##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR522127_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	25936670
Sequences flagged as poor quality	0
Sequence length	50
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	39.35612941059897	40.0	39.0	40.0	38.0	40.0
2	39.28368082718406	40.0	39.0	40.0	38.0	40.0
3	39.27569294747553	40.0	39.0	40.0	38.0	40.0
4	39.276254044948715	40.0	39.0	40.0	38.0	40.0
5	39.2728323643706	40.0	39.0	40.0	38.0	40.0
6	39.30664187037118	40.0	39.0	40.0	38.0	40.0
7	39.27954826120701	40.0	39.0	40.0	38.0	40.0
8	39.26842320159064	40.0	39.0	40.0	38.0	40.0
9	39.25887725756622	40.0	39.0	40.0	38.0	40.0
10	39.242687785286236	40.0	39.0	40.0	38.0	40.0
11	39.253111405589074	40.0	39.0	40.0	38.0	40.0
12	39.22747368879659	40.0	39.0	40.0	38.0	40.0
13	39.20060759534667	40.0	39.0	40.0	38.0	40.0
14	39.18650778993602	40.0	39.0	40.0	38.0	40.0
15	39.17109752331352	40.0	39.0	40.0	38.0	40.0
16	39.153375163426915	40.0	39.0	40.0	38.0	40.0
17	39.127087247514815	40.0	39.0	40.0	38.0	40.0
18	39.101071378862436	40.0	39.0	40.0	38.0	40.0
19	39.07502589962397	40.0	39.0	40.0	38.0	40.0
20	39.033556389467115	40.0	39.0	40.0	38.0	40.0
21	39.00519658074842	40.0	39.0	40.0	38.0	40.0
22	38.953483118688716	40.0	39.0	40.0	38.0	40.0
23	38.9254979532839	40.0	39.0	40.0	37.0	40.0
24	38.893245123602995	40.0	39.0	40.0	37.0	40.0
25	38.85562745718706	40.0	39.0	40.0	37.0	40.0
26	38.74200535380987	40.0	39.0	40.0	36.0	40.0
27	38.66055156656579	40.0	39.0	40.0	36.0	40.0
28	38.610687339585226	40.0	39.0	40.0	36.0	40.0
29	38.56680965598128	40.0	39.0	40.0	36.0	40.0
30	38.482697894525394	40.0	39.0	40.0	36.0	40.0
31	38.42496338196075	40.0	39.0	40.0	36.0	40.0
32	38.32472302728145	40.0	38.0	40.0	35.0	40.0
33	38.55919094471264	40.0	39.0	40.0	36.0	40.0
34	38.52466168555948	40.0	39.0	40.0	36.0	40.0
35	38.46396210461867	40.0	39.0	40.0	36.0	40.0
36	38.53046539898915	40.0	39.0	40.0	36.0	40.0
37	38.473960188412775	40.0	39.0	40.0	36.0	40.0
38	38.41946105648875	40.0	39.0	40.0	36.0	40.0
39	38.397719483649986	40.0	39.0	40.0	36.0	40.0
40	38.341157673672065	40.0	39.0	40.0	36.0	40.0
41	38.27693875119667	40.0	39.0	40.0	36.0	40.0
42	38.212944568443056	40.0	39.0	40.0	35.0	40.0
43	38.15920524878483	40.0	39.0	40.0	35.0	40.0
44	38.10164315619546	40.0	39.0	40.0	35.0	40.0
45	38.04974566896984	40.0	39.0	40.0	35.0	40.0
46	37.948727882183796	40.0	38.0	40.0	35.0	40.0
47	37.894192006915304	40.0	38.0	40.0	35.0	40.0
48	37.91498087456871	40.0	38.0	40.0	35.0	40.0
49	37.85779546873211	40.0	38.0	40.0	35.0	40.0
50	37.80959132378983	40.0	38.0	40.0	35.0	40.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1	1	-0.08970602161507202
1	2	-0.0921247905780831
1	3	-0.09316109218212887
1	4	-0.08711145153081645
1	5	-0.08215279266924824
1	6	-0.09949822175858714
1	7	-0.1039209791380884
1	8	-0.10275526982854188
1	9	-0.11444346510803172
1	10	-0.10124230037651216
1	11	-0.12579742069046063
1	12	-0.12411166504222138
1	13	-0.12399233095842987
1	14	-0.12635773900262137
1	15	-0.14718503701519836
1	16	-0.15769793630088458
1	17	-0.17469662138056918
1	18	-0.19474274437845196
1	19	-0.16054978772591966
1	20	-0.17684645911507602
1	21	-0.15966158329663216
1	22	-0.17860790389851644
1	23	-0.15707430251320886
1	24	-0.174148320359933
1	25	-0.1829218471480374
1	26	-0.1958651049893234
1	27	-0.20952175540268314
1	28	-0.21714183882401983
1	29	-0.221040466424256
1	30	-0.2477239984705193
1	31	-0.22401808610159435
1	32	-0.21262125797752418
1	33	-0.18739055984110564
1	34	-0.18611146409779877
1	35	-0.200953323165038
1	36	-0.2112686395612755
1	37	-0.22258266820550432
1	38	-0.1997045736185683
1	39	-0.20934868559975683
1	40	-0.23270729873973295
1	41	-0.23400502944176793
1	42	-0.22782317904913896
1	43	-0.24629795081757067
1	44	-0.2561881821588301
1	45	-0.2870485385952293
1	46	-0.24578752540787718
1	47	-0.23513164234727668
1	48	-0.21168058803957024
1	49	-0.17265585720661392
1	50	-0.20218198717558522
2	1	-0.091221711174029
2	2	-0.07616354942669545
2	3	-0.08289090227106044
2	4	-0.07379245120562672
2	5	-0.06952002185504824
2	6	-0.08750714378492574
2	7	-0.09792992279958668
2	8	-0.09016911477676359
2	9	-0.1114024670395466
2	10	-0.10542771730275291
2	11	-0.10570773760858998
2	12	-0.1000437449754159
2	13	-0.12070860677596329
2	14	-0.11963571487375901
2	15	-0.11955446052507313
2	16	-0.13290989122344854
2	17	-0.12292340509280564
2	18	-0.12941591641053662
2	19	-0.11898811835467171
