##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR522130_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	34787377
Sequences flagged as poor quality	0
Sequence length	50
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	38.85337716034181	40.0	39.0	40.0	37.0	40.0
2	38.75600859472676	40.0	39.0	40.0	37.0	40.0
3	38.708381088921996	40.0	39.0	40.0	36.0	40.0
4	38.774735761192915	40.0	39.0	40.0	37.0	40.0
5	38.76042450685489	40.0	39.0	40.0	37.0	40.0
6	38.82286592058953	40.0	39.0	40.0	37.0	40.0
7	38.7888110103846	40.0	39.0	40.0	37.0	40.0
8	38.764655696806344	40.0	39.0	40.0	36.0	40.0
9	38.81095024784421	40.0	39.0	40.0	37.0	40.0
10	38.7545691933025	40.0	39.0	40.0	36.0	40.0
11	38.85241589212087	40.0	39.0	40.0	37.0	40.0
12	38.79597424088629	40.0	39.0	40.0	36.0	40.0
13	38.794018847698695	40.0	39.0	40.0	36.0	40.0
14	38.77382146403277	40.0	39.0	40.0	36.0	40.0
15	38.74987246092167	40.0	39.0	40.0	36.0	40.0
16	38.750450084236014	40.0	39.0	40.0	36.0	40.0
17	38.716098974636694	40.0	39.0	40.0	36.0	40.0
18	38.693561891717216	40.0	39.0	40.0	36.0	40.0
19	38.67107525813171	40.0	39.0	40.0	36.0	40.0
20	38.64791866888958	40.0	39.0	40.0	36.0	40.0
21	38.64325844400398	40.0	39.0	40.0	36.0	40.0
22	38.58928257223877	40.0	39.0	40.0	36.0	40.0
23	38.54945390105152	40.0	39.0	40.0	36.0	40.0
24	38.50796623729349	40.0	39.0	40.0	35.0	40.0
25	38.474337056225885	40.0	38.0	40.0	35.0	40.0
26	38.39916317921872	40.0	38.0	40.0	35.0	40.0
27	38.337877472049705	40.0	38.0	40.0	35.0	40.0
28	38.28489515032996	40.0	38.0	40.0	35.0	40.0
29	38.231742220748636	40.0	38.0	40.0	35.0	40.0
30	38.1651403036222	40.0	38.0	40.0	35.0	40.0
31	38.01822678381299	40.0	38.0	40.0	35.0	40.0
32	37.95470371336131	40.0	38.0	40.0	35.0	40.0
33	37.87079813462222	40.0	38.0	40.0	35.0	40.0
34	37.79356782202924	40.0	38.0	40.0	34.0	40.0
35	37.918286107055444	40.0	38.0	40.0	35.0	40.0
36	37.885239206163774	40.0	38.0	40.0	35.0	40.0
37	37.81825534014824	40.0	38.0	40.0	35.0	40.0
38	37.750990395165466	40.0	38.0	40.0	35.0	40.0
39	37.68060891742427	40.0	38.0	40.0	34.0	40.0
40	37.59469985334048	40.0	38.0	40.0	34.0	40.0
41	37.75016032395889	40.0	38.0	40.0	35.0	40.0
42	37.68232022782287	40.0	38.0	40.0	35.0	40.0
43	37.598018959578354	40.0	38.0	40.0	35.0	40.0
44	37.49081125029921	40.0	38.0	40.0	34.0	40.0
45	37.373316763721505	40.0	38.0	40.0	34.0	40.0
46	37.19964600952811	40.0	38.0	40.0	34.0	40.0
47	37.117512970293795	40.0	38.0	40.0	33.0	40.0
48	37.03471934661817	40.0	38.0	40.0	33.0	40.0
49	36.95425349258152	40.0	38.0	40.0	33.0	40.0
50	36.89293179534634	40.0	38.0	40.0	33.0	40.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1	1	-0.012089074978646863
1	2	-0.08462642716862234
1	3	-0.07696997569926367
1	4	-0.06435743853042908
1	5	-0.0665289064087986
1	6	-0.09109262259165973
1	7	-0.11009432621743542
1	8	-0.0975587069302648
1	9	-0.10835260113867662
1	10	-0.1413503548921753
1	11	-0.17503177899727262
1	12	-0.18082167419120765
1	13	-0.17796932892705541
1	14	-0.18065789109000718
1	15	-0.20362760182702289
1	16	-0.1963022016883258
1	17	-0.2133557508504964
1	18	-0.2116808972259605
1	19	-0.22739201950735577
1	20	-0.22521598010425947
1	21	-0.21011440976850793
1	22	-0.258390854441096
1	23	-0.2305523918969712
1	24	-0.247719340404835
1	25	-0.26036033611726594
1	26	-0.25213795439886155
1	27	-0.26340776562422974
1	28	-0.29318138228844504
1	29	-0.2925451008739728
1	30	-0.28994364497696523
1	31	-0.2886843609040568
1	32	-0.30457598359815563
1	33	-0.3001778848029062
1	34	-0.33984218239825736
1	35	-0.2569030243792554
1	36	-0.19218765220471568
1	37	-0.19112002677662332
1	38	-0.19462965633984197
1	39	-0.23377349775316247
1	40	-0.19555663872867513
1	41	-0.223325571066205
1	42	-0.2490704561124133
1	43	-0.25679024924560423
1	44	-0.28593212876474183
1	45	-0.26535885377634116
1	46	-0.26100981671615386
1	47	-0.2992870430038437
1	48	-0.2846885731808655
1	49	-0.3235015571072779
1	50	-0.2677071477039874
2	1	0.039487256916210356
2	2	-0.021068768651666403
2	3	-0.00381779951784722
2	4	0.004747860206464338
2	5	-0.0012045535751141756
2	6	-0.0022555459024573565
2	7	-0.04595870444615713
2	8	-0.036957350447679005
2	9	-0.04103213216388468
2	10	-0.05896948620407727
2	11	-0.10333381845969569
2	12	-0.11827813025121259
2	13	-0.11541391561237901
2	14	-0.11210914277594242
2	15	-0.11825889069913842
2	16	-0.12052928963779408
2	17	-0.15057454030675643
2	18	-0.1469872959111882
2	19	-0.12402796575137387
