##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR522135_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	29743480
Sequences flagged as poor quality	0
Sequence length	50
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	38.79681543652592	40.0	39.0	40.0	37.0	40.0
2	38.65809252985864	40.0	39.0	40.0	36.0	40.0
3	38.653545079459434	40.0	39.0	40.0	36.0	40.0
4	38.649183148710236	40.0	39.0	40.0	36.0	40.0
5	38.64594385055145	40.0	39.0	40.0	36.0	40.0
6	38.61087055045341	40.0	39.0	40.0	36.0	40.0
7	38.55902846607055	40.0	39.0	40.0	36.0	40.0
8	38.54304015535506	40.0	39.0	40.0	36.0	40.0
9	38.12893037398448	40.0	39.0	40.0	35.0	40.0
10	38.46699303511223	40.0	39.0	40.0	35.0	40.0
11	38.44787260939204	40.0	39.0	40.0	35.0	40.0
12	38.401868812929756	40.0	38.0	40.0	35.0	40.0
13	38.37170041972224	40.0	38.0	40.0	35.0	40.0
14	38.32790581330766	40.0	38.0	40.0	35.0	40.0
15	38.2821407918643	40.0	38.0	40.0	35.0	40.0
16	38.341316449857246	40.0	38.0	40.0	35.0	40.0
17	38.26853441493732	40.0	38.0	40.0	35.0	40.0
18	38.19988189008146	40.0	38.0	40.0	35.0	40.0
19	38.14396644239343	40.0	38.0	40.0	35.0	40.0
20	38.079440603453264	40.0	38.0	40.0	35.0	40.0
21	38.248773680820136	40.0	38.0	40.0	35.0	40.0
22	38.18545284546395	40.0	38.0	40.0	35.0	40.0
23	38.12405727238373	40.0	38.0	40.0	35.0	40.0
24	38.07577092525824	40.0	38.0	40.0	35.0	40.0
25	38.03516189094215	40.0	38.0	40.0	35.0	40.0
26	37.93128036799998	40.0	38.0	40.0	35.0	40.0
27	37.8568804995246	40.0	38.0	40.0	35.0	40.0
28	37.79849698152334	39.0	38.0	40.0	34.0	40.0
29	37.7322889587903	39.0	38.0	40.0	34.0	40.0
30	37.735567795026	40.0	38.0	40.0	34.0	40.0
31	37.68266860501865	40.0	38.0	40.0	34.0	40.0
32	37.56916564571463	40.0	38.0	40.0	33.0	40.0
33	37.511359867776065	39.0	38.0	40.0	33.0	40.0
34	37.4503172123773	39.0	38.0	40.0	33.0	40.0
35	37.318290260588206	39.0	38.0	40.0	33.0	40.0
36	37.25486392311861	39.0	38.0	40.0	33.0	40.0
37	37.1563692278106	39.0	38.0	40.0	33.0	40.0
38	37.090738407207226	39.0	38.0	40.0	33.0	40.0
39	36.98336838863509	39.0	37.0	40.0	33.0	40.0
40	36.88845491516124	39.0	37.0	40.0	33.0	40.0
41	36.79633338129903	39.0	37.0	40.0	33.0	40.0
42	36.67704206098278	39.0	37.0	40.0	32.0	40.0
43	36.55434666689977	39.0	37.0	40.0	32.0	40.0
44	36.44833032314981	39.0	36.0	40.0	31.0	40.0
45	36.33076331350602	39.0	36.0	40.0	31.0	40.0
46	36.16966884843334	39.0	36.0	40.0	31.0	40.0
47	36.06669179262144	39.0	36.0	40.0	31.0	40.0
48	35.948543512729515	39.0	36.0	40.0	30.0	40.0
49	35.82765627962834	39.0	36.0	40.0	30.0	40.0
50	35.723306654096966	39.0	36.0	40.0	30.0	40.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1	1	-0.2688873940450591
1	2	-0.3239673815550006
1	3	-0.3663783099330544
1	4	-0.33940919313631923
1	5	-0.28021528990556277
1	6	-0.3613507472509596
1	7	-0.8892246325974469
1	8	-0.9426815903863073
1	9	-0.438451874540398
1	10	-0.7747885364257883
1	11	-1.022368195103013
1	12	-0.8517641419966964
1	13	-1.0063630902573948
1	14	-0.8564983898980643
1	15	-0.6314363974309032
1	16	-0.29010236892101204
1	17	-0.3216617549896412
1	18	-0.28827442322108965
1	19	-0.3389712822878792
1	20	-0.2904426727408591
1	21	-0.31348685941016896
1	22	-0.3303741189580194
1	23	-0.3667085929703262
1	24	-0.36992657901965487
1	25	-0.33363005260996204
1	26	-0.4387635645137422
1	27	-0.35699325588167596
1	28	-0.3601061709660627
1	29	-0.38158864461569664
1	30	-0.3321696145905264
1	31	-0.2840827620909607
1	32	-0.28908031833191217
1	33	-0.37212989859248324
1	34	-0.2661368104892645
1	35	-0.25889300501231816
1	36	-0.2514653201055097
1	37	-0.21155240134747544
1	38	-0.24356271045493116
1	39	-0.22273495655712594
1	40	-0.22594672688123296
1	41	-0.2275875986811684
1	42	-0.20765252724859806
1	43	-0.2596479695046128
1	44	-0.26381387655595745
1	45	-0.2083383450864531
1	46	-0.24525854271339398
1	47	-0.19897832652753067
1	48	-0.18649055687670568
1	49	-0.20299974624271755
1	50	-0.22614883808883235
2	1	-0.47897830475633896
2	2	-0.5902338770982141
2	3	-0.5612805674442569
2	4	-0.4782833019263819
2	5	-0.5220255627955837
2	6	-0.5088131962381865
2	7	-0.4936079790849419
2	8	-0.47563077081189675
2	9	-0.015323680731846423
2	10	-0.5646005831901562
2	11	-0.4504451248269916
2	12	-0.33925576355508014
2	13	-0.35461682759602553
