##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR522141_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	29311056
Sequences flagged as poor quality	0
Sequence length	50
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	39.17256352005878	40.0	39.0	40.0	38.0	40.0
2	39.06375710243943	40.0	39.0	40.0	38.0	40.0
3	39.033456999979805	40.0	39.0	40.0	38.0	40.0
4	39.076811084527286	40.0	39.0	40.0	38.0	40.0
5	39.05727436091009	40.0	39.0	40.0	38.0	40.0
6	39.100127712901234	40.0	39.0	40.0	38.0	40.0
7	39.05961061245968	40.0	39.0	40.0	38.0	40.0
8	39.04401049215013	40.0	39.0	40.0	38.0	40.0
9	39.07447759644006	40.0	39.0	40.0	38.0	40.0
10	39.01375491213964	40.0	39.0	40.0	38.0	40.0
11	39.05279867774126	40.0	39.0	40.0	38.0	40.0
12	38.988400076749194	40.0	39.0	40.0	38.0	40.0
13	38.984128207458646	40.0	39.0	40.0	38.0	40.0
14	38.954025777849836	40.0	39.0	40.0	38.0	40.0
15	38.91983557330722	40.0	39.0	40.0	37.0	40.0
16	38.8853357927466	40.0	39.0	40.0	37.0	40.0
17	38.82592602600193	40.0	39.0	40.0	37.0	40.0
18	38.80063468201214	40.0	39.0	40.0	36.0	40.0
19	38.76818180825693	40.0	39.0	40.0	36.0	40.0
20	38.73152891523253	40.0	39.0	40.0	36.0	40.0
21	38.66062822847461	40.0	39.0	40.0	36.0	40.0
22	38.587242165550094	40.0	39.0	40.0	36.0	40.0
23	38.54523439892442	40.0	38.0	40.0	36.0	40.0
24	38.4801138519199	40.0	38.0	40.0	35.0	40.0
25	38.404144224622954	40.0	38.0	40.0	35.0	40.0
26	38.245362568991034	40.0	38.0	40.0	35.0	40.0
27	38.15130700169929	40.0	38.0	40.0	35.0	40.0
28	38.283392587425034	40.0	38.0	40.0	35.0	40.0
29	38.18892778888621	40.0	38.0	40.0	35.0	40.0
30	38.10518652074494	40.0	38.0	40.0	35.0	40.0
31	38.153882719203295	40.0	39.0	40.0	35.0	40.0
32	38.195541266066975	40.0	39.0	40.0	35.0	40.0
33	38.183001560912714	40.0	39.0	40.0	35.0	40.0
34	38.171336986289404	40.0	39.0	40.0	35.0	40.0
35	38.06257976512344	40.0	39.0	40.0	35.0	40.0
36	38.097788936707026	40.0	39.0	40.0	35.0	40.0
37	38.03441015567641	40.0	39.0	40.0	35.0	40.0
38	37.950458386760275	40.0	39.0	40.0	35.0	40.0
39	37.89259134846591	40.0	38.0	40.0	35.0	40.0
40	37.83515288565516	40.0	38.0	40.0	35.0	40.0
41	37.735749063425075	40.0	38.0	40.0	35.0	40.0
42	37.62586097205096	40.0	38.0	40.0	35.0	40.0
43	37.554704102097176	40.0	38.0	40.0	34.0	40.0
44	37.468491991554316	40.0	38.0	40.0	34.0	40.0
45	37.37159926957255	40.0	38.0	40.0	34.0	40.0
46	37.15232842515125	40.0	38.0	40.0	33.0	40.0
47	37.080392463512744	40.0	38.0	40.0	33.0	40.0
48	36.955834856308144	40.0	38.0	40.0	33.0	40.0
49	36.85426335373246	40.0	38.0	40.0	33.0	40.0
50	36.80729435336618	40.0	38.0	40.0	33.0	40.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1	1	-0.20270361341614063
1	2	-0.2122982744643096
1	3	-0.2331580615377078
1	4	-0.213598965712265
1	5	-0.21157226298287668
1	6	-0.22315227778013025
1	7	-0.2340432134463697
1	8	-0.260886853417027
1	9	-0.24917502177229522
1	10	-0.27318913872345263
1	11	-0.2596377939070962
1	12	-0.2795550154805042
1	13	-0.26209032578487523
1	14	-0.2803927555861989
1	15	-0.2707068554547192
1	16	-0.2816219436832341
1	17	-0.2881108869540725
1	18	-0.3014420595838203
1	19	-0.3350486451271877
1	20	-0.34193493971114464
1	21	-0.3063017222538349
1	22	-0.3256596489154191
1	23	-0.3361509254685373
1	24	-0.33670497157484647
1	25	-0.34342652433243614
1	26	-0.3330222456770926
1	27	-0.3367010242533297
1	28	-0.24411267128081704
1	29	-0.25230351587909894
1	30	-0.24339961217817319
1	31	-0.1645131342862669
1	32	-0.2551958382578974
1	33	-0.231181850601601
1	34	-0.26846466067921426
1	35	-0.255430207015074
1	36	-0.24079656349716316
1	37	-0.2897470272131031
1	38	-0.300519425571963
1	39	-0.30345840748103825
1	40	-0.28867388276493244
1	41	-0.31001545900039673
1	42	-0.2907213788027363
1	43	-0.3391977538171673
1	44	-0.3251427633056778
1	45	-0.34598694512570205
1	46	-0.40577051408458686
1	47	-0.3954305519448411
1	48	-0.46680606862481966
1	49	-0.4534966651142156
1	50	-0.4631613606504885
2	1	-0.16816295300728257
2	2	-0.17849847019310516
2	3	-0.19301907092223303
2	4	-0.19395926089695337
2	5	-0.18096905055823242
2	6	-0.19509180403911586
2	7	-0.21026275420362595
2	8	-0.233602438191582
2	9	-0.23258546430282934
2	10	-0.2687634224416229
2	11	-0.24784268340785331
2	12	-0.2321254045577703
2	13	-0.24445174494094601
2	14	-0.23060176543296507
2	15	-0.2691999705022994
2	16	-0.29644216411026036
2	17	-0.28700200178439417
2	18	-0.29206771791125163
2	19	-0.3419020199940377
