##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR522136_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 35099636 Sequences flagged as poor quality 0 Sequence length 50 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 38.6875563040027 40.0 39.0 40.0 36.0 40.0 2 38.54177627369127 40.0 39.0 40.0 36.0 40.0 3 38.52808681548721 40.0 39.0 40.0 36.0 40.0 4 38.51880498133941 40.0 39.0 40.0 36.0 40.0 5 38.5274892879231 40.0 39.0 40.0 36.0 40.0 6 38.56427513949148 40.0 39.0 40.0 36.0 40.0 7 38.52045328903126 40.0 39.0 40.0 35.0 40.0 8 38.494966130133086 40.0 39.0 40.0 35.0 40.0 9 38.473790981764026 40.0 39.0 40.0 35.0 40.0 10 38.449155683551815 40.0 39.0 40.0 35.0 40.0 11 38.48506545765888 40.0 39.0 40.0 35.0 40.0 12 38.442813338577075 40.0 38.0 40.0 35.0 40.0 13 38.4063724193607 40.0 38.0 40.0 35.0 40.0 14 38.38135190347843 40.0 38.0 40.0 35.0 40.0 15 38.357577155500984 40.0 38.0 40.0 35.0 40.0 16 38.38532775667531 40.0 38.0 40.0 35.0 40.0 17 38.3343525271886 40.0 38.0 40.0 35.0 40.0 18 38.29427100041721 40.0 38.0 40.0 35.0 40.0 19 38.25377596508408 40.0 38.0 40.0 35.0 40.0 20 38.21653691793271 40.0 38.0 40.0 35.0 40.0 21 38.1847732267081 40.0 38.0 40.0 35.0 40.0 22 38.126161479281436 40.0 38.0 40.0 35.0 40.0 23 38.06832928409856 40.0 38.0 40.0 35.0 40.0 24 38.0161683443099 40.0 38.0 40.0 35.0 40.0 25 37.96213681532196 40.0 38.0 40.0 34.0 40.0 26 37.843238915640036 39.0 38.0 40.0 34.0 40.0 27 37.742817389901134 39.0 38.0 40.0 33.0 40.0 28 37.652775288040026 39.0 38.0 40.0 33.0 40.0 29 37.588020371493315 39.0 38.0 40.0 33.0 40.0 30 37.51073506859159 39.0 38.0 40.0 33.0 40.0 31 37.40041805561744 39.0 38.0 40.0 33.0 40.0 32 37.28073245545908 39.0 38.0 40.0 33.0 40.0 33 37.207940048153205 39.0 37.0 40.0 33.0 40.0 34 37.36640314446566 39.0 38.0 40.0 33.0 40.0 35 37.30476461351337 39.0 38.0 40.0 33.0 40.0 36 37.376403447602705 39.0 38.0 40.0 33.0 40.0 37 37.29494243188163 39.0 38.0 40.0 33.0 40.0 38 37.356348510280846 39.0 38.0 40.0 33.0 40.0 39 37.302584192041195 39.0 38.0 40.0 33.0 40.0 40 37.23422063408293 39.0 38.0 40.0 33.0 40.0 41 37.23810927839821 39.0 38.0 40.0 33.0 40.0 42 37.14543868774024 39.0 38.0 40.0 33.0 40.0 43 37.08022450717153 39.0 37.0 40.0 33.0 40.0 44 37.00664932821525 39.0 37.0 40.0 33.0 40.0 45 36.9080983631853 39.0 37.0 40.0 33.0 40.0 46 36.487726824289574 39.0 37.0 40.0 32.0 40.0 47 36.642979687880526 39.0 37.0 40.0 32.0 40.0 48 36.574301454294286 39.0 36.0 40.0 32.0 40.0 49 36.47703263931284 39.0 36.0 40.0 31.0 40.0 50 36.38048559819823 39.0 36.0 40.0 31.0 40.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 -0.18207643093934678 1 2 -0.16469916976954835 1 3 -0.17711371251953523 1 4 -0.20098533725420253 1 5 -0.1910561792161687 1 6 -0.2175518966517771 1 7 -0.23448141525338428 1 8 -0.21942086285267948 1 9 -0.2049072341579361 1 10 -0.21765071838565575 1 11 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-0.2646265922219939 120 19 -0.2549168425042794 120 20 -0.29206314010069434 120 21 -0.2453490509012397 120 22 -0.2703898588024529 120 23 -0.2930121761272275 120 24 -0.2639571126431619 120 25 -0.30312492223180953 120 26 -0.26538346880364116 120 27 -0.26492157783597037 120 28 -0.243496949299427 120 29 -0.20731143307146027 120 30 -0.23758390169663102 120 31 -0.23181329697338526 120 32 -0.23367560573207413 120 33 -0.19327730343138683 120 34 -0.24268043597882638 120 35 -0.28119781293201385 120 36 -0.2379612164159468 120 37 -0.256709417263977 120 38 -0.15783883389782716 120 39 -0.14997174048051676 120 40 -0.12841111619120937 120 41 -0.04867200323402443 120 42 -0.04217042553570849 120 43 -0.060653979823491966 120 44 -0.08926284331126055 120 45 -0.045754346382828714 120 46 0.3280730128125313 120 47 0.052104899405627236 120 48 0.0729642399340662 120 49 0.08339583791379823 120 50 0.07038257979227325 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 16962.0 3 1442.0 4 2463.0 5 2773.0 6 3804.0 7 6396.0 8 7615.0 9 8217.0 10 11479.0 11 15573.0 12 17604.0 13 22422.0 14 28075.0 15 32268.0 16 33850.0 17 37442.0 18 41577.0 19 46434.0 20 50457.0 21 52443.0 22 65795.0 23 81289.0 24 97103.0 25 118042.0 26 151703.0 27 187911.0 28 224539.0 29 266865.0 30 319846.0 31 385330.0 32 467139.0 33 588420.0 34 797691.0 35 1151561.0 36 1782171.0 37 3155032.0 38 5532360.0 39 1.9114668E7 40 172875.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 19.99371776809382 29.11038833917379 27.469753391742213 23.426140500990172 2 23.332484466767784 28.495974685385505 26.903913852430815 21.267626995415892 3 23.205977157758838 27.222714443950796 27.014895828009163 22.5564125702812 4 23.785839260555296 26.677299445498527 26.762656456038464 22.77420483790772 5 24.313910263912707 27.76543893503625 26.924319101200933 20.996331699850106 6 24.209795224087223 26.705257000385984 25.818327574679124 23.26662020084767 7 23.587526662669664 26.770798990622012 27.370055917389003 22.27161842931932 8 23.368364845720908 26.793488684612 27.082357777157572 22.75578869250952 9 23.049980858765494 26.513186739331086 27.36870319696081 23.068129204942604 10 22.86942383346407 27.10187809856287 27.100194083453694 22.92850398451937 11 23.03266426657461 27.29718813269265 26.815126956129014 22.855020644603723 12 22.870442303162346 26.85737253799441 27.250516216179566 23.021668942663677 13 22.84820870879089 26.982730863748568 26.96929720180668 23.199763225653864 14 22.85603504818088 27.301019807382477 27.061630422825704 22.78131472161094 15 22.781863036984202 27.144691756917368 27.008527951685878 23.064917254412553 16 22.886183207142093 26.748627820527826 27.060594018695806 23.30459495363428 17 23.373521594354997 26.714371055016066 27.080175418343373 22.831931932285563 18 22.912964111650616 26.56577122338249 27.04040577514821 23.480858889818688 19 22.992449266425442 27.011237381493075 27.065263582790433 22.931049769291054 20 23.42772158662842 26.522531458730796 27.11343787154944 22.93630908309135 21 22.96948321629318 26.90884885529867 27.023701898219116 23.09796603018903 22 23.422812703812653 26.586301350817426 26.906714930035168 23.084171015334746 23 22.97739213022038 