FastQCFastQC Report
Thu 26 May 2016
SRR522137_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR522137_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48502380
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2528580.5213311181843036No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT919200.18951647321224235No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT344650.018.738291
TCAACGC350700.017.70124212
ATCAACG354200.017.54790911
CAACGCA358500.017.30262213
GTGGTAT372600.016.8151056
GGTATCA372950.016.7943658
AGCAGTG386700.016.5644022
GTATCAA387550.016.2536249
TGGTATC390950.016.0927527
GAGTACT317400.015.67030620
CGCAGAG401250.015.51140716
AACGCAG405150.015.43411414
TATCAAC413950.015.33392610
ACGCAGA414400.015.11455315
AGTGGTA424950.014.8721775
AGAGTAC424950.014.53794919
AGTACTT355250.014.08772921
GTACTTT363800.013.7795922
CAGAGTA457500.013.65699118
GATCGGA26500.013.5483371
Produced by FastQC (version 0.11.5)