##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR522132_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 41206039 Sequences flagged as poor quality 0 Sequence length 50 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 37.289863701774394 39.0 37.0 40.0 33.0 40.0 2 36.9362604835665 39.0 36.0 40.0 31.0 40.0 3 36.95283832061606 39.0 36.0 40.0 31.0 40.0 4 36.93035258739623 39.0 36.0 40.0 31.0 40.0 5 36.96641708269994 39.0 36.0 40.0 31.0 40.0 6 37.04685818018082 39.0 36.0 40.0 32.0 40.0 7 36.97765623626187 39.0 36.0 40.0 31.0 40.0 8 36.929788568127115 39.0 36.0 40.0 31.0 40.0 9 36.901717731228665 39.0 36.0 40.0 31.0 40.0 10 36.83049787920649 39.0 36.0 40.0 31.0 40.0 11 36.90127806266455 39.0 36.0 40.0 31.0 40.0 12 36.80741495196857 39.0 36.0 40.0 31.0 40.0 13 36.75517248818796 39.0 36.0 40.0 31.0 40.0 14 36.6921768918386 39.0 36.0 40.0 31.0 40.0 15 36.599219958996784 39.0 35.0 40.0 30.0 40.0 16 36.59113391122112 38.0 36.0 40.0 31.0 40.0 17 36.47287777405637 38.0 35.0 40.0 30.0 40.0 18 36.358164394301525 38.0 35.0 40.0 30.0 40.0 19 36.264143588273555 38.0 35.0 40.0 30.0 40.0 20 36.35437953645581 38.0 35.0 40.0 30.0 40.0 21 36.41868190728063 38.0 36.0 40.0 30.0 40.0 22 36.28934676298297 38.0 35.0 40.0 30.0 40.0 23 36.1963231166189 38.0 35.0 40.0 30.0 40.0 24 36.09906865835855 38.0 35.0 40.0 29.0 40.0 25 36.15652710516534 38.0 35.0 40.0 30.0 40.0 26 36.14820878561029 39.0 35.0 40.0 30.0 40.0 27 36.038694085592645 38.0 35.0 40.0 30.0 40.0 28 35.95089896895938 38.0 35.0 40.0 29.0 40.0 29 35.84817652577575 38.0 35.0 40.0 29.0 40.0 30 35.74511257925082 38.0 35.0 40.0 29.0 40.0 31 35.62143420288468 38.0 35.0 40.0 29.0 40.0 32 35.46365157786702 38.0 35.0 40.0 28.0 40.0 33 35.35999385915254 38.0 35.0 40.0 28.0 40.0 34 35.26375760116132 38.0 35.0 40.0 28.0 40.0 35 35.10735962755362 38.0 35.0 40.0 27.0 40.0 36 34.97693689995294 38.0 35.0 40.0 27.0 40.0 37 34.788973844343545 38.0 35.0 40.0 27.0 40.0 38 34.674766337041035 38.0 34.0 40.0 26.0 40.0 39 34.51959546512102 38.0 34.0 39.0 26.0 40.0 40 34.387590420908936 38.0 33.0 39.0 26.0 40.0 41 34.245789846483426 38.0 34.0 39.0 25.0 40.0 42 34.052703949535164 38.0 33.0 39.0 24.0 40.0 43 33.91897029947479 38.0 33.0 39.0 24.0 40.0 44 33.73565491213557 38.0 33.0 39.0 23.0 40.0 45 33.59325054271778 38.0 33.0 39.0 23.0 40.0 46 33.374646614298456 38.0 33.0 39.0 21.0 40.0 47 33.2295921721571 38.0 33.0 39.0 21.0 40.0 48 33.02603011660499 37.0 33.0 39.0 20.0 40.0 49 32.87742845168884 37.0 33.0 39.0 19.0 40.0 50 32.718303935983755 37.0 33.0 39.0 17.0 40.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 -0.301254188638012 1 2 -0.288635337135446 1 3 -0.2929878304811666 1 4 -0.3620057017214151 1 5 -0.3351212842587614 1 6 -0.43544019468588857 1 7 -0.2939944270871351 1 8 -0.40393185900266104 1 9 -0.37448473432951346 1 10 -0.33749290704627555 1 11 -0.23878447452144513 1 12 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226925.0 23 267020.0 24 309092.0 25 364886.0 26 438806.0 27 520070.0 28 602258.0 29 698906.0 30 820683.0 31 959194.0 32 1135811.0 33 1393975.0 34 1793226.0 35 2422331.0 36 3419203.0 37 5137507.0 38 6670849.0 39 1.2376665E7 40 2684.