##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR522138_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	52785720
Sequences flagged as poor quality	0
Sequence length	50
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	38.87359984859542	40.0	39.0	40.0	38.0	40.0
2	38.780202732860324	40.0	39.0	40.0	37.0	40.0
3	38.781025550091954	40.0	39.0	40.0	37.0	40.0
4	38.77422607856822	40.0	39.0	40.0	37.0	40.0
5	38.7759217644469	40.0	39.0	40.0	37.0	40.0
6	38.81897617385914	40.0	39.0	40.0	37.0	40.0
7	38.7844849326674	40.0	39.0	40.0	37.0	40.0
8	38.76337145728049	40.0	39.0	40.0	37.0	40.0
9	38.74150713488421	40.0	39.0	40.0	36.0	40.0
10	38.72134823584864	40.0	39.0	40.0	36.0	40.0
11	38.75064587543752	40.0	39.0	40.0	36.0	40.0
12	38.7105312383728	40.0	39.0	40.0	36.0	40.0
13	38.677505734505466	40.0	39.0	40.0	36.0	40.0
14	38.643922522985385	40.0	39.0	40.0	36.0	40.0
15	38.625606565563565	40.0	39.0	40.0	36.0	40.0
16	38.611957305877425	40.0	39.0	40.0	36.0	40.0
17	38.56132095953224	40.0	39.0	40.0	36.0	40.0
18	38.51662322688788	40.0	39.0	40.0	35.0	40.0
19	38.477032500456566	40.0	38.0	40.0	35.0	40.0
20	38.43034936342632	40.0	38.0	40.0	35.0	40.0
21	38.35438607259691	40.0	38.0	40.0	35.0	40.0
22	38.27336326946	40.0	38.0	40.0	35.0	40.0
23	38.20844148758414	40.0	38.0	40.0	35.0	40.0
24	38.15343039746356	40.0	38.0	40.0	35.0	40.0
25	38.16880942421549	40.0	38.0	40.0	35.0	40.0
26	38.06097921937979	40.0	38.0	40.0	35.0	40.0
27	37.97026133204208	40.0	38.0	40.0	35.0	40.0
28	37.9726599542452	40.0	38.0	40.0	35.0	40.0
29	37.90587200477705	40.0	38.0	40.0	35.0	40.0
30	37.82802566679018	40.0	38.0	40.0	34.0	40.0
31	37.78349320611711	40.0	38.0	40.0	35.0	40.0
32	37.70658285990984	40.0	38.0	40.0	34.0	40.0
33	37.719693261738215	40.0	38.0	40.0	34.0	40.0
34	37.649684782172145	40.0	38.0	40.0	33.0	40.0
35	37.56294605813845	40.0	38.0	40.0	33.0	40.0
36	37.629469504252285	40.0	38.0	40.0	34.0	40.0
37	37.54695205445715	40.0	38.0	40.0	33.0	40.0
38	37.45896185559276	40.0	38.0	40.0	33.0	40.0
39	37.285144732325335	39.0	38.0	40.0	33.0	40.0
40	37.28824723807878	39.0	38.0	40.0	33.0	40.0
41	37.02577797555854	39.0	38.0	40.0	33.0	40.0
42	36.810535103054384	39.0	37.0	40.0	32.0	40.0
43	36.60978304359588	39.0	37.0	40.0	32.0	40.0
44	36.70189939627611	39.0	37.0	40.0	32.0	40.0
45	36.60165876301394	39.0	37.0	40.0	32.0	40.0
46	36.37098084481939	39.0	36.0	40.0	31.0	40.0
47	36.23069583970816	39.0	36.0	40.0	31.0	40.0
48	36.110334025944894	39.0	36.0	40.0	31.0	40.0
49	35.97343423562282	39.0	36.0	40.0	30.0	40.0
50	35.85336674388452	39.0	36.0	40.0	30.0	40.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1	1	-0.14514896872944405
1	2	-0.15925264525478866
1	3	-0.14707743660292039
1	4	-0.1549309305595017
1	5	-0.15960426943629358
1	6	-0.17251946840607246
1	7	-0.15167791717832557
1	8	-0.16266844851242723
1	9	-0.1450595245604518
1	10	-0.17676015939983358
1	11	-0.15455945930392545
1	12	-0.17801597572182004
1	13	-0.14848822205939172
1	14	-0.19798674309091524
1	15	-0.16837473469993824
1	16	-0.1784758037772889
1	17	-0.18539227357111088
1	18	-0.17715477689733206
1	19	-0.1834693462319379
1	20	-0.20645758310215
1	21	-0.20095971101803656
1	22	-0.20369188833900864
1	23	-0.21242729228369228
1	24	-0.21161371198421364
1	25	-0.20774155055909915
1	26	-0.1865919050551028
1	27	-0.20414990906965613
1	28	-0.2186232230176799
1	29	-0.24076173280106872
1	30	-0.23852353442536867
1	31	-0.19631411214043482
1	32	-0.21960368756003135
1	33	-0.18908075889722653
1	34	-0.1758956581747242
1	35	-0.14652256153431864
1	36	-0.07031396269623968
1	37	-0.09765817284905864
1	38	-0.08211605771874275
1	39	0.050827669754582416
1	40	-0.11937235971749516
1	41	0.04605103940316724
1	42	0.16077428260640403
1	43	0.23709098905016646
1	44	0.022433348571617273
1	45	0.009287516097209902
1	46	-0.061498622996218444
1	47	-0.057813537387033875
1	48	-0.10349767003312849
1	49	-0.11487285845990414
1	50	-0.10879847634795681
2	1	-0.1281200137429508
2	2	-0.13723609207342236
2	3	-0.14662666512677447
2	4	-0.1458200383086279
2	5	-0.1436156726400526
2	6	-0.1609419732664179
2	7	-0.15955512297963992
2	8	-0.1545156346719807
2	9	-0.1560270509050099
2	10	-0.1717133539036979
2	11	-0.15426049119199803
2	12	-0.16330612489601748
2	13	-0.16952136070689505
2	14	-0.1690412472061098
2	15	-0.18863507055804263
2	16	-0.17389323102244703
2	17	-0.1750677986346716
2	18	-0.18701359039860677
