##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR522131_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 32464829 Sequences flagged as poor quality 0 Sequence length 50 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 38.34397566055253 40.0 38.0 40.0 35.0 40.0 2 38.026080038801375 40.0 38.0 40.0 35.0 40.0 3 38.09301435100736 40.0 38.0 40.0 35.0 40.0 4 38.0609307383076 40.0 38.0 40.0 35.0 40.0 5 37.94355753421649 40.0 38.0 40.0 35.0 40.0 6 38.08427095057239 40.0 38.0 40.0 35.0 40.0 7 38.18254502434003 40.0 38.0 40.0 35.0 40.0 8 38.158737383153934 40.0 38.0 40.0 35.0 40.0 9 38.139567653351875 40.0 38.0 40.0 35.0 40.0 10 38.08768827952243 40.0 38.0 40.0 35.0 40.0 11 38.20193841156533 40.0 38.0 40.0 35.0 40.0 12 38.14919142189229 39.0 38.0 40.0 35.0 40.0 13 38.10786254256876 39.0 38.0 40.0 35.0 40.0 14 38.058862315276635 39.0 38.0 40.0 35.0 40.0 15 37.95964919451755 39.0 38.0 40.0 34.0 40.0 16 37.96556399542409 39.0 38.0 40.0 35.0 40.0 17 37.875365830511534 39.0 38.0 40.0 34.0 40.0 18 37.773643378808494 39.0 38.0 40.0 33.0 40.0 19 37.90471340539018 39.0 38.0 40.0 34.0 40.0 20 37.85747600888334 39.0 38.0 40.0 34.0 40.0 21 37.35940367959431 39.0 37.0 40.0 33.0 40.0 22 37.298584015335486 39.0 37.0 40.0 33.0 40.0 23 34.911320493941304 39.0 36.0 40.0 24.0 40.0 24 37.16597151951732 39.0 37.0 40.0 32.0 40.0 25 37.112017161710604 39.0 37.0 40.0 32.0 40.0 26 36.91838053420827 39.0 36.0 40.0 31.0 40.0 27 36.940290429375125 39.0 36.0 40.0 31.0 40.0 28 36.79930351704609 39.0 36.0 40.0 31.0 40.0 29 36.792662360858266 39.0 36.0 40.0 31.0 40.0 30 36.71697038662979 39.0 36.0 40.0 31.0 40.0 31 37.258661642727276 39.0 38.0 40.0 33.0 40.0 32 37.164552630170945 39.0 38.0 40.0 33.0 40.0 33 37.072283916850445 39.0 38.0 40.0 33.0 40.0 34 37.00642301242369 39.0 37.0 40.0 33.0 40.0 35 36.884733999368976 39.0 37.0 40.0 32.0 40.0 36 36.77007533290873 39.0 37.0 40.0 32.0 40.0 37 36.63626486373916 39.0 37.0 40.0 32.0 40.0 38 36.551432228397076 39.0 37.0 40.0 31.0 40.0 39 36.44321801294564 39.0 36.0 40.0 31.0 40.0 40 36.33769969957334 39.0 36.0 40.0 31.0 40.0 41 36.091601129332915 39.0 36.0 40.0 31.0 40.0 42 35.96326369068508 39.0 36.0 40.0 30.0 40.0 43 35.84594959671588 39.0 36.0 40.0 30.0 40.0 44 35.713614139165806 39.0 36.0 40.0 30.0 40.0 45 35.225197705492306 39.0 35.0 40.0 29.0 40.0 46 35.34442895109658 39.0 35.0 40.0 29.0 40.0 47 35.22827278714451 39.0 35.0 40.0 29.0 40.0 48 35.09671410867434 38.0 35.0 40.0 28.0 40.0 49 34.973387508062956 38.0 35.0 40.0 28.0 40.0 50 34.842550780107295 38.0 35.0 40.0 28.0 40.