##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR522134_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	40799856
Sequences flagged as poor quality	0
Sequence length	50
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.91935802420479	39.0	36.0	40.0	31.0	40.0
2	36.54256353257718	38.0	35.0	40.0	30.0	40.0
3	36.55599789371806	39.0	35.0	40.0	30.0	40.0
4	36.54162196062653	39.0	35.0	40.0	30.0	40.0
5	36.577358459304364	39.0	36.0	40.0	30.0	40.0
6	36.656216874883086	39.0	36.0	40.0	30.0	40.0
7	36.57527075585757	39.0	35.0	40.0	30.0	40.0
8	36.527184238101235	38.0	35.0	40.0	30.0	40.0
9	36.505035262869555	38.0	35.0	40.0	30.0	40.0
10	36.42347713188008	38.0	35.0	40.0	30.0	40.0
11	36.51430899658077	38.0	35.0	40.0	30.0	40.0
12	36.42159197816777	38.0	35.0	40.0	30.0	40.0
13	36.35573125552208	38.0	35.0	40.0	30.0	40.0
14	36.27849252703245	38.0	35.0	40.0	30.0	40.0
15	36.198340283357865	38.0	35.0	40.0	29.0	40.0
16	36.22727795902025	38.0	35.0	40.0	30.0	40.0
17	36.087462097905444	38.0	35.0	40.0	29.0	40.0
18	35.97117725121383	38.0	35.0	40.0	29.0	40.0
19	35.87374394164529	38.0	35.0	40.0	29.0	40.0
20	35.971270878995256	38.0	35.0	40.0	29.0	40.0
21	36.07505592666798	38.0	35.0	40.0	30.0	40.0
22	35.93904500545296	38.0	35.0	40.0	29.0	40.0
23	35.84139348432994	38.0	35.0	40.0	29.0	40.0
24	35.74031530895599	38.0	35.0	40.0	29.0	40.0
25	35.666417106962335	38.0	35.0	40.0	29.0	40.0
26	35.497355774981166	38.0	35.0	40.0	28.0	40.0
27	35.36808661775669	38.0	35.0	40.0	28.0	40.0
28	35.246820135835776	38.0	35.0	39.0	28.0	40.0
29	35.1409426788173	38.0	34.0	39.0	27.0	40.0
30	34.98018970949309	38.0	34.0	39.0	27.0	40.0
31	35.015287063758265	38.0	35.0	40.0	27.0	40.0
32	34.8278770885858	38.0	34.0	40.0	26.0	40.0
33	34.7088429184652	38.0	34.0	39.0	26.0	40.0
34	34.59443133818904	38.0	33.0	39.0	26.0	40.0
35	34.44485882499193	38.0	33.0	39.0	26.0	40.0
36	34.401446392359816	38.0	34.0	39.0	25.0	40.0
37	34.23354766252116	38.0	33.0	39.0	25.0	40.0
38	34.07229731399052	38.0	33.0	39.0	24.0	40.0
39	33.89531021874195	38.0	33.0	39.0	23.0	40.0
40	33.75718063808853	38.0	33.0	39.0	23.0	40.0
41	33.65364796875754	38.0	33.0	39.0	23.0	40.0
42	33.46302959990839	38.0	33.0	39.0	22.0	40.0
43	33.28377418783047	38.0	33.0	39.0	22.0	40.0
44	33.11775830777442	37.0	33.0	39.0	21.0	40.0
45	32.931735960048485	37.0	33.0	39.0	19.0	40.0
46	32.72588533155607	37.0	33.0	39.0	16.0	40.0
47	32.55664216069782	37.0	32.0	39.0	15.0	40.0
48	32.3941617833161	37.0	32.0	39.0	13.0	40.0
49	32.22501236278873	36.0	32.0	39.0	2.0	40.0
50	32.06063016496921	36.0	32.0	39.0	2.0	40.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1	1	-0.8019214500822542
1	2	-0.6664259028032049
1	3	-0.704020881005178
1	4	-0.6360568212564814
1	5	-0.6783410138756238
1	6	-0.7414329237677109
1	7	-0.7563336400171679
1	8	-0.636143037628166
1	9	-0.699497172516736
1	10	-0.8962289795787868
1	11	-0.7501228085117049
1	12	-0.6166144890084411
1	13	-0.5545945855883332
1	14	-0.6033835771644007
1	15	-0.5474657052735452
1	16	-0.4170211262156016
1	17	-0.405591251608854
1	18	-0.40630771050699366
1	19	-0.4199600129673229
1	20	-0.4219502415196672
1	21	-0.4116734631994916
1	22	-0.46557254103317547
1	23	-0.48437601114807904
1	24	-0.42873223194278864
1	25	-0.4368592867726875
1	26	-0.47447218674545155
1	27	-0.48579799904117493
1	28	-0.5454137891228967
1	29	-0.5049428653154422
1	30	-0.6426049270472873
1	31	-0.6809800218587441
1	32	-0.5920184331851814
1	33	-0.7460362197420594
1	34	-0.8407471580052999
1	35	-0.7339548238332654
1	36	-0.7945643307672299
1	37	-0.7426293002272004
1	38	-0.64407741695711
1	39	-0.608227723647083
1	40	-0.6015987762299773
1	41	-0.42818437184410385
1	42	-0.44391247147292034
1	43	-0.5379581495274266
1	44	-0.47164814580526127
1	45	-0.4525910085073619
1	46	-0.5573543520327107
1	47	-0.6234834134988922
1	48	-0.6507351501787859
1	49	-0.6288509119492893
1	50	-0.8923715932291572
2	1	-0.710397962452511
2	2	-0.7684240625206087
2	3	-0.5955436200255448
2	4	-0.6702261554016928
2	5	-0.7797710355332299
2	6	-0.6595610184859524
2	7	-0.6564858607428476
2	8	-0.7999059523684835
2	9	-0.717365313673433
2	10	-0.7413024178452687
2	11	-0.6113958442730834
2	12	-0.6174886472907701
