##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR522133_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 30847454 Sequences flagged as poor quality 0 Sequence length 50 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.14857673505243 36.0 30.0 39.0 19.0 40.0 2 31.873417624676577 36.0 29.0 39.0 13.0 40.0 3 31.76813454361582 36.0 29.0 39.0 12.0 40.0 4 31.886199976179558 36.0 29.0 39.0 13.0 40.0 5 32.46667407300453 37.0 30.0 39.0 17.0 40.0 6 33.060042524092914 37.0 31.0 39.0 19.0 40.0 7 32.96247294833473 37.0 31.0 39.0 19.0 40.0 8 32.91276271941276 37.0 31.0 39.0 19.0 40.0 9 32.8771490509395 37.0 31.0 39.0 19.0 40.0 10 32.804438350082314 37.0 30.0 39.0 19.0 40.0 11 34.07510399399575 38.0 33.0 39.0 25.0 40.0 12 33.94244302949605 38.0 32.0 39.0 25.0 40.0 13 33.864563150009076 37.0 32.0 39.0 25.0 40.0 14 33.78578741052665 37.0 31.0 39.0 25.0 40.0 15 33.69430961790234 37.0 31.0 39.0 24.0 40.0 16 33.76464209979858 38.0 32.0 39.0 24.0 40.0 17 33.621529575828205 37.0 31.0 39.0 24.0 40.0 18 33.47831558481293 37.0 31.0 39.0 23.0 40.0 19 33.383047528006685 36.0 31.0 39.0 23.0 40.0 20 33.46654154991203 37.0 31.0 39.0 23.0 40.0 21 33.61448753598919 38.0 32.0 39.0 23.0 40.0 22 33.439601465975116 37.0 31.0 39.0 23.0 40.0 23 33.332389895127164 37.0 31.0 39.0 23.0 40.0 24 33.21145048145627 36.0 31.0 39.0 23.0 40.0 25 33.12099812191956 36.0 31.0 39.0 23.0 40.0 26 32.92697351943535 37.0 31.0 39.0 21.0 40.0 27 32.771728648983476 36.0 31.0 39.0 21.0 40.0 28 32.6384798239751 36.0 31.0 39.0 21.0 40.0 29 32.51184759040406 36.0 31.0 39.0 20.0 40.0 30 32.353847743804074 36.0 31.0 39.0 19.0 40.0 31 32.36227174534404 36.0 31.0 39.0 17.0 40.0 32 32.16430607206676 36.0 31.0 39.0 17.0 40.0 33 32.0307212711947 36.0 31.0 39.0 17.0 40.0 34 31.89153043230083 36.0 30.0 39.0 17.0 40.0 35 31.715663859973663 36.0 30.0 39.0 16.0 40.0 36 31.6487997356281 36.0 31.0 39.0 10.0 40.0 37 31.491529835817243 36.0 30.0 39.0 9.0 40.0 38 31.32527122011431 36.0 30.0 39.0 5.0 40.0 39 31.130743464274232 35.0 30.0 39.0 2.0 40.0 40 30.96746953573543 35.0 30.0 39.0 2.0 40.0 41 30.853261666262636 36.0 30.0 39.0 2.0 40.0 42 30.63661205232691 35.0 29.0 39.0 2.0 40.0 43 30.4496488105631 35.0 29.0 38.0 2.0 40.0 44 30.245488071722225 35.0 29.0 38.0 2.0 40.0 45 30.038064502827364 35.0 29.0 38.0 2.0 40.0 46 29.807299558660496 35.0 29.0 38.0 2.0 40.0 47 29.626660858299683 35.0 28.0 38.0 2.0 40.0 48 29.426156628679955 35.0 28.0 38.0 2.0 39.0 49 29.235432687572853 35.0 27.0 38.0 2.0 39.0 50 29.054723932808198 35.0 27.0 38.0 2.0 39.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 2.0292892757719514 1 2 -0.43292315191429864 1 3 -5.152383493190882 1 4 1.4349350091131434 1 5 0.6939339578569879 1 6 0.939280710478009 1 7 1.0224068110044726 1 8 0.923687525759405 1 9 0.9037191333757519 1 10 0.964274486391929 1 11 1.1038334510066932 1 12 0.9486469488743339 1 13 1.0171825781860306 1 14 1.0663338138498162 1 15 1.0148750174744592 1 16 1.1308935491529368 1 17 1.1552005659010334 1 18 1.194515939054952 1 19 1.0608424877893157 1 20 1.1018715479942998 1 21 0.9531730964457168 1 22 1.1727635808748005 1 23 0.9403563612885009 1 24 0.9843560196976782 1 25 1.060961169834826 1 26 1.0402508375582684 1 27 1.0228724721353686 1 28 1.0415899856025987 1 29 1.000779485284113 1 30 1.0042921495874104 1 31 