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 0.1862553948793959 1 2 0.3559410406732795 1 3 0.2934711005557702 1 4 0.2319742728194072 1 5 0.3721754285540513 1 6 0.33657968170904695 1 7 0.07833994916873621 1 8 0.014798426097094364 1 9 0.0615949447399089 1 10 0.06764001931819053 1 11 -0.08879828329003203 1 12 -0.06859579415151984 1 13 -0.1370280349242634 1 14 -0.03484324027670738 1 15 0.01609836416542265 1 16 -0.0038922477850817927 1 17 -0.03267812643935031 1 18 0.03736548862790556 1 19 -0.07154531414363419 1 20 -0.061825635234782794 1 21 0.5051182176959159 1 22 0.4617888863301687 1 23 2.810089626920117 1 24 0.4344407810277602 1 25 0.41523852561123675 1 26 0.5213648561341273 1 27 0.5140714213857507 1 28 0.6087734429762648 1 29 0.43336727312042456 1 30 0.4749766762658041 1 31 -0.23726554169311953 1 32 -0.23084455841322438 1 33 -0.26589569719354245 1 34 -0.23361237167001292 1 35 -0.2879843585514905 1 36 -0.20841028740683498 1 37 -0.45653340391759656 1 38 -0.23267453077507838 1 39 -0.16326601553694786 1 40 -0.1679555888333013 1 41 0.07056824329746547 1 42 0.05755813033671586 1 43 0.04668535041571431 1 44 0.11922025504556899 1 45 0.4918876988968748 1 46 0.0994079070561682 1 47 0.10748981694758442 1 48 0.09216902229723445 1 49 0.09372821366741135 1 50 0.062411538326436755 2 1 0.0901004472013085 2 2 0.17648163960771512 2 3 0.15135253378878133 2 4 0.20777571084507684 2 5 0.30037115548816473 2 6 0.1947182840024837 2 7 0.002285316374127433 2 8 -0.02134235553628372 2 9 -0.017260048502471648 2 10 -0.03306460479225137 2 11 -0.2614182510696921 2 12 -0.1493999731191309 2 13 -0.13400229550014586 2 14 -0.12297476105811711 2 15 0.017257412554336327 2 16 0.02981824197935623 2 17 0.011351669811055842 2 18 0.021331701547893545 2 19 0.001109891756030379 2 20 -0.019970486282112176 2 21 0.5130635369388941 2 22 0.45741665005980536 2 23 2.821426786010285 2 24 0.4766974169606115 2 25 0.47634535986668425 2 26 0.5453430019817134 2 27 0.5833468382187448 2 28 0.6154797229840767 2 29 0.5337794430886262 2 30 0.41723274488099094 2 31 -0.14560407673628362 2 32 -0.13786626751262077 2 33 -0.14260456288609902 2 34 -0.1504891979862748 2 35 -0.2562606334076136 2 36 -0.17920064912154032 2 37 -0.13323616622552237 2 38 -0.15397959356178603 2 39 -0.09976223938421924 2 40 -0.05865205258420758 2 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0.482868126391125 120 23 2.8366027428081466 120 24 0.4683134625523877 120 25 0.4259188922193715 120 26 0.5387700468540331 120 27 0.6371922812785442 120 28 0.7580333265146209 120 29 0.6984348271201029 120 30 0.6686900689617516 120 31 0.13599725504882798 120 32 0.09737337808563495 120 33 0.1577917851884223 120 34 0.12892981518294988 120 35 0.14751867051595013 120 36 0.1926059768824402 120 37 0.16468229532276268 120 38 0.12366238748921177 120 39 0.046238793757027 120 40 0.03362094714064057 120 41 0.17098299198620737 120 42 0.21720154694752125 120 43 0.08990751362915717 120 44 0.20588908684970164 120 45 0.5112053210123548 120 46 0.2009794301714649 120 47 0.3555616578290568 120 48 0.37172778066670276 120 49 0.22099883608530746 120 50 0.29328426281382036 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 36430.0 3 1941.0 4 3151.0 5 3421.0 6 4849.0 7 9505.0 8 11796.0 9 12331.0 10 17793.0 11 26259.0 12 26057.0 13 32611.0 14 42684.0 15 50362.0 16 50567.0 17 53278.0 18 65321.0 19 77645.0 20 81823.0 21 82137.0 22 101965.0 23 124208.0 24 138133.0 25 158166.0 26 195676.0 27 233438.0 28 268617.0 29 309993.0 30 376557.0 31 457262.0 32 562214.0 33 725570.0 34 998465.0 35 1451478.0 36 2249897.0 37 3739920.0 38 5543854.0 39 1.4119521E7 40 19934.