2	13	-0.6851921851911769
2	14	-0.5849427101015934
2	15	-0.5565343132948328
2	16	-0.37124371283540114
2	17	-0.3745018156435762
2	18	-0.36909284102526385
2	19	-0.3488697117099733
2	20	-0.37341214285407887
2	21	-0.3246101693428969
2	22	-0.313774060321272
2	23	-0.356459585313722
2	24	-0.36704157461085174
2	25	-0.414411598832892
2	26	-0.40016555638536033
2	27	-0.41415786092316154
2	28	-0.4375190006089156
2	29	-0.4620052869793767
2	30	-0.5850937851572269
2	31	-0.5683230091443292
2	32	-0.6416896629745281
2	33	-0.47067933433514497
2	34	-0.6689225085860784
2	35	-0.819898066319368
2	36	-0.5391811277675558
2	37	-0.5068983773714635
2	38	-0.38783712522361213
2	39	-0.4339604433744384
2	40	-0.40227900704339703
2	41	-0.3119153836965296
2	42	-0.3160726379971095
2	43	-0.30587553266171597
2	44	-0.35025846248313286
2	45	-0.33400825487405683
2	46	-0.3329823448627067
2	47	-0.3613311151102181
2	48	-0.3588923451001236
2	49	-0.3872849637392157
2	50	-0.6990090901989099
3	1	-0.5455334043783893
3	2	-0.6951721414559984
3	3	-0.7460297438391805
3	4	-0.7922390532418007
3	5	-0.6706765296008186
3	6	-0.7158583211796739
3	7	-0.7352654591364498
3	8	-0.6625306131249715
3	9	-0.6151907502717577
3	10	-0.766626560013016
3	11	-0.6476602586235316
3	12	-0.542204146138765
3	13	-0.49948531877043223
3	14	-0.5424409122930385
3	15	-0.540967086282059
3	16	-0.39846399903524343
3	17	-0.41330682410293207
3	18	-0.40741680995691354
3	19	-0.3766781420452787
3	20	-0.45056060088864314
3	21	-0.41040086124434083
3	22	-0.4086381171218392
3	23	-0.4467680167012418
3	24	-0.4332129435826175
3	25	-0.45540115407656145
3	26	-0.4972122786160895
3	27	-0.4790644043402281
3	28	-0.5562719725802694
3	29	-0.5349929472322401
3	30	-0.7313300751571532
3	31	-0.5137586566891699
3	32	-0.6294964341827551
3	33	-0.5353222398496698
3	34	-0.6796702639453898
3	35	-0.6139592015747581
3	36	-0.5420517164445329
3	37	-0.4880721820023908
3	38	-0.46268390639502144
3	39	-0.4091766475829317
3	40	-0.45400051132534713
3	41	-0.39780542330261426
3	42	-0.41614358440915566
3	43	-0.3693696420871646
3	44	-0.4014806629502985
3	45	-0.4189024541667905
3	46	-0.432289663515526
3	47	-0.4425876425042077
3	48	-0.5131082703463079
3	49	-0.4661199210838234
3	50	-0.7866116545140756
4	1	-0.559371872523954
4	2	-0.6048003750880255
4	3	-0.6544832897974047
4	4	-0.5779948124767245
4	5	-0.5676331486114137
4	6	-0.6247944817840079
4	7	-0.5645381521124548
4	8	-0.49241764591143067
4	9	-0.5716770947079439
4	10	-0.6427770267331923
4	11	-0.5366806778549673
4	12	-0.5834645610467675
4	13	-0.5459262662075943
4	14	-0.45252588624047263
4	15	-0.47906254799629977
4	16	-0.3477772017092775
4	17	-0.3976465140410852
4	18	-0.35656511378226696
4	19	-0.37329596531304077
4	20	-0.3732824583327954
4	21	-0.3706404165592261
4	22	-0.4058886877982033
4	23	-0.4497154022091223
4	24	-0.4448726995957628
4	25	-0.4179179525918215
4	26	-0.47519064514452936
4	27	-0.4296381641535092
4	28	-0.4657091819645487
4	29	-0.5020403337052031
4	30	-0.6192276758073589
4	31	-0.5820906514266468
4	32	-0.5983539705424761
4	33	-0.5993422802332162
4	34	-0.5607490027588256
4	35	-0.5855435792695047
4	36	-0.48714730523611394
4	37	-0.5199747643226473
4	38	-0.48013214373094115
4	39	-0.457725777352195
4	40	-0.4787358305575182
4	41	-0.40542175381172996
4	42	-0.4489505803219558
4	43	-0.4395865805916159
4	44	-0.43853462937415344
4	45	-0.4906181871593063
4	46	-0.4863743569032408
4	47	-0.5302788845486646
4	48	-0.5656124679237102
4	49	-0.4816333153242489
4	50	-0.567903128253409
5	1	-0.4243313751733808
5	2	-0.6279680216820793
5	3	-0.665862818446783
5	4	-0.4838920463134144
5	5	-0.47834843874392874
5	6	-0.5221755448123062
5	7	-0.6068854885313044
5	8	-0.4454916958787365
5	9	-0.48123624249185326
5	10	-0.5776390774037878
5	11	-0.5914112287933975
5	12	-0.569404270403119
5	13	-0.505238598512527
5	14	-0.6932721583756987
5	15	-0.48798713132354976
5	16	-0.3938080635040109
5	17	-0.3646568957560987
5	18	-0.3662220798989537
5	19	-0.41342469026454864
5	20	-0.402707556333489
5	21	-0.36997325208737664
5	22	-0.4473957102628958
5	23	-0.3674661998823652
5	24	-0.4310747594233746
5	25	-0.4530744234195083
5	26	-0.37749114271333184