2	20	-0.1491583440551878
2	21	-0.15672587805289595
2	22	-0.1702886891481441
2	23	-0.17115322035304814
2	24	-0.19321192230270867
2	25	-0.14999238832205464
2	26	-0.16118407856251338
2	27	-0.1743086160195091
2	28	-0.1752261820293839
2	29	-0.19092120391078993
2	30	-0.19744351620743572
2	31	-0.18813127240615302
2	32	-0.1874813269109481
2	33	-0.20815933516959007
2	34	-0.23611145560352043
2	35	-0.14888188167266492
2	36	-0.09567836806233032
2	37	-0.10436794218784229
2	38	-0.11125620579894502
2	39	-0.10270439849701063
2	40	-0.10925897422855257
2	41	-0.13060299630762273
2	42	-0.12934701540069682
2	43	-0.15035416729190842
2	44	-0.12792857258691015
2	45	-0.15714733397301472
2	46	-0.16582275309802696
2	47	-0.20290946432032797
2	48	-0.18781716904389611
2	49	-0.2038235240671682
2	50	-0.23810962992200047
3	1	0.055544178025243696
3	2	-0.02976905164115351
3	3	-0.006362068202520277
3	4	0.0171008323462587
3	5	0.003711301343983564
3	6	-6.573441537298663E-4
3	7	-0.04304438250599674
3	8	-0.03057307574358248
3	9	-0.022381370353230068
3	10	-0.05365643424813271
3	11	-0.06187248192388495
3	12	-0.0844581048532973
3	13	-0.12416736776500414
3	14	-0.09907867020989158
3	15	-0.08856396313155557
3	16	-0.08936281445875238
3	17	-0.11909008984272162
3	18	-0.09813356356283265
3	19	-0.12491785521392273
3	20	-0.10759729019637376
3	21	-0.10666574041501065
3	22	-0.1470871623506227
3	23	-0.11555266624561966
3	24	-0.13459235880712583
3	25	-0.13440341978489556
3	26	-0.12296957067302117
3	27	-0.16653636953794404
3	28	-0.15014211374978714
3	29	-0.16836995893907414
3	30	-0.17249305343837307
3	31	-0.16632767804129855
3	32	-0.1766215212117217
3	33	-0.1931778286208754
3	34	-0.17952037939132737
3	35	-0.10115989488289756
3	36	-0.06873474233518095
3	37	-0.06884856838215825
3	38	-0.07205159751227086
3	39	-0.028721034017436864
3	40	-0.07266124945635966
3	41	-0.09338347051775031
3	42	-0.09716025080915358
3	43	-0.13915256760310513
3	44	-0.09835505987723536
3	45	-0.1365663427439614
3	46	-0.0798398326901335
3	47	-0.10537910514502613
3	48	-0.12326785873439405
3	49	-0.11747856381938249
3	50	-0.11117218580602639
4	1	0.08508999664767458
4	2	0.014115493953660518
4	3	0.016269415368789453
4	4	0.038881273214130374
4	5	0.030691248983274022
4	6	0.021315196897113253
4	7	-0.009084964554517683
4	8	-0.006640024847655468
4	9	-0.004319146079993175
4	10	-0.014875303079740831
4	11	-0.03218227224553516
4	12	-0.04234705113359638
4	13	-0.04282642700736261
4	14	-0.06601827333221877
4	15	-0.07503332374686522
4	16	-0.07192554909151738
4	17	-0.09363793482561533
4	18	-0.07843580611034184
4	19	-0.0758423066124223
4	20	-0.08158756177945747
4	21	-0.0946559974864698
4	22	-0.09163280559150166
4	23	-0.11875338345631548
4	24	-0.08993332797484754
4	25	-0.1045370463756754
4	26	-0.10232132343984546
4	27	-0.13795761389888384
4	28	-0.12300858344198673
4	29	-0.11139829432431725
4	30	-0.135285979538736
4	31	-0.08252571003549036
4	32	-0.1268619315042443
4	33	-0.14934775664541888
4	34	-0.1283809885097682
4	35	-0.0482536862156735
4	36	-0.011350044737774567
4	37	-0.013262581127420958
4	38	-0.025157004166189267
4	39	-0.025362650287256372
4	40	-0.020194381004216666
4	41	-0.026510627156604016
4	42	-0.06338154031492849
4	43	-0.04565447222629615
4	44	-0.13110781975079533
4	45	-0.09524487350471844
4	46	-0.08787425525373038
4	47	-0.08036867422713811
4	48	-0.09846299802570257
4	49	-0.09090198588305043
4	50	-0.10868062204375661
5	1	0.04689674505078045
5	2	-0.024453043330453283
5	3	-0.021103866937430382
5	4	-2.117705231370337E-4
5	5	0.005474907130064821
5	6	-0.035809543234655905
5	7	-0.03142119107904051
5	8	-0.037726465390719
5	9	-0.04072513440414127
5	10	-0.061265824104076216
5	11	-0.07067361873727407
5	12	-0.08312369183413182
5	13	-0.08054665717901344
5	14	-0.10243001642624705
5	15	-0.09551565224602854
5	16	-0.10372156448130454
5	17	-0.12110993094236022
5	18	-0.1349854973355562
5	19	-0.1206852702949135
5	20	-0.10696315601333595
5	21	-0.09351470067714729
5	22	-0.11801502270338915
5	23	-0.13289344800394076
5	24	-0.1160942506451903
5	25	-0.11428261806683793
5	26	-0.13843841715105754
5	27	-0.12689956930140056
5	28	-0.11534975944604042
5	29	-0.14510779950395403
5	30	-0.1715228080211304
5	31	-0.1622021806071885
5	32	-0.16876629157118117
5	33	-0.185835645654727
5	34	-0.1852120357384166
5	35	-0.11499761459128166
5	36	-0.06917405397026499
5	37	-0.07741200417837035
5	38	-0.06774366699295342