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 19.53770486052575 29.49259467810141 25.84729515380806 25.122405307564787 2 24.007268177583306 28.84938666472464 26.74377098617271 20.39957417151934 3 23.422661841695096 27.264905847752186 26.837955621597047 22.47447668895567 4 23.81779987197838 26.96988485600833 26.545095925193387 22.66721934681991 5 24.581065195673453 27.982166641711416 26.57534348688118 20.86142467573395 6 24.56402351969261 27.002791646683427 25.443449209926715 22.98973562369724 7 23.75887831202707 27.05153516182118 26.964000317143626 22.225586209008124 8 23.329923179280062 27.14557784707743 26.736467169728307 22.7880318039142 9 23.123707447014954 26.72708488757985 27.188806221626983 22.96040144377822 10 23.0208420318493 27.417350635051857 26.431754807641216 23.130052525457625 11 23.225005067794786 27.58029609351491 26.306630893369437 22.88806794532086 12 23.11628121018957 27.241654175701324 26.65899538324557 22.983069230863535 13 23.081036193958486 27.304332590771054 26.396498070499337 23.218133144771123 14 23.17266479364484 27.546746049393946 26.597158420270752 22.683430736690465 15 23.116837545053407 27.29100412865576 26.49723374273259 23.094924583558242 16 23.153973451539244 27.196855109343005 26.58276381558323 23.066407623534523 17 23.66555203478817 27.019455423590866 26.62660259199271 22.688389949628256 18 23.122293359376698 27.090070303465946 26.49200770470715 23.295628632450214 19 23.417756941954355 27.27111041730011 26.58418516071539 22.726947480030148 20 23.6212918293825 26.825962952091942 26.753814104488278 22.798931114037284 21 23.226401900263987 27.26079117333613 26.544810026890257 22.967996899509625 22 23.66433483977439 26.817338905683652 26.462588715654856 23.055737538887097 23 23.300420101033406 27.002067010015633 26.868734589933315 22.82877829901765 24 23.35580713550278 27.05220981264473 26.480491687260166 23.111491364592325 25 23.5155335390107 26.7835796332893 26.418510778193273 23.28237604950673 26 23.606391978326595 26.87179259680974 26.770394139752053 22.75142128511161 27 23.62350773850343 26.626468099902347 26.701687140119716 23.048337021474506 28 23.668815575900386 26.759474243740954 26.574704195101695 22.997005985256965 29 23.67843780731568 26.8385549785449 26.629857590373916 22.8531496237655 30 23.56492260169502 26.683176851017347 26.64356337552281 23.108337171764816 31 23.532178015036013 26.805021158117754 26.644713480760323 23.018087346085913 32 23.53610098520521 26.839361782465353 26.66720606224763 22.95733117008181 33 23.509870363384387 26.77055997418613 26.55030889843618 23.1692607639933 34 23.552524108871665 26.89348104318271 26.520259512393334 23.03373533555229 35 23.58308006233374 26.928025965879137 26.516557719026174 22.97233625276095 