2	20	-0.2786167501874246
2	21	-0.32626688572751306
2	22	-0.2516303041267207
2	23	-0.2965241971417072
2	24	-0.32025987681933543
2	25	-0.2947335346175919
2	26	-0.2940759742794725
2	27	-0.3188066890581709
2	28	-0.2432583001851043
2	29	-0.23817896781780945
2	30	-0.21514181116897646
2	31	-0.1557778651013635
2	32	-0.2833488823331365
2	33	-0.21105733142537275
2	34	-0.2528333327328056
2	35	-0.24979737850224382
2	36	-0.2722365198636538
2	37	-0.22691569784358023
2	38	-0.2988781691975646
2	39	-0.26731602981929825
2	40	-0.25433317026853075
2	41	-0.2708021330817232
2	42	-0.319354842527126
2	43	-0.31485445022818226
2	44	-0.335657413725869
2	45	-0.36043973914812
2	46	-0.4043786114094914
2	47	-0.37321434198781844
2	48	-0.3635256612074329
2	49	-0.4491620796237612
2	50	-0.4710278041353604
3	1	-0.1838362854468798
3	2	-0.203429633976846
3	3	-0.20206391255155864
3	4	-0.19736361209309905
3	5	-0.19271528538644844
3	6	-0.1833455122140748
3	7	-0.23123439165851778
3	8	-0.19940657370676718
3	9	-0.22429130436331945
3	10	-0.21052990000334404
3	11	-0.21185855581038737
3	12	-0.22163465374603675
3	13	-0.2185361356475397
3	14	-0.21834407823504165
3	15	-0.23942377022327577
3	16	-0.24266562888813326
3	17	-0.2539213261001265
3	18	-0.2748490973620079
3	19	-0.2556119845309226
3	20	-0.27041278153416926
3	21	-0.31016811532816035
3	22	-0.31565805107509703
3	23	-0.3178048069711039
3	24	-0.34855519292712245
3	25	-0.30665307325998725
3	26	-0.3168927675946449
3	27	-0.3521776495914679
3	28	-0.28270025191660864
3	29	-0.31998837848863104
3	30	-0.2993338280580744
3	31	-0.28658158128716593
3	32	-0.25180053789245704
3	33	-0.28140513704033765
3	34	-0.22151930473339831
3	35	-0.22590195613501862
3	36	-0.24505457485318516
3	37	-0.2971681861748863
3	38	-0.27092750300096924
3	39	-0.28609133164864176
3	40	-0.29568838659259455
3	41	-0.2882162973599307
3	42	-0.3275813881198957
3	43	-0.40792439105538136
3	44	-0.35991106487922053
3	45	-0.36012827997720365
3	46	-0.3464282331088384
3	47	-0.3938639560334849
3	48	-0.46608491484733605
3	49	-0.45120261937406525
3	50	-0.4359216581666914
4	1	-0.17816466311686696
4	2	-0.1750938925602341
4	3	-0.18789462358483888
4	4	-0.16382952502853243
4	5	-0.16572149162087157
4	6	-0.17800260898381737
4	7	-0.21616103901158112
4	8	-0.1902958533527439
4	9	-0.2219253690898455
4	10	-0.21707924440408277
4	11	-0.20024042780179485
4	12	-0.23223187680869728
4	13	-0.23462432382658704
4	14	-0.24095597865012763
4	15	-0.24656521194802394
4	16	-0.2547553672671725
4	17	-0.24510753363995974
4	18	-0.28692352334716276
4	19	-0.280091551387855
4	20	-0.3162104411502682
4	21	-0.28782799791213876
4	22	-0.2796170878083757
4	23	-0.2956117466048198
4	24	-0.30925000977836703
4	25	-0.3365197422628583
4	26	-0.3257357659529916
4	27	-0.32258310821119807
4	28	-0.2240016391443831
4	29	-0.28367340542458663
4	30	-0.26750165234950174
4	31	-0.25254960536052096
4	32	-0.18266449376761784
4	33	-0.23146360911425745
4	34	-0.26732140658479153
4	35	-0.24827655988974584
4	36	-0.26772189527785883
4	37	-0.28287898967659686
4	38	-0.26016351117955594
4	39	-0.24762119027074903
4	40	-0.268000128425939
4	41	-0.25997963457965767
4	42	-0.24342613890156173
4	43	-0.29638465227837685
4	44	-0.26697720636065014
4	45	-0.24274717094822762
4	46	-0.3280402647522749
4	47	-0.3008488484128833
4	48	-0.3246346212164042
4	49	-0.3200326744044446
4	50	-0.33331664946497597
5	1	-0.16860806656976024
5	2	-0.19301993824685582
5	3	-0.19042176196889926
5	4	-0.21142631848596238
5	5	-0.19027487394086506
5	6	-0.2190335105237864
5	7	-0.21820252836221243
5	8	-0.24105757823622298
5	9	-0.23532409737492088
5	10	-0.24570229564226764
5	11	-0.23766973106041434
5	12	-0.24492760655134305
5	13	-0.25572237297603095
5	14	-0.2523908454628483
5	15	-0.274053704578904
5	16	-0.271483646479453
5	17	-0.2524271383375307
5	18	-0.2695894391368583
5	19	-0.2850264455941982
5	20	-0.2892914113581071
5	21	-0.26840283977390555
5	22	-0.2804954130115007
5	23	-0.30370105680901105
5	24	-0.3269522129997142
5	25	-0.3405404821176816
5	26	-0.27407801828545075
5	27	-0.3579513642259613
5	28	-0.2803488260251541
5	29	-0.23883353648835737
5	30	-0.2590934747726621
5	31	-0.1950562044200197
5	32	-0.3026795513633189
5	33	-0.2958280903493886
5	34	-0.26387784675554826
5	35	-0.3240256030722506
5	36	-0.3039152555001934
5	37	-0.31049014373445516
5	38	-0.3333609909585249
5	39	-0.34621469092748214