5	39	-0.06346438972018831
5	40	-0.04752078561447348
5	41	-0.10591480096823602
5	42	-0.10926899969739168
5	43	-0.11895804467878435
5	44	-0.13952319237441912
5	45	-0.16644059512154286
5	46	-0.15319490706864514
5	47	-0.19646671986854614
5	48	-0.19830068908155596
5	49	-0.17680301082941696
5	50	-0.1593466212445236
6	1	0.06677261419786618
6	2	-0.02104823233122488
6	3	-0.03574227775663985
6	4	-0.02837264975256204
6	5	-0.008837077055233067
6	6	-0.025702886774567446
6	7	-0.01618849418085233
6	8	-0.016398781336896207
6	9	-0.012960716373292769
6	10	-0.03692424171517672
6	11	-0.04457904650760014
6	12	-0.07042144129180627
6	13	-0.05543588340588457
6	14	-0.08510456694247637
6	15	-0.0647873657725242
6	16	-0.0815504161834184
6	17	-0.07703893380207916
6	18	-0.10920939722161904
6	19	-0.10219372001522231
6	20	-0.11375415844961623
6	21	-0.1026429180145314
6	22	-0.10447767928349094
6	23	-0.10687331456401239
6	24	-0.11643697989308066
6	25	-0.10128392462831926
6	26	-0.10078283199393212
6	27	-0.11434209867912415
6	28	-0.08971992250369709
6	29	-0.11106561543548565
6	30	-0.10201620704334857
6	31	-0.08761580429587923
6	32	-0.09236677177841557
6	33	-0.09320977892450344
6	34	-0.10318191501747265
6	35	-0.052582757947213565
6	36	-0.0538144500295914
6	37	-0.04556190704284546
6	38	-0.053025590591730065
6	39	-0.014756946563110773
6	40	-0.06820113761718005
6	41	-0.07237360989670805
6	42	-0.11329127333979017
6	43	-0.0768801946229587
6	44	-0.10068511477182085
6	45	-0.10069541584461916
6	46	-0.09072926271809223
6	47	-0.08820119491570466
6	48	-0.10828726034331027
6	49	-0.0989114725260194
6	50	-0.11114611926707596
7	1	0.044652075251789825
7	2	-0.02897053279691164
7	3	-0.039153788570715164
7	4	-0.012333858144536691
7	5	-0.003523211812357374
7	6	-0.015087156251134104
7	7	-0.014704536994621265
7	8	-0.007979231594724467
7	9	-0.011803449576568426
7	10	-0.037742952214813386
7	11	-0.024757929060115202
7	12	-0.036850247916490275
7	13	-0.03199126715309575
7	14	-0.05123631649527027
7	15	-0.04708405038876151
7	16	-0.07411090564287548
7	17	-0.0524641390923577
7	18	-0.0728812503994547
7	19	-0.07713872057461657
7	20	-0.0990530826721212
7	21	-0.08548097963670642
7	22	-0.0934839040589992
7	23	-0.06962375274387256
7	24	-0.08191775134041279
7	25	-0.0785085468000375
7	26	-0.05226335866760934
7	27	-0.07552942756242942
7	28	-0.0669226607534128
7	29	-0.07988542151301914
7	30	-0.07041621139703125
7	31	-0.0813323437474267
7	32	-0.08704089007417792
7	33	-0.10260093788703983
7	34	-0.09744301372843722
7	35	-0.06271013424227334
7	36	-0.07096014200315892
7	37	-0.06809127481606225
7	38	-0.06527614042114749
7	39	-0.09550841561366497
7	40	-0.05126516192246555
7	41	-0.12570383480979785
7	42	-0.10128702229874165
7	43	-0.16871437700492464
7	44	-0.18216916311528308
7	45	-0.12527812906517966
7	46	-0.15881919276827716
7	47	-0.16252786021635046
7	48	-0.16277774456757044
7	49	-0.21861362824340347
7	50	-0.17380766316560425
8	1	0.06708621064397846
8	2	7.091443022488875E-4
8	3	-0.0022909982470196155
8	4	0.027841155614765967
8	5	0.0162798680819094
8	6	0.03062705529751497
8	7	0.01829117118034418
8	8	0.007537417075340613
8	9	0.011313590444167687
8	10	0.003918992791156484
8	11	-0.015064189934655303
8	12	-0.03270842812631969
8	13	-0.03235923280929853
8	14	-0.03258113112192973
8	15	-0.03891601649483789
8	16	-0.05448431423145905
8	17	-0.06294844418425782
8	18	-0.06514964702031278
8	19	-0.06017407346743653
8	20	-0.0947495617040488
8	21	-0.08075240747549373
8	22	-0.05688665407792115
8	23	-0.07606160448423793
8	24	-0.0742083533133382
8	25	-0.07387985356707816
8	26	-0.0775076114157045
8	27	-0.07375409871321637
8	28	-0.07152327938739944
8	29	-0.08168949659239644
8	30	-0.09085185576076782
8	31	-0.07121519254110353
8	32	-0.07780374181796645
8	33	-0.08733338341902908
8	34	-0.06871521449742346
8	35	-0.034112121769837245
8	36	0.0108211097819364
8	37	0.011845551076696381
8	38	-0.005972510850206447
8	39	0.02780570653474257
8	40	0.012432436680249737
8	41	-0.049838525426928015
8	42	-0.01442637226745802
8	43	-0.06189979995977524
8	44	-0.052320407898676535
8	45	-0.030306939676435718
8	46	-0.08927459309598618
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112	50	-0.1627547878811555
113	1	-0.16082962750097352
113	2	-0.16154498610815438
113	3	-0.1418513008112967
113	4	-0.15346623289252648
113	5	-0.1501722053128134
113	6	-0.1849750680761204
113	7	-0.15066434379727411
113	8	-0.16093888354938457