26.656179568357917 27.347420355014506 23.019007946407193 24 22.96402048955073 26.906542985630427 26.897457171526522 23.231979353292324 25 23.059108038062384 26.608618924435795 26.892709258848512 23.43956377865331 26 23.226335765869354 26.666256975189707 27.218377494684198 22.889029764256744 27 23.19927477310591 26.477753216586063 27.19355836054824 23.129413649759787 28 23.30149805542143 26.644296254240356 27.015237422974987 23.038968267363227 29 23.288839804492557 26.681347920531138 27.10105312773044 22.92875914724586 30 23.219209452770393 26.521021471561703 27.09741206433024 23.16235701133767 31 23.2576542959021 26.629811773546596 27.03400115032532 23.078532780225984 32 23.2241496749425 26.65252425979574 27.064628248566454 23.058697816695307 33 23.19266216891822 26.594401149915058 26.963102979187592 23.24983370197913 34 23.17081009045222 26.75354240140838 26.987279868087523 23.088367640051878 35 23.203026948769498 26.73283563396498 26.972131562845835 23.092005854419686 36 23.113117184463107 26.645566922688314 27.010596349204306 23.23071954364427 37 23.21555984227301 26.735596346355273 26.911105288955135 23.137738522416587 38 23.227169649280693 26.69729965290808 26.989826903048225 23.085703794763 39 23.187280346724965 26.592207394971272 26.96570414576379 23.25480811253997 40 23.197519769150883 26.697296803875687 26.94002011872716 23.16516330824627 41 23.212237870500992 26.748946912156015 26.95355017356875 23.085265043774243 42 23.172251700843848 26.653569854684534 26.907680752016915 23.266497692454703 43 23.257777084254705 26.726961957635194 26.853291864021827 23.16196909408827 44 23.175643530725605 26.73999181275597 26.935468252073587 23.148896404444834 45 23.134947643284654 26.649314543204977 26.903030449274418 23.312707364235948 46 23.230004993243384 26.865290286336364 26.64059576337292 23.26410895704733 47 23.240491997684877 26.769983203463365 26.818211616970984 23.171313181880773 48 23.175326457900024 26.64269330016664 26.822761592552176 23.359218649381162 49 23.19773499998161 26.743091381293794 26.763214602810002 23.295959015914594 50 23.14484391725486 26.78794797410059 26.85254850906807 23.214659599576482 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 102206.0 1 57885.5 2 13565.0 3 10773.0 4 7981.0 5 7983.0 6 7985.0 7 8908.0 8 9831.0 9 11549.0 10 13267.0 11 17992.5 12 22718.0 13 31461.0 14 40204.0 15 54859.5 16 69515.0 17 99257.0 18 128999.0 19 175356.5 20 221714.0 21 289481.5 22 357249.0 23 478255.5 24 599262.0 25 604271.5 26 609281.0 27 689342.5 28 769404.0 29 847431.5 30 925459.0 31 1029203.0 32 1132947.0 33 1234897.5 34 1336848.0 35 1452819.0 36 1568790.0 37 1660261.0 38 1751732.0 39 1829460.0 40 1907188.0 41 1979744.0 42 2052300.0 43 2095250.0 44 2138200.0 45 2151342.0 46 2164484.0 47 2142400.0 48 2120316.0 49 2124939.5 50 2129563.0 51 2123513.0 52 2117463.0 53 2088760.5 54 2060058.0 55 1977656.5 56 1895255.0 57 1798172.5 58 1701090.0 59 1581053.0 60 1461016.0 61 1305377.0 62 1149738.0 63 997505.0 64 845272.0 65 735555.0 66 625838.0 67 524793.0 68 423748.0 69 345124.0 70 266500.0 71 216406.5 72 166313.0 73 129115.0 74 91917.0 75 68816.5 76 45716.0 77 34771.0 78 23826.0 79 17556.0 80 11286.0 81 8375.0 82 5464.0 83 4478.5 84 3493.0 85 2928.0 86 2363.0 87 1825.0 88 1287.0 89 878.5 90 470.0 91 355.5 92 241.0 93 179.5 94 118.0 95 92.5 96 67.0 97 56.0 98 45.0 99 44.5 100 44.