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 19.793514515833273 30.561866272401645 26.06577385224518 23.578845359519907 2 24.064280184264152 30.293822168631994 26.622620819564002 19.01927682753985 3 23.42362001841526 28.995417395008534 26.707645935101894 20.873316651474312 4 24.146247107129128 28.63518864310156 26.420207484635927 20.798356765133384 5 24.864649086994266 29.44709876142184 26.422474142685736 19.265778008898163 6 23.903054598380592 27.949944424408272 25.682349618705164 22.46465135850597 7 23.1239794730088 27.940377865487143 27.27384206960538 21.66180059189868 8 22.596760635012746 27.94292603567162 27.227023689416008 22.233289639899628 9 22.34290448825182 27.543217675659314 27.66254253415868 22.451335301930186 10 22.526576798296492 28.27375467360972 26.59380096279843 22.60586756529536 11 22.728924518252967 28.196431865854755 26.719798460232525 22.354845155659756 12 22.62502833625916 28.011556752640065 26.905862026680115 22.457552884420657 13 22.554727863622585 28.040730784952945 26.88541519944093 22.51912615198354 14 22.60308495072773 28.170890194031994 27.096200632145205 22.129824223095067 15 22.506517431561537 27.867098975531086 27.022608558314747 22.60377503459263 16 22.606552625215514 27.93828524677912 27.09419607455604 22.36096605344932 17 22.954211225063666 27.725257157701016 27.173355031124323 22.147176586110998 18 22.595285123134502 27.764372110602526 27.025446440023025 22.614896326239947 19 22.91589346891605 27.78468224038714 27.073820417439297 22.22560387325751 20 22.923967528157704 27.477503964892136 27.286442164460407 22.31208634248975 21 22.686694069510654 27.771317544445413 27.079689138037423 22.462299248006513 22 22.98140682638323 27.460614569656382 26.988239611588956 22.569738992371434 23 22.775492659574315 27.6711960487103 27.225178359388487 22.3281329323269 24 22.828397662684168 27.529749578816258 27.043074526860416 22.59877823163915 25 23.05972901315399 27.421169068080903 26.910639043516447 22.60846287524866 26 23.142321447738322 27.541821290862817 27.076243533891738 22.23961372750713 27 23.15865404828029 27.281508446667107 27.024181410681326 22.535656094371273 28 23.22388473254566 27.43639027913245 26.876433186814285 22.463291801507605 29 23.193156871488945 27.490065352429756 26.969722339620333 22.347055436460966 30 23.070768804191484 27.31396556589282 27.011006120401177 22.604259509514527 31 23.071785385754097 27.431721045989182 26.969669797498298 22.52682377075842 32 23.07731089393599 27.4742113916559 27.011702726597083 22.436774987811027 33 23.046490469293445 27.375970181527336 26.916952361725183 22.660586987454035 34 23.123796363119776 27.506264780438162 26.8553010079313 22.514637848510766 35 23.143695684440686 27.539066757888424 26.871819492004327 22.44541806566656 36 23.029479943750616 27.38908143973327 26.920093811120978 22.66134480539514 37 23.124841964866146 27.47227893182308 26.805424690877572 22.5974544124332 38 23.147630024186356 27.485209128169714 26.867548071213776 22.49961277643015 39 23.064723866980604 27.32543616122738 26.876269842480504 22.733570129311513 40 23.092557343162 27.4728369829348 26.795994205423835 22.63861146847937 41 23.11046794779087 27.510500807675957 26.86410984956595 22.514921394967217 42 23.116348435439992 27.362374526913918 26.80375630629622 22.717520731349875 43 23.16331508043015 27.434700444150145 26.74821590937858 22.653768566041126 44 23.14192411097734 27.444508293414227 26.836300285619526 22.57726730998891 45 23.07872606044286 27.315093409247197 26.82334058876257 22.782839941547373 46 23.216493999711542 27.42696885619173 26.657905514651713 22.698631629445014 47 23.208595147314128 27.437099285265205 26.72293061384038 22.631374953580288 48 23.123026201454028 27.283650128736436 26.752924076030315 22.84039959377922 49 23.191033933880707 27.374380908378875 26.65905256005403 22.775532597686393 50 23.163651077142024 27.443505753556046 26.707444633008627 22.6853985362933 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 12935.0 1 7944.5 2 2954.0 3 2659.5 4 2365.0 5 2516.0 6 2667.0 7 3432.0 8 4197.0 9 5942.0 10 7687.0 11 12875.0 12 18063.0 13 27922.5 14 37782.0 15 55566.0 16 73350.0 17 106411.5 