2	19	-0.19655010565881526
2	20	-0.218273670121107
2	21	-0.20939604637739961
2	22	-0.20865082916102295
2	23	-0.2151914531449748
2	24	-0.20901142028499464
2	25	-0.1873107733756001
2	26	-0.27760106019974984
2	27	-0.2722727704355066
2	28	-0.30308280934956855
2	29	-0.3083355675156625
2	30	-0.3102040291171164
2	31	-0.2780871602198829
2	32	-0.2997429499606312
2	33	-0.29072246733309015
2	34	-0.253984169657528
2	35	-0.26465005223946036
2	36	-0.2159644711442752
2	37	-0.21590515431574175
2	38	-0.23353186553009664
2	39	-0.11614970381621248
2	40	-0.2027431514254303
2	41	-0.06683603922954973
2	42	0.040151828511078236
2	43	0.13773596084552509
2	44	-0.0712678091157386
2	45	-0.10640373610288378
2	46	-0.12107485197910961
2	47	-0.17743572021606013
2	48	-0.1881517350012203
2	49	-0.1695280533949841
2	50	-0.20308994437360184
3	1	-0.10016316130609937
3	2	-0.10265686790272355
3	3	-0.10530646448024328
3	4	-0.11400099308232114
3	5	-0.10390787901186371
3	6	-0.11812982463145971
3	7	-0.12477340665958536
3	8	-0.14454205733996872
3	9	-0.11569994975800313
3	10	-0.1384316655764266
3	11	-0.14841279539882635
3	12	-0.1271793606413425
3	13	-0.1543511508433113
3	14	-0.1736284793171663
3	15	-0.17282917561529842
3	16	-0.13667161003216677
3	17	-0.1555187575462682
3	18	-0.14425332783279288
3	19	-0.15712531443787014
3	20	-0.16762394021318983
3	21	-0.1599349932050913
3	22	-0.18035822229021647
3	23	-0.1825011356449906
3	24	-0.174762664722941
3	25	-0.1651386661216776
3	26	-0.19775839355493474
3	27	-0.1901470088220094
3	28	-0.21569953643384565
3	29	-0.23350181436995854
3	30	-0.2499861480776957
3	31	-0.20788146921141504
3	32	-0.25986659204316
3	33	-0.2306131003781431
3	34	-0.20781838326490032
3	35	-0.18009653060678232
3	36	-0.09878857951137832
3	37	-0.15408779443490062
3	38	-0.1319843033510253
3	39	0.019686728410107435
3	40	-0.12288341207474218
3	41	0.025826433868409993
3	42	0.12961009974163318
3	43	0.2718319152651887
3	44	0.06677202533968796
3	45	0.024588857589051827
3	46	-0.0031842802224346656
3	47	-0.05053784208910628
3	48	-0.02487679434438661
3	49	-0.06554825949050525
3	50	-0.07740402270871982
4	1	-0.08908088447230256
4	2	-0.07186927192747561
4	3	-0.08815962905501351
4	4	-0.08076597911006189
4	5	-0.09754221787999029
4	6	-0.09577247568821434
4	7	-0.10642954683431327
4	8	-0.08628476884825176
4	9	-0.10511450504618836
4	10	-0.09089384820072866
4	11	-0.09575835884555772
4	12	-0.0770231424713188
4	13	-0.09054926207440417
4	14	-0.13702136860094782
4	15	-0.1207198234479776
4	16	-0.11714644210091052
4	17	-0.11463232545948188
4	18	-0.10831691806880173
4	19	-0.12279785596881254
4	20	-0.10275426063387272
4	21	-0.10394647830591452
4	22	-0.1227394332434315
4	23	-0.10930196847755269
4	24	-0.11173899801153908
4	25	-0.11376786254115245
4	26	-0.14160455069001188
4	27	-0.14628863518191082
4	28	-0.1950379025212925
4	29	-0.12194826594246422
4	30	-0.11715306160304095
4	31	-0.13266210657161537
4	32	-0.11720347049613622
4	33	-0.1258644107474396
4	34	-0.1472635918778451
4	35	-0.09775889357883472
4	36	-0.038620440680574575
4	37	-0.07613776880195644
4	38	-0.030418228782004064
4	39	0.044743669805505704
4	40	-0.04256609464960093
4	41	0.07579080842955221
4	42	0.18591989527533315
4	43	0.31863541138549323
4	44	0.1050500525813618
4	45	0.09196295862599158
4	46	0.05291656583691662
4	47	0.053276941764607955
4	48	0.03863015702971495
4	49	0.009185039343762469
4	50	0.006888482694748177
5	1	-0.08798289145415339
5	2	-0.10364063865105777
5	3	-0.09180958246390958
5	4	-0.08092362044293822
5	5	-0.07764869048954637
5	6	-0.08129889091324571
5	7	-0.09181508565345808
5	8	-0.09399026570702063
5	9	-0.09086139873750199
5	10	-0.10579648137733244
5	11	-0.08924017856380573
5	12	-0.09143647415439915
5	13	-0.09351040212983719
5	14	-0.10162226269324037
5	15	-0.09421409253494062
5	16	-0.09179778716590903
5	17	-0.09513288964768662
5	18	-0.09094491022432294
5	19	-0.10477516418391986
5	20	-0.11833841386349775
5	21	-0.08204011491564245
5	22	-0.0849375674818802
5	23	-0.09454023608458328
5	24	-0.10865735873739624
5	25	-0.09084460394222305
5	26	-0.07824637074119067
5	27	-0.08806503451787506
5	28	-0.08875028525865503
5	29	-0.11209947903676465
5	30	-0.06489043811624384
5	31	-0.12451366947619391
5	32	-0.1122681801889982
5	33	-0.06810869485283888
5	34	-0.09419820646857602
5	35	-0.10564651312601825
5	36	-0.03807724694665637
5	37	-0.09420809819307152