0.999467237335729 1 32 0.9411662648817227 1 33 1.0136408992696992 1 34 0.9975737880447397 1 35 0.9235418492545229 1 36 1.070563828483575 1 37 0.9417074167827977 1 38 0.9961104725621794 1 39 0.9599814994532245 1 40 1.0736763509797669 1 41 0.9550158642333457 1 42 0.9342727888683022 1 43 0.9185281333220132 1 44 0.764476621489429 1 45 0.8944757793306906 1 46 0.8433331465526841 1 47 0.8693875051940125 1 48 0.9044001930012406 1 49 0.9233602648549528 1 50 0.8209405007892769 2 1 -29.12764988772615 2 2 -24.106852217652836 2 3 -23.942688573938593 2 4 -24.387486995171322 2 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2.083970633039222 119 14 1.9871727630240557 119 15 2.1286741075524134 119 16 2.0829193371592893 119 17 2.12791511666817 119 18 2.249776767921908 119 19 2.2395884370800303 119 20 2.2461477774447047 119 21 2.0657220808614696 119 22 2.1662369090065 119 23 2.265445258751555 119 24 2.318249938930066 119 25 2.069252283730698 119 26 2.2122823819638597 119 27 2.1397237684986834 119 28 2.1735168689121807 119 29 2.2633581135490743 119 30 2.2944210740376683 119 31 2.410149452934938 119 32 2.50257801229899 119 33 2.488565431132553 119 34 2.5590352405232366 119 35 2.629838781291422 119 36 2.7320385593419623 119 37 2.802585876806255 119 38 2.6618137709370657 119 39 2.6679899547671653 119 40 2.7354744763666226 119 41 2.7313487808258365 119 42 2.78119747323532 119 43 2.643876370642648 119 44 2.749872477894783 119 45 2.599692074254662 119 46 2.5622931635832877 119 47 2.593769454310209 119 48 2.6303438587806696 119 49 2.464911777567721 119 50 2.7469007560224306 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 927077.0 3 9695.0 4 15964.0 5 16042.0 6 31641.0 7 44829.0 8 54562.0 9 59561.0 10 78645.0 11 96660.0 12 105817.0 13 125911.0 14 143616.0 15 154931.0 16 163985.0 17 178308.0 18 200482.0 19 230157.0 20 269970.0 21 320433.0 22 374747.0 23 440227.0 24 515948.0 25 611846.0 26 710575.0 27 787371.0 28 819680.0 29 834972.0 30 952161.0 31 1096387.0 32 1274449.0 33 1507583.0 34 1829136.0 35 2278275.0 36 2849200.0 37 3472838.0 38 3357369.0 39 3906231.0 40 173.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 18.69067303763069 32.04652043018694 26.61578693497785 22.647019597204512 2 24.055299790751043 32.02166472927376 26.29225685455925 17.630778625415953 3 23.593693748465093 30.916426394332774 26.19210714713045 19.297772710071683 4 23.82133291663462 30.76209328401528 26.361480580158254 19.05509321919185 5 25.757402863782534 31.161025477175524 25.42803370417539 17.65353795486655 6 23.357867612534093 29.294024025063724 25.886108842960514 21.461999519441672 7 22.31473300843564 29.1976511254381 27.71171974192749 20.775896124198777 8 22.015484334936904 29.09832954014911 27.559371713057235 21.326814411856752 9 21.69642590276656 28.60487935244186 28.097365831228732 21.60132891356285 10 21.809587008552107 29.348634907935427 27.034294982035746 21.80748310147672 11 22.0770245738919 29.37407735497393 27.02271960596813 21.52617846516604 12 21.94010241493512 29.123748754111116 27.30910952975244 21.627039301201325 13 21.90143159365256 29.14135146333657 27.169831616177177 21.7873853268337 14 22.006558466705226 29.33046921797825 27.37680717507513 21.286165140241394 15 21.82851135785793 29.056965284720093 27.31892557486268 21.7955977825593 16 