5	27	-0.36762077630023526
5	28	-0.4026528992493539
5	29	-0.3984961792867878
5	30	-0.4874213746779432
5	31	-0.3524018740834691
5	32	-0.5036711175469861
5	33	-0.40845232237286666
5	34	-0.3899100820875461
5	35	-0.4654005817533786
5	36	-0.3814620588703974
5	37	-0.45417123338175713
5	38	-0.38579813162346
5	39	-0.34529481693652997
5	40	-0.36981357377086965
5	41	-0.25840230732560343
5	42	-0.33298281085404824
5	43	-0.32855167457847045
5	44	-0.32643886148560597
5	45	-0.25218782871823464
5	46	-0.30928331250169094
5	47	-0.3458569879287907
5	48	-0.3383726336311099
5	49	-0.3211489450138423
5	50	-0.5036498881494005
6	1	-0.5366571036741448
6	2	-0.5988126493742598
6	3	-0.5191710213158984
6	4	-0.5959616114395843
6	5	-0.46211377616639027
6	6	-0.5462230460212609
6	7	-0.4906681219609439
6	8	-0.6036119403817608
6	9	-0.49818336771389937
6	10	-0.6162681100677005
6	11	-0.5308556769112869
6	12	-0.5253176179032977
6	13	-0.49694748948750345
6	14	-0.5336906042894043
6	15	-0.524571144286206
6	16	-0.39163516007631216
6	17	-0.3736943141755944
6	18	-0.4266739397827237
6	19	-0.3854700664978665
6	20	-0.41906764610018143
6	21	-0.40503352337152165
6	22	-0.49839083822848806
6	23	-0.42157676291473223
6	24	-0.4764572404059422
6	25	-0.45038352376517565
6	26	-0.45644628242372676
6	27	-0.46386339518284814
6	28	-0.508786410106822
6	29	-0.49095689319521085
6	30	-0.6225922569708544
6	31	-0.49880082381960733
6	32	-0.4710720080387176
6	33	-0.5281308082207943
6	34	-0.5420020600280822
6	35	-0.6322888639965925
6	36	-0.4712104396847536
6	37	-0.4629371193734997
6	38	-0.4992620337952758
6	39	-0.46652383044026635
6	40	-0.4594266678315293
6	41	-0.41321736495140726
6	42	-0.4242893420829503
6	43	-0.5241626292239943
6	44	-0.662932897947691
6	45	-0.5317335459150812
6	46	-0.5769519199914512
6	47	-0.606064561076824
6	48	-0.5986451338900025
6	49	-0.5791210297382108
6	50	-0.5933389809527121
7	1	-0.5833102085514952
7	2	-0.5579064175370263
7	3	-0.5595257626761239
7	4	-0.6151556606272024
7	5	-0.6329775021087585
7	6	-0.5446681527188275
7	7	-0.5303598052528997
7	8	-0.5089146949684036
7	9	-0.4888994424360149
7	10	-0.6442897695326337
7	11	-0.39618393758891557
7	12	-0.4943404154820925
7	13	-0.4395540100066455
7	14	-0.4527065853977632
7	15	-0.3748623901446564
7	16	-0.395959915944168
7	17	-0.42334006554130355
7	18	-0.4174469721425993
7	19	-0.40056160097502413
7	20	-0.45792965023941434
7	21	-0.4511764365740163
7	22	-0.42469804094547925
7	23	-0.4812701950230789
7	24	-0.42211316659943776
7	25	-0.4332314657242975
7	26	-0.3856139796296816
7	27	-0.4507538330963783
7	28	-0.48242158662554857
7	29	-0.4710155034796273
7	30	-0.5547358364532897
7	31	-0.47167598767364893
7	32	-0.5147204769650315
7	33	-0.4794643745834506
7	34	-0.6203561040030436
7	35	-0.6685795654800515
7	36	-0.4637472516936896
7	37	-0.4490342533484579
7	38	-0.4972947885092154
7	39	-0.5437674723578994
7	40	-0.4656613174209596
7	41	-0.44031673279157957
7	42	-0.4952282273602222
7	43	-0.45739885330085883
7	44	-0.5237890662221645
7	45	-0.564773816051499
7	46	-0.540269038152843
7	47	-0.4775193042234136
7	48	-0.5629833905100199
7	49	-0.5547640478259375
7	50	-0.6235629815734214
8	1	-0.6238977431268111
8	2	-0.536472960178763
8	3	-0.486764427155407
8	4	-0.5254324205000884
8	5	-0.5688193023642043
8	6	-0.6923188676506342
8	7	-0.5814499102744364
8	8	-0.516772088249219
8	9	-0.5585436426194832
8	10	-0.6619762092388015
8	11	-0.6063907596692744
8	12	-0.586547916733231
8	13	-0.6154337808030661
8	14	-0.4700379332813185
8	15	-0.5038273190833351
8	16	-0.41402381674210176
8	17	-0.47901974069729647
8	18	-0.3909565216283468
8	19	-0.4111343992387049
8	20	-0.43716972281486477
8	21	-0.37649117046167646
8	22	-0.40889578570274665
8	23	-0.377201677294309
8	24	-0.3617973359928044
8	25	-0.39444860266106474
8	26	-0.38456004400855903
8	27	-0.42827253133037857
8	28	-0.43252423697359177
8	29	-0.4849213159506789
8	30	-0.5363376048087147
8	31	-0.5088431201693382
8	32	-0.47016966744558175
8	33	-0.6291867958409938
8	34	-0.46271038097878403
8	35	-0.468767948449333
8	36	-0.45585722543437157
8	37	-0.4401996800422552
8	38	-0.4223808441933983
8	39	-0.41121501779890934
8	40	-0.48196728674237477