113	9	-0.15522242594617097
113	10	-0.13597087372340155
113	11	-0.15622715023276612
113	12	-0.1453468181237625
113	13	-0.15053313265969592
113	14	-0.1298823823783266
113	15	-0.16318696172864122
113	16	-0.11610529415881388
113	17	-0.11574418128963515
113	18	-0.11980955420295203
113	19	-0.13257460012224698
113	20	-0.1340487556088803
113	21	-0.1284507765133398
113	22	-0.0971153156518838
113	23	-0.11957549071702545
113	24	-0.10998311198468969
113	25	-0.10776649631609558
113	26	-0.13299898009598365
113	27	-0.13587292821910069
113	28	-0.12825752524614842
113	29	-0.14802429422946517
113	30	-0.1358618473942883
113	31	-0.12223196926240831
113	32	-0.11674963738093425
113	33	-0.14372662660328928
113	34	-0.12737279896951037
113	35	-0.08063017958575358
113	36	-0.0603033232823762
113	37	-0.008454442972258391
113	38	-0.050771268523412516
113	39	-0.08891272341102052
113	40	-0.06814352402638235
113	41	-0.09164761985815773
113	42	-0.08416453029939674
113	43	-0.11144021334875731
113	44	-0.08601193571744403
113	45	-0.07871348168778525
113	46	-0.09378356193192872
113	47	-0.12059926427497913
113	48	-0.09842692920511809
113	49	-0.09718363767164817
113	50	-0.08115369496431413
114	1	-0.1496752667902541
114	2	-0.15732944638931912
114	3	-0.15089380239399475
114	4	-0.1694937808731325
114	5	-0.15493224634514036
114	6	-0.18593564805640028
114	7	-0.1563145156357919
114	8	-0.16291916335067924
114	9	-0.16742745018434846
114	10	-0.1279637092463659
114	11	-0.18124058156785594
114	12	-0.14931046183898644
114	13	-0.1627125837692347
114	14	-0.16546146488099822
114	15	-0.16430560910136194
114	16	-0.13115003007080617
114	17	-0.1281441920531421
114	18	-0.14382968258323103
114	19	-0.15376426720537495
114	20	-0.1364701547181042
114	21	-0.15162394283872516
114	22	-0.12764311882442314
114	23	-0.13611808452891694
114	24	-0.1332060016587917
114	25	-0.13114411332296783
114	26	-0.14092925259445366
114	27	-0.14441795605780072
114	28	-0.12480586384300096
114	29	-0.12391717916295875
114	30	-0.14206619750134308
114	31	-0.13037307150524668
114	32	-0.12036043115151074
114	33	-0.13545444221819025
114	34	-0.1471387513975273
114	35	-0.07360779336043777
114	36	-0.055893220674683164
114	37	-0.05366747258622695
114	38	-0.04762562562341088
114	39	-0.04603833473952079
114	40	-0.04478859608606456
114	41	-0.0919000816910156
114	42	-0.08274351632670829
114	43	-0.10875693041802492
114	44	-0.09539089192546157
114	45	-0.13187146594157184
114	46	-0.18237745427488505
114	47	-0.19745299101961677
114	48	-0.18211076809986082
114	49	-0.190343404336609
114	50	-0.16608829108470502
115	1	-0.18927691672244862
115	2	-0.18184534293711607
115	3	-0.19534241765283156
115	4	-0.19342344594018357
115	5	-0.2008395331533066
115	6	-0.20963710561333926
115	7	-0.19988597875960323
115	8	-0.20358965732187073
115	9	-0.21377304061338975
115	10	-0.1932493702067859
115	11	-0.2150249334095662
115	12	-0.20014473184257753
115	13	-0.2036144130543036
115	14	-0.21692374765721212
115	15	-0.19832702345372866
115	16	-0.17986476023905595
115	17	-0.16128056883093222
115	18	-0.20043974280162047
115	19	-0.1922543038240292
115	20	-0.17759410073112747
115	21	-0.1569648810697899
115	22	-0.1729225838634818
115	23	-0.18630499513982102
115	24	-0.17904226715464233
115	25	-0.17643144247124098
115	26	-0.19567562073240907
115	27	-0.20128312134738024
115	28	-0.20641431391144494
115	29	-0.189866524014505
115	30	-0.21780154445721678
115	31	-0.21569624811577626
115	32	-0.22101740393535607
115	33	-0.2266921638868311
115	34	-0.2375615987424311
115	35	-0.17180310410816446
115	36	-0.1333035016985633
115	37	-0.13953484148239426
115	38	-0.11262083810574808
115	39	-0.09194785250753057
115	40	-0.13624844708695605
115	41	-0.12294315010613843
115	42	-0.1257586172352987
115	43	-0.13648011243172675
115	44	-0.11810283298474644
115	45	-0.1502320667105721
115	46	-0.11785541179629888
115	47	-0.11667486910758385
115	48	-0.13005025358649647
115	49	-0.13307947315390578
115	50	-0.15609684773195198
116	1	-0.15061975023862573
116	2	-0.25293949368985835
116	3	-0.23492267146436774
116	4	-0.24852295047718087
116	5	-0.23817670025086812
116	6	-0.2515598932762515
116	7	-0.25900006897285976
116	8	-0.2706568784825407
116	9	-0.25727055980009794
116	10	-0.2294447202861818
116	11	-0.2606558068577556
116	12	-0.24868596599706194
116	13	-0.2581828505025001
116	14	-0.2647868881522726
116	15	-0.2600782715320804