5	38	-0.045980147762271883
5	39	0.07859364108676914
5	40	-0.09386679910737428
5	41	0.11477224212612924
5	42	0.21946925581386267
5	43	0.3101453427589931
5	44	0.11156694867806038
5	45	0.06767651278389764
5	46	0.05729099028258844
5	47	0.023127159470107017
5	48	0.06065533931521827
5	49	0.054232382501581355
5	50	-0.004908202237700721
6	1	-0.0743714418923247
6	2	-0.057932634420765794
6	3	-0.05322851922104377
6	4	-0.06285962755038099
6	5	-0.050524137686146275
6	6	-0.0450326961091676
6	7	-0.050269917387204544
6	8	-0.04887256059333822
6	9	-0.04563006854114349
6	10	-0.06859887823537747
6	11	-0.04278927397827914
6	12	-0.0635767742792055
6	13	-0.06134405645406815
6	14	-0.05651376317159418
6	15	-0.050108490537290606
6	16	-0.07316907050516619
6	17	-0.0584744569568727
6	18	-0.06865189821929363
6	19	-0.055784042743034945
6	20	-0.06091227787160136
6	21	-0.07542204329980251
6	22	-0.08446258457671263
6	23	-0.09198129828391899
6	24	-0.08219039549621243
6	25	-0.06272229133277563
6	26	-0.0790525778278024
6	27	-0.06654655552427613
6	28	-0.06988677488788397
6	29	-0.07983624639938824
6	30	-0.04449705810512228
6	31	-0.10467614163692929
6	32	-0.09892212416227864
6	33	-0.04114955673898635
6	34	-0.08802014029751604
6	35	-0.09118104529739668
6	36	-0.014116376596120972
6	37	-0.024634256412866762
6	38	-0.03828158101576662
6	39	0.11992150283098368
6	40	-0.011720210248363117
6	41	0.18103931116183247
6	42	0.2652828718730973
6	43	0.3889609265580276
6	44	0.18978608704594535
6	45	0.156512244729889
6	46	0.13378324955835552
6	47	0.08282708110422021
6	48	0.13567787021325728
6	49	0.11392687119725764
6	50	0.11306609570523563
7	1	-0.06771529089920136
7	2	-0.08637100117880436
7	3	-0.0596800294640758
7	4	-0.07475988689895274
7	5	-0.07218655088608728
7	6	-0.08733846630185838
7	7	-0.0879663590208466
7	8	-0.08310544769493333
7	9	-0.1084523321435995
7	10	-0.08960186246205382
7	11	-0.07379930534864343
7	12	-0.07535678348941133
7	13	-0.08158014714472728
7	14	-0.08879477554915383
7	15	-0.08064398545862872
7	16	-0.0913443235889062
7	17	-0.08652112903221365
7	18	-0.08737966478460635
7	19	-0.09509310070391308
7	20	-0.0813274126749235
7	21	-0.09279849636421034
7	22	-0.09231206415815763
7	23	-0.08571765037778789
7	24	-0.09869539454695797
7	25	-0.0646800721476879
7	26	-0.06732275943537758
7	27	-0.07259918978962787
7	28	-0.12353621852153651
7	29	-0.12418665749709135
7	30	-0.07940019240002982
7	31	-0.1063593981017874
7	32	-0.161638355794544
7	33	-0.13801848516529702
7	34	-0.08883171829132408
7	35	-0.10364239087945748
7	36	-0.05078273097043251
7	37	-0.09500126337194104
7	38	-0.050924103949910204
7	39	0.061795171674326355
7	40	-0.04692654712500399
7	41	0.07251578886633325
7	42	0.19424777197507836
7	43	0.31315761357031846
7	44	0.12963618625675366
7	45	0.11637769822989696
7	46	-0.009306200497675832
7	47	0.014895022747175801
7	48	0.00361555681690362
7	49	-0.009878859069523571
7	50	-0.019204252576699332
8	1	-0.06446013555001429
8	2	-0.06422048785906043
8	3	-0.06143725775163489
8	4	-0.08266408461183516
8	5	-0.09066991481834208
8	6	-0.07895506610519476
8	7	-0.07565710066773335
8	8	-0.06780969766315792
8	9	-0.05988780598276833
8	10	-0.09064522927925367
8	11	-0.057893012386628584
8	12	-0.07091000990743623
8	13	-0.060690014969566164
8	14	-0.06309408902688318
8	15	-0.06291413384527544
8	16	-0.05631281649689868
8	17	-0.05717252293118236
8	18	-0.05165904224308804
8	19	-0.06452556604385506
8	20	-0.05992641924505193
8	21	-0.08409565762579518
8	22	-0.07938607118052943
8	23	-0.11341576159382072
8	24	-0.10991943822517669
8	25	-0.29898471439913266
8	26	-0.07545278793932653
8	27	-0.10820258526567983
8	28	-0.07023200949466712
8	29	-0.10248428711554425
8	30	-0.102242215814492
8	31	-0.11337535796765508
8	32	-0.09983218659015591
8	33	-0.0964903624713429
8	34	-0.0491732982091051
8	35	-0.03537681173938978
8	36	-0.012991583659271555
8	37	-0.05374338769315301
8	38	6.475045895939502E-4
8	39	0.10396696660809823
8	40	-0.007701445922364769
8	41	0.18261742077665843
8	42	0.2767225797276396
8	43	0.3808671206263057
8	44	0.16902121790305102
8	45	0.15186160871611776
8	46	0.09741815773097784
8	47	0.09881014439201863
8	48	0.11844083644971448
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113	21	-0.07548760291911094
113	22	-0.061283116693950035
113	23	-0.08229065837291216
113	24	-0.053647839197957126
113	25	-0.04822582963810618