2	20	-0.15005074988715705
2	21	-0.13240139074348178
2	22	-0.1503223047530966
2	23	-0.1599803344353603
2	24	-0.18682979705404534
2	25	-0.17543476757067822
2	26	-0.19968908437440547
2	27	-0.1938594971292602
2	28	-0.19945320772237807
2	29	-0.2257685436845236
2	30	-0.21236117020583833
2	31	-0.21141392485316146
2	32	-0.1984699845299076
2	33	-0.18847519692891979
2	34	-0.20139696570230825
2	35	-0.20873738213171578
2	36	-0.1915490238273705
2	37	-0.22748820856730845
2	38	-0.2218212532562518
2	39	-0.24018700176054608
2	40	-0.2465583229533621
2	41	-0.22915479765199365
2	42	-0.2165435332765142
2	43	-0.24485720937455824
2	44	-0.25000453102431663
2	45	-0.25937533441912564
2	46	-0.26519609165494984
2	47	-0.28376453194693596
2	48	-0.22021953695499263
2	49	-0.2390252757770739
2	50	-0.2561185207254226
3	1	-0.049894461205518326
3	2	-0.04954405124748007
3	3	-0.03868242904439256
3	4	-0.04010450459698944
3	5	-0.0497776350759338
3	6	-0.05701349680312262
3	7	-0.07441640162890195
3	8	-0.05645871527971025
3	9	-0.06658768391003633
3	10	-0.05663458719980241
3	11	-0.08998178370966059
3	12	-0.08368097646967243
3	13	-0.05663710927512966
3	14	-0.09727380088843063
3	15	-0.09368256209651094
3	16	-0.06427091019800457
3	17	-0.08846292107376996
3	18	-0.09758762759479112
3	19	-0.11459750814521641
3	20	-0.09376884024135279
3	21	-0.08538119878977568
3	22	-0.08630309988323148
3	23	-0.08762351712238825
3	24	-0.10092605786302045
3	25	-0.0893420572638206
3	26	-0.13107843970045252
3	27	-0.12556921770069351
3	28	-0.16718462449467353
3	29	-0.16020830837278766
3	30	-0.17650149169634233
3	31	-0.18244878487299587
3	32	-0.15391027032467264
3	33	-0.1510952431262922
3	34	-0.1569513742975559
3	35	-0.16817207034543458
3	36	-0.17971545055994653
3	37	-0.1666993092188065
3	38	-0.16435570744963712
3	39	-0.19135064595239015
3	40	-0.19056628991084779
3	41	-0.1703254673540684
3	42	-0.1842772854447432
3	43	-0.18526162667606627
3	44	-0.181142522899556
3	45	-0.18108739204086532
3	46	-0.18510197911790982
3	47	-0.17846774683611955
3	48	-0.16057866460096193
3	49	-0.13473425285738472
3	50	-0.15354852399033803
4	1	-0.05075890660016569
4	2	-0.0616105156354152
4	3	-0.06667080233221867
4	4	-0.055505047985739964
4	5	-0.047690177828499714
4	6	-0.06498970030355622
4	7	-0.07087021356136347
4	8	-0.0767242758312463
4	9	-0.06770252726452242
4	10	-0.07118265641111066
4	11	-0.06065876221813937
4	12	-0.08285315729909115
4	13	-0.0940284936376159
4	14	-0.08711057001652023
4	15	-0.06646253193189722
4	16	-0.08975397375219529
4	17	-0.08279944272545237
4	18	-0.09034304598384324
4	19	-0.11851675164410125
4	20	-0.10684668246365447
4	21	-0.08587429892696008
4	22	-0.09717268007496926
4	23	-0.08733749053991602
4	24	-0.0988344201905278
4	25	-0.06969201679682868
4	26	-0.12137510308944144
4	27	-0.10707245199579773
4	28	-0.11477178528821241
4	29	-0.10104826312728932
4	30	-0.12252545854823182
4	31	-0.16163739382277953
4	32	-0.15451334414370876
4	33	-0.15719263059340705
4	34	-0.19011041082548985
4	35	-0.2073484962064711
4	36	-0.16012607376109145
4	37	-0.17274301622782673
4	38	-0.19897969793043302
4	39	-0.2009545074490191
4	40	-0.18206159517474418
4	41	-0.22460092344675786
4	42	-0.19591145587811098
4	43	-0.18587078111336552
4	44	-0.18809927274448768
4	45	-0.1919303695277108
4	46	-0.1826076905048808
4	47	-0.18774444256734313
4	48	-0.15158994315023477
4	49	-0.15381518545891026
4	50	-0.19340080107023994
5	1	-0.03783475366573441
5	2	-0.052557368454436926
5	3	-0.017624431070849766
5	4	-0.03725120875660082
5	5	-0.03472671629885582
5	6	-0.04076955971815721
5	7	-0.04936757949760562
5	8	-0.05033062951896028
5	9	-0.047784562444235235
5	10	-0.04902033129525307
5	11	-0.06318637562624474
5	12	-0.0596802282534199
5	13	-0.056143961646739626
5	14	-0.057510777410819
5	15	-0.07919133639644116
5	16	-0.07521264298901542
5	17	-0.050402251822902144
5	18	-0.06988514102242505
5	19	-0.06718258744275118
5	20	-0.05632205477375152
5	21	-0.060560961651233924
5	22	-0.07447658254044143
5	23	-0.07247535813043982
5	24	-0.06650270357401666
5	25	-0.06893207731565099
5	26	-0.09146285036091228
5	27	-0.09718785345291536
5	28	-0.07437559221872192
5	29	-0.1093176340896207
5	30	-0.12759908342861337
5	31	-0.09371102520454144
5	32	-0.10420835210343427
5	33	-0.09610151818286994
5	34	-0.11245549354887174
5	35	-0.09873187246008541
5	36	-0.10119652368413057
5	37	-0.10635811443469123
5	38	-0.12570459264617995