36 23.474303009460844 26.786540349553768 26.58072345143515 23.158433189550244 37 23.56989049691297 26.871670014525467 26.468081075377608 23.090358413183957 38 23.61155161635475 26.86764000499818 26.523617481870037 22.99719089677703 39 23.53839530171967 26.729518470411417 26.506425004274252 23.225661223594663 40 23.541943636971915 26.848992203171584 26.450571151253932 23.15849300860257 41 23.58051239280269 26.89912541648944 26.497136940004957 23.023225250702914 42 23.589867841219554 26.7526436288051 26.43371069055193 23.223777839423416 43 23.620328047727 26.84822192498076 26.375366058761244 23.156083968530993 44 23.613892082560405 26.808745680834235 26.464751179170953 23.112611057434403 45 23.527332406496303 26.73025055573736 26.429474808112886 23.312942229653455 46 23.68146331011447 26.818038896127693 26.28273636938801 23.217761424369826 47 23.68212086767366 26.832122132018576 26.35017235179873 23.135584648509028 48 23.599293936971748 26.693000541536183 26.384122635219537 23.32358288627253 49 23.636732731113263 26.791548377256685 26.295727359066177 23.275991532563875 50 23.607453792598527 26.841342871154072 26.347953302206072 23.20325003404133 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 23264.0 1 13439.0 2 3614.0 3 3098.5 4 2583.0 5 2622.0 6 2661.0 7 3243.0 8 3825.0 9 5235.0 10 6645.0 11 9971.5 12 13298.0 13 20321.0 14 27344.0 15 38967.0 16 50590.0 17 74937.5 18 99285.0 19 135888.5 20 172492.0 21 226565.0 22 280638.0 23 373295.5 24 465953.0 25 491673.0 26 517393.0 27 595738.5 28 674084.0 29 754948.0 30 835812.0 31 934499.0 32 1033186.0 33 1136713.5 34 1240241.0 35 1359038.0 36 1477835.0 37 1568687.0 38 1659539.0 39 1743581.0 40 1827623.0 41 1901968.0 42 1976313.0 43 2025156.0 44 2073999.0 45 2101984.0 46 2129969.0 47 2102208.0 48 2074447.0 49 2077434.5 50 2080422.0 51 2063125.0 52 2045828.0 53 2001509.5 54 1957191.0 55 1860031.5 56 1762872.0 57 1654754.5 58 1546637.0 59 1424278.5 60 1301920.0 61 1151400.0 62 1000880.0 63 863934.0 64 726988.0 65 625703.0 66 524418.0 67 435860.5 68 347303.0 69 280846.0 70 214389.0 71 173921.5 72 133454.0 73 104140.0 74 74826.0 75 55732.0 76 36638.0 77 27541.5 78 18445.0 79 13559.5 80 8674.0 81 6549.0 82 4424.0 83 3546.0 84 2668.0 85 2264.0 86 1860.0 87 1524.0 88 1188.0 89 862.5 90 537.0 91 388.5 92 240.0 93 184.0 94 128.0 95 108.0 96 88.0 97 85.0 98 82.0 99 89.0 100 96.0 >>END_MODULE >>Per base N content warn #Base N-Count 1 0.07404320534077047 2 0.01192675310256524 3 0.001198219771926105 4 0.0 5 1.601733371212274E-4 6 3.6655052148896274E-4 7 1.3245102877332266E-4 8 6.160512966201053E-5 9 2.7722308347904742E-5 10 0.0 11 5.5444616695809483E-5 12 9.548795097611634E-5 13 6.530143744173118E-4 14 0.0 15 7.700641207751317E-5 16 0.00385648111684186 17 0.0 18 0.0 19 4.928410372960844E-5 20 0.0 21 8.840336106498512E-4 22 3.6963077797206325E-5 23 7.8088321364637405 24 0.003400603157342982 25 0.004201469842949119 26 0.014689743167906413 27 0.019380973791668518 28 0.010469791786058691 