116	16	-0.22423256393327762
116	17	-0.21854955405384402
116	18	-0.2007150273091085
116	19	-0.23352284376641563
116	20	-0.22816383217614344
116	21	-0.19751108858065436
116	22	-0.21993049685267607
116	23	-0.2146721594184271
116	24	-0.22141561735543291
116	25	-0.2246133354719504
116	26	-0.23976869925883193
116	27	-0.24555568160146635
116	28	-0.25274986599252713
116	29	-0.2458731525934965
116	30	-0.24128784330892472
116	31	-0.26890721618473634
116	32	-0.28410019474883086
116	33	-0.2818850973736886
116	34	-0.30787637210358554
116	35	-0.20530876932785702
116	36	-0.19781183506950129
116	37	-0.22969398760825754
116	38	-0.19479126313390083
116	39	-0.21589994821520264
116	40	-0.20096984755475944
116	41	-0.26181929471565724
116	42	-0.24898478709030059
116	43	-0.2447761517670699
116	44	-0.224950882172962
116	45	-0.2484877644394814
116	46	-0.2277619794346606
116	47	-0.22874841481921493
116	48	-0.2372552470329694
116	49	-0.24897152426461844
116	50	-0.22063409569734205
117	1	-0.2277200325357711
117	2	-0.2130542893425229
117	3	-0.2586808160788223
117	4	-0.25243343974407395
117	5	-0.2371440870840189
117	6	-0.2595440041841499
117	7	-0.24813600740305475
117	8	-0.25108074851671347
117	9	-0.26362763002519074
117	10	-0.24024855116983446
117	11	-0.29428148813261856
117	12	-0.24719041777029815
117	13	-0.27852215421399507
117	14	-0.25171539872201265
117	15	-0.2452847133772238
117	16	-0.20955106061570206
117	17	-0.23063912314825075
117	18	-0.23044014187806994
117	19	-0.23383212161456157
117	20	-0.24641033896875086
117	21	-0.258802608207759
117	22	-0.255693922936878
117	23	-0.2500579212045295
117	24	-0.24999453079624345
117	25	-0.29198663267484903
117	26	-0.2862860727027936
117	27	-0.2944106075189481
117	28	-0.2935039811980218
117	29	-0.30909534839925357
117	30	-0.29395640951054247
117	31	-0.29387382158058273
117	32	-0.2906398777118966
117	33	-0.3169623706710283
117	34	-0.3076123584595791
117	35	-0.2759682808078665
117	36	-0.2226565058121679
117	37	-0.23928844581519826
117	38	-0.24443348527804432
117	39	-0.23231143664865073
117	40	-0.2420348535232506
117	41	-0.2846829441087735
117	42	-0.25142232033707757
117	43	-0.26092446151066895
117	44	-0.2718518871300546
117	45	-0.3215190444153677
117	46	-0.33557055904171307
117	47	-0.3165972671433863
117	48	-0.31800706027270564
117	49	-0.33814860342229025
117	50	-0.3325927137735505
118	1	-0.296743599175322
118	2	-0.26576289387887897
118	3	-0.2998566508727407
118	4	-0.2951766255345376
118	5	-0.250980471788111
118	6	-0.31390303245553497
118	7	-0.28529254450603503
118	8	-0.31072912700912525
118	9	-0.2678449918263013
118	10	-0.2875339270489974
118	11	-0.3057150403771942
118	12	-0.28808133942656156
118	13	-0.3074124677528687
118	14	-0.30561117411220806
118	15	-0.3094726520427784
118	16	-0.2868971467467176
118	17	-0.2649989192650608
118	18	-0.2721783964757165
118	19	-0.29599197954787826
118	20	-0.30104385624669305
118	21	-0.2723570861947522
118	22	-0.2830804811080654
118	23	-0.295785757695171
118	24	-0.2947269442503071
118	25	-0.29874166068283614
118	26	-0.3077392716528351
118	27	-0.3179681659912177
118	28	-0.3437135590560061
118	29	-0.3389562806002431
118	30	-0.3138292539927008
118	31	-0.3586272820015495
118	32	-0.3833496944819714
118	33	-0.37312138507578396
118	34	-0.41181205149898403
118	35	-0.31629570934016016
118	36	-0.24371255379693224
118	37	-0.2557914131635286
118	38	-0.2562606763143549
118	39	-0.27812383503936644
118	40	-0.26971709175132474
118	41	-0.29095104437612207
118	42	-0.31049346608260464
118	43	-0.3353670365796759
118	44	-0.27718982897454936
118	45	-0.2896608992730023
118	46	-0.34592777339064895
118	47	-0.326356816324612
118	48	-0.36693820133989874
118	49	-0.3868076549998918
118	50	-0.3736529502921968
119	1	-0.3232647936178452
119	2	-0.27368077272086566
119	3	-0.30401706235107895
119	4	-0.3078650135661505
119	5	-0.2961111280213089
119	6	-0.32753074698412377
119	7	-0.30974042228444887
119	8	-0.3170943902983083
119	9	-0.3025182491453151
119	10	-0.27786303179794913
119	11	-0.31615447650117545
119	12	-0.30298384168052195
119	13	-0.3015251465663056
119	14	-0.30628426957690635
119	15	-0.2843018442991223
119	16	-0.27142152965127764
119	17	-0.28912359758963646
119	18	-0.28258044350816647
119	19	-0.2709823333125101
119	20	-0.27076083451296284
119	21	-0.2597881746461894
119	22	-0.2816697259675607
119	23	-0.31540908766693576
119	24	-0.30354129319868406
119	25	-0.311677270796487