5	39	-0.1192544851459516
5	40	-0.1215307745543015
5	41	-0.10261270911519915
5	42	-0.13681089956505588
5	43	-0.13019677416351527
5	44	-0.11811542064933889
5	45	-0.1558193321788366
5	46	-0.09816803161840681
5	47	-0.07751613471957342
5	48	-0.06419488416591435
5	49	-0.0603847489624485
5	50	-0.07312612918318706
6	1	-0.03556481566767644
6	2	-0.02570046057147124
6	3	-0.018514526960736077
6	4	-0.030012579566694342
6	5	-0.017691111951833705
6	6	-0.03709196776689794
6	7	-0.035539038372263576
6	8	-0.05048612724976209
6	9	-0.06498117507091195
6	10	-0.03723436129689617
6	11	-0.05266447879318292
6	12	-0.04443741001676216
6	13	-0.04419324757562748
6	14	-0.044121954036043576
6	15	-0.061600733075103165
6	16	-0.06294364476476488
6	17	-0.060718625844373264
6	18	-0.05984065348438605
6	19	-0.06055140396893677
6	20	-0.057429463407942194
6	21	-0.07244387598861834
6	22	-0.0673571227796117
6	23	-0.08918722365996246
6	24	-0.08949566507224915
6	25	-0.07611071535840352
6	26	-0.07372620733436719
6	27	-0.1030424034746531
6	28	-0.10001779495112118
6	29	-0.08623068335615613
6	30	-0.0815823172772312
6	31	-0.09612794617016363
6	32	-0.05310224015862275
6	33	-0.0792589320707151
6	34	-0.061909029151387074
6	35	-0.1067271674064898
6	36	-0.09425257676682719
6	37	-0.09365548140131352
6	38	-0.08209288809547388
6	39	-0.12137539446563039
6	40	-0.10347587277665582
6	41	-0.11710221127748355
6	42	-0.11762521589102448
6	43	-0.09948872760352856
6	44	-0.1295851860747561
6	45	-0.11294413094007894
6	46	-0.1169444478119317
6	47	-0.13456494175907352
6	48	-0.07923218297791834
6	49	-0.10572268458966505
6	50	-0.10285666297210838
7	1	-0.043382297669282366
7	2	-0.045060063698102226
7	3	-0.041123198342972955
7	4	-0.04014341860734305
7	5	-0.03877890914313298
7	6	-0.05916089424150783
7	7	-0.035981481941149696
7	8	-0.049896782525621575
7	9	-0.04799353881989532
7	10	-0.05017060076199442
7	11	-0.05393714756517909
7	12	-0.07608522583004884
7	13	-0.06977756061624518
7	14	-0.07471270191606294
7	15	-0.08104773339628935
7	16	-0.08114617905231114
7	17	-0.08783023357494812
7	18	-0.11375209130111585
7	19	-0.10461162631023058
7	20	-0.09401177489667845
7	21	-0.0840267588570569
7	22	-0.08207839509238823
7	23	-0.08383046754835277
7	24	-0.09083867046265226
7	25	-0.07720683932137007
7	26	-0.10021943194028182
7	27	-0.07715802581568454
7	28	-0.11232524850122161
7	29	-0.11358723295260376
7	30	-0.13087258458547524
7	31	-0.11716414896035587
7	32	-0.1233396554939219
7	33	-0.1094158887522525
7	34	-0.0932650957007155
7	35	-0.09340800896684698
7	36	-0.12036714324355557
7	37	-0.14195431292620242
7	38	-0.12028024180602159
7	39	-0.13391618002369654
7	40	-0.1398961517787356
7	41	-0.11536591507265115
7	42	-0.12111399882528673
7	43	-0.13008310356414654
7	44	-0.11393724351592738
7	45	-0.08720447758801697
7	46	-0.1273697081721039
7	47	-0.09730645921550973
7	48	-0.06143922975862637
7	49	-0.04973139368880908
7	50	-0.05199585631656589
8	1	-0.03756126025148632
8	2	-0.05532727466330556
8	3	-0.04223745527693268
8	4	-0.06859692873483425
8	5	-0.0453110023180443
8	6	-0.046594404356227415
8	7	-0.06369680084039686
8	8	-0.04962585346881099
8	9	-0.05272585857066048
8	10	-0.05060359775027479
8	11	-0.0366611104421537
8	12	-0.0531048870218811
8	13	-0.034749711109775205
8	14	-0.04578565121401823
8	15	-0.06628071642310829
8	16	-0.0641662910121994
8	17	-0.06213911440809028
8	18	-0.06611167481526792
8	19	-0.06937839853210903
8	20	-0.06924496436590033
8	21	-0.07487433689388467
8	22	-0.061851508501419517
8	23	-0.07390707896436055
8	24	-0.05973503573546424
8	25	-0.07836568355887152
8	26	-0.057320273948626266
8	27	-0.10967185373252875
8	28	-0.09007437500508786
8	29	-0.06320283506104118
8	30	-0.07439590097847315
8	31	-0.07029523116337799
8	32	-0.06463083995617325
8	33	-0.06342765001033257
8	34	-0.08808711574180705
8	35	-0.0752369269511064
8	36	-0.07160746838396648
8	37	-0.056898444715628216
8	38	-0.08180267581734313
8	39	-0.08152013920598478
8	40	-0.07249408901470389
8	41	-0.08156671713332742
8	42	-0.09682221865963925
8	43	-0.08394939741426555
8	44	-0.11496477335590072
8	45	-0.09176398163247512
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113	11	-0.06139583530142545
113	12	-0.05538784114184381
113	13	-0.0579224435486978
113	14	-0.05832828997908024
113	15	-0.06655943267883657
113	16	-0.0498332586647976
113	17	-0.0674173036079111
113	18	-0.034190413152551
113	19	-0.043314685448144985