5	40	-0.3328877965837691
5	41	-0.33225889089784744
5	42	-0.40298610493282894
5	43	-0.37169674878145287
5	44	-0.3592383425893715
5	45	-0.45016008093024595
5	46	-0.4057904106310062
5	47	-0.3677640535791582
5	48	-0.4501412326075993
5	49	-0.44726611549948103
5	50	-0.44724039946405014
6	1	-0.17801621140770152
6	2	-0.201994464326674
6	3	-0.1885388228252296
6	4	-0.20349187411430592
6	5	-0.1848470980728223
6	6	-0.21514510150752386
6	7	-0.22975798876866804
6	8	-0.2190011240544507
6	9	-0.24188958783213366
6	10	-0.2629151729172605
6	11	-0.2495083876031572
6	12	-0.25188774815841697
6	13	-0.2708475229315326
6	14	-0.2419963316338496
6	15	-0.26828730897461384
6	16	-0.24956264469450673
6	17	-0.27451265501628086
6	18	-0.2469867630909519
6	19	-0.27288473766936505
6	20	-0.2924605967369942
6	21	-0.2735177266834228
6	22	-0.26774078130267753
6	23	-0.3310903103485714
6	24	-0.3304624718810558
6	25	-0.3266448266120463
6	26	-0.2950088466161347
6	27	-0.31709633701053974
6	28	-0.26549244225972046
6	29	-0.3125230846374407
6	30	-0.2096936682374988
6	31	-0.23835765905314332
6	32	-0.2841761731575758
6	33	-0.2694969503228606
6	34	-0.2993227246986905
6	35	-0.24600837673303033
6	36	-0.23642698236405124
6	37	-0.3014536881974834
6	38	-0.29775110286602313
6	39	-0.3449397455237815
6	40	-0.31870601843169766
6	41	-0.3160753347783043
6	42	-0.3582674089677411
6	43	-0.3780773063086542
6	44	-0.40324069640399784
6	45	-0.424816232871045
6	46	-0.40668079008906943
6	47	-0.439292689143322
6	48	-0.48430603249845916
6	49	-0.48736304151572796
6	50	-0.46628029702718976
7	1	-0.20936034176919804
7	2	-0.24236608624359235
7	3	-0.2560030317889641
7	4	-0.2391783844003541
7	5	-0.22318896888205586
7	6	-0.23761331844336553
7	7	-0.24964839584443155
7	8	-0.2825630568047046
7	9	-0.28027046930091615
7	10	-0.27928955099577024
7	11	-0.2807444418974967
7	12	-0.26258750741114056
7	13	-0.30097234493951674
7	14	-0.30980601555607024
7	15	-0.3049741784384352
7	16	-0.27347685136547994
7	17	-0.30013668773413116
7	18	-0.32557389140213644
7	19	-0.2969421851350802
7	20	-0.2948493104596679
7	21	-0.2777310123881591
7	22	-0.2968911436607371
7	23	-0.26868257405470786
7	24	-0.3050738612992845
7	25	-0.2691346291772305
7	26	-0.2743130260818418
7	27	-0.29701776166207594
7	28	-0.23412175345196573
7	29	-0.2713297376694257
7	30	-0.27936659861845925
7	31	-0.2133720375942474
7	32	-0.25726461385942656
7	33	-0.24048829576567954
7	34	-0.23563089088974465
7	35	-0.25991251002066207
7	36	-0.26941122789372685
7	37	-0.2854540464339479
7	38	-0.2726649627307509
7	39	-0.2944622918965152
7	40	-0.33389779932546304
7	41	-0.282452261122927
7	42	-0.287378451378018
7	43	-0.28970548160622656
7	44	-0.3323751371416108
7	45	-0.2993768721241068
7	46	-0.35020218994375796
7	47	-0.3630789580635252
7	48	-0.39059394299959393
7	49	-0.3980866831991179
7	50	-0.35147739902332376
8	1	-0.1937048478032466
8	2	-0.2131845693054828
8	3	-0.21534644690549953
8	4	-0.21184531810357043
8	5	-0.18310387430627628
8	6	-0.22056361684316528
8	7	-0.2506908615768353
8	8	-0.23916422127263104
8	9	-0.2493524524294628
8	10	-0.22946943953259336
8	11	-0.23201882917050654
8	12	-0.2115121034932912
8	13	-0.25559962582234164
8	14	-0.23454456400679646
8	15	-0.22897469485621968
8	16	-0.2769965876433176
8	17	-0.31815777238602294
8	18	-0.2805604076616177
8	19	-0.2718012160211316
8	20	-0.2951355070276591
8	21	-0.2968397342812352
8	22	-0.28921984177331694
8	23	-0.2860998170518485
8	24	-0.2766530674784775
8	25	-0.27815301106216594
8	26	-0.27556527901460726
8	27	-0.28349784782800924
8	28	-0.27410046476087757
8	29	-0.2085255409624054
8	30	-0.2747762283655746
8	31	-0.20966986981323998
8	32	-0.20923697768358096
8	33	-0.2462581509911459
8	34	-0.2631041664199998
8	35	-0.24688842948356182
8	36	-0.25106097204723454
8	37	-0.28621516077684817
8	38	-0.3229272794904645
8	39	-0.31276788783691245
8	40	-0.32401504963550565
8	41	-0.3516640408653231
8	42	-0.3470906116625443
8	43	-0.38744380645648846
8	44	-0.3604826495457232
8	45	-0.3790809009351648
8	46	-0.3603887112590769
8	47	-0.35751588061079076
8	48	-0.476151519218611
8	49	-0.41088189676465703
8	50	-0.4370610970742064
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113	30	-0.07469115511351276
113	31	-0.1254556949904142
113	32	-0.14794540412218282
113	33	-0.04064067221165857
113	34	-0.05258752971717939
113	35	-0.11380469907153667
113	36	-0.08540185960843871
113	37	-0.09455390507567785
