FastQCFastQC Report
Thu 26 May 2016
SRR522131_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR522131_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32464829
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT573150.1765449003289067No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG25150.023.95533813
TATGCCG27550.021.63242516
CGTATGC29500.020.64323614
AAGCAGT277350.020.6296621
CTCGTAT29900.019.93228112
GATCGGA40200.016.941391
AGCAGTG349450.016.4174062
GTACTTT240850.016.41055722
GAGTACT246450.016.29392620
AGTACTT249700.015.93205221
TAGATCG16500.014.850677532
TCAACGC379450.014.63826312
GTGGTAT383800.014.5397546
CAACGCA381500.014.49711713
ATCAACG385550.014.41230511
AGTGGTA389700.014.3474515
GGTATCA398000.014.0536048
AACGCAG401250.013.76728414
ATCGGAA49450.013.7625532
TGGTATC408650.013.7138617
Produced by FastQC (version 0.11.5)