2	14	-0.33598456866428705
2	15	-0.3063708598687498
2	16	-0.26276773743727944
2	17	-0.27087434797619636
2	18	-0.2810130708506904
2	19	-0.2765130387140644
2	20	-0.2710546349850773
2	21	-0.2693993197147009
2	22	-0.2692134100564587
2	23	-0.2641624308018038
2	24	-0.3014026108104346
2	25	-0.32409343951575664
2	26	-0.2821987071330625
2	27	-0.3604524370780595
2	28	-0.36475298377438037
2	29	-0.39414599529692396
2	30	-0.37653755194711636
2	31	-0.3270615926211491
2	32	-0.3646865296644535
2	33	-0.48591820906591465
2	34	-0.31905126861263966
2	35	-0.3749512189327646
2	36	-0.3536987552555786
2	37	-0.3086270972866956
2	38	-0.3108692490326135
2	39	-0.3134370900771728
2	40	-0.29446487427030377
2	41	-0.23457591283948176
2	42	-0.22320519742355316
2	43	-0.24530663727533408
2	44	-0.21444648577563896
2	45	-0.21641641199958883
2	46	-0.20080555911135178
2	47	-0.22338918476057046
2	48	-0.26197310405431296
2	49	-0.25625685227105777
2	50	-0.3230396022545108
3	1	-0.3842457149306284
3	2	-0.4529533990418386
3	3	-0.5149217546979656
3	4	-0.4710905671201857
3	5	-0.48880881921248687
3	6	-0.45459806409923686
3	7	-0.4289689494237763
3	8	-0.428118827776764
3	9	0.01372139177651377
3	10	-0.43112405550704125
3	11	-0.3275392607859402
3	12	-0.2697021967478648
3	13	-0.2756078361202512
3	14	-0.2627475484727029
3	15	-0.2820956557583685
3	16	-0.3030503879078097
3	17	-0.30039967287537195
3	18	-0.305552151500585
3	19	-0.2675816894461889
3	20	-0.32363417756013035
3	21	-0.34010482603589764
3	22	-0.33310767173350087
3	23	-0.323292929054638
3	24	-0.3448985443531214
3	25	-0.3289439936534393
3	26	-0.30935398945010206
3	27	-0.32621649141453446
3	28	-0.30691378728052854
3	29	-0.37849510629727234
3	30	-0.41725638227651274
3	31	-0.3800302330094709
3	32	-0.4630103730766919
3	33	-0.5458617001867268
3	34	-0.46280191402578197
3	35	-0.3905161323244357
3	36	-0.4234019077822424
3	37	-0.3765451105720885
3	38	-0.3573436071405709
3	39	-0.3325866100641903
3	40	-0.382460750313399
3	41	-0.24174056532088173
3	42	-0.21417753678831275
3	43	-0.24618834714019755
3	44	-0.21462347317907415
3	45	-0.22538482084250688
3	46	-0.233645782180659
3	47	-0.232591113008489
3	48	-0.2630268483091598
3	49	-0.2617456783655925
3	50	-0.3230242289214047
4	1	-0.5097659738308948
4	2	-0.5117620663947591
4	3	-0.5448953412698287
4	4	-0.4866721837600778
4	5	-0.5194156574020994
4	6	-0.4760125269041211
4	7	-0.4347685041664633
4	8	-0.43916529797699155
4	9	0.0700624366042959
4	10	-0.4306991080102236
4	11	-0.30972327651350184
4	12	-0.29664659048142283
4	13	-0.3081619887366216
4	14	-0.2673494270959509
4	15	-0.2427960717215214
4	16	-0.2905774459289532
4	17	-0.31249799598225536
4	18	-0.2831442817539198
4	19	-0.29113880203609455
4	20	-0.3104146273195525
4	21	-0.32905913742274606
4	22	-0.3533727451475741
4	23	-0.3302905493145971
4	24	-0.3930511665806833
4	25	-0.37358421102084094
4	26	-0.3036500018712829
4	27	-0.3710742007380716
4	28	-0.353544250536153
4	29	-0.3775779040952827
4	30	-0.34861067814551205
4	31	-0.3693443577760007
4	32	-0.4616721396874013
4	33	-0.4262839092257451
4	34	-0.47804890253379995
4	35	-0.4454135941994082
4	36	-0.2708139946796635
4	37	-0.28620494146238684
4	38	-0.33271999805188557
4	39	-0.2852420539648506
4	40	-0.34985226622981
4	41	-0.1992248244433128
4	42	-0.22959221619889547
4	43	-0.25827418858063567
4	44	-0.21655099670454803
4	45	-0.21169254859517395
4	46	-0.20414676830209544
4	47	-0.19210608608562296
4	48	-0.16518059746805136
4	49	-0.19741358679640086
4	50	-0.28097538024843516
5	1	-0.39748465690846757
5	2	-0.442190926814078
5	3	-0.42612617120709473
5	4	-0.426275333335731
5	5	-0.3822327575782012
5	6	-0.32384682282754085
5	7	-0.3523540295021377
5	8	-0.29071818053610343
5	9	0.12194397793529532
5	10	-0.34595971646349
5	11	-0.24823672748556902
5	12	-0.2380199585903
5	13	-0.24242187251667957
5	14	-0.23062552045790596
5	15	-0.20895307054028223
5	16	-0.2710546346389222
5	17	-0.2607431724950118
5	18	-0.26623663877298753
5	19	-0.27288850736736947
5	20	-0.3060127433228601
5	21	-0.254717316265733
5	22	-0.29179585080789394
5	23	-0.2924088871554815
5	24	-0.3011545412366132
5	25	-0.30836382903184045
5	26	-0.3446953750340569
5	27	-0.3435821115421831
5	28	-0.36559393953179864
