FastQCFastQC Report
Thu 26 May 2016
SRR522139_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR522139_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35161354
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCTGAGCGGGCTGGCAAGGCAGACCGATATCGTATGCCGTCTTCTGCT3174080.9027183651687589Illumina PCR Primer Index 1 (95% over 24bp)
TCTCTGAGCGGGCTGGCAAGGCAGACCGATCTCGTATGCCGTCTTCTGCT799940.2275054595451586Illumina Paired End PCR Primer 2 (96% over 29bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT587040.1669560279163311No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT526210.14965578401787372No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTCT544150.034.5395138
TATGCCG555850.034.17069235
CCGTCTT562200.033.47063439
ATGCCGT561500.033.45439536
TGCCGTC569650.032.9550237
CGTCTTC571300.032.8350440
TATCGTA458950.032.1462630
ATATCGT460100.032.0517729
ATCGTAT464750.031.98877731
TCGTATG593550.031.95957232
ACCGATA462500.031.937725
CCGATAT462800.031.9168626
CGTATGC597700.031.80731233
CGATATC466600.031.73369427
GCGGGCT627200.031.7000568
GATATCG467100.031.6991728
TGAGCGG630850.031.6314875
AGACCGA607800.031.57939723
GACCGAT602450.031.35433424
CGGGCTG648650.030.7093149
Produced by FastQC (version 0.11.5)