113	26	-0.0566757513905074
113	27	-0.021285509079383758
113	28	-0.053525727134008605
113	29	-0.040257919088844574
113	30	0.017785713928027747
113	31	0.004195403967919731
113	32	-0.012167777727640328
113	33	0.03324762266670689
113	34	0.04153588994910962
113	35	0.08138500684356131
113	36	0.11016801109546037
113	37	0.09846850649163486
113	38	0.12069081144203864
113	39	0.2411525630979625
113	40	0.17790900257067221
113	41	0.3190880533535818
113	42	0.42218873796124257
113	43	0.5215001249505349
113	44	0.32557360807372504
113	45	0.33045660797144905
113	46	0.2957353086669201
113	47	0.27735033724471947
113	48	0.25551013668574285
113	49	0.3080436986599011
113	50	0.2644376303558502
114	1	-0.055876507986681645
114	2	-0.036592265038798644
114	3	-0.054530378013993186
114	4	-0.05208136083479076
114	5	-0.057018545296003253
114	6	-0.048007875961367574
114	7	-0.05648030900416501
114	8	-0.07216141603475279
114	9	-0.05826269843698384
114	10	-0.05102370388470945
114	11	-0.06401954082976857
114	12	-0.05654982671092057
114	13	-0.07314324271845862
114	14	-0.048517840710246674
114	15	-0.06218797523814601
114	16	-0.060164549830126646
114	17	-0.04541954874519405
114	18	-0.03884427270600099
114	19	-0.06332354753210012
114	20	-0.05134959282263196
114	21	-0.03220659498569489
114	22	-0.018620387298661
114	23	-0.04301264019920126
114	24	-0.07994756336240272
114	25	-0.021267409390546277
114	26	-0.01748981023582985
114	27	0.002829343046670374
114	28	-0.00805554374915829
114	29	0.002201084847165191
114	30	-0.03206529526358537
114	31	-0.04517820026562447
114	32	0.012260658116012735
114	33	0.028178360157639304
114	34	0.037272418599073376
114	35	0.05335253902237724
114	36	0.08480968490923857
114	37	0.11961904472776297
114	38	0.12395961516653387
114	39	0.23968100427116212
114	40	0.14603694635356135
114	41	0.31008746735989945
114	42	0.42608774601364274
114	43	0.512111732668771
114	44	0.33321431167263427
114	45	0.3186799437579353
114	46	0.28742238895565464
114	47	0.36075196197766246
114	48	0.3381989188996215
114	49	0.3792045662500527
114	50	0.3437838918493412
115	1	-0.08412483995473252
115	2	-0.08357157364226708
115	3	-0.0754184158359692
115	4	-0.09329923362449932
115	5	-0.08125782697119632
115	6	-0.0993356715932805
115	7	-0.09351594966744159
115	8	-0.08932233830851999
115	9	-0.08916097605300877
115	10	-0.09488656145343555
115	11	-0.08701595735983148
115	12	-0.09110701348077299
115	13	-0.1096273502731151
115	14	-0.10107101797011353
115	15	-0.09694300122299637
115	16	-0.0930377745008002
115	17	-0.0781816793437713
115	18	-0.09288186824557698
115	19	-0.08496309890016107
115	20	-0.10131358012665004
115	21	-0.08761361934852374
115	22	-0.1148460891530263
115	23	-0.11038057254548761
115	24	-0.0676809142734669
115	25	-0.05403434705919352
115	26	-0.05111344195494638
115	27	-0.05623898973282593
115	28	-0.060537589017783944
115	29	-0.05310419812556688
115	30	-0.011993002534950392
115	31	-0.05266288439869271
115	32	-0.04220445051698363
115	33	0.0030013657347751632
115	34	0.03624925261925682
115	35	0.030375232005638964
115	36	0.06154552670970048
115	37	0.0656218342160102
115	38	0.041481418527403946
115	39	0.1764657772258289
115	40	0.11813719788345622
115	41	0.27749272123080715
115	42	0.4014208145348874
115	43	0.505978961373124
115	44	0.2747102525317473
115	45	0.31241224041513505
115	46	0.2814032152364163
115	47	0.2884119735073156
115	48	0.3040080836728549
115	49	0.3414021381010457
115	50	0.2865426498431134
116	1	-0.09428354672878925
116	2	-0.07775972141421761
116	3	-0.09876885357803644
116	4	-0.06813679980995602
116	5	-0.08187088071095872
116	6	-0.07440756198965914
116	7	-0.09895149664430392
116	8	-0.10317821443423014
116	9	-0.10069104586117561
116	10	-0.08716244037966447
116	11	-0.08635696623166922
116	12	-0.08539627153454887
116	13	-0.09638583463815564
116	14	-0.08501531395878459
116	15	-0.0968882602362342
116	16	-0.09963068961261001
116	17	-0.08817145121712855
116	18	-0.09983893921856435
116	19	-0.10871623232356598
116	20	-0.1275454250066872
116	21	-0.14570839439974037
116	22	-0.11438477211332554
116	23	-0.12366568359954755
116	24	-0.14230312365370423
116	25	-0.09331303986153472
116	26	-0.08351529659776702
116	27	-0.10753191970595566
116	28	-0.10447733445828078
116	29	-0.10371608766899953
116	30	-0.09413365547497676
116	31	-0.09778375114320426
116	32	-0.03969668167023599
116	33	-0.03841673299379522