5	29	-0.3817485718553755
5	30	-0.37101682760705046
5	31	-0.2940718902817494
5	32	-0.34061354870906513
5	33	-0.2876529039636182
5	34	-0.2892674343595445
5	35	-0.2780820019281407
5	36	-0.17750844340789484
5	37	-0.2784422079294018
5	38	-0.2110345250560144
5	39	-0.16051930218670662
5	40	-0.16179213243758994
5	41	-0.10597923417207511
5	42	-0.13916926442008304
5	43	-0.17311870432867948
5	44	-0.19205760757228774
5	45	-0.12267550893555779
5	46	-0.1699493294523151
5	47	-0.2264876528764148
5	48	-0.22591790976269266
5	49	-0.1856127339831417
5	50	-0.2669027264653536
6	1	-0.2876697946069626
6	2	-0.322810679669594
6	3	-0.3176532695008092
6	4	-0.30162429199205576
6	5	-0.2944135645221664
6	6	-0.2877546795609902
6	7	-0.3051883577775314
6	8	-0.3163495492875441
6	9	0.1397937479517708
6	10	-0.3062926274307074
6	11	-0.29462925210202684
6	12	-0.17262036850632967
6	13	-0.21172046993857663
6	14	-0.2115628553565827
6	15	-0.19467998324644498
6	16	-0.21274026568129045
6	17	-0.21508572953040073
6	18	-0.2206685298601414
6	19	-0.23109999287804328
6	20	-0.2423655606051156
6	21	-0.2373944769493832
6	22	-0.24885351478034323
6	23	-0.24748610295628026
6	24	-0.28104107143773405
6	25	-0.269271468220019
6	26	-0.2978001491240079
6	27	-0.3077419376421986
6	28	-0.3345426991760547
6	29	-0.338199118478677
6	30	-0.31658197915295005
6	31	-0.2956205004399024
6	32	-0.35643513547266537
6	33	-0.29145977314369986
6	34	-0.3588103352668952
6	35	-0.38418976503056257
6	36	-0.30045465920571246
6	37	-0.3090081307463848
6	38	-0.28656674680347294
6	39	-0.3087105643594157
6	40	-0.29041325442344146
6	41	-0.24180270547660854
6	42	-0.24539242159661967
6	43	-0.2233979072984269
6	44	-0.2471947966177126
6	45	-0.2358595728146895
6	46	-0.2147673314728067
6	47	-0.2604199618122749
6	48	-0.24300518146255
6	49	-0.2199364938580075
6	50	-0.25465113854804855
7	1	-0.276634825111465
7	2	-0.4157891859677818
7	3	-0.3611346078991673
7	4	-0.32075169509359824
7	5	-0.37920587574343045
7	6	-0.2741300469517327
7	7	-0.3005144282575287
7	8	-0.3272039933508779
7	9	0.15811100735793104
7	10	-0.2354803231611129
7	11	-0.18302497129958795
7	12	-0.16683544249453774
7	13	-0.15956357499893414
7	14	-0.1421484540691793
7	15	-0.13950726768833022
7	16	-0.17671657042885158
7	17	-0.1990175935013383
7	18	-0.21337876103871878
7	19	-0.2119146554401965
7	20	-0.19031406959399533
7	21	-0.21695116952861326
7	22	-0.20149132947127413
7	23	-0.20829538127577507
7	24	-0.23007063548600826
7	25	-0.24269591818338654
7	26	-0.24743008631548236
7	27	-0.26003738155279876
7	28	-0.2546400281733412
7	29	-0.305067904857772
7	30	-0.32238447057584096
7	31	-0.3195790707128694
7	32	-0.2937914780617916
7	33	-0.37197384080290874
7	34	-0.2906530213936307
7	35	-0.37400347997746763
7	36	-0.24710122737426587
7	37	-0.27375830790487754
7	38	-0.24375973106939597
7	39	-0.2531705855697197
7	40	-0.25058945584168413
7	41	-0.12652298418495178
7	42	-0.15726718394061834
7	43	-0.1336927912936403
7	44	-0.1432744203683285
7	45	-0.11676619732436677
7	46	-0.11033629175807391
7	47	-0.1426887968472741
7	48	-0.14263814746961145
7	49	-0.16717460948196106
7	50	-0.2260457233008708
8	1	-0.30088474481758
8	2	-0.4013307087113276
8	3	-0.36432192079652026
8	4	-0.3696063377146004
8	5	-0.38941798859108445
8	6	-0.32680890458815526
8	7	-0.3384182257831654
8	8	-0.3148339873150263
8	9	0.16983960942135212
8	10	-0.32411327319592687
8	11	-0.2002930857107259
8	12	-0.1910672639676534
8	13	-0.20001258087273044
8	14	-0.16001985588746948
8	15	-0.1682310965406515
8	16	-0.215657273456074
8	17	-0.2432571088641282
8	18	-0.23336036493085288
8	19	-0.2101521340447121
8	20	-0.24231151254070937
8	21	-0.24071198286605267
8	22	-0.2523274756203051
8	23	-0.2664198610002302
8	24	-0.27728059631753865
8	25	-0.25305324502532756
8	26	-0.253701857062687
8	27	-0.27972870016644436
8	28	-0.3355495345692674
8	29	-0.31630359927491014
8	30	-0.3967367471415457
8	31	-0.2799351832217596
8	32	-0.33869155946881335
8	33	-0.31894731546177724
8	34	-0.37977079649468237
8	35	-0.31535188110820656
8	36	-0.3282411197355728
8	37	-0.4473520570372642
8	38	-0.2892532399143306
8	39	-0.267377919030821
8	40	-0.25525250773984
8	41	-0.19387808168784915
8	42	-0.17900426453969231
8	43	-0.20788220970958093