8	41	-0.4458993264132687
8	42	-0.49539019490212866
8	43	-0.40541242736245664
8	44	-0.401389808991091
8	45	-0.3855085309179529
8	46	-0.317740175254535
8	47	-0.30431369731795854
8	48	-0.4209608532747353
8	49	-0.4227325462383327
8	50	-0.46731156991303635
9	1	-0.4809279446441792
9	2	-0.6317999344647092
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114	34	-0.1804723608026748
114	35	-0.14085524299181174
114	36	-0.08129154506679726
114	37	-0.11964419326693587
114	38	-0.07862524497260637
114	39	-0.18184307331205218
114	40	-0.09818935234427073
114	41	-0.06171433075864741
114	42	-0.10273101145130425
114	43	-0.1621305590884532
114	44	-0.07855027748856713
114	45	-0.10294135237755597
114	46	-0.07195163821646844
114	47	-0.11307686684096296
114	48	-0.08464440802932671
114	49	-0.08637707615830692
114	50	-0.0713257369970961
115	1	-0.6940687571223378
115	2	-1.0341572455985855
115	3	-1.050769415935136
115	4	-1.0011272350673082
115	5	-0.9264343609215828
115	6	-0.8777143216534142
115	7	-0.8526608777930065
115	8	-0.7876600773938733
115	9	-0.7667456274559044
115	10	-0.7141184656675392
115	11	-0.5443138701606998
115	12	-0.5752606891293723
115	13	-0.5444089020321741
115	14	-0.5761490836369916
115	15	-0.5950273252203715
115	16	-0.5039951031850194
115	17	-0.5658034804785856
115	18	-0.5017620368244735
115	19	-0.49549796884915054
115	20	-0.529615079880287
115	21	-0.5191256395711292
115	22	-0.508642103062769
115	23	-0.5453317649823433
115	24	-0.46132274734597445
115	25	-0.5275564152844652
115	26	-0.5087450136785208
115	27	-0.5332355271714562
115	28	-0.5025917413348964
115	29	-0.5297535201478638
115	30	-0.593572858479682
115	31	-0.5207184286079709
115	32	-0.6358503223989587
115	33	-0.472287666258687
115	34	-0.45536050960280505
115	35	-0.5308003569836757
115	36	-0.3983763242290266
115	37	-0.4805955489410465
115	38	-0.39136848681577874
115	39	-0.5201301825191891
115	40	-0.41578657320374646
115	41	-0.3911351084823167
115	42	-0.3058669526393132
115	43	-0.36078549131433846
115	44	-0.31488689947985193
115	45	-0.35640494450139926
115	46	-0.2346462786526473
115	47	-0.2732095855870966
115	48	-0.2525583555511943
115	49	-0.2912803490317586
115	50	-0.3154764096650169
116	1	-0.46192276197836435
116	2	-0.5890113373240311
116	3	-0.6268208012832588
116	4	-0.5681615478202175
116	5	-0.40788502546269
116	6	-0.5263486174547012
116	7	-0.5351736719372227
116	8	-0.5326115831958589
116	9	-0.577945343081474
116	10	-0.5679801161446179
116	11	-0.367883639579091
116	12	-0.49011960771447605
116	13	-0.43929187812220505
116	14	-0.4679614255471307
116	15	-0.4076116305331752
116	16	-0.3426590445422306
116	17	-0.3889532496404584
116	18	-0.3454029254621389
116	19	-0.33690105630798683
116	20	-0.4249803935017695
116	21	-0.3759503328062195
116	22	-0.41972027325648753
116	23	-0.47609244669950357
116	24	-0.5062822516858105
116	25	-0.4038468899142984
116	26	-0.4030248931449023
116	27	-0.3416421690912159
116	28	-0.4680957503191365
116	29	-0.34746772854909125
116	30	-0.324826852230494
116	31	-0.24653707715500417
116	32	-0.2702785070978351
116	33	-0.23345816009934595
116	34	-0.21932848376161473
116	35	-0.26479417598788757
116	36	-0.09136095081448303
116	37	-0.18549140815648002
116	38	-0.13004960175610591
116	39	-0.07748569753761103
116	40	-0.1336428078922367
116	41	-0.187773851278898
116	42	-0.0649541483940439
116	43	-0.09110560805907397
116	44	-0.01939245607283624
116	45	-0.03030857149956745
116	46	-0.1002991261117927
116	47	-0.04092811737996982
116	48	-0.09243301094429057
116	49	-0.09927822229060013
116	50	-0.12179845622052099
117	1	-0.36733611012670764
117	2	-0.4544476732443101
117	3	-0.6599757512207916
117	4	-0.5791870643998678
117	5	-0.47808264113112386
117	6	-0.4163154182988791
117	7	-0.4635281990326803
117	8	-0.40560317636645493
117	9	-0.4668497914454761
117	10	-0.4618797372918735
117	11	-0.5193989372364811
117	12	-0.435592353717027
117	13	-0.4199315667373398
117	14	-0.4059126583117205
117	15	-0.43243709231126815
117	16	-0.37974095787690487
117	17	-0.3950547699861602
117	18	-0.3940701669184392
117	19	-0.4149364571458136
117	20	-0.43008931278233575
117	21	-0.4172431309216975
117	22	-0.4686022735389912
