FastQCFastQC Report
Thu 26 May 2016
SRR522133_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR522133_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30847454
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG19850.017.65519716
TCGTATG20200.016.59095813
CGTATGC23000.015.23720414
CTCGTAT22300.015.12680612
GATCGGA27000.013.1942831
GCTCGTA27250.012.78091711
GAGTACT201950.011.08629220
GTACTTT209400.010.59898222
AAGCAGT350600.010.4133011
GAGTACA263300.010.3902217
AGTACTT215200.010.33336521
TAGATCG12800.010.27555132
GCCGTCT33600.010.23517619
GTACATG268100.010.09032319
ATCGGAA39550.09.8209322
TACATGG277250.09.7504120
AGTACAT286750.09.49484418
AGCAGTG389700.09.4833162
AGCTCGT39000.09.32344310
CCGTCTT38300.09.32326620
Produced by FastQC (version 0.11.5)