113	38	-0.08006428150879685
113	39	-0.04177822960393485
113	40	-0.04065764983312192
113	41	0.009631972768033847
113	42	-0.013283813579789694
113	43	-0.08376389713180288
113	44	-0.10249006374576908
113	45	-0.07410496555350221
113	46	0.054345071931912514
113	47	0.033939270984767234
113	48	0.05301709569431523
113	49	0.04913011809096446
113	50	0.04703309685637436
114	1	-0.03454569742063285
114	2	-0.054329144304276156
114	3	-0.04503189325694734
114	4	-0.05359933275452988
114	5	-0.0511555518271507
114	6	-0.0706688441054979
114	7	-0.07308196818534185
114	8	-0.07394581368861708
114	9	-0.060589522194980816
114	10	-0.09518617870052992
114	11	-0.09513749733069687
114	12	-0.08830966052889977
114	13	-0.1144467575508159
114	14	-0.11923595462570091
114	15	-0.09953806388637076
114	16	-0.10906187456206595
114	17	-0.09617319612602415
114	18	-0.12847781159037197
114	19	-0.12342016963267355
114	20	-0.11927146339763084
114	21	-0.13748142468200086
114	22	-0.08872072664615871
114	23	-0.1297129272546016
114	24	-0.16122814151423626
114	25	-0.07635835634440014
114	26	-0.14995069138785766
114	27	-0.12733975538436937
114	28	-0.0653668590276979
114	29	-0.12485222062225887
114	30	-0.07477592723380155
114	31	-0.05437946003920757
114	32	-0.08427556889403576
114	33	-0.05277538429693607
114	34	-0.07681555923495154
114	35	-0.045026422809307576
114	36	-0.04234464112170144
114	37	-0.031011559402486455
114	38	-0.07937220854623916
114	39	-0.03709448983043018
114	40	-0.0252750084460871
114	41	-0.014053065651339125
114	42	0.016080219454948974
114	43	-0.036449522448045
114	44	0.042578098552802146
114	45	0.017674640655911844
114	46	0.07619794607918351
114	47	0.10151128696525547
114	48	0.10637482078152516
114	49	0.041332614952317215
114	50	0.10671357810473836
115	1	-0.012511383105355378
115	2	-0.003362621468802729
115	3	-0.007119501526126726
115	4	-7.607584691200486E-4
115	5	-0.009080355172713439
115	6	-0.0268214968348488
115	7	-0.031094463388505744
115	8	-0.031426534524804595
115	9	-0.021830506360046797
115	10	-0.021023969027829992
115	11	-0.08715211770626041
115	12	-0.04961401991860015
115	13	-0.07059553481678904
115	14	-0.09510521726180343
115	15	-0.05260658233218862
115	16	-0.08028227824350154
115	17	-0.09565659432482221
115	18	-0.10540814063747206
115	19	-0.08225763193873092
115	20	-0.06524058887272588
115	21	-0.12744050434810816
115	22	-0.10074935441549826
115	23	-0.12263863614144555
115	24	-0.12609324119343768
115	25	-0.10877901383069144
115	26	-0.10379559433029328
115	27	-0.12180346597590841
115	28	-0.0958665784998729
115	29	-0.09609843539638518
115	30	-0.1118674552935488
115	31	-0.12523146673134988
115	32	-0.09944907186965679
115	33	-0.11068564832238081
115	34	-0.013110616246059692
115	35	-0.05372896579719111
115	36	-0.06355715456233924
115	37	-0.09861663701940415
115	38	-0.06037656147683634
115	39	-0.08280998775781967
115	40	-0.057461567680519465
115	41	-0.0010838654532605574
115	42	0.026141023702251687
115	43	0.015956950335692
115	44	0.003221172175202014
115	45	-0.015889506495184946
115	46	0.030801198682141262
115	47	0.04525706163365584
115	48	0.04108628240114598
115	49	0.05521500603722984
115	50	0.03386216159754696
116	1	-0.03844477110985167
116	2	-0.03828676358853045
116	3	-0.055462565934476515
116	4	-0.030665171191486706
116	5	-0.049803912577111475
116	6	-0.053997224931094934
116	7	-0.05022822863714538
116	8	-0.06687851737996198
116	9	-0.10123847390814689
116	10	-0.07177902671070768
116	11	-0.05806120147553884
116	12	-0.08155661126646407
116	13	-0.07294328401619765
116	14	-0.06382466189349856
116	15	-0.09097003389355507
116	16	-0.08929044161298805
116	17	-0.09445607571229431
116	18	-0.09776274227643
116	19	-0.09085972772496831
116	20	-0.09740749780727498
116	21	-0.11117171331609654
116	22	-0.09470696877171747
116	23	-0.08519462881908879
116	24	-0.05665271310400044
116	25	-0.1138281557049865
116	26	-0.06281085884296544
116	27	-0.10059301230776185
116	28	-0.032914544922263644
116	29	-0.0028585273643670916
116	30	0.0017100221055059706
116	31	0.05570123733816246
116	32	0.062180237984307496
116	33	0.054863047521585884
116	34	0.036966030286521345
116	35	0.07666005249758001
116	36	0.041724027800910335
116	37	0.01554312103122868
116	38	-0.008016619254618718
116	39	-0.024263595040387997
116	40	-0.02496028325715116
116	41	0.03682873878138082
116	42	0.08118100935597994