117	23	-0.40259964845616025
117	24	-0.5049125600949367
117	25	-0.4472452229549404
117	26	-0.30334090815629366
117	27	-0.3512741527714027
117	28	-0.3703761911805401
117	29	-0.34893302982444396
117	30	-0.34733774652585936
117	31	-0.27056494274980736
117	32	-0.2802884581572016
117	33	-0.2883947779755047
117	34	-0.16417800766493684
117	35	-0.2947609501503905
117	36	-0.14245204775788523
117	37	-0.20891457641810263
117	38	-0.2458039172947295
117	39	-0.18069453340487485
117	40	-0.1530238925883296
117	41	-0.08863544628319175
117	42	-0.14245071982286106
117	43	-0.11082176755176221
117	44	-0.1803925833907698
117	45	-0.12742356196293514
117	46	-0.04061857078795583
117	47	-0.10912372126158232
117	48	-0.10450971348335969
117	49	0.002705137655702572
117	50	-0.04622296676662074
118	1	-0.5331360332032133
118	2	-0.6077483474321497
118	3	-0.5300225623317516
118	4	-0.46958836926170733
118	5	-0.5382250099412005
118	6	-0.4834369862086447
118	7	-0.44243011580939395
118	8	-0.4409630297710976
118	9	-0.5018546751250597
118	10	-0.4224523077323141
118	11	-0.3806152117513122
118	12	-0.42458171427530544
118	13	-0.42422507624350203
118	14	-0.38917211519915185
118	15	-0.39222807703196594
118	16	-0.44580254910791695
118	17	-0.43606258977528256
118	18	-0.39806099038011666
118	19	-0.43539571563588453
118	20	-0.47318172594852825
118	21	-0.4800704818911825
118	22	-0.5954640163750895
118	23	-0.5566352665679659
118	24	-0.6239568276245748
118	25	-0.5239512196754035
118	26	-0.5444133162894715
118	27	-0.4705365269295214
118	28	-0.47326543653805686
118	29	-0.43186078453400256
118	30	-0.48795283328772854
118	31	-0.31235579244048495
118	32	-0.4351425775425497
118	33	-0.3718037383610451
118	34	-0.3220009270873234
118	35	-0.38051614509902265
118	36	-0.29992307928836226
118	37	-0.33373429699538093
118	38	-0.3530413146041198
118	39	-0.5410536733172577
118	40	-0.517274373528231
118	41	-0.4729314188326228
118	42	-0.38256697016532826
118	43	-0.4555734568868459
118	44	-0.41131397833797223
118	45	-0.5638231477051505
118	46	-0.25751549279839736
118	47	-0.4199108810931591
118	48	-0.3851589292121247
118	49	-0.40944800358589717
118	50	-0.381110884618149
119	1	-0.4610206799560572
119	2	-0.4258275680561283
119	3	-0.50252396284138
119	4	-0.5201504372553742
119	5	-0.46431772098634383
119	6	-0.44447667940194435
119	7	-0.4914915131599287
119	8	-0.5068709889562157
119	9	-0.5054730691130374
119	10	-0.44483377418863057
119	11	-0.45914983480763993
119	12	-0.46032536148325676
119	13	-0.49022548459067394
119	14	-0.46444266778628673
119	15	-0.4948440746881957
119	16	-0.5178493970491402
119	17	-0.5083590043205959
119	18	-0.4680571209444153
119	19	-0.49337299088983855
119	20	-0.4757427164114887
119	21	-0.4770331749074046
119	22	-0.6500953100082256
119	23	-0.5656189160999574
119	24	-0.5905610022320573
119	25	-0.5602611243007232
119	26	-0.6359667203269694
119	27	-0.5401087160381834
119	28	-0.5149999082703758
119	29	-0.5258279773824412
119	30	-0.45178557221059634
119	31	-0.43889440350717734
119	32	-0.49836494710889667
119	33	-0.4079144423151462
119	34	-0.38569413918544626
119	35	-0.4311541898896891
119	36	-0.3805867913888221
119	37	-0.3748353008431735
119	38	-0.4044619324218601
119	39	-0.45555964369282975
119	40	-0.3980618817130903
119	41	-0.5945232592673264
119	42	-0.39832966569213824
119	43	-0.4816794795723496
119	44	-0.41632272871493825
119	45	-0.5340430171045369
119	46	-0.30711464366794416
119	47	-0.4245522355072282
119	48	-0.42708842634214506
119	49	-0.4051254130840043
119	50	-0.3765261687391366
120	1	-0.42567424464176895
120	2	-0.4747575639449906
120	3	-0.4834104568040303
120	4	-0.5098277234758584
120	5	-0.46272037725825754
120	6	-0.4705126557203556
120	7	-0.47194924756220047
120	8	-0.4978164693469509
120	9	-0.5047425329575503
120	10	-0.46077675109685146
120	11	-0.4932943770841618
120	12	-0.5588489764348878
120	13	-0.5108871018707788
120	14	-0.5648926012098201
120	15	-0.5533391238627487
120	16	-0.7393309989364809
120	17	-0.5596342488375825
120	18	-0.5460960051581623
120	19	-0.5848098647636633
120	20	-0.5717075852800377
120	21	-0.5945454466334255
120	22	-0.6208000417683905
120	23	-0.6228922104499546
