FastQCFastQC Report
Thu 26 May 2016
SRR522134_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR522134_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40799856
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG29950.021.80586216
TCGTATG31150.021.03652213
CGTATGC34450.019.21280914
CTCGTAT34950.018.68637312
GATCGGA45350.016.937671
TAGATCG20150.015.61727432
AAGCAGT323050.013.980191
GCTCGTA49000.013.50791411
ATCGGAA60800.013.0935842
GAGCGGT32900.012.44868442
GCCGTCT54000.012.17651519
CCGTCTT57000.012.15399520
AGAGCGG43350.011.93657841
AGCAGTG384050.011.6928812
AGCTCGT58800.011.48097810
ATGCCGT64200.010.54937417
GAGCTCG64150.010.4891419
GAGTACT250900.010.16811920
TCGGAAG79750.010.0364853
TGCCGTC69150.010.01710218
Produced by FastQC (version 0.11.5)