8	44	-0.18422513001885932
8	45	-0.15992544926822205
8	46	-0.13978936912452866
8	47	-0.17560766637377867
8	48	-0.18756304745708263
8	49	-0.1665146791462817
8	50	-0.2155288076673827
9	1	-0.32808402077184695
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114	25	-0.2771969992872485
114	26	-0.34823328476772275
114	27	-0.3051033059794577
114	28	-0.32394893881451736
114	29	-0.36550957903003933
114	30	-0.45910926587361445
114	31	-0.2921910700461865
114	32	-0.3033294727001845
114	33	-0.29687244282549585
114	34	-0.3169296528954604
114	35	-0.42932466627549815
114	36	-0.30958190606505553
114	37	-0.2572416456694242
114	38	-0.26291867584104267
114	39	-0.3968251046662843
114	40	-0.30335037075905547
114	41	-0.20916944084169842
114	42	-0.27670591548399415
114	43	-0.37159974376968563
114	44	-0.2546945682820976
114	45	-0.3620546308947752
114	46	-0.27000954168296687
114	47	-0.34078486775449335
114	48	-0.3242340504139065
114	49	-0.31406185822092425
114	50	-0.3260134572950193
115	1	-0.31558768292234873
115	2	-0.31890398430737577
115	3	-0.2890581174345712
115	4	-0.2648872658662498
115	5	-0.2629092299271889
115	6	-0.19768549776417643
115	7	-0.2481900735986997
115	8	-0.22261795495327164
115	9	0.22890221065200933
115	10	-0.19646627866718092
115	11	-0.15189731009931506
115	12	-0.18359938594183234
115	13	-0.16046282510370702
115	14	-0.1633859047888393
115	15	-0.2291178230853106
115	16	-0.2590480268513673
115	17	-0.25791860798580046
115	18	-0.2681288330213931
115	19	-0.25454275497008183
115	20	-0.2703777416685895
115	21	-0.34821508707230464
115	22	-0.3015636676840785
115	23	-0.5106580777833969
115	24	-0.4049315094295878
115	25	-0.4088560536993029
115	26	-0.4366537863411395
115	27	-0.4160931306235156
115	28	-0.37619919149708636
115	29	-0.393116642234844
115	30	-0.4349654616646603
115	31	-0.2894729201137167
115	32	-0.31294483992633815
115	33	-0.31127329899432254
115	34	-0.2800395472981876
115	35	-0.33146490307745324
115	36	-0.3581258859122798
115	37	-0.33326812480646595
115	38	-0.5506976812714939
115	39	-0.6855026966367035
115	40	-0.6103847090147028
115	41	-0.8952077729904389
115	42	-0.9632448440855583
115	43	-0.7692946877760676
115	44	-1.3149024229431134
115	45	-1.2358924535221831
115	46	-1.1297697172946926
115	47	-1.0176875693889116
115	48	-0.9752157897134097
115	49	-1.2615651586282155
115	50	-0.8705605589551979
116	1	-0.3026682701237604
116	2	-0.34175021706115416
116	3	-0.3108071524397502
116	4	-0.3260311717440274
116	5	-0.2700434007445267
116	6	-0.28636005023506783
116	7	-0.24475726602290138
116	8	-0.2540246963600552
116	9	0.18004241523250641
116	10	-0.2458752720720696
116	11	-0.21129028085538692
116	12	-0.21540468462316653
116	13	-0.21707792113104318
116	14	-0.2023377742380319
116	15	-0.17819142970819968
116	16	-0.24792366903386664
116	17	-0.24836285490069088
116	18	-0.23411716761405188
116	19	-0.24869772829836734
116	20	-0.2544598795783557
116	21	-0.2843105597657285
116	22	-0.29966521382489475
116	23	-0.3363619329972636
116	24	-0.318445534482656
116	25	-0.32756642202015485
116	26	-0.30566904998766375
116	27	-0.32448955704132487
116	28	-0.3393823395905855
116	29	-0.3609512118348164
116	30	-0.30171688789256734
116	31	-0.261310513630697
116	32	-0.281283569748183
116	33	-0.3341452385603958
116	34	-0.3497264694342519
116	35	-0.3825922350142932
116	36	-0.2760244147427997
116	37	-0.27122508235768095
116	38	-0.30562997594773833
116	39	-0.31344872233439247
116	40	-0.2702007896069105
116	41	-0.1854640968964958
116	42	-0.20291486359404587
116	43	-0.23105896493672873
116	44	-0.33041020448918346
116	45	-0.24231593568661935
116	46	-0.31181400633423806
116	47	-0.3446275996335757
116	48	-0.3719663183739783
116	49	-0.3355201783573776
116	50	-0.5156664122649914
117	1	-0.25686292052032655
117	2	-0.38182689998846797
117	3	-0.3821098099110998
117	4	-0.32822140328760696
117	5	-0.3439547069334381
117	6	-0.29080353162654404
117	7	-0.27922305626479726
117	8	-0.25120849825677993
117	9	0.19611032346942636
117	10	-0.2580030140480005
117	11	-0.22378175654698396
117	12	-0.20450747854341245
117	13	-0.1712273376805129
117	14	-0.1869602722589221
117	15	-0.17925380368095745
117	16	-0.29665764994294364
117	17	-0.23210377396944182