18 139473.0 19 191788.0 20 244103.0 21 320784.5 22 397466.0 23 503797.0 24 610128.0 25 679599.0 26 749070.0 27 861452.0 28 973834.0 29 1086828.5 30 1199823.0 31 1337244.5 32 1474666.0 33 1611538.5 34 1748411.0 35 1906394.0 36 2064377.0 37 2175802.5 38 2287228.0 39 2383604.0 40 2479980.0 41 2562241.5 42 2644503.0 43 2693984.5 44 2743466.0 45 2753055.0 46 2762644.0 47 2699340.5 48 2636037.0 49 2611260.5 50 2586484.0 51 2540246.0 52 2494008.0 53 2412417.0 54 2330826.0 55 2192037.5 56 2053249.0 57 1906778.5 58 1760308.0 59 1603789.5 60 1447271.0 61 1264842.5 62 1082414.0 63 922469.5 64 762525.0 65 649180.5 66 535836.0 67 440541.0 68 345246.0 69 278438.5 70 211631.0 71 170977.0 72 130323.0 73 101822.5 74 73322.0 75 54989.5 76 36657.0 77 27801.5 78 18946.0 79 14133.5 80 9321.0 81 6964.5 82 4608.0 83 3517.0 84 2426.0 85 1956.0 86 1486.0 87 1223.0 88 960.0 89 730.0 90 500.0 91 346.0 92 192.0 93 161.5 94 131.0 95 97.5 96 64.0 97 52.5 98 41.0 99 48.0 100 55.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.06545157130972962 2 0.008901607844423 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 9.707314988465647E-5 10 0.0017885727866247955 11 1.868658135279637E-4 12 0.0 13 3.761584558030438E-4 14 0.0 15 1.4560972482698471E-5 16 4.926462356646316E-4 17 2.4268287471164115E-6 18 0.0 19 0.0 20 0.0 21 2.354023884702919E-4 22 4.853657494232823E-6 23 1.2134143735582058E-4 24 0.003247096863641759 25 0.005324462271173407 26 0.018844325221358937 27 0.022897129229043345 28 0.01219481445425997 29 0.023200482822432898 30 0.012486033903913939 31 0.0236445924831552 32 0.021775934347875562 33 0.0349269193284994 34 0.03071394462350531 35 0.03424983410805392 36 0.03685624818245695 37 0.049936855129414405 38 0.06862343648221077 39 0.09112013896797991 40 0.0936173457487627 41 0.09052313909618928 42 0.09620191836444168 43 0.10855933034475845 44 0.12107205936489067 45 0.1262678997124669 46 0.11976642549894204 47 0.11880540131508394 48 0.11440070713906764 49 0.12127833980839556 50 0.10703285506282222 >>END_MODULE >>Sequence Length Distribution pass #Length Count 50 4.1206039E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 42.60691851135044 #Duplication Level Percentage of deduplicated Percentage of total 1 71.22082830337952 30.345000278329724 2 15.19574180361488 12.948874654922813 3 5.594430696680003 7.150843584325271 4 2.5066318563020284 4.271994369576626 5 1.3361652759096676 2.8464942514189646 6 0.7837268768759887 2.0035312306926256 7 0.5158352470811178 1.5384705236367255 8 0.36459502525677 1.2427416424607165 9 0.2890272064799893 1.108310277065055 >10 1.7602353537386923 15.150489335810017 >50 0.2557914297210847 7.6884783699363375 >100 0.17433851947389853 12.817325544790375 >500 0.0025283039683180624 0.6606841460191928 >1k 9.564370659157866E-5 0.0592234398226813 >5k 1.7074686779083232E-5 0.06434978126499127 >10k+ 1.1383124516015331E-5 0.10318856992814673 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 1.698780122981488E-5 2.4268287471164115E-6 0.0 0.0 2.4268287471164115E-6 2 1.9414629976931292E-5 4.853657494232823E-6 0.0 2.4268287471164115E-6 4.853657494232823E-6 3 2.1841458724047706E-5 4.853657494232823E-6 0.0 4.853657494232823E-6 4.853657494232823E-6 4 2.1841458724047706E-5 7.2804862413492355E-6 0.0 4.853657494232823E-6 4.853657494232823E-6 5 2.4268287471164117E-5 9.707314988465646E-6 0.0 4.853657494232823E-6 7.2804862413492355E-6 6 2.9121944965396942E-5 9.707314988465646E-6 0.0 4.853657494232823E-6 2.1841458724047706E-5 7 3.154877371251335E-5 9.707314988465646E-6 0.0 4.853657494232823E-6 2.4268287471164117E-5 8 3.6402431206746174E-5 9.707314988465646E-6 0.0 9.707314988465646E-6 