29 0.019556548411205243 30 0.011252176932766225 31 0.026157538054489674 32 0.02694608371416341 33 0.033753450541815576 34 0.027839358094262565 35 0.03627926085795801 36 0.04056389762595084 37 0.0438536115499022 38 0.06475623204422239 39 0.08134033294923562 40 0.08482102277513921 41 0.0841895701961036 42 0.08791976079713834 43 0.10021922493415876 44 0.11123730237420934 45 1.6470993886953789 46 0.11626428095462939 47 0.11387400192374338 48 0.10738698177033368 49 0.11272198599906377 50 0.10368451347764684 >>END_MODULE >>Sequence Length Distribution pass #Length Count 50 3.2464829E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 43.20382914609472 #Duplication Level Percentage of deduplicated Percentage of total 1 71.5851042407453 30.927506130225403 2 15.49603735527462 13.389763006775723 3 5.355111635949906 6.940839845335304 4 2.2252056915863125 3.845496260568503 5 1.193736258630182 2.578698868167836 6 0.6796986890210417 1.7619351618773795 7 0.4630203395389148 1.4002976148424229 8 0.3475505150246002 1.2012410456608043 9 0.28462086217170507 1.1067039990612468 >10 1.931074794752428 17.213086372648583 >50 0.2978847331733221 9.041907078443424 >100 0.13990778778870117 10.00595868471984 >500 9.749843315693781E-4 0.25427912015491166 >1k 3.625190942556614E-5 0.03146354839911433 >5k 2.151606121839413E-5 0.06422442191873136 >10k+ 1.434404080299659E-5 0.23659884120085417 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 57315 0.1765449003289067 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 6.160512966201054E-6 9.240769449301581E-6 0.0 0.0 0.0 2 2.156179538170369E-5 9.240769449301581E-6 0.0 0.0 0.0 3 2.156179538170369E-5 9.240769449301581E-6 0.0 0.0 0.0 4 2.156179538170369E-5 9.240769449301581E-6 0.0 0.0 3.080256483100527E-6 5 2.7722308347904742E-5 1.2321025932402108E-5 0.0 0.0 3.080256483100527E-6 6 2.7722308347904742E-5 1.2321025932402108E-5 0.0 3.080256483100527E-6 3.080256483100527E-6 7 4.0043334280306854E-5 1.2321025932402108E-5 0.0 9.240769449301581E-6 3.080256483100527E-6 8 4.620384724650791E-5 1.2321025932402108E-5 0.0 9.240769449301581E-6 3.080256483100527E-6 9 7.084589911131213E-5 1.2321025932402108E-5 0.0 1.2321025932402108E-5 3.080256483100527E-6 10 7.022984781469201E-4 1.8481538898603162E-5 0.0 1.8481538898603162E-5 3.080256483100527E-6 11 7.546628383596291E-4 1.8481538898603162E-5 0.0 2.4642051864804215E-5 3.080256483100527E-6 12 7.577430948427297E-4 2.156179538170369E-5 0.0 2.4642051864804215E-5 3.080256483100527E-6 13 7.700641207751317E-4 2.4642051864804215E-5 0.0 2.4642051864804215E-5 3.080256483100527E-6 14 7.916259161568354E-4 2.4642051864804215E-5 0.0 2.4642051864804215E-5 3.080256483100527E-6 15 7.977864291230365E-4 2.4642051864804215E-5 0.0 2.7722308347904742E-5 3.080256483100527E-6 16 8.039469420892375E-4 2.4642051864804215E-5 0.0 3.3882821314105795E-5 3.080256483100527E-6 17 8.224284809878408E-4 2.7722308347904742E-5 3.080256483100527E-6 3.3882821314105795E-5 3.080256483100527E-6 