117	18	-0.21392963499609863
117	19	-0.27690856412095144
117	20	-0.23122804990584456
117	21	-0.3112003947235351
117	22	-0.34490126046613057
117	23	-0.3168364128312575
117	24	-0.4072296088519849
117	25	-0.3096982470076526
117	26	-0.36504062887305366
117	27	-0.3410314871590572
117	28	-0.3234987369307518
117	29	-0.3661488455202502
117	30	-0.28672839945148354
117	31	-0.26168809060433773
117	32	-0.33359248004117603
117	33	-0.24371639489743302
117	34	-0.3542954096098754
117	35	-0.3278549804186426
117	36	-0.28415148923329525
117	37	-0.21163606293021076
117	38	-0.2701550942395272
117	39	-0.26435874020086203
117	40	-0.2854154527346324
117	41	-0.15925468028177647
117	42	-0.18366525326921845
117	43	-0.1910831082981943
117	44	-0.19148779579750652
117	45	-0.21509936686095443
117	46	-0.28317873387808845
117	47	-0.2500270347136322
117	48	-0.3249082384168531
117	49	-0.27996797587990585
117	50	-0.4222342602923277
118	1	-0.26396956059313226
118	2	-0.2909697526292092
118	3	-0.2887712031619074
118	4	-0.29364527458452017
118	5	-0.27093030918076266
118	6	-0.18867153730771236
118	7	-0.2164142674925671
118	8	-0.2886164079300855
118	9	0.24989691146544857
118	10	-0.19573783499632214
118	11	-0.17839425652960728
118	12	-0.22023861349181573
118	13	-0.19100555487538173
118	14	-0.1500859236252694
118	15	-0.18697415238042936
118	16	-0.2270321087195626
118	17	-0.2492155330864847
118	18	-0.2512968946001948
118	19	-0.23091665425624797
118	20	-0.24635840426023492
118	21	-0.2785853720963942
118	22	-0.34336229221872117
118	23	-0.3163672271247293
118	24	-0.32427191641036046
118	25	-0.3490777532716862
118	26	-0.32620222319949477
118	27	-0.380770269233345
118	28	-0.3481878743260225
118	29	-0.35643742575947357
118	30	-0.2852311716706737
118	31	-0.6544793120475276
118	32	-0.6742384007366056
118	33	-0.7029801201169903
118	34	-2.3637666581956083
118	35	-0.7101192310516566
118	36	-0.7325971845531782
118	37	-0.783452128086445
118	38	-0.7377551454063251
118	39	-0.753524400955655
118	40	-0.7494714340020963
118	41	-0.2617865612960273
118	42	-0.26607772539090035
118	43	-0.2767520596933153
118	44	-0.4044910953197842
118	45	-0.3386834167552877
118	46	-0.39491289473198066
118	47	-0.3882795131136234
118	48	-0.387084357537951
118	49	-0.3462762066260652
118	50	-0.541696453972115
119	1	-0.3261332154344956
119	2	-0.3728446400718326
119	3	-0.3120687204860033
119	4	-0.2937725644157254
119	5	-0.3091630867712212
119	6	-0.257092104199522
119	7	-0.28581259607702236
119	8	-0.3126636448157143
119	9	0.1999820131360579
119	10	-0.22093792186773697
119	11	-0.22767412043407376
119	12	-0.2280284266525996
119	13	-0.1891765020561067
119	14	-0.221748374806225
119	15	-0.264174898820265
119	16	-0.3226555607995891
119	17	-0.4028507359336757
119	18	-0.3889434033602299
119	19	-0.33303168257837257
119	20	-0.3644362298968673
119	21	-0.3682276181730444
119	22	-0.43055156241849346
119	23	-0.3823805091584731
119	24	-0.39737561890419215
119	25	-0.40772111220352514
119	26	-0.3616073063389891
119	27	-0.39186912777847027
119	28	-0.37363467991049504
119	29	-0.4236259150596524
119	30	-0.34891844794550764
119	31	-0.4936070855649035
119	32	-0.5586191299729322
119	33	-1.4473322664565629
119	34	-0.5549339084654932
119	35	-0.4942838288073048
119	36	-0.4662461774318203
119	37	-0.5470048730251236
119	38	-0.5197415661458322
119	39	-0.5563500652275195
119	40	-0.5671161707514756
119	41	-0.3554112820781299
119	42	-0.350764702613958
119	43	-0.3683838586335426
119	44	-0.4297575373426028
119	45	-0.5853016106315039
119	46	-0.5144977127170307
119	47	-0.4286009573372098
119	48	-0.48184226993782175
119	49	-0.43354325373284297
119	50	-0.5002977040056038
120	1	-0.2502279036813988
120	2	-0.2891997151507226
120	3	-0.2898328002495063
120	4	-0.30088005088875036
120	5	-0.3074615658118134
120	6	-0.2175789431460089
120	7	-0.30062426773957895
120	8	-0.26181887683488725
120	9	0.19260866212859185
120	10	-0.1859825943671254
120	11	-0.24255035533982294
120	12	-0.17800377756839936
120	13	-0.2049249228209291
120	14	-0.21062078552493801
120	15	-0.22443984881364543
120	16	-0.32004638405121
120	17	-0.33281690368253436
120	18	-0.3418312450716243
120	19	-0.32985733360943215
120	20	-0.38730891246081
120	21	-0.3664276870363068