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.09253942120653331 2 0.027658406486038775 3 7.920310056776657E-4 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 5.4131615495955575E-5 10 0.014071370996553922 11 4.2450582678407267E-4 12 0.0 13 0.0035299511368152084 14 0.002145321393076555 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0027493162607156382 25 0.0026809394832470624 26 1.509987169097708E-4 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 7.60691649337902E-4 44 0.08674733834846605 45 0.1138188441612329 46 0.3372257193778306 47 0.08859636037251213 48 0.08751942612738206 49 0.0733027544787074 50 0.0818213613383341 >>END_MODULE >>Sequence Length Distribution pass #Length Count 50 3.5099636E7 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.07244102887214 #Duplication Level Percentage of deduplicated Percentage of total 1 85.1083269519237 42.615816823661696 2 7.424928935075188 7.435686324902376 3 2.458188278065006 3.692624627738243 4 1.191423009305287 2.3862983349552147 5 0.6988308673636529 1.7496083697611038 6 0.4795286733398785 1.4406702730478613 7 0.3220728381495279 1.1288881236670605 8 0.24144095884034103 0.9671630538789857 9 0.19369813833817268 0.8729044748406422 >10 1.4852233993140138 15.53904444988824 >50 0.24964418122090584 8.762981547678628 >100 0.14494896348507347 12.163544608632845 >500 0.0014936211460086514 0.46574450286419433 >1k 2.284141235481289E-4 0.1597524156312844 >5k 1.1385154959527108E-5 0.051723585253598194 >10k+ 1.1385154959455732E-5 0.5675484835980319 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 106771 0.3041940377957196 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 92340 0.26307965131034405 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 8.547097183571931E-6 2.8490323945239773E-6 0.0 0.0 0.0 2 8.547097183571931E-6 5.6980647890479545E-6 0.0 5.6980647890479545E-6 0.0 3 8.547097183571931E-6 1.1396129578095909E-5 0.0 5.6980647890479545E-6 0.0 4 8.547097183571931E-6 1.1396129578095909E-5 0.0 1.1396129578095909E-5 0.0 5 8.547097183571931E-6 1.1396129578095909E-5 0.0 1.1396129578095909E-5 0.0 6 1.1396129578095909E-5 1.1396129578095909E-5 0.0 1.1396129578095909E-5 0.0 7 1.7094194367143863E-5 1.1396129578095909E-5 0.0 1.1396129578095909E-5 0.0 8 1.7094194367143863E-5 1.4245161972619887E-5 0.0 1.4245161972619887E-5 0.0 9 1.9943226761667842E-5 1.7094194367143863E-5 0.0 1.4245161972619887E-5 0.0 10 6.837677746857545E-5 1.9943226761667842E-5 0.0 1.4245161972619887E-5 0.0 11 7.122580986309943E-5 2.2792259156191818E-5 0.0 1.7094194367143863E-5 0.0 12 7.407484225762341E-5 2.5641291550715798E-5 0.0 1.9943226761667842E-5 0.0 13 7.407484225762341E-5 2.8490323945239774E-5 0.0 2.5641291550715798E-5 