FastQCFastQC Report
Thu 26 May 2016
SRR522130_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR522130_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34787377
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1164510.334750734440254No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1117040.3211049801196566No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT630130.181137543080641No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT381670.10971508429623769No Hit
TCTCTGAGCGGGCTGGCAAGGCAGACCGATATCGTATGCCGTCTTCTGCT375470.10793282862344004Illumina PCR Primer Index 1 (95% over 24bp)
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT360480.10362379434356318No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA526400.033.855031
TATGCCG78600.031.21381835
TCGTATG80850.030.04518332
CGTATGC84350.029.0593533
GACCGAT85450.029.01426124
ATATCGT63600.027.46679529
TATCGTA63500.027.2693630
GCGGGCT96300.026.9273438
ACCGATA65700.026.8206125
AGACCGA95150.026.28815323
TGAGCGG101500.025.9610565
GCCGTCT95500.025.62029338
CGATATC68550.025.54613727
CCGTCTT98300.025.1815839
GATATCG70500.025.02673728
AGCGGGC106400.024.7034287
CGGGCTG106500.024.5548349
CAGACCG103450.024.39116322
ATCGTAT72300.024.37698731
ATGCCGT100150.024.27795636
Produced by FastQC (version 0.11.5)