120	22	-0.4197636538260312
120	23	-0.47421096992041356
120	24	-0.39977740091682534
120	25	-0.4168962926497102
120	26	-0.36157264921795473
120	27	-0.40538570650970485
120	28	-0.4041736025999967
120	29	-0.3967599130503885
120	30	-0.284836759920843
120	31	-0.2800558706883436
120	32	-0.356056314793193
120	33	-0.28677831309584434
120	34	-0.2826768604425425
120	35	-0.2892921336162928
120	36	-0.25238325035713416
120	37	-0.2722874709342449
120	38	-0.33887436831119544
120	39	-0.33865002221682516
120	40	-0.3926190791913413
120	41	-0.3802015669041836
120	42	-0.37559653246065494
120	43	-0.3837755271014984
120	44	-0.404342889457169
120	45	-0.5052008417352951
120	46	-0.4038578323499067
120	47	-0.39602824791385416
120	48	-0.43119558872030694
120	49	-0.4650305434473907
120	50	-0.42723592014920087
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	20599.0
3	2703.0
4	3564.0
5	3800.0
6	4449.0
7	7909.0
8	8998.0
9	8645.0
10	12191.0
11	17979.0
12	17748.0
13	22689.0
14	29951.0
15	34434.0
16	35673.0
17	39282.0
18	47383.0
19	56164.0
20	61830.0
21	58462.0
22	72934.0
23	89504.0
24	97441.0
25	108151.0
26	135787.0
27	164489.0
28	189463.0
29	214540.0
30	262093.0
31	316433.0
32	383953.0
33	485649.0
34	652184.0
35	927298.0
36	1429303.0
37	2548568.0
38	4836838.0
39	1.6254504E7
40	79895.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	20.256659915298076	28.672912546332935	25.169619891262897	25.900807647106095
2	24.33669131897642	28.216259830751227	26.421933248328617	21.025115601943735
3	23.424619446009682	26.524028123138248	26.607975932876716	23.443376497975354
4	24.54157684305939	26.12495242654861	25.986996141675416	23.346474588716585
5	24.948647569147926	27.331552999178303	26.236176802445442	21.48362262922832
6	24.78624556188389	26.330833020323514	24.972178772918134	23.910742644874457
7	24.059676271909	26.338481576466506	26.61106568565615	22.99077646596834
8	23.604282350283153	26.344378667190256	26.487549540268994	23.563789442257598
9	23.312627693522465	25.994654588502897	26.78116824831941	23.91154946965523
10	23.439076395902564	26.793021529424266	25.94872220735435	23.81917986731882
11	23.664089902089266	26.733449185362673	26.03870684282442	23.563754069723636
12	23.506839145923745	26.375155160055247	26.386606409202955	23.73139928481805
13	23.55732172032775	26.407976844819526	26.31453749049417	23.720163944358546
14	23.53098225224486	26.604623937750393	26.436274437288443	23.428119372716306
15	23.357455146472436	26.379021553631247	26.434257188466177	23.829266111430137
16	23.499416842868992	26.463186462013088	26.464026988290122	23.573369706827798
17	23.81387786499764	26.26837209364876	26.546332170949732	23.371417870403867
18	23.38319524144451	26.23970026372166	26.40426742264187	23.972837072191954
19	23.7422570420312	26.33636187402159	26.44537736978843	23.47600371415878
20	23.73627309103058	26.076933662366397	26.596341167508815	23.590452079094216
21	23.444116155876852	26.381600270042377	26.41561780934847	23.758665764732303
22	23.973062331643774	26.033786228107807	26.27376151008557	23.719389930162844
23	23.61653041271566	26.146335264064597	26.59646080418297	23.640673519036778
24	23.636217350420086	26.169380673363797	26.347667660441143	23.846734315774977
25	23.915356016099274	26.074577229957473	26.244420920234457	23.76564583370879
26	23.877052682493712	26.092178643392682	26.56885434069322	23.461914333420385
27	23.99754821211133	25.83945268647626	26.422450101754848	23.74054899965756
28	24.049396842707786	26.068015167388108	26.25046514538597	23.63212284451813
29	23.88858662910552	26.13118496069205	26.393917257536064	23.58631115266637
30	23.81223398141654	25.96966499556427	26.44460192317897	23.773499099840226
31	23.913216807496976	26.051620312183427	26.352898251608035	23.68226462871156
32	23.834488932261564	26.0298606740636	26.422827642435266	23.71282275123957
33	23.850307314318908	26.032119919244145	26.291985397003657	23.825587369433286
34	23.915283491886612	26.1660094951016	26.2112133736751	23.707493639336686
35	23.864080471560982	26.150950097434677	26.340828325946415	23.644141105057926
36	23.904379763125682	25.95550104962413	26.356194766914825	23.78392442033536
37	23.95158667195357	26.083098128971887	26.157325385886704	23.80798981318784
