FastQCFastQC Report
Thu 26 May 2016
SRR522127_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR522127_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25936670
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCG2172470.8376055985598768Illumina Paired End PCR Primer 2 (100% over 31bp)
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT429560.16561879377730448No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCCGA284250.035.26259614
GATCGGA286750.034.88043621
CCGAGAT288950.034.72220217
CGAGATC290350.034.5250218
AGATCGG291400.034.39990220
TGCCGAG294100.034.14263515
GAGATCG296600.033.79776419
ATCGGAA296100.033.72153522
GCCGAGA298600.033.63740516
TCGGAAG300950.033.14039223
AAGAGCG302350.032.82124327
AGAGCGG304350.032.6285728
GAGCGGT306450.032.35547629
AGCGGTT312900.031.69786830
GCGGTTC313200.031.647131
CGGAAGA334550.029.83159624
GAAGAGC413250.024.34237726
GGAAGAG467550.021.67973925
AATGCCG502000.019.97567713
CGGTTCA526500.019.1897931
Produced by FastQC (version 0.11.5)