FastQCFastQC Report
Thu 26 May 2016
SRR522132_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR522132_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41206039
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG28400.019.82169716
CGTATGC30650.018.36659814
TCGTATG31450.017.96936613
CTCGTAT32300.017.56456812
GATCGGA40650.016.7283461
AAGCAGT285750.013.3387981
TAGATCG16250.012.864614532
ATCGGAA53700.012.8182362
GCTCGTA50050.011.77477411
AGAGCGG38400.011.46803741
AGCAGTG346800.011.0782722
GCCGTCT51450.010.89959619
GAGCGGT33350.010.76187342
AGTGGTA413250.010.494272
TCAACGC410850.010.4852759
GTGGTAT411850.010.464893
CCGTCTT54450.010.46176320
ATCAACG420450.010.2510868
CAACGCA424250.010.19040510
GGTATCA435800.09.8799015
Produced by FastQC (version 0.11.5)