116	34	-0.018731926857235237
116	35	0.018805431027722364
116	36	0.06941641204146975
116	37	0.06748149615206245
116	38	0.0901620073303917
116	39	0.1528640045994507
116	40	0.09211379251380691
116	41	0.25584429016101495
116	42	0.372758644471908
116	43	0.48913430390827983
116	44	0.24638490092960552
116	45	0.23518430762510434
116	46	0.1987883754565658
116	47	0.20840760132216474
116	48	0.23917712947768166
116	49	0.21497874389138616
116	50	0.21802629299494214
117	1	-0.1427366370269283
117	2	-0.11865616033397686
117	3	-0.10336713016700827
117	4	-0.10827080791977295
117	5	-0.11897078517088033
117	6	-0.11832221968195
117	7	-0.1207654411702137
117	8	-0.11070625428244796
117	9	-0.11675484261963476
117	10	-0.13450864065566037
117	11	-0.10898377730391928
117	12	-0.1112967639926481
117	13	-0.110031872592252
117	14	-0.11230154681519622
117	15	-0.114907206531349
117	16	-0.11737589031893947
117	17	-0.13030968621028194
117	18	-0.1157956317194575
117	19	-0.1207107601529529
117	20	-0.11628982839164337
117	21	-0.11225036388918141
117	22	-0.1411513538444069
117	23	-0.16006600305951224
117	24	-0.15528278925750527
117	25	-0.0804446108621164
117	26	-0.09047192777141788
117	27	-0.10596867254037079
117	28	-0.09220727760178704
117	29	-0.0913807414986394
117	30	-0.10045163915442856
117	31	-0.06690593334587902
117	32	-0.06292483518048897
117	33	-0.05584784254926234
117	34	-0.0047516522988004795
117	35	0.020370119826964128
117	36	0.06709612053733593
117	37	0.07902115261618547
117	38	0.08774302425154445
117	39	0.19235823763808924
117	40	0.12012694465244778
117	41	0.2664986309453923
117	42	0.3788099637628335
117	43	0.4772479456907064
117	44	0.2614178350273946
117	45	0.27334372747741753
117	46	0.25168922679274175
117	47	0.2513454183063004
117	48	0.26384484353527426
117	49	0.30335621976843896
117	50	0.2709947051954842
118	1	-0.16147022440046044
118	2	-0.13876569240463965
118	3	-0.14473045924057715
118	4	-0.14391604643630984
118	5	-0.15648437171662266
118	6	-0.13435875318274526
118	7	-0.13427668287797445
118	8	-0.13676829573338267
118	9	-0.15608069026080784
118	10	-0.15524946651120075
118	11	-0.14277534063118935
118	12	-0.14574356936378763
118	13	-0.15456933377439697
118	14	-0.1383585993416787
118	15	-0.14935498276090442
118	16	-0.14189039725560093
118	17	-0.1519976680713384
118	18	-0.1667625864422817
118	19	-0.17929964916617536
118	20	-0.18297238728303
118	21	-0.18530949868410573
118	22	-0.17740143715290202
118	23	-0.17895173757700888
118	24	-0.1856043198069841
118	25	-0.17384631259687922
118	26	-0.10488101072737521
118	27	-0.10228730412251252
118	28	-0.1553638288358954
118	29	-0.1451080419514028
118	30	-0.1297648847397994
118	31	-0.1340870397400451
118	32	-0.12240328304124404
118	33	-0.10323583392937508
118	34	-0.07720589171009351
118	35	-0.06621788139351992
118	36	0.007205221332796441
118	37	-0.005698063228884109
118	38	0.015351170646560774
118	39	0.11865297148182918
118	40	0.03368734386522476
118	41	0.19804405266354053
118	42	0.3407898642433125
118	43	0.41548564665008314
118	44	0.19848777226702907
118	45	0.19956225269866934
118	46	0.1898764188640314
118	47	0.13907195873581202
118	48	0.197370708126833
118	49	0.21208964850571732
118	50	0.22377002415864666
119	1	-0.14613787574572967
119	2	-0.1700520470461555
119	3	-0.1598447871337143
119	4	-0.1558275302016341
119	5	-0.17079162973245587
119	6	-0.14850640938647786
119	7	-0.15329083073869043
119	8	-0.16421954179859455
119	9	-0.17372747281898882
119	10	-0.17175532313216024
119	11	-0.1533620384487051
119	12	-0.15298208710223804
119	13	-0.158650492286597
119	14	-0.17969263295918125
119	15	-0.16936516080415487
119	16	-0.17963023452757199
119	17	-0.17593878536163743
119	18	-0.1959881339842866
119	19	-0.18744420743846746
119	20	-0.19028036255492253
119	21	-0.21547650574991906
119	22	-0.21611709796327716
119	23	-0.21724311622324421
119	24	-0.20348655391371295
119	25	-0.18460393263598718
119	26	-0.14528009549059817
119	27	-0.1812021829906243
119	28	-0.20764055584947272
119	29	-0.1965651117101146
119	30	-0.17155250289887647
119	31	-0.1919896249452293
119	32	-0.16335463868297495
119	33	-0.11269386522207014
119	34	-0.12408161369327075
119	35	-0.09024952829036437
119	36	-0.04603644526522288
119	37	-0.04646352497724848
119	38	-0.03903703221279642
119	39	0.06806310769069768
119	40	-0.03637017501321793
119	41	0.13063860095933677
