SRR522129_1.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	SRR522129_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	28390312
Sequences flagged as poor quality	0
Sequence length	50
%GC	43

Per base quality graph

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
TCTCTGAGCGGGCTGGCAAGGCAGACCGATATCGTATGCCGTCTTCTGCT	218616	0.7700373282266147	Illumina PCR Primer Index 1 (95% over 24bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	176929	0.6232020275085388	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	167849	0.5912192863537393	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	98043	0.3453396355770941	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	66021	0.23254763808161039	No Hit
TCTCTGAGCGGGCTGGCAAGGCAGACCGATCTCGTATGCCGTCTTCTGCT	65214	0.22970511912655273	Illumina Paired End PCR Primer 2 (96% over 29bp)
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	61205	0.21558410488761096	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	53655	0.18899052606396155	No Hit

Adapter graph

Kmer graph

Sequence	Count	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	62980	36.277534	1
TATGCCG	38250	35.482666	35
CCGTCTT	38880	34.884155	39
GACCGAT	38685	34.745747	24
TGAGCGG	40750	34.736366	5
GCCGTCT	39140	34.646927	38
TCGTATG	39010	34.537434	32
GCGGGCT	40995	34.436535	8
CGTATGC	39635	34.253567	33
AGACCGA	40170	34.11929	23
ATGCCGT	39730	34.100082	36
ATATCGT	29460	34.072113	29
TATCGTA	29475	34.06976	30
CGTCTTC	40020	33.90157	40
TGCCGTC	40075	33.844418	37
AGCGGGC	41805	33.822926	7
ACCGATA	29900	33.817757	25
CGGGCTG	41805	33.81115	9
CGATATC	29885	33.7325	27
ATCGTAT	30120	33.64889	31