113	20	-0.036126409154199735
113	21	-0.04410959514208912
113	22	-0.044501208114454016
113	23	-0.049163178838703914
113	24	-0.06186615134610918
113	25	-0.053805754197675526
113	26	-0.04447911732721366
113	27	-0.01295716412318626
113	28	-0.0538208715189441
113	29	-0.06588089031976807
113	30	-0.07497079763925285
113	31	-0.07224697733207108
113	32	-0.08025329497264977
113	33	-0.08606752055509048
113	34	-0.07540171619740477
113	35	-0.08867046491661057
113	36	-0.08417729242634664
113	37	-0.11443439951645473
113	38	-0.09075279909325928
113	39	-0.0862097253138927
113	40	-0.11080359823785102
113	41	-0.09120834782682863
113	42	-0.08489065058207501
113	43	-0.06148466460057023
113	44	-0.09814851043341122
113	45	-0.0586067438262603
113	46	-0.07187733156882103
113	47	-0.08373558992752095
113	48	-0.07164626531576346
113	49	-0.06994143039523948
113	50	-0.06991015951504664
114	1	-0.07450659841267537
114	2	-0.0771745467299354
114	3	-0.07746497747139358
114	4	-0.05579342209856719
114	5	-0.08791762592164076
114	6	-0.09738340708685911
114	7	-0.0950148916422009
114	8	-0.09478199040977842
114	9	-0.09830582980035985
114	10	-0.08758294854101223
114	11	-0.07856318696171627
114	12	-0.10326302029582024
114	13	-0.10859284373554345
114	14	-0.08499762637194408
114	15	-0.08705301699100687
114	16	-0.0884116842404552
114	17	-0.07834247387712168
114	18	-0.08738954077858097
114	19	-0.0957426509747279
114	20	-0.10012773287746768
114	21	-0.1240753872847904
114	22	-0.08126403355348799
114	23	-0.09879346873835715
114	24	-0.11429466527459908
114	25	-0.10027649258859839
114	26	-0.11063277317187215
114	27	-0.1321999434573442
114	28	-0.13064956359480107
114	29	-0.11113213483860562
114	30	-0.11308451536849162
114	31	-0.06549900750601978
114	32	-0.09566624616685004
114	33	-0.12354890445774203
114	34	-0.11855258471999974
114	35	-0.1042108138379163
114	36	-0.09053029064594398
114	37	-0.0878068771171101
114	38	-0.11559557061500669
114	39	-0.09684481079660401
114	40	-0.09029712978451698
114	41	-0.10213233209432815
114	42	-0.0954957193194943
114	43	-0.0797484864065865
114	44	-0.06842353808575297
114	45	-0.1235824555022802
114	46	-0.08025198698577185
114	47	-0.05706705631576625
114	48	-0.05223743979534845
114	49	-0.06964774135283136
114	50	-0.01761869320503706
115	1	-0.05770903529926841
115	2	-0.07048512698792564
115	3	-0.0554184596975702
115	4	-0.05739486856634102
115	5	-0.04893817255153721
115	6	-0.08116615003817174
115	7	-0.06301537159800574
115	8	-0.07232445688368472
115	9	-0.07541915549909817
115	10	-0.06970149606534193
115	11	-0.09006806148848767
115	12	-0.07283749932809513
115	13	-0.07384544678766503
115	14	-0.07159782292774253
115	15	-0.08796240590878313
115	16	-0.07326977375649335
115	17	-0.05719479482852563
115	18	-0.07055914473838953
115	19	-0.09299348128267582
115	20	-0.11107422027401981
115	21	-0.09488727267435593
115	22	-0.11192325038099682
115	23	-0.0941395020608553
115	24	-0.08172284925644391
115	25	-0.09314117779398146
115	26	-0.10825353584684905
115	27	-0.07220320726074902
115	28	-0.10638605668481205
115	29	-0.10838356281755779
115	30	-0.10578519835053868
115	31	-0.10261120022841652
115	32	-0.12435950217714975
115	33	-0.08334027569952696
115	34	-0.09916002504839838
115	35	-0.09770789993190476
115	36	-0.09531885023542941
115	37	-0.08210540105162778
115	38	-0.10229512347913783
115	39	-0.08474339564158839
115	40	-0.10619123521524187
115	41	-0.06375862478525107
115	42	-0.07051855424978015
115	43	-0.08207633052150953
115	44	-0.07303438799731055
115	45	-0.10876014958879665
115	46	-0.062011386206876296
115	47	-0.08321565006085052
115	48	-0.06771265580794505
115	49	-0.03848987201359222
115	50	-0.05106668842159223
116	1	-0.0979320978221736
116	2	-0.1026908156851718
116	3	-0.0912392642827129
116	4	-0.10214108358433549
116	5	-0.07908133859975663
116	6	-0.08416202272638174
116	7	-0.0893856966667812
116	8	-0.08378778080214744
116	9	-0.07952147855400682
116	10	-0.0746284028938291
116	11	-0.09385867789602287
116	12	-0.1078522531880779
116	13	-0.09946118838081475
116	14	-0.107982106036836
116	15	-0.08726585996987524
116	16	-0.09126193856170772
116	17	-0.09282567864165969
116	18	-0.08253179324406545
116	19	-0.09541174168963806
116	20	-0.12101408432000227
116	21	-0.1145588461740985
116	22	-0.08409024696266698
116	23	-0.12986875555144906
116	24	-0.10554404296557607
116	25	-0.1086535175827521
116	26	-0.10044923159569663