21.93666265909309 29.063260284236996 27.38688059785343 21.613196458816482 17 22.411502745088786 28.764260415138317 27.445931194191907 21.378305645580994 18 21.953549229703043 28.88193949490937 27.280082823042704 21.884428452344885 19 22.34039801145339 28.8644566906559 27.319826783759854 21.47531851413086 20 22.43032439565353 28.47855126066482 27.56404791137706 21.527076432304593 21 22.118104139161694 28.862605646482205 27.34696678695104 21.672323427405065 22 22.463445443504025 28.46911125955484 27.248504852296723 21.818938444644413 23 22.219022605631597 28.649629534970256 27.528697189388062 21.60265067001009 24 22.232428180396056 28.620501797506105 27.321535175084826 21.825534847013014 25 22.56852883375359 28.386225958904422 27.127566042298973 21.917679165043015 26 22.62763901202698 28.473908531811936 27.402499356007386 21.4959531001537 27 22.677794524224332 28.248233027962062 27.35093505448601 21.723037393327598 28 22.709287627612333 28.385174800097722 27.207123286161107 21.69841428612884 29 22.71170834676424 28.384754587225352 27.300060483258413 21.603476582751995 30 22.580979013170303 28.24349304461078 27.35664407856433 21.818883863654587 31 22.55965438440416 28.34517603414703 27.3329811478747 21.76218843357411 32 22.61124394439595 28.35545908159265 27.344749051449075 21.68854792256233 33 22.548246944239125 28.304830713454947 27.292375210828233 21.854547131477688 34 22.58014741414589 28.422195291686318 27.248312840913574 21.749344453254217 35 22.68317519453051 28.40441850343984 27.249240979148247 21.663165322881405 36 22.56996266932217 28.26267860440567 27.315809387357465 21.851549338914698 37 22.603722685373697 28.365042948360536 27.200079069007206 21.831155297258565 38 22.644841492306128 28.3500037374096 27.276046760184958 21.72910801009931 39 22.56431750624861 28.22094337468963 27.283426482500683 21.931312636561078 40 22.557828069333492 28.340497097584404 27.2390247416348 21.862650091447307 41 22.637186750391226 28.37734185539417 27.24669816434301 21.73877322987159 42 22.618214433128855 28.232371349727813 27.23387312408005 21.915541093063283 43 22.652323670079014 28.3257241599013 27.16459337298957 21.85735879703012 44 22.677369400262005 28.325570467356805 27.214649803147108 21.782410329234082 45 22.61378012889452 28.188475599864432 27.222883240291317 21.974861030949732 46 22.68714119031349 28.27653348301911 27.10629659073679 21.93002873593061 47 22.73124218653318 28.29312422709176 27.146807769478215 21.828825816896853 48 22.66383070453695 28.181575270813536 27.1455139564174 22.009080068232116 49 22.692314171307455 28.26700535764844 27.06202375435007 21.97865671669404 50 22.743383411642352 28.29204867084096 27.089124946713696 21.875442970803 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 15114.0 1 8940.5 2 2767.0 3 2369.5 4 1972.0 5 2093.0 6 2214.0 7 2997.0 8 3780.0 9 5330.0 10 6880.0 11 11078.5 12 15277.0 13 24151.5 14 33026.0 15 49382.0 16 65738.0 17 97119.5 18 128501.0 19 175531.0 20 222561.0 21 291954.5 22 361348.0 23 456550.0 24 551752.0 25 613298.5 26 674845.0 27 769157.5 28 863470.0 29 961255.5 30 1059041.0 31 1171279.5 32 1283518.0 33 1389143.5 34 1494769.0 35 1605971.5 36 1717174.0 37 1798850.0 38 1880526.0 39 1936905.0 40 1993284.0 41 2033935.0 42 2074586.0 43 2087266.0 44 2099946.0 45 2088338.0 46 2076730.0 