116	43	0.039964076349242816
116	44	-0.00349136504034675
116	45	0.036303948279694964
116	46	-0.010818634254469828
116	47	0.01828723396563703
116	48	-0.012682644436139867
116	49	-0.11137853303508649
116	50	-0.028673031602636456
117	1	-0.04803302905900608
117	2	-0.0676794004294976
117	3	-0.05909009821640154
117	4	-0.07950428269612786
117	5	-0.09197249491986526
117	6	-0.07814088276069242
117	7	-0.08442675212624096
117	8	-0.10917714185021765
117	9	-0.06027000391778614
117	10	-0.11115135622692662
117	11	-0.07085820305136536
117	12	-0.0931767900380649
117	13	-0.08409137267910438
117	14	-0.1146446739816156
117	15	-0.07392509227825883
117	16	-0.09700554636363279
117	17	-0.15914328991938476
117	18	-0.0837437297215331
117	19	-0.09421769219626697
117	20	-0.07545231688470722
117	21	-0.10772377893322016
117	22	-0.07782434692032325
117	23	-0.11438021964143985
117	24	-0.10491185292588767
117	25	-0.08788183781674519
117	26	-0.11656683653129818
117	27	-0.09132469984893987
117	28	-0.018273005970598888
117	29	0.04322984850989542
117	30	0.01754323239992317
117	31	-0.05925170803293156
117	32	-0.024210577123682242
117	33	-0.04967363259422086
117	34	-0.011333463032265456
117	35	-0.07821437703267975
117	36	-0.02329603856411211
117	37	-0.02997765049098433
117	38	-5.973330546211741E-4
117	39	-0.0379417363491541
117	40	-0.02737780674156909
117	41	0.004593199052166597
117	42	-0.06560500138531467
117	43	-0.0724955635411817
117	44	-0.010623354986954325
117	45	-0.0268945760738859
117	46	0.022541696460727678
117	47	-0.05958160386668254
117	48	-0.1043379077185449
117	49	-0.11221076084604675
117	50	-0.028916125454856
118	1	-0.055975236056092115
118	2	-0.06697908484942161
118	3	-0.06440737661241513
118	4	-0.07427655885512507
118	5	-0.05787903992853671
118	6	-0.09940020890623202
118	7	-0.0796999330505983
118	8	-0.0858068202231479
118	9	-0.09412063758269795
118	10	-0.1170496997274384
118	11	-0.12674787263111398
118	12	-0.13563179607267983
118	13	-0.12945596879924182
118	14	-0.11848786088484076
118	15	-0.15931901428375994
118	16	-0.1468546450801398
118	17	-0.16177635569231086
118	18	-0.14729578652629982
118	19	-0.14349090112568064
118	20	-0.11587545271346755
118	21	-0.11620969107801926
118	22	-0.1471021377053816
118	23	-0.1299248956114738
118	24	-0.13679135406228227
118	25	-0.14386946549998925
118	26	-0.18304367069495697
118	27	-0.16590402743955934
118	28	-0.12210089613234487
118	29	-0.10720806522208903
118	30	-0.14266876042323418
118	31	-0.1641433263391221
118	32	-0.14590634197232077
118	33	-0.0789673345882278
118	34	-0.13396243528134022
118	35	-0.12807542971763297
118	36	-0.0703841041739608
118	37	-0.11676025983586413
118	38	-0.081453408640634
118	39	-0.07350045942447281
118	40	-0.08785646071172692
118	41	-0.06588851389844308
118	42	-0.07902455122775365
118	43	-0.14512472244417296
118	44	-0.12112406475192472
118	45	-0.1057152792355609
118	46	-0.0833967160469129
118	47	-0.04783286719622737
118	48	-0.06730330335317802
118	49	-0.044236610952715694
118	50	-0.10229086113297114
119	1	-0.0442408809320014
119	2	-0.031035780328117824
119	3	-0.04226394002361644
119	4	-0.025915575719835715
119	5	-0.028731911056752324
119	6	-0.036073702319910694
119	7	-0.05421889822619619
119	8	-0.06848642054175968
119	9	-0.03954996748947792
119	10	-0.05209845757900666
119	11	-0.06775146907236262
119	12	-0.07938000422932845
119	13	-0.07041331109128635
119	14	-0.05479975411311955
119	15	-0.12517612036059234
119	16	-0.10076788580395402
119	17	-0.09525404224081058
119	18	-0.09510712370799013
119	19	-0.08472186812433335
119	20	-0.08073956181390685
119	21	-0.0650217025672788
119	22	-0.09300131481131757
119	23	-0.07518114563642797
119	24	-0.11431239933351378
119	25	-0.08969613116379804
119	26	-0.11167559782211356
119	27	-0.09238894422188793
119	28	-0.010507907061729327
119	29	-0.02264712327815488
119	30	-0.03332611805303998
119	31	-0.004401634763823381
119	32	-0.009101126585385089
119	33	0.024198433118513663
119	34	-0.012632432624585022
119	35	0.02227985385250264
119	36	0.019500301940681197
119	37	0.001096506762976901
119	38	0.0584735933009668
119	39	0.09727748085204979
119	40	0.07583083345854646
119	41	0.08164029003868478
119	42	0.06496721165338215
119	43	0.06355603777910801
119	44	0.10337872918263002
119	45	0.04467782547552446
119	46	0.10266954732782096
119	47	0.09512411026287282
119	48	0.09195961707317934
119	49	0.11394795827333581