0.0 14 7.692387465214739E-5 3.133935633976375E-5 0.0 2.5641291550715798E-5 2.8490323945239773E-6 15 7.977290704667137E-5 3.4188388734287726E-5 0.0 3.4188388734287726E-5 2.8490323945239773E-6 16 8.262193944119535E-5 3.7037421128811705E-5 0.0 3.4188388734287726E-5 2.8490323945239773E-6 17 8.547097183571933E-5 3.9886453523335684E-5 0.0 4.2735485917859664E-5 2.8490323945239773E-6 18 9.401806901929125E-5 3.9886453523335684E-5 0.0 4.5584518312383636E-5 2.8490323945239773E-6 19 1.1965936057000705E-4 3.9886453523335684E-5 0.0 4.8433550706907616E-5 2.8490323945239773E-6 20 1.3390452254262694E-4 3.9886453523335684E-5 0.0 5.1282583101431595E-5 2.8490323945239773E-6 21 1.652438788823907E-4 3.9886453523335684E-5 0.0 5.1282583101431595E-5 2.8490323945239773E-6 22 1.908851704331065E-4 3.9886453523335684E-5 0.0 5.413161549595557E-5 2.8490323945239773E-6 23 2.2507355916739422E-4 3.9886453523335684E-5 0.0 5.413161549595557E-5 2.8490323945239773E-6 24 2.7065807747977787E-4 3.9886453523335684E-5 0.0 5.9829680285003527E-5 2.8490323945239773E-6 25 3.361858225538293E-4 3.9886453523335684E-5 0.0 5.9829680285003527E-5 2.8490323945239773E-6 26 3.73223243682641E-4 3.9886453523335684E-5 0.0 6.26787126795275E-5 2.8490323945239773E-6 27 3.9886453523335684E-4 3.9886453523335684E-5 0.0 6.552774507405149E-5 2.8490323945239773E-6 28 4.6154324791288436E-4 3.9886453523335684E-5 0.0 7.407484225762341E-5 2.8490323945239773E-6 29 5.299200253814598E-4 3.9886453523335684E-5 0.0 7.692387465214739E-5 2.8490323945239773E-6 30 6.210890620062271E-4 3.9886453523335684E-5 0.0 7.977290704667137E-5 2.8490323945239773E-6 31 7.720877789159979E-4 4.5584518312383636E-5 0.0 8.547097183571933E-5 2.8490323945239773E-6 32 0.001008557467661488 4.8433550706907616E-5 0.0 8.547097183571933E-5 2.8490323945239773E-6 33 0.0015698168493827117 4.8433550706907616E-5 0.0 8.83200042302433E-5 2.8490323945239773E-6 34 0.0017464568578431981 4.8433550706907616E-5 0.0 9.971613380833921E-5 2.8490323945239773E-6 35 0.0018604181536241572 4.8433550706907616E-5 0.0 1.0541419859738717E-4 2.8490323945239773E-6 36 0.002042756226873692 4.8433550706907616E-5 0.0 1.1111226338643511E-4 2.8490323945239773E-6 37 0.002236490429701322 4.8433550706907616E-5 0.0 1.1111226338643511E-4 2.8490323945239773E-6 38 0.0024615639888687165 4.8433550706907616E-5 0.0 1.1111226338643511E-4 2.8490323945239773E-6 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AAGCAGT 29920 0.0 22.302086 1 TCAACGC 30220 0.0 21.611864 12 ATCAACG 30515 0.0 21.45344 11 CAACGCA 30855 0.0 21.09582 13 GGTATCA 31585 0.0 20.64644 8 GTGGTAT 32015 0.0 20.525854 6 GTATCAA 32515 0.0 20.258833 9 TGGTATC 33110 0.0 19.774645 7 AGCAGTG 33770 0.0 19.702152 2 GAGTACT 26900 0.0 19.480225 20 AACGCAG 33635 0.0 19.312319 14 TATCAAC 34150 0.0 19.308224 10 CGCAGAG 33950 0.0 19.06782 16 ACGCAGA 34315 0.0 18.935486 15 AGTGGTA 35430 0.0 18.566046 5 AGTACTT 28800 0.0 18.187435 21 GTACTTT 28910 0.0 18.087809 22 AGAGTAC 36535 0.0 17.773645 19 GCAGAGT 38465 0.0 16.932549 17 CAGAGTA 38910 0.0 16.773067 18 >>END_MODULE