33.552387	37
CGTCTTC	45190	32.71877	40
TATCGTA	36520	32.698723	30
ACCGATA	36640	32.6143	25
ATCGTAT	37130	32.598972	31
TCGTATG	45880	32.581936	32
CCGATAT	36660	32.546566	26
ATATCGT	36665	32.535183	29
CGATATC	36830	32.423965	27
GATATCG	36910	32.407772	28
CGTATGC	46260	32.36295	33
GCGGGCT	48165	32.2378	8
AGACCGA	46875	32.075966	23
TGAGCGG	48735	32.045357	5
GACCGAT	46700	31.57782	24
CGGGCTG	50105	31.042149	9