47 2021603.0 48 1966476.0 49 1909282.0 50 1852088.0 51 1785313.5 52 1718539.0 53 1636852.5 54 1555166.0 55 1441947.0 56 1328728.0 57 1217612.5 58 1106497.0 59 990208.0 60 873919.0 61 754569.0 62 635219.0 63 537413.0 64 439607.0 65 367916.0 66 296225.0 67 240974.5 68 185724.0 69 148889.0 70 112054.0 71 89464.0 72 66874.0 73 52632.5 74 38391.0 75 29390.5 76 20390.0 77 15576.5 78 10763.0 79 8211.5 80 5660.0 81 4243.0 82 2826.0 83 2150.5 84 1475.0 85 1155.0 86 835.0 87 682.0 88 529.0 89 418.5 90 308.0 91 229.5 92 151.0 93 111.5 94 72.0 95 54.5 96 37.0 97 37.5 98 38.0 99 41.0 100 44.0 >>END_MODULE >>Per base N content warn #Base N-Count 1 7.2946441544251925 2 1.42835775036734 3 1.4082037370085712 4 0.732018272885665 5 0.0 6 6.4835172458641155E-6 7 0.0 8 6.483517245864116E-5 9 0.0 10 2.7230772432629285E-4 11 0.0 12 0.0 13 0.0038933521061414013 14 0.0 15 0.0 16 8.752748281916555E-5 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 2.5934068983456462E-5 24 0.0017797254839896997 25 0.004259670830532724 26 0.01526544135538706 27 0.020802365083355017 28 0.01785884825373271 29 0.024361816051334414 30 0.016568628321805747 31 0.024773519396446786 32 0.026079948121488405 33 0.03866121333708772 34 0.03806797150909116 35 0.03784429016410885 36 0.04657758789428781 37 0.060578743386731373 38 0.0776919871571897 39 0.08751451578467383 40 0.09452643968607588 41 0.0922636921672693 42 0.09911352813752473 43 0.11231396925010406 44 0.1195657832896031 45 0.12343320132676104 46 0.11171748566348458 47 0.11692050825329053 48 0.11531259597631623 49 0.12271677267109304 50 0.10627133117695872 >>END_MODULE >>Sequence Length Distribution pass #Length Count 50 3.0847454E7 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.01408959137488 #Duplication Level Percentage of deduplicated Percentage of total 1 82.19814057805333 49.33046572895719 2 10.04043292198108 12.051348818319251 3 3.3372626748143444 6.0084834346877845 4 1.4413122392078677 3.459961674118645 5 0.7272831188234683 2.1823617125683086 6 0.4435442108216298 1.597134120359098 7 0.29507477450644903 1.2396050767369342 8 0.21377803930165756 1.0263755522654512 9 0.1533315270986469 0.8281846804032468 >10 0.9735373115644911 11.518995330792716 >50 0.12386800949076097 5.157614914247207 >100 0.05174073961403387 5.093592877466039 >500 5.56903029818631E-4 0.21676937836560764 >1k 1.098247915638308E-4 0.093525743152856 >5k 1.6276140576824278E-5 0.056953821249077476 >10k+ 1.0850760258120165E-5 0.1386271363107285 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 3.2417586229320578E-6 0.0 0.0 0.0 0.0 2 3.2417586229320578E-6 0.0 0.0 3.2417586229320578E-6 0.0 3 6.4835172458641155E-6 0.0 0.0 6.4835172458641155E-6 0.0 4 6.4835172458641155E-6 6.4835172458641155E-6 0.0 6.4835172458641155E-6 0.0 5 6.4835172458641155E-6 6.4835172458641155E-6 0.0 9.725275868796173E-6 0.0 6 6.4835172458641155E-6 6.4835172458641155E-6 0.0 1.620879311466029E-5 3.2417586229320578E-6 7 6.4835172458641155E-6 1.2967034491728231E-5 0.0 1.9450551737592346E-5 3.2417586229320578E-6 8 6.4835172458641155E-6 1.2967034491728231E-5 0.0 2.5934068983456462E-5 3.2417586229320578E-6 9 1.2967034491728231E-5 1.620879311466029E-5 0.0 2.9175827606388522E-5 6.4835172458641155E-6 10 1.231868276714182E-4 1.620879311466029E-5 0.0 3.241758622932058E-5 6.4835172458641155E-6 