119	26	-0.30917357652326416
119	27	-0.3372930537748289
119	28	-0.36073275187999343
119	29	-0.342130953359856
119	30	-0.3613560646395868
119	31	-0.36044458160532855
119	32	-0.3872184582476379
119	33	-0.40308008050777744
119	34	-0.38666558923462446
119	35	-0.2840970044428701
119	36	-0.2821759487216582
119	37	-0.2977440411344858
119	38	-0.2775254897942361
119	39	-0.2823174565338036
119	40	-0.3053606457993254
119	41	-0.3361073454416328
119	42	-0.3466118793999655
119	43	-0.4293882286989401
119	44	-0.3666812511328956
119	45	-0.3773387251227618
119	46	-0.3988860521226414
119	47	-0.3609053691427846
119	48	-0.3930057713357016
119	49	-0.4111806071312074
119	50	-0.4358414265824351
120	1	-0.31857940793899076
120	2	-0.27669734910067945
120	3	-0.3018328583266481
120	4	-0.314581496473302
120	5	-0.29114161830852225
120	6	-0.30867775128573527
120	7	-0.3064384619578888
120	8	-0.32240831635099454
120	9	-0.2874818525511742
120	10	-0.28213089880436826
120	11	-0.31617859013682903
120	12	-0.2964072583964992
120	13	-0.32912364628294455
120	14	-0.32258713874704625
120	15	-0.299160483629052
120	16	-0.3060705206395582
120	17	-0.332753953752551
120	18	-0.31357888916194554
120	19	-0.3229280373479426
120	20	-0.3323242473394785
120	21	-0.32133931427854634
120	22	-0.32714556681207085
120	23	-0.3497692545986979
120	24	-0.359967175983094
120	25	-0.3616701784459835
120	26	-0.38131262902484764
120	27	-0.38898888697118394
120	28	-0.3924465376835755
120	29	-0.3997900552388458
120	30	-0.42777125380444403
120	31	-0.42412920281564936
120	32	-0.4193229293196765
120	33	-0.4285637617897251
120	34	-0.41890337277694556
120	35	-0.3180300088912986
120	36	-0.3207411760061376
120	37	-0.30056190461328214
120	38	-0.27567518183771966
120	39	-0.3146970980419894
120	40	-0.3338217996018926
120	41	-0.3406498433128391
120	42	-0.32422717553470193
120	43	-0.30566316357521117
120	44	-0.32603231704337077
120	45	-0.3658149627110845
120	46	-0.34952102750116154
120	47	-0.3526246661336785
120	48	-0.38769129475588926
120	49	-0.3817200713131328
120	50	-0.36316645921027657
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	19893.0
3	1087.0
4	1725.0
5	1620.0
6	2684.0
7	4174.0
8	4611.0
9	5055.0
10	7095.0
11	9175.0
12	10442.0
13	12492.0
14	14650.0
15	16856.0
16	18377.0
17	20402.0
18	23386.0
19	28796.0
20	35181.0
21	35860.0
22	48590.0
23	74584.0
24	104632.0
25	99918.0
26	146803.0
27	183219.0
28	210708.0
29	200744.0
30	241311.0
31	293453.0
32	364072.0
33	420790.0
34	590968.0
35	852818.0
36	1283632.0
37	2289476.0
38	4630500.0
39	2.2090478E7
40	387120.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.88676145242532	20.41263054110998	11.648941110603381	23.051666895861324
2	17.197493792072912	22.820323590364403	37.053733599977946	22.92844901758474
3	18.259174872540694	26.636394574963212	32.165601332920275	22.938829219575823
4	11.858499708098142	16.385078415081423	39.049908821812004	32.70651305500843
5	12.34262990421545	40.52659037512581	35.790928327006746	11.339851393651996
6	30.091300071287353	38.62459362774032	17.91219843910623	13.3719078618661
7	26.912576423670476	33.8061449410351	23.12905727310426	16.152221362190165
8	27.88282342106919	34.95007468107792	21.00832089127804	16.15878100657485
9	27.434831811957512	12.88009913482398	20.406597543499405	39.278471509719104
10	15.689228988530893	28.15773272680212	36.266480020449215	19.886558264217776
11	34.61565383328556	21.898460467427597	24.235977895085334	19.24990780420151
12	19.66694700781838	26.850446355872133	33.635421837064634	19.84718479924485
13	28.81818885051322	21.015395900645224	27.545813528855597	22.62060171998596
14	20.499215002591743	20.646440823820686	30.07326278360288	28.781081389984685
15	22.935798963028805	29.545693334455965	25.15673476331416	22.361772939201067
16	21.62640201358096	27.984018455889903	27.528611887007177	22.86096764352196
17	20.63642510327812	28.382007646049313	29.19267526263909	21.788891988033477
18	20.81956109595619	27.30481885152821	30.123062458059497	21.752557594456103
19	21.63777691341537	27.05906164833477	30.719790402049156	20.583371036200703
20	22.226613981272575	26.34325663587686	30.56809658284958	20.86203280000099
21	22.252583171188792	26.31142612448188	30.197473641085388	21.238517063243947
22	21.80690426875243	26.352791703726325	30.422020608222343	21.418283419298902
23	21.85306620669707	26.510494098497045	30.141410294588034	21.495029400217845