119	42	0.22330656845026908
119	43	0.37951059612430527
119	44	0.15260101986288532
119	45	0.14923096636422883
119	46	0.11227132309654309
119	47	0.06544721895777172
119	48	0.1025432291847963
119	49	0.09490472369078162
119	50	0.06947495766767986
120	1	-0.16090958754331552
120	2	-0.17548010695965388
120	3	-0.15797929187072413
120	4	-0.1653375300571156
120	5	-0.17431141641907288
120	6	-0.15876564474300636
120	7	-0.16797717313488647
120	8	-0.1705847737497237
120	9	-0.15339023115328843
120	10	-0.16903160126381778
120	11	-0.17513415307615787
120	12	-0.17547298493115449
120	13	-0.15335139672399833
120	14	-0.16716804570236832
120	15	-0.15587433696652653
120	16	-0.17437077582434313
120	17	-0.17982430181596243
120	18	-0.16927521488922537
120	19	-0.18062801434777498
120	20	-0.17435698828523272
120	21	-0.18339991565360947
120	22	-0.23122194590595058
120	23	-0.21094202323899225
120	24	-0.20939598623054678
120	25	-0.18077946627141017
120	26	-0.14944954242647412
120	27	-0.1830362000588508
120	28	-0.14781814927351178
120	29	-0.2075595893573805
120	30	-0.1902521659061165
120	31	-0.17640250181145944
120	32	-0.1471951854136293
120	33	-0.09135714051055288
120	34	-0.09670518289870955
120	35	-0.04248525494912059
120	36	0.011868936265308605
120	37	-0.005712196500809341
120	38	-0.00838014302110679
120	39	0.1215898603921417
120	40	0.034086541659462455
120	41	0.20677905673897357
120	42	0.33764322458721807
120	43	0.45306309886947105
120	44	0.19829036237203468
120	45	0.20561130807268313
120	46	0.1616794871495273
120	47	0.15945695953036676
120	48	0.18958860009689715
120	49	0.1852758938366037
120	50	0.14295865828604093
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	32815.0
3	1527.0
4	2520.0
5	2827.0
6	4528.0
7	8012.0
8	9578.0
9	10624.0
10	14959.0
11	21082.0
12	22845.0
13	30109.0
14	39205.0
15	45824.0
16	48060.0
17	53841.0
18	63162.0
19	74605.0
20	88536.0
21	81730.0
22	102053.0
23	127987.0
24	150433.0
25	174921.0
26	226545.0
27	278075.0
28	316496.0
29	360580.0
30	430327.0
31	518575.0
32	619036.0
33	781259.0
34	1080714.0
35	1587977.0
36	2491452.0
37	4459412.0
38	8358276.0
39	2.9580104E7
40	485109.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	20.533375196234722	28.096186118630843	27.36114616937075	24.009292515763683
2	23.817290837958033	27.617883566455724	26.850246948730057	21.714578646856193
3	23.405901397997056	26.631586054561556	26.81058731441125	23.151925233030134
4	24.386399515464646	26.099996229960297	26.329252533741382	23.184351720833675
5	24.889421609631256	27.011298340097483	26.483550542069878	21.61572950820138
6	24.7270682298167	26.330001750473425	25.1395453164227	23.80338470328718
7	24.231955060098112	26.290553784089038	26.74058252113771	22.73690863467514
8	23.78261961757839	26.31398226641599	26.6242631529891	23.279134963016514
9	23.469660479078488	26.051518298374603	26.974554761754288	23.504266460792618
10	23.502726173841545	26.692392951409456	26.34846269544734	23.45641817930166
11	23.70522936885203	26.54322229572695	26.37601230029637	23.37553603512465
12	23.53432140359173	26.35239227578974	26.653261526033933	23.460024794584598
13	23.4519657968102	26.41676006313829	26.700088584564156	23.431185555487353
14	23.486375573936115	26.477207091423967	26.79336401298848	23.24305332165144
15	23.398198305926076	26.32814211224891	26.72699669497322	23.546662886851795
16	23.45554172626534	26.225272450162603	26.826113105727156	23.4930727178449
17	23.705193374268646	26.154588778934908	26.841395741120895	23.29882210567555
18	23.443704092697796	26.040987979324708	26.811162185530478	23.70414574244701
19	23.570151169672403	26.19723667688913	26.808634986886602	23.42397716655186
20	23.73441339816905	25.977330611385046	26.847772465735055	23.44048352471085
21	23.52921585610654	26.15955413699008	26.7513012989119	23.559928707991478
22	23.764331088197956	26.01309434655205	26.643513417966513	23.579061147283483
23	23.576287937395847	26.109251121885336	26.842848074306815	23.471612866412002
24	23.5598860985887	26.093409122362175	26.644098063730947	23.70260671531818
25	23.668694164765945	26.04351255804197	26.626408472825663	23.661384804366428
26	23.803958752594685	26.073541998558053	26.730463088150703	23.392036160696563
27	23.77413394146217	25.93413701771039	26.65052268076384	23.641206360063595
28	23.850196888660523	26.09040323403396	26.514470389299046	23.54492948800647