38	23.874901285880934	26.151923788124375	26.300849547032062	23.67232537896263
39	23.893765641020877	25.977875514737743	26.2856749214508	23.842683922790584
40	23.891428295651252	26.073079316971114	26.22454962597028	23.810942761407354
41	23.866665280043524	26.122260245537976	26.26485356939852	23.74622090501998
42	23.95323033866697	25.954774397037735	26.23838018327834	23.853615081016954
43	23.97336521276508	26.06017988899535	26.11835659801402	23.848098300225548
44	23.884984195502426	26.108632733371778	26.236344654868084	23.77003841625771
45	23.97442026975494	25.877372849512152	26.21387045914321	23.9343364215897
46	23.99786885686309	25.940109706874903	26.049353348339494	24.012668087922513
47	23.97275728932201	26.108260892177903	26.130232666173953	23.78874915232613
48	23.9788320925862	25.902407275534056	26.186486221285826	23.93227441059392
49	23.954723621108446	25.96982001161841	26.135387050025678	23.940069317247467
50	24.011524406121612	26.01702944611384	26.15570564064975	23.8157405071148
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	3815.0
1	2470.0
2	1125.0
3	979.5
4	834.0
5	905.5
6	977.0
7	1256.5
8	1536.0
9	2328.0
10	3120.0
11	5467.0
12	7814.0
13	12293.0
14	16772.0
15	24787.5
16	32803.0
17	49698.5
18	66594.0
19	93602.5
20	120611.0
21	162632.0
22	204653.0
23	266270.5
24	327888.0
25	359324.0
26	390760.0
27	454527.0
28	518294.0
29	584783.0
30	651272.0
31	736453.0
32	821634.0
33	910184.5
34	998735.0
35	1104060.0
36	1209385.0
37	1298153.5
38	1386922.0
39	1471463.0
40	1556004.0
41	1631661.0
42	1707318.0
43	1776571.0
44	1845824.0
45	1958929.5
46	2072035.0
47	2128807.5
48	2185580.0
49	2117302.5
50	2049025.0
51	1988982.0
52	1928939.0
53	1898451.0
54	1867963.0
55	1789767.5
56	1711572.0
57	1620568.5
58	1529565.0
59	1423628.5
60	1317692.0
61	1174668.5
62	1031645.0
63	893272.5
64	754900.0
65	650806.0
66	546712.0
67	453626.0
68	360540.0
69	291313.0
70	222086.0
71	180338.0
72	138590.0
73	107725.5
74	76861.0
75	57123.0
76	37385.0
77	28174.5
78	18964.0
79	13802.0
80	8640.0
81	6346.0
82	4052.0
83	3333.0
84	2614.0
85	2128.0
86	1642.0
87	1203.5
88	765.0
89	583.0
90	401.0
91	283.5
92	166.0
93	132.5
94	99.0
95	100.5
96	102.0
97	95.0
98	88.0
99	127.5
100	167.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0908972319311661
2	0.021204647203353473
3	0.0
4	0.0
5	0.0
6	1.344832548175264E-5
7	0.0
8	0.0
9	0.7730870765626617
10	0.0
11	0.0012338838629508049
12	0.0
13	0.004014325156303163
14	0.0
15	0.0
16	7.060370877920135E-4
17	0.0
18	0.0
19	6.72416274087632E-6
20	5.614675888631727E-4
21	0.0
22	0.0
23	0.0
24	0.0033082880685111494
25	0.00677795604280333
26	0.01829644681792447
27	0.022905860376795185
28	0.01837713677081498
29	0.021406372085579765
30	0.018242653515997458
31	0.020693610835046873
32	0.02143326873654327
33	0.03497909457803861
34	0.038724453224706724
35	0.036108753918505834
36	0.046363102098342227
37	0.05783788581564766
38	0.07157198821388755
39	0.09530828268918096
40	0.10108433848359372
41	0.10425478121591689
42	0.10815143352425473
43	0.12007673614519887
44	0.13418066749418697
45	0.13698128127576195
46	0.13190790048777076
47	0.12501899575974298
48	0.12185863927153111
49	0.13077151698456266
50	0.1166776718796859
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2.974348E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.24502284351573
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.11050377492874	33.23265955309697
2	13.993543148109143	12.382892724996243
3	4.173999393364022	5.540360955246357
4	1.800207829547474	3.1860094616561545
5	0.9541527779438819	2.1108255728165526
6	0.6109521362583912	1.621895473502835
7	0.4337607250924571	1.3434227233235
8	0.31693979944916667	1.121840693331814
9	0.2540535066352461	1.0116542884096567
>10	1.9165612154285954	17.447333154898917
>50	0.29449977097134006	9.105592258506375
>100	0.1396420055318058	10.164301292600678
>500	7.807806277569137E-4	0.20560563449704342
>1k	5.339649638811566E-5	0.0911578740972869
>5k	2.5090015894101657E-4	0.8573568440339571
>10k+	9.883945647292231E-5	0.5770914949855253
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	31407	0.1055928896013513	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	6.72416274087632E-6	6.72416274087632E-6	0.0	3.36208137043816E-6	0.0