24	21.56211296289078	26.593113877147818	30.30510554661153	21.539667613349877
25	21.713908114155895	26.42801887256488	30.536568577054705	21.321504436224515
26	21.592012072821557	26.474632100701594	30.269305135206515	21.664050691270337
27	21.21492706608759	26.76312644970628	30.229780412973007	21.792166071233126
28	21.464605154065175	26.584161196946777	30.478158620563946	21.473075028424105
29	21.262715406878215	26.87806725269181	30.425694774342865	21.43352256608711
30	21.266975428654145	26.937732072813166	30.292704811647187	21.5025876868855
31	21.5048723527674	26.800598325063596	30.344948343788207	21.3495809783808
32	21.497447677760682	26.660996213170986	30.530809018338463	21.310747090729873
33	21.447270828979725	26.870115020391854	30.305686337945737	21.376927812682688
34	21.852797963070532	26.495885892201247	30.257564546404396	21.39375159832382
35	21.60567461429833	26.618781230226347	30.46478996689672	21.310754188578603
36	21.9006559904349	27.0165031167224	29.75601980245171	21.326821090390986
37	21.819532746484008	26.6400028687489	30.20670664376767	21.333757740999424
38	21.953521860390758	27.042843786656768	29.715734337267207	21.287900015685267
39	21.58350982097893	27.215214441762587	29.785261207945528	21.416014529312953
40	21.599232349976084	26.834178082313898	29.959164602888805	21.607424964821217
41	21.546615833432515	27.091534644139802	29.68228068858244	21.679568833845238
42	21.503753655781964	27.223041248257406	29.90055088279683	21.3726542131638
43	21.656288027417883	26.958401507537356	29.750410310438447	21.634900154606314
44	21.58639102944125	27.340619573355106	29.54564894726629	21.527340449937356
45	21.636343973346193	27.31714002200716	29.576253687472555	21.470262317174097
46	21.70609484485424	27.408913864482532	29.20973203861227	21.675259252050964
47	21.667590791449758	27.44678786743881	29.322975889228793	21.56264545188264
48	21.92795493096104	27.621114558909397	28.72133812980052	21.72959238032904
49	21.825580285144376	27.484171427125297	28.776374244519243	21.913874043211084
50	21.797460688065147	27.561989035569685	28.884387863207074	21.756162413158098
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	19327.0
1	17098.5
2	14870.0
3	34363.5
4	53857.0
5	55766.5
6	57676.0
7	44795.5
8	31915.0
9	41057.0
10	50199.0
11	76217.0
12	102235.0
13	170200.5
14	238166.0
15	235106.5
16	232047.0
17	298562.5
18	365078.0
19	352638.5
20	340199.0
21	383264.5
22	426330.0
23	484431.5
24	542533.0
25	611648.0
26	680763.0
27	779063.5
28	877364.0
29	971464.5
30	1065565.0
31	1185244.0
32	1304923.0
33	1410432.0
34	1515941.0
35	1631006.5
36	1746072.0
37	1840221.5
38	1934371.0
39	1991920.5
40	2049470.0
41	2088472.5
42	2127475.0
43	2158064.0
44	2188653.0
45	2198729.0
46	2208805.0
47	2159584.5
48	2110364.0
49	2067867.5
50	2025371.0
51	1963237.5
52	1901104.0
53	1860853.0
54	1820602.0
55	1683204.5
56	1545807.0
57	1437985.0
58	1330163.0
59	1235794.5
60	1141426.0
61	1013347.5
62	885269.0
63	769985.5
64	654702.0
65	560229.0
66	465756.0
67	377315.0
68	288874.0
69	248037.0
70	207200.0
71	162927.5
72	118655.0
73	91611.5
74	64568.0
75	45908.0
76	27248.0
77	20550.5
78	13853.0
79	10065.5
80	6278.0
81	4727.5
82	3177.0
83	2381.0
84	1585.0
85	1193.5
86	802.0
87	665.0
88	528.0
89	304.0
90	80.0
91	57.5
92	35.0
93	25.5
94	16.0
95	17.0
96	18.0
97	22.0
98	26.0
99	31.0
100	36.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.31038844923547987
2	0.0
3	0.0
4	0.0
5	9.773660141148326E-5
6	0.0
7	0.00532377017100197
8	3.3920349901632425E-4
9	1.4373029619335773E-5
10	0.0020380956000218124
11	0.0
12	0.0
13	0.0
14	2.8746059238671546E-6
15	2.9895901608218404E-4
16	0.0
17	0.0
18	2.8746059238671546E-6
19	4.0244482934140164E-5
20	0.0
21	0.0
22	0.0
23	0.0020984623244230225
24	0.001900114515676189
25	0.006554101506417112
26	0.001379810843456234
27	0.01160765872057557
28	0.015175044672094707
29	0.01738561662754855
30	0.012596523158385872
31	0.019984260382724457
32	0.020380956000218126
33	0.022436299235783143
34	0.02516717486345694
35	0.03614242028078173
36	0.041132736164615116
37	0.03632352045398536
38	0.02737199760706304
39	0.02783480916080566
40	0.034940835004605264
41	0.03213234501698706
42	0.03740149767543555
43	0.030643299148423866
44	0.020191232009242895
45	0.03020635904799606
46	0.03630052360659443
47	0.03487184446243245
48	0.03786430922917816
49	0.04259303597393963
50	0.031925373390468616