116	27	-0.1193631751631159
116	28	-0.11507486674723566
116	29	-0.08792119286404443
116	30	-0.1322441013315938
116	31	-0.11248720133772139
116	32	-0.17422312415236263
116	33	-0.1244253382535021
116	34	-0.11808478863902394
116	35	-0.13887412972540858
116	36	-0.14721989789064338
116	37	-0.16321258484573065
116	38	-0.1520005619706808
116	39	-0.12736825465417922
116	40	-0.13636924543177997
116	41	-0.11818385962845213
116	42	-0.17088786550797153
116	43	-0.12979671463374132
116	44	-0.15749964429326013
116	45	-0.14796897496151473
116	46	-0.13117485749841507
116	47	-0.14820027864480778
116	48	-0.09557330281824505
116	49	-0.10480229706329425
116	50	-0.1281074310779573
117	1	-0.0759294023294359
117	2	-0.08175966620437691
117	3	-0.06805554273253023
117	4	-0.06681933866731526
117	5	-0.0652915973963033
117	6	-0.07095750377565935
117	7	-0.07631583186680047
117	8	-0.08177380868368544
117	9	-0.08880075050727498
117	10	-0.08676485619557894
117	11	-0.09153056790391645
117	12	-0.07529559266045993
117	13	-0.07758315231037471
117	14	-0.08379829672531969
117	15	-0.09174129460664915
117	16	-0.09454344326614006
117	17	-0.0897390087139982
117	18	-0.09777530546980984
117	19	-0.09445648350045843
117	20	-0.09147094679108392
117	21	-0.10199506048510187
117	22	-0.0992268605421529
117	23	-0.0993911827045153
117	24	-0.12837528334284087
117	25	-0.118604434039419
117	26	-0.10329853764753238
117	27	-0.11299462908421276
117	28	-0.12182986421446174
117	29	-0.12489273907700493
117	30	-0.09948655050776978
117	31	-0.14336466632898492
117	32	-0.1228677465808019
117	33	-0.13852926103022156
117	34	-0.12409488230342447
117	35	-0.1297970777854971
117	36	-0.07699994169789193
117	37	-0.08257891756360891
117	38	-0.1113972304686115
117	39	-0.0797638062771
117	40	-0.1038762708643759
117	41	-0.08079128938121727
117	42	-0.08184772014853081
117	43	-0.13483530009137468
117	44	-0.09979665588443964
117	45	-0.11973178198867629
117	46	-0.07441127992201046
117	47	-0.1238746484440938
117	48	-0.09192280160415578
117	49	-0.08491332120382822
117	50	-0.07893201864932564
118	1	-0.117758143713246
118	2	-0.13237302400887785
118	3	-0.11135550893740032
118	4	-0.11561529478795052
118	5	-0.11298978097334356
118	6	-0.112913902690714
118	7	-0.11430666162081593
118	8	-0.12335889378742593
118	9	-0.11561679378958445
118	10	-0.11484936886844821
118	11	-0.12638083155347601
118	12	-0.1172226820101443
118	13	-0.14357488899493376
118	14	-0.12164855838061328
118	15	-0.14872641017663568
118	16	-0.128573717135545
118	17	-0.1361154422648454
118	18	-0.13436561785000833
118	19	-0.1498715691365149
118	20	-0.15475880627084848
118	21	-0.15774884295461788
118	22	-0.17918195582124952
118	23	-0.1504168840301361
118	24	-0.14977157594207569
118	25	-0.1891740345926607
118	26	-0.22274065877277138
118	27	-0.19417173657404874
118	28	-0.22385801373098957
118	29	-0.20872495938012747
118	30	-0.20783996072464106
118	31	-0.22832209106348955
118	32	-0.23379046097238643
118	33	-0.2355270785368333
118	34	-0.21669717316213166
118	35	-0.24826179715937258
118	36	-0.1966179938497774
118	37	-0.209734476862252
118	38	-0.2401287994293071
118	39	-0.22798491079026206
118	40	-0.19346686811771718
118	41	-0.2044963461172955
118	42	-0.2175469241758421
118	43	-0.22532451027176137
118	44	-0.1905722088290389
118	45	-0.23968928265867362
118	46	-0.22317604879968655
118	47	-0.21806871593402377
118	48	-0.19356281491357663
118	49	-0.20717104748660375
118	50	-0.1888397102395558
119	1	-0.08881729205747746
119	2	-0.11325476617600572
119	3	-0.08976261940397023
119	4	-0.0885625900071858
119	5	-0.09002864730420157
119	6	-0.09596055772475864
119	7	-0.08720386395115298
119	8	-0.10301625818689075
119	9	-0.1171959688802815
119	10	-0.1268161626011377
119	11	-0.12026276063323849
119	12	-0.12505972244422736
119	13	-0.1268869163913493
119	14	-0.1372775171689682
119	15	-0.14547097665165865
119	16	-0.1541096186801525
119	17	-0.15397835811259597
119	18	-0.16375888678528128
119	19	-0.1568389975605129
119	20	-0.1569345686388175
119	21	-0.15313563450628465
119	22	-0.15264600023957087
119	23	-0.16292718094766911
119	24	-0.18724563533069016
119	25	-0.18773587295327587
119	26	-0.20057892349593232
119	27	-0.23053021533210938
119	28	-0.22577853324441577
119	29	-0.20147629319009752
119	30	-0.20845260129523524
119	31	-0.22679100069689895
119	32	-0.21635366837229242
119	33	-0.18181094029483091
119	34	-0.2322446659962054