120	24	-0.6292652896149704
120	25	-0.5670724536519245
120	26	-0.5805778764899188
120	27	-0.5242480085524193
120	28	-0.5909040859827357
120	29	-0.5473822329496869
120	30	-0.4914633746180499
120	31	-0.4401921295933491
120	32	-0.4292647847231734
120	33	-0.3952199033197843
120	34	-0.37506947691035464
120	35	-0.327828391914899
120	36	-0.27501471089011176
120	37	-0.29094089267086076
120	38	-0.4794650273584935
120	39	-0.4428922834223812
120	40	-0.4082633648799501
120	41	-0.3622540713473299
120	42	-0.3466061785917063
120	43	-0.35500438317917116
120	44	-0.3288865476096632
120	45	-0.3824429720141751
120	46	-0.3111302325337917
120	47	-0.3831436285756169
120	48	-0.40301143929449523
120	49	-0.4269850901187837
120	50	-0.3585047803014696
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	81410.0
3	8268.0
4	14154.0
5	14010.0
6	20817.0
7	37637.0
8	41341.0
9	44487.0
10	62500.0
11	78469.0
12	85261.0
13	104652.0
14	124647.0
15	137670.0
16	145044.0
17	160180.0
18	178593.0
19	195291.0
20	204439.0
21	223538.0
22	262494.0
23	306185.0
24	354068.0
25	420282.0
26	506420.0
27	594541.0
28	686824.0
29	795408.0
30	924554.0
31	1073613.0
32	1262582.0
33	1538255.0
34	1956291.0
35	2597306.0
36	3589271.0
37	5146219.0
38	6254238.0
39	1.0567236E7
40	1661.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	19.861063343958307	30.791205321005037	25.68813460490264	23.659596730134012
2	24.24118579636352	30.584904355233377	26.445160148857934	18.728749699545173
3	23.609362738927313	29.233845825338207	26.557652556420784	20.599138879313692
4	24.196690792242013	28.91174910029094	26.375365148347583	20.516194959119463
5	25.04429427397979	29.708739658296835	26.29645310512861	18.95051296259477
6	23.924830605994877	28.105225877055922	25.69087386350685	22.279069653442356
7	23.094755530509715	28.089405511627298	27.31057678242786	21.50526217543513
8	22.577719391950797	28.08032459722407	27.256385414693618	22.085570596131515
9	22.353485757400712	27.5961709276621	27.72497775482345	22.32536556011374
10	22.512440889933252	28.35299122293297	26.63346530641597	22.501102580717806
11	22.718475547484374	28.353919530240248	26.714328561187962	22.213276361087416
12	22.61736413971657	28.11588109526661	26.929425927385626	22.337328837631194
13	22.55961180359962	28.17817812420204	26.81716814845574	22.445041923742597
14	22.63115571302566	28.322692320164077	27.0480501603891	21.998101806421168
15	22.52430253675405	28.03530728147668	26.963825558600014	22.47656462316926
16	22.603829147823944	28.080298093308524	27.042350981409758	22.273521777457773
17	23.010458664363913	27.836593344839255	27.113634910868313	22.03931307992852
18	22.59421454820821	27.89383864492071	26.970636857149692	22.54130994972139
19	22.944148059026276	27.908869882661964	27.008420252213444	22.138561806098313
20	22.978446296477127	27.59515621819842	27.231946602948792	22.194450882375662
21	22.71520962529076	27.909282897328442	27.013554070103396	22.361953407277397
22	23.043493094681512	27.55442568228672	26.90197485010731	22.500106372924453
23	22.798109875681913	27.7752499910784	27.191762637593627	22.234877495646064
24	22.842392770457458	27.65648711752301	26.980483496026014	22.52063661599352
25	23.1062242353021	27.513148170335107	26.83003500753663	22.550592586826163
26	23.162205245109043	27.630606743215747	27.06465303473341	22.142534976941803
27	23.18939924216073	27.368997868137317	26.998447515246056	22.443155374455895
28	23.264665854913062	27.51823645013266	26.841993548738024	22.375104146216252
29	23.231002463520937	27.559140805090117	26.952880289650636	22.256976441738306
30	23.10893397512573	27.396350800598867	26.981974139676186	22.512741084599213
31	23.11171765689714	27.481990145050126	26.9636146236948	22.44267757435793
32	23.109206563094	27.53424645108377	27.00800520286335	22.34854178295888
33	23.065582936758325	27.453122339369806	26.911209165666826	22.57008555820504
34	23.13574673492324	27.56645462332162	26.839899326100213	22.457899315654927
35	23.183855451144144	27.59960445132504	26.886134460560097	22.330405636970717
36	23.09327988162574	27.42193343595921	26.91822194276395	22.5665647396511
37	23.15209165198105	27.512988984438724	26.789517388048022	22.5454019755322
