FastQCFastQC Report
Thu 26 May 2016
SRR522138_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR522138_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52785720
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA31150.014.7660581
AAGCAGT300050.012.6890411
TCAACGC294000.012.21419612
ATCAACG296150.012.04399511
CAACGCA301000.011.84993113
GGTATCA322050.011.2321698
GTATCAA322450.011.2318629
GTGGTAT330000.011.0126176
AGCAGTG349550.010.9153922
AACGCAG337400.010.66259214
TGGTATC349450.010.4561867
ACGCAGA346950.010.35438415
CGCAGAG345700.010.32834416
AGTGGTA362300.010.2004275
TATCAAC365050.010.02934610
AGAGTAC365650.09.74195419
GAGTACT252800.09.63053620
TCGTATG31450.09.4917713
CAGAGTA406650.08.93174318
CTCGTAT35100.08.75487312
Produced by FastQC (version 0.11.5)