119	50	0.07558642423650497
120	1	-0.07897957734494554
120	2	-0.06151413610526646
120	3	-0.062395268538836035
120	4	-0.07847286139679
120	5	-0.08799087198287481
120	6	-0.07575319671140335
120	7	-0.07950883060655656
120	8	-0.06205567474411566
120	9	-0.08367930647939659
120	10	-0.08537058316804291
120	11	-0.1012569049533667
120	12	-0.12364630001601995
120	13	-0.12894369629659508
120	14	-0.13303550892083393
120	15	-0.09469000143647577
120	16	-0.17375731464500888
120	17	-0.1480264852871116
120	18	-0.1310086286348877
120	19	-0.13510887267467808
120	20	-0.15332497868395478
120	21	-0.2007647853041803
120	22	-0.1717882311653085
120	23	-0.15938419950084892
120	24	-0.14247088018488085
120	25	-0.117769698947086
120	26	-0.21966475159678112
120	27	-0.16369032636848857
120	28	-0.07503090180949101
120	29	-0.07373465699203052
120	30	-0.15021400522357453
120	31	-0.04662936025953002
120	32	-0.06393911393991658
120	33	-0.017967486345256134
120	34	-0.057578084029231036
120	35	-0.04951653835627212
120	36	-0.0013548962523159958
120	37	-0.03172024896829129
120	38	-0.07159467277826792
120	39	0.0031785815699834075
120	40	-0.011187703681471817
120	41	0.04773201687338968
120	42	0.0019515734358748205
120	43	-0.03668140215682314
120	44	0.034905474610219756
120	45	-0.014919189857124593
120	46	0.05300973951824517
120	47	0.01799806229275447
120	48	0.03739880470946133
120	49	0.04026574152715057
120	50	0.015415103840481947
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	8093.0
3	446.0
4	649.0
5	772.0
6	1228.0
7	2086.0
8	2651.0
9	3159.0
10	4547.0
11	6845.0
12	7732.0
13	10428.0
14	13994.0
15	16752.0
16	21506.0
17	23892.0
18	31414.0
19	47791.0
20	43785.0
21	32999.0
22	40071.0
23	52459.0
24	66136.0
25	61182.0
26	86904.0
27	114247.0
28	138338.0
29	140068.0
30	171788.0
31	213652.0
32	266497.0
33	319016.0
34	453780.0
35	678438.0
36	1068477.0
37	1954995.0
38	4034387.0
39	1.8820569E7
40	349283.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.729504753060404	18.998666655599017	11.511568751610403	24.76025983973018
2	18.03963818816233	21.133938245417426	35.28874863932992	25.537674927090325
3	18.32197000430522	24.474528061825936	31.83070528840573	25.372796645463108
4	12.095028204570253	15.654619720210828	37.57170212529313	34.67864994992579
5	13.307487113395029	37.93847277286769	36.03579140922115	12.718248704516139
6	32.827288788230625	37.10079227442368	17.11751360988154	12.95440532746415
7	28.12568404222625	32.81878346518802	21.941215628669262	17.114316863916468
8	29.082783643141347	33.092472001008765	20.085366422826937	17.73937793302295
9	27.057418197420112	12.587530111504682	19.145280879678985	41.20977081139622
10	16.54409858177747	26.593767211935322	35.035445328206535	21.82668887808068
11	36.44959089839684	21.22931360780724	22.714214049469934	19.606881444325992
12	21.524447976217576	25.476571024940213	31.714091092453305	21.28488990638891
13	29.55202637530357	19.688011240536678	25.65711040912344	25.102851975036316
14	21.064061970336383	20.111400967607583	28.324605568629117	30.499931493426917
15	24.983303228652016	28.26657968242427	23.235010707222557	23.515106381701155
16	24.414016335678934	26.98314247019964	24.99096927794072	23.611871916180707
17	22.095638587705608	27.012489758130858	26.47936328189609	24.412508372267446
18	22.65605742568684	27.136458504806654	27.448284214844804	22.7591998546617
19	22.977510602142754	27.085022115886918	27.49149331228462	22.445973969685703
20	24.542971089134422	25.571845654417906	27.239520131925648	22.645663124522024
21	24.89048842184328	25.73979593229258	26.77197982904471	22.597735816819426
22	23.616181511216254	25.42870073436479	26.96492520649265	23.990192547926306
23	23.08556902620792	26.523800403011883	26.971042468276174	23.419588102504026
24	23.748850945527174	25.843899994595898	27.32565827720434	23.08159078267259
25	22.978772926631873	26.934698784785056	27.17990809825739	22.906620190325686
26	22.829483708297047	25.818707385681066	27.098920154639593	24.25288875138229
27	22.93890633574228	25.67616989165576	27.105689475504736	24.279234297097226
28	23.796980953116353	25.7118282207423	27.370539187883203	23.120651638258142
29	22.471081862084237	26.974635740533138	27.39782800271505	23.15645439466758
30	22.54863242819572	26.35562405072614	28.440524577104597	22.655218943973544
31	23.139149896286177	26.921131175457298	27.018971176130542	22.920747752125983
