FastQCFastQC Report
Thu 26 May 2016
SRR522135_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR522135_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29743480
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT314070.1055928896013513No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG25000.022.22600713
TATGCCG26800.020.97828716
CGTATGC27550.020.48690614
CTCGTAT28950.019.3451712
GATCGGA37650.018.1609251
ATCGGAA44500.015.4992942
TAGATCG15200.014.89327132
GAGTACT171500.012.8592920
GTACTTT168950.012.65173922
ATGCCGT48200.012.16539417
TCGGAAG58150.012.0653513
AGTACTT184500.011.79909621
GCTCGTA49500.011.713428511
CCGTCTT48950.011.66723720
AGAGCGG36150.011.43765241
AGCTCGT50250.011.276360510
GCCGTCT50850.011.2734319
GAGCGGT34450.011.17226642
GAGTACA187400.010.875277517
AGTACAT202150.010.15812818
Produced by FastQC (version 0.11.5)