29	23.81944896565325	26.146041378039804	26.586663778147308	23.447845878159637
30	23.76084432690072	25.9762640197683	26.605495457848395	23.657396195482587
31	23.814452342291833	26.053949198238612	26.557851866388916	23.57374659308064
32	23.81199538857615	26.062314499561566	26.560438502280505	23.565251609581782
33	23.775864749172502	26.06958752137465	26.440585106181302	23.713962623271545
34	23.799487626672974	26.155270350253186	26.46707535202566	23.57816667104818
35	23.818116745150036	26.130697459917602	26.448128408458228	23.603057386474134
36	23.744717543313563	26.0598290719771	26.483657418133117	23.711795966576226
37	23.811762776043935	26.11827450601083	26.428722992644577	23.641239725300657
38	23.842213935513602	26.098841637349533	26.471337972641074	23.587606454495788
39	23.822832733648433	25.97516036846971	26.445491759430734	23.756515138451125
40	23.839068433854756	26.059979099924597	26.451865937641756	23.649086528578895
41	23.834830165633157	26.137584745564347	26.407742163750903	23.61984292505159
42	23.803610958769646	26.032040976778596	26.35973079598716	23.804617268464597
43	23.879338103637483	26.07297853172712	26.315413241545254	23.732270123090146
44	23.83768484902072	26.07541646024707	26.397682327320986	23.689216363411223
45	23.80475096649597	25.99296257671142	26.35660047194418	23.845685984848426
46	23.882439054052114	26.126809126734123	26.24905478012711	23.74169703908665
47	23.889731726705886	26.140842136827942	26.263247654215267	23.706178482250905
48	23.827285695204477	25.98293080734342	26.269965855426907	23.919817642025198
49	23.869282744302474	26.07237181103425	26.189229727007852	23.869115717655422
50	23.788924544083933	26.156540395542343	26.26710634376636	23.78742871660736
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	8643.0
1	5588.0
2	2533.0
3	2224.5
4	1916.0
5	2110.5
6	2305.0
7	3185.5
8	4066.0
9	6326.5
10	8587.0
11	13972.0
12	19357.0
13	30483.5
14	41610.0
15	62203.5
16	82797.0
17	122901.0
18	163005.0
19	226184.0
20	289363.0
21	383031.0
22	476699.0
23	594240.5
24	711782.0
25	784006.5
26	856231.0
27	983110.5
28	1109990.0
29	1232579.0
30	1355168.0
31	1503093.5
32	1651019.0
33	1802419.5
34	1953820.0
35	2123018.5
36	2292217.0
37	2422752.0
38	2553287.0
39	2668552.0
40	2783817.0
41	2879790.0
42	2975763.0
43	3031078.0
44	3086393.0
45	3119189.0
46	3151985.0
47	3142100.5
48	3132216.0
49	3141437.5
50	3150659.0
51	3145116.5
52	3139574.0
53	3133248.0
54	3126922.0
55	3043899.0
56	2960876.0
57	2835077.0
58	2709278.0
59	2546940.5
60	2384603.0
61	2159502.5
62	1934402.0
63	1695372.5
64	1456343.0
65	1273472.5
66	1090602.0
67	937724.0
68	784846.0
69	651533.5
70	518221.0
71	427378.5
72	336536.0
73	271334.0
74	206132.0
75	158814.5
76	111497.0
77	88533.5
78	65570.0
79	51324.0
80	37078.0
81	29915.0
82	22752.0
83	18659.0
84	14566.0
85	12089.5
86	9613.0
87	7769.5
88	5926.0
89	4053.5
90	2181.0
91	1530.0
92	879.0
93	603.0
94	327.0
95	278.0
96	229.0
97	451.0
98	673.0
99	769.5
100	866.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.2487471990530772
2	0.03196319004458024
3	1.0987820190763713E-4
4	0.0021407304854418963
5	1.7050065813254038E-4
6	0.0
7	3.8267925491970175E-4
8	0.0
9	9.093368433735488E-5
10	3.4100131626508076E-5
11	0.0
12	0.0
13	0.0
14	0.018999456671236085
15	1.2503381596386294E-4
16	2.0838969327310492E-5
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	7.388361852410084E-5
23	8.866034222892101E-4
24	0.002794316341616634
25	0.007736940975703277
26	0.031874150811999916
27	0.03816751954884768
28	0.04194884525587602
29	0.04759810039533419
30	0.05098538013690066
31	0.06002191501792531
32	0.05641677332430059
33	0.06880648781526519
34	0.04952665228398893
35	0.026982676375353034
36	0.033901214192020114
37	0.032018129145534056
38	0.034518805464811314
39	0.3805612578553442
40	0.042197018436046715
41	0.04455561087354686
42	0.4118727564955067
43	0.5125439228639868
44	0.11249065088057907
45	0.1473731910827398
46	0.1786013338455931
47	0.16813069898449806
48	0.1917431456840979
49	0.18852826105242101
50	0.20052392957792373
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	5.278572E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.75943976646281
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.82111827555339	30.990924593726167
2	15.172706489344398	13.278982714293711
3	5.705375685021193	7.48992130901179
4	2.7589096873959345	4.829133691468525