32	22.519935082884462	26.082702647272765	28.17312248505391	23.224239784788864
33	22.581870782802373	25.814244759471066	27.332858596092503	24.27102586163406
34	24.23925838235253	25.356124108342453	27.468342337645733	22.936275171659283
35	22.844640625911342	25.6504643758535	28.907393457252695	22.597501540982467
36	22.76979019599719	27.491416112813184	26.85120040872555	22.887593282464078
37	23.15443207307724	25.886533627615893	28.285610024694595	22.673424274612273
38	24.29352232444674	26.018969374631013	27.02851575654284	22.65899254437941
39	23.0038190856436	26.126651505985443	26.814559022952132	24.05497038541883
40	22.87337047006271	25.616484787169803	27.529142873593237	23.981001869174253
41	23.783994054581612	26.133064315170788	27.30362168854736	22.77931994170024
42	22.678580395819857	26.019820985564873	28.563803778241475	22.737794840373795
43	22.77592032444555	25.56369889441963	27.33555774918919	24.324823031945638
44	22.623272227943794	26.08270615566447	28.3842620028599	22.909759613531836
45	22.61947644741347	25.891644057314494	28.395961324701428	23.092918170570606
46	22.643408687392636	26.21281510860442	27.169480404947716	23.97429579905523
47	22.72205149269839	25.98877829601829	28.51995590039759	22.769214310885726
48	24.166220786256886	26.262357851284207	26.731037424006075	22.840383938452828
49	22.547000246928267	26.413859873044977	27.097427163879615	23.94171271614714
50	22.70153705690145	26.173184101512774	28.156071713416296	22.96920712816948
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	6826.0
1	5701.0
2	4576.0
3	11490.0
4	18404.0
5	19470.5
6	20537.0
7	16578.5
8	12620.0
9	17287.5
10	21955.0
11	32891.0
12	43827.0
13	72315.5
14	100804.0
15	108476.5
16	116149.0
17	151479.0
18	186809.0
19	196816.5
20	206824.0
21	241100.5
22	275377.0
23	319838.5
24	364300.0
25	415534.5
26	466769.0
27	537219.5
28	607670.0
29	679856.0
30	752042.0
31	832496.0
32	912950.0
33	988190.0
34	1063430.0
35	1141970.0
36	1220510.0
37	1295273.0
38	1370036.0
39	1421258.5
40	1472481.0
41	1534004.5
42	1595528.0
43	1650190.5
44	1704853.0
45	1704659.0
46	1704465.0
47	1717966.5
48	1731468.0
49	1757941.0
50	1784414.0
51	1740706.0
52	1696998.0
53	1662589.0
54	1628180.0
55	1585131.5
56	1542083.0
57	1604681.0
58	1667279.0
59	1493556.0
60	1319833.0
61	1162226.5
62	1004620.0
63	885843.5
64	767067.0
65	670805.5
66	574544.0
67	502280.5
68	430017.0
69	378069.5
70	326122.0
71	260465.0
72	194808.0
73	171694.0
74	148580.0
75	114939.0
76	81298.0
77	66921.0
78	52544.0
79	42147.0
80	31750.0
81	26426.5
82	21103.0
83	19441.0
84	17779.0
85	16312.0
86	14845.0
87	12163.0
88	9481.0
89	8140.5
90	6800.0
91	6342.0
92	5884.0
93	4211.0
94	2538.0
95	1734.0
96	930.0
97	591.0
98	252.0
99	174.5
100	97.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.12469356272936738
2	4.435186504368863E-4
3	0.00835862071977209
4	0.0019037185149521736
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	4.4693033236332393E-4
19	0.0
20	0.0
21	0.0
22	0.0023574722111683725
23	0.0025724081725339407
24	0.0
25	0.12165716581483792
26	0.10523332902096737
27	0.16450447912896757
28	0.19081195846372784
29	0.18713075366510165
30	0.26449746471092683
31	0.27176775889616533
32	0.32316815879987404
33	0.31895814330265004
34	0.2914599869755631
35	0.32727582383930487
36	0.36875505270093306
37	0.38333999293645377
38	0.46085340630511573
39	0.41756939770440205
40	0.4516930403326308
41	0.4650873035758248
42	0.4688708588322441
43	0.54597828205166
44	0.582902915541494
45	0.6469435969826539
46	0.5783346734419941
47	0.563661029476386
48	0.5488133897325296
49	0.5489055051445434
50	0.6180773562030655
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2.9311056E7
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.68660732097945
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.34372801472479	44.11127757205553
2	7.49003495072071	7.74268990636626
3	2.52532067446557	3.9157577418195864
4	1.2683835645996828	2.622337729433919
5	0.7366319977628002	1.9037004404217233
6	0.4881995746629505	1.5140027825923865
7	0.32234464079927744	1.1662630609710098
8	0.2657395002669975	1.0988138559978888
9	0.18896975993116294	0.8790485199391598
>10	1.1703762474449597	11.269859522495793
>50	0.10631710187894045	3.840335628913855
>100	0.08030191554193446	8.111925718472515
>500	0.007681325301927464	2.7308532679996955
>1k	0.005407690434924273	5.377062469979911