11 1.231868276714182E-4 1.620879311466029E-5 0.0 4.2142862098116755E-5 6.4835172458641155E-6 12 1.4263737940901054E-4 2.2692310360524406E-5 0.0 4.2142862098116755E-5 6.4835172458641155E-6 13 1.6532968976953495E-4 2.2692310360524406E-5 0.0 4.862637934398087E-5 6.4835172458641155E-6 14 1.6857144839246702E-4 2.2692310360524406E-5 0.0 5.1868137966912924E-5 6.4835172458641155E-6 15 1.7829672426126317E-4 2.2692310360524406E-5 0.0 6.807693108157321E-5 6.4835172458641155E-6 16 1.8153848288419524E-4 2.2692310360524406E-5 0.0 7.780220695036938E-5 6.4835172458641155E-6 17 1.8802200013005936E-4 2.5934068983456462E-5 0.0 8.104396557330145E-5 6.4835172458641155E-6 18 2.074725518676517E-4 2.5934068983456462E-5 0.0 8.104396557330145E-5 6.4835172458641155E-6 19 2.2043958635937992E-4 2.5934068983456462E-5 0.0 9.401100006502968E-5 6.4835172458641155E-6 20 3.2741762091613783E-4 2.9175827606388522E-5 0.0 1.0373627593382585E-4 6.4835172458641155E-6 21 3.760440002601187E-4 2.9175827606388522E-5 0.0 1.1021979317968996E-4 6.4835172458641155E-6 22 4.1818686235823546E-4 2.9175827606388522E-5 0.0 1.1346155180262203E-4 6.4835172458641155E-6 23 4.797802761939446E-4 2.9175827606388522E-5 0.0 1.1346155180262203E-4 9.725275868796173E-6 24 5.510989658984498E-4 2.9175827606388522E-5 0.0 1.1994506904848614E-4 9.725275868796173E-6 25 6.418682073405475E-4 3.5659344852252635E-5 0.0 1.2967034491728231E-4 9.725275868796173E-6 26 7.099451384221207E-4 3.5659344852252635E-5 0.0 1.2967034491728231E-4 9.725275868796173E-6 27 8.687913109457915E-4 3.5659344852252635E-5 0.0 1.3615386216314643E-4 9.725275868796173E-6 28 0.0011573078283867446 3.890110347518469E-5 0.0 1.4263737940901054E-4 1.2967034491728231E-5 29 0.0014166485182213094 3.890110347518469E-5 0.0 1.5236265527780672E-4 1.620879311466029E-5 30 0.0017505496563833113 3.890110347518469E-5 0.0 1.5236265527780672E-4 1.620879311466029E-5 31 0.0024961541396576847 3.890110347518469E-5 0.0 1.5560441390073877E-4 1.620879311466029E-5 32 0.0037960993474534395 4.2142862098116755E-5 0.0 1.5560441390073877E-4 1.620879311466029E-5 33 0.012960550974482368 4.862637934398087E-5 0.0 1.5560441390073877E-4 1.9450551737592346E-5 34 0.013544067526610137 4.862637934398087E-5 0.0 1.5884617252367084E-4 1.9450551737592346E-5 35 0.013939562078607848 4.862637934398087E-5 0.0 1.620879311466029E-4 1.9450551737592346E-5 36 0.01430588080299917 4.862637934398087E-5 0.0 1.620879311466029E-4 1.9450551737592346E-5 37 0.01475972701020966 4.862637934398087E-5 0.0 1.6857144839246702E-4 1.9450551737592346E-5 38 0.015203847941551352 4.862637934398087E-5 0.0 1.6857144839246702E-4 1.9450551737592346E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATGCCG 1985 0.0 17.655197 16 TCGTATG 2020 0.0 16.590958 13 CGTATGC 2300 0.0 15.237204 14 CTCGTAT 2230 0.0 15.126806 12 GATCGGA 2700 0.0 13.194283 1 GCTCGTA 2725 0.0 12.780917 11 GAGTACT 20195 0.0 11.086292 20 GTACTTT 20940 0.0 10.598982 22 AAGCAGT 35060 0.0 10.413301 1 GAGTACA 26330 0.0 10.39022 17 AGTACTT 21520 0.0 10.333365 21 TAGATCG 1280 0.0 10.275551 32 GCCGTCT 3360 0.0 10.235176 19 GTACATG 26810 0.0 10.090323 19 ATCGGAA 3955 0.0 9.820932 2 TACATGG 27725 0.0 9.75041 20 AGTACAT 28675 0.0 9.494844 18 AGCAGTG 38970 0.0 9.483316 2 AGCTCGT 3900 0.0 9.323443 10 CCGTCTT 3830 0.0 9.323266 20 >>END_MODULE