119	35	-0.184485211804585
119	36	-0.2192596068570296
119	37	-0.20843365655850477
119	38	-0.20469689481653575
119	39	-0.21610782009494045
119	40	-0.2296932574321815
119	41	-0.22919834070141576
119	42	-0.22844201627973604
119	43	-0.2756233487904396
119	44	-0.2165829797170531
119	45	-0.2400448014780423
119	46	-0.25267176119501755
119	47	-0.2531385992417583
119	48	-0.20466856440508963
119	49	-0.21104003848375896
119	50	-0.24042636108440973
120	1	-0.10986288363908159
120	2	-0.14754773342026795
120	3	-0.1253947157175972
120	4	-0.11939550169839919
120	5	-0.12203416997745364
120	6	-0.13844606865992404
120	7	-0.1353206185576994
120	8	-0.13595357930825713
120	9	-0.1337745048385699
120	10	-0.13769748796051573
120	11	-0.13094820213424896
120	12	-0.1453646404343445
120	13	-0.1273472140266847
120	14	-0.1469865941229287
120	15	-0.1368173367760832
120	16	-0.16972500578931005
120	17	-0.17492677035027526
120	18	-0.22781751334164113
120	19	-0.16767207967741626
120	20	-0.17722483236787667
120	21	-0.17281075689296443
120	22	-0.17916049217419072
120	23	-0.1860873502521656
120	24	-0.17850329482618577
120	25	-0.18113767998382002
120	26	-0.2286739544779266
120	27	-0.2264713637433715
120	28	-0.29134700080938103
120	29	-0.2591281041161366
120	30	-0.2951878755335571
120	31	-0.250890537053138
120	32	-0.2756828688751938
120	33	-0.23742785815259992
120	34	-0.2235984259132735
120	35	-0.25666189824335106
120	36	-0.2097306791101019
120	37	-0.21504795409553168
120	38	-0.2289190980389577
120	39	-0.21702604907603984
120	40	-0.20897940731838105
120	41	-0.20851274220619587
120	42	-0.23285676104990216
120	43	-0.21084578856108038
120	44	-0.22425973686927136
120	45	-0.24021859311674376
120	46	-0.21940924553605612
120	47	-0.18118156818330533
120	48	-0.18498946835471486
120	49	-0.1541523658755395
120	50	-0.1788864560144603
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	5403.0
3	340.0
4	415.0
5	434.0
6	648.0
7	1288.0
8	1612.0
9	1808.0
10	2734.0
11	4538.0
12	4513.0
13	6068.0
14	8990.0
15	10936.0
16	11205.0
17	12848.0
18	16748.0
19	20804.0
20	27931.0
21	20974.0
22	25742.0
23	33642.0
24	35285.0
25	39175.0
26	52454.0
27	66583.0
28	76886.0
29	90826.0
30	112059.0
31	139229.0
32	167661.0
33	212338.0
34	294269.0
35	439373.0
36	717465.0
37	1373842.0
38	3021089.0
39	1.8134131E7
40	744384.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	17.585528276878296	28.8664556121521	24.531302411405985	29.016713699563613
2	24.155381490068972	29.112617137943086	25.920373959249364	20.811627412738577
3	23.633982311530353	27.643174702072393	25.534885550072545	23.18795743632471
4	23.142947803245367	26.6803255776474	26.7474968837557	23.42922973535153
5	23.982806872886364	27.613326828765	27.435185039174115	20.96868125917452
6	23.95228454539461	26.744894390837377	24.957024166942016	24.345796896826
7	23.585626065335298	27.568361705646872	26.607771930629493	22.23824029838834
8	23.765159521249256	26.722786695439314	26.63794157075677	22.874112212554657
9	22.570869737711124	26.152817613055184	27.158143277452346	24.11816937178134
10	22.753009542088478	27.918075065149072	26.05172522147215	23.2771901712903
11	23.70551038356119	27.108213968871098	26.15063923009392	23.035636417473793
12	23.522302593201054	26.706948116315626	26.581438557841082	23.18931073264224
13	22.80380634830917	27.722826407553473	26.261185418174342	23.21218182596301
14	22.903722798647628	28.016992929315908	26.387631874099487	22.691652397936977
15	22.63384218107766	26.954285749316227	27.260829717171966	23.151042352434146
16	23.64607921827467	26.810155609321946	26.420529852010304	23.12323532039308
17	23.149756695828724	26.674573104411632	26.50011354580214	23.675556653957504
18	22.683158632160566	26.628603440611304	26.4738804171854	24.21435751004273
19	23.884329298645703	26.788191680633705	26.373990362599848	22.953488658120747
20	23.156218589356307	27.429076284657977	26.577540601781184	22.837164524204535
21	23.669651173649292	26.73766775079926	26.46788383471863	23.124797240832816
22	23.051840628387424	27.34063353489171	26.404735238364186	23.202790598356675
23	22.831451424133277	26.630695267507015	27.67134272493142	22.866510583428287
24	22.808131478663814	26.56333950013404	26.58026179863791	24.048267222564238
25	23.838720989254632	26.478469650349055	26.3516011057092	23.33120825468711
26	23.894324220805355	26.57504519345142	26.677414010579252	22.85321657516397