>5k	4.1731308484206436E-4	1.4622510182664048
>10k+	1.4572837880594058E-4	2.253820764274489
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCTCTGAGCGGGCTGGCAAGGCAGACCGATATCGTATGCCGTCTTCTGCT	263859	0.9002029814278953	Illumina PCR Primer Index 1 (95% over 24bp)
TCTCTGAGCGGGCTGGCAAGGCAGACCGATCTCGTATGCCGTCTTCTGCT	53076	0.1810784299276014	Illumina Paired End PCR Primer 2 (96% over 29bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	44316	0.1511920962520081	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	40148	0.13697220598261625	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	3.411681926437587E-6	0.0
3	0.0	0.0	0.0	1.0235045779312762E-5	0.0
4	0.0	0.0	0.0	4.094018311725105E-5	0.0
5	0.0	1.3646727705750349E-5	0.0	5.7998592749438985E-5	0.0
6	0.0	1.3646727705750349E-5	0.0	5.7998592749438985E-5	0.0
7	0.0	1.3646727705750349E-5	0.0	7.84686843080645E-5	3.411681926437587E-6
8	0.0	1.3646727705750349E-5	0.0	1.1599718549887797E-4	6.823363852875174E-6
9	0.0	1.7058409632187937E-5	0.0	2.115242794391304E-4	6.823363852875174E-6
10	0.0	1.7058409632187937E-5	0.0	4.6740042392194944E-4	6.823363852875174E-6
11	0.0	2.388177348506311E-5	0.0	5.970443371265778E-4	6.823363852875174E-6
12	0.0	2.388177348506311E-5	0.0	7.232765684047685E-4	6.823363852875174E-6
13	0.0	2.388177348506311E-5	0.0	8.59743845462272E-4	6.823363852875174E-6
14	1.0235045779312762E-5	2.388177348506311E-5	0.0	0.0010542097152692145	1.0235045779312762E-5
15	1.0235045779312762E-5	2.7293455411500697E-5	0.0	0.001422671363324474	1.0235045779312762E-5
16	1.0235045779312762E-5	2.7293455411500697E-5	0.0	0.001695605917439481	1.0235045779312762E-5
17	1.0235045779312762E-5	3.070513733793829E-5	0.0	0.0020572442016418653	1.3646727705750349E-5
18	1.0235045779312762E-5	3.752850119081346E-5	0.0	0.002487116124373001	1.3646727705750349E-5
19	1.0235045779312762E-5	3.752850119081346E-5	0.0	0.002787344133899509	1.3646727705750349E-5
20	1.7058409632187937E-5	4.094018311725105E-5	0.0	0.0031762758735133937	1.3646727705750349E-5
21	1.7058409632187937E-5	4.094018311725105E-5	0.0	0.0035890893866123417	1.3646727705750349E-5
22	1.7058409632187937E-5	4.094018311725105E-5	0.0	0.0040360197189756655	1.3646727705750349E-5
23	1.7058409632187937E-5	5.117522889656381E-5	0.0	0.004486361733265427	1.7058409632187937E-5
24	1.7058409632187937E-5	5.4586910823001394E-5	0.0	0.0048991752463643755	1.7058409632187937E-5
25	1.7058409632187937E-5	6.141027467587658E-5	0.0	0.0053597523064334495	1.7058409632187937E-5
26	2.0470091558625524E-5	6.141027467587658E-5	0.0	0.005871504595399087	2.0470091558625524E-5
27	2.0470091558625524E-5	6.482195660231416E-5	0.0	0.006564076026465917	2.0470091558625524E-5
28	2.388177348506311E-5	6.482195660231416E-5	0.0	0.007618285741735132	2.388177348506311E-5
29	2.7293455411500697E-5	7.164532045518933E-5	0.0	0.009129660835146984	2.388177348506311E-5
30	2.7293455411500697E-5	7.164532045518933E-5	0.0	0.01094467562001178	2.388177348506311E-5
31	3.752850119081346E-5	7.505700238162692E-5	0.0	0.013015566549359396	2.388177348506311E-5
32	4.4351865043688634E-5	7.505700238162692E-5	0.0	0.01540033221593927	2.388177348506311E-5
33	5.117522889656381E-5	7.505700238162692E-5	0.0	0.01939882343372412	2.388177348506311E-5
34	5.117522889656381E-5	7.505700238162692E-5	0.0	0.02507245047738983	3.070513733793829E-5
35	5.117522889656381E-5	7.505700238162692E-5	0.0	0.0332843688743251	3.070513733793829E-5
36	5.7998592749438985E-5	7.505700238162692E-5	0.0	0.046016765823790176	3.411681926437587E-5
37	6.141027467587658E-5	8.529204816093968E-5	0.0	0.06734318954595153	3.411681926437587E-5
38	6.482195660231416E-5	9.893877586669003E-5	0.0	0.09685423820963666	3.752850119081346E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCCGTCT	42145	0.0	35.155205	38
TATGCCG	42920	0.0	34.898087	35
ATGCCGT	43590	0.0	34.035404	36
CCGTCTT	44015	0.0	33.697426	39
TGCCGTC	44195	0.0	33.552387	37
CGTCTTC	45190	0.0	32.71877	40
TATCGTA	36520	0.0	32.698723	30
ACCGATA	36640	0.0	32.6143	25
ATCGTAT	37130	0.0	32.598972	31
TCGTATG	45880	0.0	32.581936	32
CCGATAT	36660	0.0	32.546566	26
ATATCGT	36665	0.0	32.535183	29
CGATATC	36830	0.0	32.423965	27
GATATCG	36910	0.0	32.407772	28
CGTATGC	46260	0.0	32.36295	33
GCGGGCT	48165	0.0	32.2378	8
AGACCGA	46875	0.0	32.075966	23
TGAGCGG	48735	0.0	32.045357	5
GACCGAT	46700	0.0	31.57782	24
CGGGCTG	50105	0.0	31.042149	9
>>END_MODULE