SRR522128_1.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	SRR522128_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	27545351
Sequences flagged as poor quality	0
Sequence length	50
%GC	43

Per base quality graph

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	260612	0.9461197281530375	No Hit
TCTCTGAGCGGGCTGGCAAGGCAGACCGATATCGTATGCCGTCTTCTGCT	259360	0.9415744965457147	Illumina PCR Primer Index 1 (95% over 24bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	257440	0.9346041733140376	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	146513	0.5318973789805764	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	132203	0.47994668864448303	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	102299	0.37138390431111223	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	95115	0.3453032782192538	No Hit
TCTCTGAGCGGGCTGGCAAGGCAGACCGATCTCGTATGCCGTCTTCTGCT	77403	0.2810020464070325	Illumina Paired End PCR Primer 2 (96% over 29bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	53741	0.1951000733299786	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	34414	0.12493578317444567	No Hit

Adapter graph

Kmer graph

Sequence	Count	Obs/Exp Max	Max Obs/Exp Position
TATGCCG	44960	36.834953	35
CCGTCTT	45630	36.15404	39
GCCGTCT	45740	36.047855	38
TGAGCGG	47925	35.944717	5
TCGTATG	45685	35.881916	32
CGTATGC	46270	35.77065	33
ATGCCGT	46180	35.747536	36
GACCGAT	45765	35.740902	24
TATCGTA	34030	35.70403	30
GCGGGCT	48260	35.690323	8
CGTCTTC	46320	35.662987	40
ATATCGT	34235	35.52718	29
TGCCGTC	46500	35.47787	37
GATATCG	34570	35.3408	28
CGGGCTG	48805	35.317463	9
AGACCGA	47530	35.20161	23
ATCGTAT	34910	35.17671	31
AGCGGGC	49105	35.11679	7
CGATATC	34730	35.11565	27
ACCGATA	35095	34.831875	25