38	23.17374226846681	27.545282254754238	26.87472609994106	22.40624937683789
39	23.099631894297364	27.380871981185745	26.873087615035026	22.646408509481862
40	23.112882271103473	27.51503334140319	26.79977386078346	22.572310526709874
41	23.169537372785324	27.558604550986075	26.857239757609623	22.414618318618977
42	23.174998036032292	27.380143626516297	26.8038264420761	22.641031895375306
43	23.199185655551428	27.5011127327669	26.72604856675186	22.573653044929817
44	23.188569831295826	27.49734066408977	26.83737625605997	22.476713248554436
45	23.1138013500999	27.372799672353214	26.81102375925108	22.7023752182958
46	23.24822179591361	27.447008234784438	26.66662691189179	22.638143057410165
47	23.249417809983978	27.47924851477131	26.726315479506173	22.545018195738542
48	23.166283072497887	27.328414150668035	26.75824586367948	22.747056913154598
49	23.23650753730861	27.422846223119567	26.65997386095181	22.68067237862001
50	23.23693090987215	27.464622143546286	26.70854536017817	22.589901586403396
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	7478.0
1	4685.0
2	1892.0
3	1804.0
4	1716.0
5	1818.0
6	1920.0
7	2546.0
8	3172.0
9	4770.0
10	6368.0
11	10764.5
12	15161.0
13	24235.5
14	33310.0
15	50365.0
16	67420.0
17	100573.5
18	133727.0
19	185428.0
20	237129.0
21	312484.0
22	387839.0
23	496203.5
24	604568.0
25	673017.0
26	741466.0
27	854239.5
28	967013.0
29	1081139.0
30	1195265.0
31	1335184.0
32	1475103.0
33	1609802.0
34	1744501.0
35	1902819.0
36	2061137.0
37	2181439.5
38	2301742.0
39	2397162.0
40	2492582.0
41	2575677.0
42	2658772.0
43	2696905.5
44	2735039.0
45	2741787.5
46	2748536.0
47	2683778.0
48	2619020.0
49	2589862.5
50	2560705.0
51	2511829.0
52	2462953.0
53	2379696.0
54	2296439.0
55	2154258.5
56	2012078.0
57	1865658.5
58	1719239.0
59	1562776.5
60	1406314.0
61	1228268.0
62	1050222.0
63	893463.5
64	736705.0
65	624637.0
66	512569.0
67	420978.5
68	329388.0
69	265323.0
70	201258.0
71	162271.5
72	123285.0
73	97000.0
74	70715.0
75	53638.0
76	36561.0
77	27869.0
78	19177.0
79	14377.5
80	9578.0
81	7220.0
82	4862.0
83	3668.5
84	2475.0
85	1971.0
86	1467.0
87	1217.0
88	967.0
89	732.0
90	497.0
91	363.5
92	230.0
93	173.5
94	117.0
95	92.5
96	68.0
97	58.5
98	49.0
99	55.5
100	62.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.07860076761055235
2	0.00826963703009148
3	0.0
4	0.0
5	0.0
6	1.470593425623855E-5
7	0.0
8	0.0
9	0.0
10	2.9902066321018387E-4
11	2.622558275695875E-4
12	0.0
13	7.401986908973404E-4
14	3.186285755518353E-5
15	0.0
16	6.911789100432119E-4
17	0.0
18	0.0
19	3.1372659746642243E-4
20	0.0
21	1.9607912341651402E-5
22	0.0
23	0.0
24	0.0030833442157246834
25	0.004372564452188262
26	0.01658584285199438
27	0.019188793215348603
28	0.012370141698539328
29	0.02119125126323975
30	0.01720104110171369
31	0.021024584008335713
32	0.02453194932844861
33	0.03473296572419275
34	0.03896827478998945
35	0.03425992483895041
36	0.047473206768180745
37	0.05636294402607695
38	0.07344878864278345
39	0.08895374532694429
40	0.09589249530684618
41	0.09639004608251558
42	0.09933858590089142
43	0.11417932455447882
44	0.12720878230550617
45	0.1312210513684166
46	0.12263278576277328
47	0.11901022395765318
48	0.11556658435265066
49	0.12652985834067648
50	0.10835087261092294
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	4.0799856E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.69972125433427
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.14315622612398	29.24950062528591
2	15.478471211250802	12.908958699047925
3	5.891040509429173	7.369642414235638
4	2.740878123880283	4.571754150316419
5	1.4354949856631924	2.9929870382074846
6	0.8231866189844332	2.0595991531169244
7	0.5482152034495708	1.6002294819864677
8	0.3900661817364529	1.3012520839322064
9	0.2748106649141946	1.0313575312176158
>10	1.835263962980679	15.354091153295526
>50	0.25916439341751435	7.610433275635437
>100	0.1770652515052112	12.93195237411088
>500	0.003021618123432001	0.7712920918217845
>1k	1.3570889833725247E-4	0.0773423729938192
>5k	2.347171435855916E-5	0.07708500113381435
>10k+	5.867928452400633E-6	0.09252255366216941