27	23.044623603758552	27.204385042393746	26.623626516152786	23.127364837694916
28	23.101510314360794	27.339073906341376	26.454923566181176	23.10449221311665
29	23.940270881115254	26.564680732598667	26.5755433497696	22.919505036516487
30	22.9993432280392	27.23694192224107	26.56691185484576	23.19680299487397
31	23.91326778319677	26.46839700173177	26.48396468665849	23.134370528412973
32	23.05134702376429	26.52130693379427	26.554025410244254	23.87332063219719
33	23.848714379126896	26.383070515973046	26.496149866824897	23.27206523807516
34	23.954864921323775	26.45819947692565	26.44608223713334	23.140853364617236
35	23.067115337120274	26.576384601136084	27.37924487527785	22.977255186465793
36	23.015618370636385	26.39467820883293	27.380210957180058	23.20949246335063
37	23.13860552155722	26.465909547421436	26.363076480410065	24.032408450611282
38	23.077523338115068	27.47454759698835	26.47637290458859	22.971556160307987
39	23.905808252099703	26.39100851552174	26.47315423187252	23.230029000506033
40	23.11074839626285	26.423118944983457	26.372955368270656	24.093177290483037
41	23.137836751642702	27.39652395087243	26.489134495253232	22.976504802231638
42	23.951571665980143	26.336887972288732	26.49294990517326	23.218590456557866
43	23.958526250297783	26.437022428607488	26.399326317076394	23.20512500401833
44	23.134100860414286	27.37294904106713	26.513935685725425	22.979014412793155
45	23.056506694309302	27.177392952448827	26.501633107202483	23.26446724603939
46	23.122017537959803	26.43576128983286	27.232972519631343	23.209248652575994
47	24.03290777986599	26.491903922105447	26.474640277688344	23.00054802034022
48	23.066715183758486	26.299907051804865	26.451087339452023	24.18229042498463
49	23.11370572259476	26.44008878087074	26.32183474906385	24.124370747470657
50	24.049364170812147	26.46389199984433	26.407481695124446	23.079262134219075
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	6471.0
1	3719.5
2	968.0
3	787.0
4	606.0
5	614.5
6	623.0
7	868.0
8	1113.0
9	1912.0
10	2711.0
11	4952.0
12	7193.0
13	11990.0
14	16787.0
15	25559.0
16	34331.0
17	51475.5
18	68620.0
19	93038.0
20	117456.0
21	155118.5
22	192781.0
23	263777.0
24	334773.0
25	366569.0
26	398365.0
27	465742.5
28	533120.0
29	610578.0
30	688036.0
31	770372.5
32	852709.0
33	936903.5
34	1021098.0
35	1117588.0
36	1214078.0
37	1295044.5
38	1376011.0
39	1439298.5
40	1502586.0
41	1551026.0
42	1599466.0
43	1623483.0
44	1647500.0
45	1657080.0
46	1666660.0
47	1657792.5
48	1648925.0
49	1628301.5
50	1607678.0
51	1565958.0
52	1524238.0
53	1480375.0
54	1436512.0
55	1373459.0
56	1310406.0
57	1253375.5
58	1196345.0
59	1230163.0
60	1263981.0
61	1051185.5
62	838390.0
63	723895.5
64	609401.0
65	522011.0
66	434621.0
67	364185.5
68	293750.0
69	244103.5
70	194457.0
71	160931.0
72	127405.0
73	100952.0
74	74499.0
75	57728.5
76	40958.0
77	31973.5
78	22989.0
79	17818.5
80	12648.0
81	9771.0
82	6894.0
83	5343.0
84	3792.0
85	2915.0
86	2038.0
87	1701.0
88	1364.0
89	1018.5
90	673.0
91	481.0
92	289.0
93	196.5
94	104.0
95	85.5
96	67.0
97	53.0
98	39.0
99	92.0
100	145.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0691376340910379
2	0.0022439272273580223
3	0.0
4	0.0
5	1.0409971673310414E-4
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	3.7398787122633705E-4
16	0.0039056671500234995
17	0.0
18	0.0
19	2.698881544932329E-5
20	0.0
21	0.0028068368067296224
22	0.00611875001686801
23	0.0019624724376722225
24	0.0018390949956181729
25	0.0014458293990708907
26	0.022832537870127508
27	0.04343271514808956
28	0.052396857422329086
29	0.04973267578297446
30	0.04976737568855215
31	0.04307029391205579
32	0.048117202401079244
33	0.05120549399749467
34	0.05754401008302145
35	0.0889474246308412
36	0.09443000971211801
37	0.09175811698263502
38	0.09589897238157405
39	0.09792313354027328
40	0.09039710957497628
41	0.10308570838122241
42	0.10936639129078636
43	0.0932386462872836
44	0.0976031232999456
45	0.1224405446034514
46	0.14075823920341354
47	0.12539003657755604
48	0.1355031312809239
49	0.14071197266264326
50	0.13638605110062316
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2.593667E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.701588016063255
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.20926691780113	27.76225480966034
2	15.855654967986279	12.907006724636485
3	5.816981865803156	7.102811981964931
4	2.7154555444172535	4.4209341137922324