5	1.4783099129411337	3.234500679575621
6	0.864043638799351	2.268603934058133
7	0.5500049196181248	1.6847535005901286
8	0.3838339895911858	1.3437088278269287
9	0.27828602218020076	1.0959876382898723
>10	1.6098915807884668	13.577083391754627
>50	0.2240605970252305	6.908482637320429
>100	0.14884144708706912	11.637228175133231
>500	0.003837026822149091	1.0694636109150444
>1k	7.590689060571457E-4	0.44677394578722907
>5k	1.2995373266886201E-5	0.03967610719858487
>10k+	8.663552773736982E-6	0.10477524304987576
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.273342108433872E-5	3.788903514056453E-6	0.0	0.0	0.0
2	2.84167763554234E-5	3.788903514056453E-6	0.0	3.788903514056453E-6	0.0
3	2.84167763554234E-5	3.788903514056453E-6	0.0	1.3261162299197586E-5	0.0
4	3.2205679869479854E-5	7.577807028112906E-6	0.0	1.7050065813254038E-5	0.0
5	3.2205679869479854E-5	1.3261162299197586E-5	0.0	2.0838969327310492E-5	0.0
6	3.5994583383536304E-5	1.5155614056225813E-5	0.0	2.273342108433872E-5	0.0
7	3.5994583383536304E-5	1.7050065813254038E-5	0.0	2.652232459839517E-5	0.0
8	4.1677938654620984E-5	1.7050065813254038E-5	0.0	3.5994583383536304E-5	0.0
9	4.546684216867744E-5	1.7050065813254038E-5	0.0	3.9783486897592755E-5	0.0
10	3.3342350923696787E-4	2.0838969327310492E-5	0.0	4.546684216867744E-5	0.0
11	3.5994583383536303E-4	2.273342108433872E-5	0.0	5.115019743976212E-5	0.0
12	3.5994583383536303E-4	2.652232459839517E-5	0.0	6.062245622490325E-5	0.0
13	3.67523640863476E-4	3.0311228112451625E-5	0.0	6.441135973895971E-5	0.0
14	3.7510144789158886E-4	3.5994583383536304E-5	0.0	7.009471501004439E-5	0.0
15	3.8457370667672997E-4	3.5994583383536304E-5	0.0	7.577807028112907E-5	0.0
16	3.921515137048429E-4	3.788903514056453E-5	0.0	8.146142555221375E-5	0.0
17	4.01623772489984E-4	3.9783486897592755E-5	0.0	8.714478082329842E-5	0.0
18	4.1677938654620985E-4	4.546684216867744E-5	0.0	9.093368433735488E-5	0.0
19	4.565628734438026E-4	4.736129392570566E-5	0.0	9.661703960843956E-5	0.0
20	4.8119074628516956E-4	4.925574568273389E-5	0.0	1.00405943122496E-4	0.0
21	5.115019743976212E-4	4.925574568273389E-5	0.0	1.0230039487952424E-4	0.0
22	5.304464919679034E-4	4.925574568273389E-5	0.0	1.136671054216936E-4	1.8944517570282266E-6
23	5.531799130522422E-4	4.925574568273389E-5	0.0	1.1556155717872182E-4	3.788903514056453E-6
24	5.986467552209196E-4	5.115019743976212E-5	0.0	1.2503381596386294E-4	3.788903514056453E-6
25	6.479025009036535E-4	5.493910095381857E-5	0.0	1.269282677208912E-4	3.788903514056453E-6
26	6.914748913153027E-4	5.68335527108468E-5	0.0	1.2882271947791942E-4	3.788903514056453E-6
27	7.539917992972342E-4	6.062245622490325E-5	0.0	1.364005265060323E-4	3.788903514056453E-6
28	8.335587730924197E-4	6.820026325301615E-5	0.0	1.3829497826306055E-4	3.788903514056453E-6
29	9.79431558383593E-4	7.009471501004439E-5	0.0	1.4018943002008877E-4	5.68335527108468E-6
30	0.001129093247188823	7.198916676707261E-5	0.0	1.4776723704820166E-4	5.68335527108468E-6
31	0.0013810553308735772	7.388361852410083E-5	0.0	1.5155614056225813E-4	5.68335527108468E-6
32	0.0018584571736446902	7.388361852410083E-5	0.0	1.5534504407631458E-4	5.68335527108468E-6
33	0.004010554369628756	8.146142555221375E-5	0.0	1.6102839934739927E-4	5.68335527108468E-6
34	0.004357239041164921	8.714478082329842E-5	0.0	1.648173028614557E-4	5.68335527108468E-6
35	0.004533423054568546	8.903923258032665E-5	0.0	1.6860620637551216E-4	5.68335527108468E-6
36	0.004652773515261325	9.093368433735488E-5	0.0	1.7239510988956863E-4	5.68335527108468E-6
37	0.004842218690964147	9.472258785141133E-5	0.0	1.7239510988956863E-4	5.68335527108468E-6
38	0.00499945818679749	9.661703960843956E-5	0.0	1.7618401340362507E-4	5.68335527108468E-6
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GATCGGA	3115	0.0	14.766058	1
AAGCAGT	30005	0.0	12.689041	1
TCAACGC	29400	0.0	12.214196	12
ATCAACG	29615	0.0	12.043995	11
CAACGCA	30100	0.0	11.849931	13
GGTATCA	32205	0.0	11.232169	8
GTATCAA	32245	0.0	11.231862	9
GTGGTAT	33000	0.0	11.012617	6
AGCAGTG	34955	0.0	10.915392	2
AACGCAG	33740	0.0	10.662592	14
TGGTATC	34945	0.0	10.456186	7
ACGCAGA	34695	0.0	10.354384	15
CGCAGAG	34570	0.0	10.328344	16
AGTGGTA	36230	0.0	10.200427	5
TATCAAC	36505	0.0	10.029346	10
AGAGTAC	36565	0.0	9.741954	19
GAGTACT	25280	0.0	9.630536	20
TCGTATG	3145	0.0	9.49177	13
CAGAGTA	40665	0.0	8.931743	18
CTCGTAT	3510	0.0	8.754873	12
>>END_MODULE