FastQCFastQC Report
Thu 26 May 2016
SRR522140_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR522140_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29954313
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCTGAGCGGGCTGGCAAGGCAGACCGATATCGTATGCCGTCTTCTGCT1558110.5201621549457669Illumina PCR Primer Index 1 (95% over 24bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT736400.24584105801391606No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT637850.2129409544461928No Hit
TCTCTGAGCGGGCTGGCAAGGCAGACCGATCTCGTATGCCGTCTTCTGCT516390.17239253659397896Illumina Paired End PCR Primer 2 (96% over 29bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT401780.13413093466707113No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG296200.033.6812235
GGTATCA341900.032.717321
GCCGTCT306500.032.62909338
CCGTCTT307100.032.52972839
CGTCTTC312050.032.02118340
ATGCCGT312200.031.99752836
TCGTATG312500.031.83577332
GACCGAT313200.031.78885824
TATCGTA227250.031.47913730
ATATCGT228000.031.38543329
CGTATGC319000.031.31825833
ACCGATA230250.031.27650825
ATCGTAT231400.031.17057231
TGCCGTC324050.030.88193737
TGAGCGG338750.030.846875
GCGGGCT337950.030.7362758
CGATATC233900.030.73367727
GATATCG235100.030.6522328
CCGATAT235100.030.57532326
AGACCGA331100.030.57342723
Produced by FastQC (version 0.11.5)