FastQCFastQC Report
Thu 26 May 2016
SRR522136_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR522136_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35099636
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT1067710.3041940377957196No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA923400.26307965131034405No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT299200.022.3020861
TCAACGC302200.021.61186412
ATCAACG305150.021.4534411
CAACGCA308550.021.0958213
GGTATCA315850.020.646448
GTGGTAT320150.020.5258546
GTATCAA325150.020.2588339
TGGTATC331100.019.7746457
AGCAGTG337700.019.7021522
GAGTACT269000.019.48022520
AACGCAG336350.019.31231914
TATCAAC341500.019.30822410
CGCAGAG339500.019.0678216
ACGCAGA343150.018.93548615
AGTGGTA354300.018.5660465
AGTACTT288000.018.18743521
GTACTTT289100.018.08780922
AGAGTAC365350.017.77364519
GCAGAGT384650.016.93254917
CAGAGTA389100.016.77306718
Produced by FastQC (version 0.11.5)