2	6.72416274087632E-6	6.72416274087632E-6	0.0	3.36208137043816E-6	0.0
3	6.72416274087632E-6	6.72416274087632E-6	0.0	3.36208137043816E-6	0.0
4	6.72416274087632E-6	6.72416274087632E-6	0.0	3.36208137043816E-6	0.0
5	6.72416274087632E-6	6.72416274087632E-6	0.0	3.36208137043816E-6	0.0
6	1.008624411131448E-5	6.72416274087632E-6	0.0	3.36208137043816E-6	0.0
7	1.008624411131448E-5	1.008624411131448E-5	0.0	3.36208137043816E-6	0.0
8	1.68104068521908E-5	1.008624411131448E-5	0.0	3.36208137043816E-6	0.0
9	3.36208137043816E-5	1.344832548175264E-5	3.36208137043816E-6	3.36208137043816E-6	0.0
10	7.531062269781478E-4	2.017248822262896E-5	3.36208137043816E-6	3.36208137043816E-6	0.0
11	8.00175366164282E-4	2.017248822262896E-5	3.36208137043816E-6	3.36208137043816E-6	0.0
12	8.068995289051584E-4	2.017248822262896E-5	3.36208137043816E-6	6.72416274087632E-6	0.0
13	8.102616102755965E-4	2.017248822262896E-5	3.36208137043816E-6	6.72416274087632E-6	0.0
14	8.136236916460347E-4	2.017248822262896E-5	3.36208137043816E-6	6.72416274087632E-6	0.0
15	8.237099357573492E-4	2.017248822262896E-5	3.36208137043816E-6	1.008624411131448E-5	0.0
16	8.237099357573492E-4	2.017248822262896E-5	3.36208137043816E-6	1.008624411131448E-5	0.0
17	8.438824239799781E-4	2.017248822262896E-5	3.36208137043816E-6	1.008624411131448E-5	0.0
18	8.573307494617307E-4	2.017248822262896E-5	3.36208137043816E-6	1.008624411131448E-5	0.0
19	8.976757259069887E-4	2.017248822262896E-5	3.36208137043816E-6	1.344832548175264E-5	0.0
20	9.380207023522466E-4	2.017248822262896E-5	3.36208137043816E-6	1.344832548175264E-5	0.0
21	9.850898415383808E-4	2.017248822262896E-5	3.36208137043816E-6	1.68104068521908E-5	0.0
22	0.001052331468947144	2.017248822262896E-5	3.36208137043816E-6	2.017248822262896E-5	0.0
23	0.0011094868522445928	2.017248822262896E-5	3.36208137043816E-6	2.3534569593067118E-5	0.0
24	0.0012338838629508046	2.017248822262896E-5	3.36208137043816E-6	2.3534569593067118E-5	3.36208137043816E-6
25	0.001368367117768331	2.017248822262896E-5	3.36208137043816E-6	2.3534569593067118E-5	3.36208137043816E-6
26	0.0015028503725858574	2.017248822262896E-5	3.36208137043816E-6	2.3534569593067118E-5	3.36208137043816E-6
27	0.0016575061156260129	2.017248822262896E-5	3.36208137043816E-6	2.689665096350528E-5	3.36208137043816E-6
28	0.0018155239400366064	2.017248822262896E-5	3.36208137043816E-6	2.689665096350528E-5	3.36208137043816E-6
29	0.002272767006416196	2.017248822262896E-5	3.36208137043816E-6	3.025873233394344E-5	3.36208137043816E-6
30	0.0026795788522392134	2.017248822262896E-5	3.36208137043816E-6	3.025873233394344E-5	3.36208137043816E-6
31	0.003338546800845093	2.017248822262896E-5	3.36208137043816E-6	3.025873233394344E-5	3.36208137043816E-6
32	0.005140622415399946	2.017248822262896E-5	3.36208137043816E-6	3.36208137043816E-5	3.36208137043816E-6
33	0.02230740989285719	2.017248822262896E-5	3.36208137043816E-6	3.36208137043816E-5	3.36208137043816E-6
34	0.023154654398207606	2.017248822262896E-5	3.36208137043816E-6	3.6982895074819756E-5	3.36208137043816E-6
35	0.02344043131469485	2.017248822262896E-5	3.36208137043816E-6	3.6982895074819756E-5	3.36208137043816E-6
36	0.02371612198707078	2.017248822262896E-5	3.36208137043816E-6	3.6982895074819756E-5	3.36208137043816E-6
37	0.024116209670152922	2.689665096350528E-5	3.36208137043816E-6	3.6982895074819756E-5	3.36208137043816E-6
38	0.024469228214048927	2.689665096350528E-5	3.36208137043816E-6	3.6982895074819756E-5	3.36208137043816E-6
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGTATG	2500	0.0	22.226007	13
TATGCCG	2680	0.0	20.978287	16
CGTATGC	2755	0.0	20.486906	14
CTCGTAT	2895	0.0	19.34517	12
GATCGGA	3765	0.0	18.160925	1
ATCGGAA	4450	0.0	15.499294	2
TAGATCG	1520	0.0	14.893271	32
GAGTACT	17150	0.0	12.85929	20
GTACTTT	16895	0.0	12.651739	22
ATGCCGT	4820	0.0	12.165394	17
TCGGAAG	5815	0.0	12.065351	3
AGTACTT	18450	0.0	11.799096	21
GCTCGTA	4950	0.0	11.7134285	11
CCGTCTT	4895	0.0	11.667237	20
AGAGCGG	3615	0.0	11.437652	41
AGCTCGT	5025	0.0	11.2763605	10
GCCGTCT	5085	0.0	11.27343	19
GAGCGGT	3445	0.0	11.172266	42
GAGTACA	18740	0.0	10.8752775	17
AGTACAT	20215	0.0	10.158128	18
>>END_MODULE