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.4509890427064252E-6	0.0	0.0	0.0	2.4509890427064252E-6
2	4.9019780854128505E-6	2.4509890427064252E-6	0.0	0.0	4.9019780854128505E-6
3	4.9019780854128505E-6	9.803956170825701E-6	0.0	2.4509890427064252E-6	7.352967128119276E-6
4	4.9019780854128505E-6	1.2254945213532127E-5	0.0	4.9019780854128505E-6	7.352967128119276E-6
5	4.9019780854128505E-6	1.7156923298944976E-5	0.0	7.352967128119276E-6	7.352967128119276E-6
6	7.352967128119276E-6	1.7156923298944976E-5	0.0	9.803956170825701E-6	7.352967128119276E-6
7	7.352967128119276E-6	2.2058901384357828E-5	0.0	9.803956170825701E-6	7.352967128119276E-6
8	7.352967128119276E-6	2.9411868512477103E-5	0.0	1.4705934256238551E-5	7.352967128119276E-6
9	1.2254945213532127E-5	3.431384659788995E-5	0.0	2.2058901384357828E-5	7.352967128119276E-6
10	2.9902066321018387E-4	3.676483564059638E-5	0.0	2.4509890427064254E-5	7.352967128119276E-6
11	3.1617758650912887E-4	4.1666813726009227E-5	0.0	2.6960879469770677E-5	7.352967128119276E-6
12	3.1617758650912887E-4	4.1666813726009227E-5	0.0	3.431384659788995E-5	7.352967128119276E-6
13	3.1617758650912887E-4	4.1666813726009227E-5	0.0	3.676483564059638E-5	7.352967128119276E-6
14	3.1617758650912887E-4	4.1666813726009227E-5	0.0	3.676483564059638E-5	7.352967128119276E-6
15	3.235305536372481E-4	4.4117802768715656E-5	0.0	3.676483564059638E-5	7.352967128119276E-6
16	3.2598154267995456E-4	4.4117802768715656E-5	0.0	3.9215824683302804E-5	7.352967128119276E-6
17	3.406874769361931E-4	4.901978085412851E-5	0.0	4.1666813726009227E-5	7.352967128119276E-6
18	3.4558945502160594E-4	5.3921758939541354E-5	0.0	4.901978085412851E-5	7.352967128119276E-6
19	3.774523125767895E-4	5.3921758939541354E-5	0.0	5.3921758939541354E-5	7.352967128119276E-6
20	3.8480527970490876E-4	5.637274798224778E-5	0.0	5.8823737024954206E-5	7.352967128119276E-6
21	4.09315170131973E-4	5.637274798224778E-5	0.0	6.127472606766064E-5	7.352967128119276E-6
22	4.26472093430918E-4	5.637274798224778E-5	0.0	6.617670415307348E-5	7.352967128119276E-6
23	4.460800057725694E-4	6.127472606766064E-5	0.0	7.107868223848633E-5	7.352967128119276E-6
24	4.926487975839915E-4	6.127472606766064E-5	0.0	8.088263840931203E-5	7.352967128119276E-6
25	5.294136332245878E-4	6.372571511036706E-5	0.0	8.333362745201845E-5	7.352967128119276E-6
26	5.882373702495421E-4	6.372571511036706E-5	0.0	8.578461649472489E-5	7.352967128119276E-6
27	6.421591291890834E-4	6.617670415307348E-5	0.0	8.823560553743131E-5	9.803956170825701E-6
28	7.573556141962854E-4	6.86276931957799E-5	0.0	9.313758362284416E-5	9.803956170825701E-6
29	9.85297595167983E-4	7.107868223848633E-5	0.0	9.803956170825702E-5	9.803956170825701E-6
30	0.0011911806747553226	7.352967128119276E-5	0.0	1.0539252883637628E-4	9.803956170825701E-6
31	0.0015710839763748185	7.598066032389918E-5	0.0	1.0784351787908271E-4	9.803956170825701E-6
32	0.0031960897116891786	7.598066032389918E-5	0.0	1.1029450692178913E-4	9.803956170825701E-6
33	0.018267221335290986	7.843164936660561E-5	0.0	1.1519648500720199E-4	9.803956170825701E-6
34	0.018892223541181127	8.088263840931203E-5	0.0	1.1764747404990841E-4	9.803956170825701E-6
35	0.019056439807042455	8.088263840931203E-5	0.0	1.2009846309261483E-4	9.803956170825701E-6
36	0.01924516596333085	8.333362745201845E-5	0.0	1.2254945213532127E-4	9.803956170825701E-6
37	0.01943879409770466	8.333362745201845E-5	0.0	1.2745143022073412E-4	9.803956170825701E-6
38	0.019654481133462826	8.578461649472489E-5	0.0	1.2745143022073412E-4	9.803956170825701E-6
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATGCCG	2995	0.0	21.805862	16
TCGTATG	3115	0.0	21.036522	13
CGTATGC	3445	0.0	19.212809	14
CTCGTAT	3495	0.0	18.686373	12
GATCGGA	4535	0.0	16.93767	1
TAGATCG	2015	0.0	15.617274	32
AAGCAGT	32305	0.0	13.98019	1
GCTCGTA	4900	0.0	13.507914	11
ATCGGAA	6080	0.0	13.093584	2
GAGCGGT	3290	0.0	12.448684	42
GCCGTCT	5400	0.0	12.176515	19
CCGTCTT	5700	0.0	12.153995	20
AGAGCGG	4335	0.0	11.936578	41
AGCAGTG	38405	0.0	11.692881	2
AGCTCGT	5880	0.0	11.480978	10
ATGCCGT	6420	0.0	10.549374	17
GAGCTCG	6415	0.0	10.489141	9
GAGTACT	25090	0.0	10.168119	20
TCGGAAG	7975	0.0	10.036485	3
TGCCGTC	6915	0.0	10.017102	18
>>END_MODULE