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	3.4787377E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.666006769539226
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.14282340675892	20.590022771176457
2	13.679935753771124	8.39018004864014
3	6.010102554964264	5.529175369084774
4	3.342646664542668	4.1002250097217265
5	2.1006232757127514	3.2208863796629448
6	1.4011610912984256	2.5780809306583494
7	1.0669564992058256	2.2903506659214807
8	0.7484758349911279	1.8362212018139563
9	0.5665726664251411	1.5637069101626342
>10	3.38741309388347	19.343573551069902
>50	0.3034220571483036	6.479529716572518
>100	0.220531551966775	13.229890530731192
>500	0.019811615057589452	4.173620376469456
>1k	0.009166007790486194	4.788563775827237
>5k	2.637353042964615E-4	0.5228963580975194
>10k+	9.419117931913487E-5	1.36307640438982
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	116451	0.334750734440254	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	111704	0.3211049801196566	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	63013	0.181137543080641	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	38167	0.10971508429623769	No Hit
TCTCTGAGCGGGCTGGCAAGGCAGACCGATATCGTATGCCGTCTTCTGCT	37547	0.10793282862344004	Illumina PCR Primer Index 1 (95% over 24bp)
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	36048	0.10362379434356318	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	2.8746059238671546E-6	0.0
4	0.0	0.0	0.0	8.623817771601463E-6	0.0
5	0.0	0.0	0.0	8.623817771601463E-6	0.0
6	0.0	0.0	0.0	1.1498423695468618E-5	0.0
7	0.0	0.0	0.0	1.1498423695468618E-5	0.0
8	0.0	2.8746059238671546E-6	0.0	1.4373029619335772E-5	0.0
9	0.0	2.8746059238671546E-6	0.0	2.0122241467070082E-5	0.0
10	0.0	2.8746059238671546E-6	0.0	2.2996847390937237E-5	0.0
11	0.0	2.8746059238671546E-6	0.0	2.8746059238671543E-5	0.0
12	0.0	2.8746059238671546E-6	0.0	3.16206651625387E-5	0.0
13	0.0	5.749211847734309E-6	0.0	3.7369877010273005E-5	0.0
14	0.0	5.749211847734309E-6	0.0	4.0244482934140164E-5	0.0
15	0.0	5.749211847734309E-6	0.0	5.174290662960878E-5	0.0
16	0.0	5.749211847734309E-6	0.0	5.4617512553475935E-5	0.0
17	0.0	5.749211847734309E-6	0.0	5.749211847734309E-5	0.0
18	0.0	8.623817771601463E-6	0.0	5.749211847734309E-5	0.0
19	0.0	1.1498423695468618E-5	0.0	6.0366724401210245E-5	0.0
20	0.0	1.1498423695468618E-5	0.0	6.32413303250774E-5	0.0
21	0.0	1.1498423695468618E-5	0.0	7.473975402054601E-5	0.0
22	0.0	1.1498423695468618E-5	0.0	1.0348581325921755E-4	0.0
23	0.0	1.1498423695468618E-5	0.0	1.1210963103081902E-4	0.0
24	0.0	1.1498423695468618E-5	0.0	1.2935726657402195E-4	0.0
25	2.8746059238671546E-6	1.4373029619335772E-5	0.0	1.4085569026949058E-4	0.0
26	2.8746059238671546E-6	1.4373029619335772E-5	0.0	1.5235411396495919E-4	0.0
27	2.8746059238671546E-6	1.4373029619335772E-5	0.0	1.8684938505136503E-4	0.0
28	2.8746059238671546E-6	1.4373029619335772E-5	0.0	2.328430798332395E-4	0.0
29	2.8746059238671546E-6	1.4373029619335772E-5	0.0	3.018336220060512E-4	0.0
30	2.8746059238671546E-6	1.4373029619335772E-5	0.0	4.0819404118913594E-4	0.0
31	5.749211847734309E-6	2.0122241467070082E-5	0.0	5.57673549230228E-4	0.0
32	5.749211847734309E-6	2.0122241467070082E-5	0.0	7.301499046622572E-4	0.0
33	5.749211847734309E-6	2.0122241467070082E-5	0.0	0.0010837264332979172	0.0
34	5.749211847734309E-6	2.2996847390937237E-5	0.0	0.0017506350076350972	2.8746059238671546E-6
35	5.749211847734309E-6	2.2996847390937237E-5	0.0	0.0028544836824000845	2.8746059238671546E-6
36	8.623817771601463E-6	2.2996847390937237E-5	0.0	0.004717228321066001	2.8746059238671546E-6
37	8.623817771601463E-6	3.4495271086405854E-5	0.0	0.00787929483731987	2.8746059238671546E-6
38	8.623817771601463E-6	3.7369877010273005E-5	0.0	0.012225698994207009	2.8746059238671546E-6
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	52640	0.0	33.85503	1
TATGCCG	7860	0.0	31.213818	35
TCGTATG	8085	0.0	30.045183	32
CGTATGC	8435	0.0	29.05935	33
GACCGAT	8545	0.0	29.014261	24
ATATCGT	6360	0.0	27.466795	29
TATCGTA	6350	0.0	27.26936	30
GCGGGCT	9630	0.0	26.927343	8
ACCGATA	6570	0.0	26.82061	25
AGACCGA	9515	0.0	26.288153	23
TGAGCGG	10150	0.0	25.961056	5
GCCGTCT	9550	0.0	25.620293	38
CGATATC	6855	0.0	25.546137	27
CCGTCTT	9830	0.0	25.18158	39
GATATCG	7050	0.0	25.026737	28
AGCGGGC	10640	0.0	24.703428	7
CGGGCTG	10650	0.0	24.554834	9
CAGACCG	10345	0.0	24.391163	22
ATCGTAT	7230	0.0	24.376987	31
ATGCCGT	10015	0.0	24.277956	36
>>END_MODULE