2.6695116218280528E-5 9 4.8536574942328234E-5 1.698780122981488E-5 0.0 9.707314988465646E-6 2.6695116218280528E-5 10 3.591706545732289E-4 1.698780122981488E-5 0.0 1.4560972482698471E-5 2.9121944965396942E-5 11 3.737316270559274E-4 1.9414629976931292E-5 0.0 1.698780122981488E-5 2.9121944965396942E-5 12 3.761584558030438E-4 1.9414629976931292E-5 0.0 1.698780122981488E-5 2.9121944965396942E-5 13 3.7858528455016026E-4 2.1841458724047706E-5 0.0 1.698780122981488E-5 2.9121944965396942E-5 14 3.8101211329727663E-4 2.1841458724047706E-5 0.0 2.4268287471164117E-5 2.9121944965396942E-5 15 3.907194282857423E-4 2.4268287471164117E-5 0.0 3.154877371251335E-5 3.154877371251335E-5 16 3.907194282857423E-4 2.4268287471164117E-5 0.0 3.154877371251335E-5 3.397560245962976E-5 17 3.9799991452709154E-4 2.4268287471164117E-5 0.0 3.8829259953862585E-5 3.6402431206746174E-5 18 4.149877157569064E-4 2.4268287471164117E-5 0.0 4.6109746195211823E-5 4.1256088700979E-5 19 4.2712185949248844E-4 2.6695116218280528E-5 0.0 5.0963403689444645E-5 4.368291744809541E-5 20 4.562438044578854E-4 3.397560245962976E-5 0.0 5.581706118367747E-5 4.368291744809541E-5 21 4.708047769405839E-4 3.6402431206746174E-5 0.0 5.581706118367747E-5 4.368291744809541E-5 22 5.047803794002136E-4 3.6402431206746174E-5 0.0 5.581706118367747E-5 4.8536574942328234E-5 23 5.533169543425418E-4 3.6402431206746174E-5 0.0 5.8243889930793884E-5 4.8536574942328234E-5 24 5.897193855492881E-4 3.6402431206746174E-5 0.0 6.552437617214312E-5 4.8536574942328234E-5 25 6.455364467329656E-4 3.6402431206746174E-5 0.0 6.552437617214312E-5 5.3390232436561056E-5 26 6.989266791695266E-4 3.6402431206746174E-5 0.0 6.552437617214312E-5 5.581706118367747E-5 27 7.571705691003205E-4 3.6402431206746174E-5 0.0 6.795120491925953E-5 5.8243889930793884E-5 28 8.493900614907441E-4 3.6402431206746174E-5 2.4268287471164115E-6 7.280486241349235E-5 5.8243889930793884E-5 29 0.0010411095325129406 3.8829259953862585E-5 2.4268287471164115E-6 7.280486241349235E-5 6.30975474250267E-5 30 0.0012304021747880207 3.8829259953862585E-5 2.4268287471164115E-6 7.280486241349235E-5 7.280486241349235E-5 31 0.0016308289180622286 3.8829259953862585E-5 2.4268287471164115E-6 7.523169116060877E-5 7.523169116060877E-5 32 0.0030311091051483984 4.1256088700979E-5 2.4268287471164115E-6 7.765851990772517E-5 7.765851990772517E-5 33 0.016267033091921308 4.1256088700979E-5 2.4268287471164115E-6 7.765851990772517E-5 7.765851990772517E-5 34 0.016820350046263848 4.8536574942328234E-5 2.4268287471164115E-6 7.765851990772517E-5 7.765851990772517E-5 35 0.016985374401067767 5.0963403689444645E-5 2.4268287471164115E-6 8.008534865484159E-5 8.008534865484159E-5 36 0.017150398755871682 5.0963403689444645E-5 2.4268287471164115E-6 8.736583489619083E-5 8.2512177401958E-5 37 0.017378520658100624 5.0963403689444645E-5 2.4268287471164115E-6 8.736583489619083E-5 8.2512177401958E-5 38 0.017592081587846867 5.581706118367747E-5 2.4268287471164115E-6 8.979266364330723E-5 8.736583489619083E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATGCCG 2840 0.0 19.821697 16 CGTATGC 3065 0.0 18.366598 14 TCGTATG 3145 0.0 17.969366 13 CTCGTAT 3230 0.0 17.564568 12 GATCGGA 4065 0.0 16.728346 1 AAGCAGT 28575 0.0 13.338798 1 TAGATCG 1625 0.0 12.8646145 32 ATCGGAA 5370 0.0 12.818236 2 GCTCGTA 5005 0.0 11.774774 11 AGAGCGG 3840 0.0 11.468037 41 AGCAGTG 34680 0.0 11.078272 2 GCCGTCT 5145 0.0 10.899596 19 GAGCGGT 3335 0.0 10.761873 42 AGTGGTA 41325 0.0 10.49427 2 TCAACGC 41085 0.0 10.485275 9 GTGGTAT 41185 0.0 10.46489 3 CCGTCTT 5445 0.0 10.461763 20 ATCAACG 42045 0.0 10.251086 8 CAACGCA 42425 0.0 10.190405 10 GGTATCA 43580 0.0 9.879901 5 >>END_MODULE