18 8.470705328526449E-4 2.7722308347904742E-5 3.080256483100527E-6 3.3882821314105795E-5 3.080256483100527E-6 19 8.809533541667507E-4 2.7722308347904742E-5 3.080256483100527E-6 4.0043334280306854E-5 3.080256483100527E-6 20 9.887623310752692E-4 3.6963077797206325E-5 3.080256483100527E-6 4.620384724650791E-5 3.080256483100527E-6 21 0.0010318859218386765 3.6963077797206325E-5 6.160512966201054E-6 4.620384724650791E-5 3.080256483100527E-6 22 0.0010657687431527823 3.6963077797206325E-5 6.160512966201054E-6 4.928410372960843E-5 3.080256483100527E-6 23 0.0011674172070950998 4.0043334280306854E-5 6.160512966201054E-6 4.928410372960843E-5 9.240769449301581E-6 24 0.0012413433626895123 4.312359076340738E-5 6.160512966201054E-6 5.236436021270896E-5 9.240769449301581E-6 25 0.0013214300312501261 4.312359076340738E-5 6.160512966201054E-6 5.236436021270896E-5 9.240769449301581E-6 26 0.0014045969562938403 4.620384724650791E-5 6.160512966201054E-6 5.852487317891001E-5 9.240769449301581E-6 27 0.001530887472100962 4.620384724650791E-5 6.160512966201054E-6 6.160512966201054E-5 9.240769449301581E-6 28 0.0018450736333772157 4.928410372960843E-5 6.160512966201054E-6 6.160512966201054E-5 9.240769449301581E-6 29 0.0022239451807985807 5.236436021270896E-5 6.160512966201054E-6 6.160512966201054E-5 9.240769449301581E-6 30 0.0026490205754664533 5.236436021270896E-5 6.160512966201054E-6 6.160512966201054E-5 9.240769449301581E-6 31 0.003536134442599405 6.160512966201054E-5 6.160512966201054E-6 6.776564262821159E-5 9.240769449301581E-6 32 0.005476696026952737 6.160512966201054E-5 6.160512966201054E-6 6.776564262821159E-5 9.240769449301581E-6 33 0.023357584911351297 6.468538614511107E-5 6.160512966201054E-6 6.776564262821159E-5 9.240769449301581E-6 34 0.024278581599798354 6.468538614511107E-5 6.160512966201054E-6 6.776564262821159E-5 1.2321025932402108E-5 35 0.02466361366018592 6.468538614511107E-5 6.160512966201054E-6 6.776564262821159E-5 1.2321025932402108E-5 36 0.02508252854188759 6.776564262821159E-5 6.160512966201054E-6 6.776564262821159E-5 1.5401282415502636E-5 37 0.025479881628207558 7.084589911131213E-5 6.160512966201054E-6 7.084589911131213E-5 1.8481538898603162E-5 38 0.02588647548397683 7.084589911131213E-5 6.160512966201054E-6 7.392615559441265E-5 1.8481538898603162E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCGTATG 2515 0.0 23.955338 13 TATGCCG 2755 0.0 21.632425 16 CGTATGC 2950 0.0 20.643236 14 AAGCAGT 27735 0.0 20.629662 1 CTCGTAT 2990 0.0 19.932281 12 GATCGGA 4020 0.0 16.94139 1 AGCAGTG 34945 0.0 16.417406 2 GTACTTT 24085 0.0 16.410557 22 GAGTACT 24645 0.0 16.293926 20 AGTACTT 24970 0.0 15.932052 21 TAGATCG 1650 0.0 14.8506775 32 TCAACGC 37945 0.0 14.638263 12 GTGGTAT 38380 0.0 14.539754 6 CAACGCA 38150 0.0 14.497117 13 ATCAACG 38555 0.0 14.412305 11 AGTGGTA 38970 0.0 14.347451 5 GGTATCA 39800 0.0 14.053604 8 AACGCAG 40125 0.0 13.767284 14 ATCGGAA 4945 0.0 13.762553 2 TGGTATC 40865 0.0 13.713861 7 >>END_MODULE