5	1.6246242615552469	3.3062393687361324
6	1.0741669377696192	2.623218009694518
7	0.7308056384397645	2.082146501091396
8	0.5289496486348558	1.7223272543981853
9	0.40780876326928994	1.4938617844734219
>10	2.6633899735415545	21.08380190530561
>50	0.2690899154119005	7.504921537943665
>100	0.10344879748715177	6.770489904977257
>500	2.5208545085728454E-4	0.06109443808719403
>1k	4.768210415762593E-5	0.04878528486013052
>5k	3.8000218553597145E-5	0.10398643968425664
>10k+	1.900010927674165E-5	1.006119940694099
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCG	217247	0.8376055985598768	Illumina Paired End PCR Primer 2 (100% over 31bp)
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	42956	0.16561879377730448	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.4699905577701376E-5	0.0	0.0	0.0	0.0
2	4.241099570607946E-5	3.855545064189042E-6	0.0	0.0	0.0
3	5.012208583445755E-5	3.855545064189042E-6	0.0	0.0	0.0
4	5.012208583445755E-5	3.855545064189042E-6	3.855545064189042E-6	0.0	0.0
5	6.168872102702468E-5	3.855545064189042E-6	3.855545064189042E-6	3.855545064189042E-6	0.0
6	6.939981115540275E-5	3.855545064189042E-6	3.855545064189042E-6	3.855545064189042E-6	0.0
7	9.253308154053701E-5	3.855545064189042E-6	3.855545064189042E-6	7.711090128378085E-6	0.0
8	1.4265516737499456E-4	3.855545064189042E-6	3.855545064189042E-6	7.711090128378085E-6	0.0
9	2.77599244621611E-4	3.855545064189042E-6	3.855545064189042E-6	7.711090128378085E-6	0.0
10	0.00173113973382088	3.855545064189042E-6	3.855545064189042E-6	7.711090128378085E-6	0.0
11	0.0021629607810100524	3.855545064189042E-6	3.855545064189042E-6	1.1566635192567126E-5	0.0
12	0.002741292540638409	3.855545064189042E-6	3.855545064189042E-6	1.1566635192567126E-5	0.0
13	0.003315768755202576	3.855545064189042E-6	3.855545064189042E-6	1.1566635192567126E-5	0.0
14	0.004044466772334305	3.855545064189042E-6	3.855545064189042E-6	1.1566635192567126E-5	0.0
15	0.01304330895215153	3.855545064189042E-6	3.855545064189042E-6	1.1566635192567126E-5	3.855545064189042E-6
16	0.014381183089425126	3.855545064189042E-6	7.711090128378085E-6	1.1566635192567126E-5	3.855545064189042E-6
17	0.017203442076411505	3.855545064189042E-6	7.711090128378085E-6	1.1566635192567126E-5	3.855545064189042E-6
18	0.02009510087455329	3.855545064189042E-6	7.711090128378085E-6	1.1566635192567126E-5	3.855545064189042E-6
19	0.025296231166144306	3.855545064189042E-6	7.711090128378085E-6	1.1566635192567126E-5	3.855545064189042E-6
20	0.897998856445334	3.855545064189042E-6	7.711090128378085E-6	1.1566635192567126E-5	7.711090128378085E-6
21	0.9111077096635767	3.855545064189042E-6	7.711090128378085E-6	1.1566635192567126E-5	1.1566635192567126E-5
22	0.9162432956890765	3.855545064189042E-6	7.711090128378085E-6	1.1566635192567126E-5	1.1566635192567126E-5
23	0.9202646291910257	3.855545064189042E-6	7.711090128378085E-6	1.542218025675617E-5	1.542218025675617E-5
24	0.922493134238127	3.855545064189042E-6	1.542218025675617E-5	1.542218025675617E-5	1.542218025675617E-5
25	0.9251071937916471	3.855545064189042E-6	1.542218025675617E-5	1.542218025675617E-5	1.542218025675617E-5
26	0.9277058311649106	3.855545064189042E-6	1.542218025675617E-5	1.542218025675617E-5	1.542218025675617E-5
27	0.9303931460746503	3.855545064189042E-6	1.542218025675617E-5	1.542218025675617E-5	1.542218025675617E-5
28	0.9602774758671796	3.855545064189042E-6	1.542218025675617E-5	1.542218025675617E-5	1.542218025675617E-5
29	0.9642563983734227	3.855545064189042E-6	1.542218025675617E-5	1.542218025675617E-5	1.542218025675617E-5
30	0.9680001326307502	3.855545064189042E-6	1.542218025675617E-5	1.542218025675617E-5	1.542218025675617E-5
31	0.996284411221641	3.855545064189042E-6	1.542218025675617E-5	1.542218025675617E-5	1.542218025675617E-5
32	0.9991645033845903	3.855545064189042E-6	1.542218025675617E-5	1.542218025675617E-5	1.542218025675617E-5
33	1.0015549413243874	3.855545064189042E-6	1.542218025675617E-5	1.542218025675617E-5	1.542218025675617E-5
34	1.0036832021998199	3.855545064189042E-6	1.542218025675617E-5	1.542218025675617E-5	1.542218025675617E-5
35	1.0059618293327555	3.855545064189042E-6	1.542218025675617E-5	1.542218025675617E-5	1.542218025675617E-5
36	1.008151778929215	3.855545064189042E-6	1.542218025675617E-5	1.542218025675617E-5	1.542218025675617E-5
37	1.0103070286200966	3.855545064189042E-6	1.542218025675617E-5	1.542218025675617E-5	1.542218025675617E-5
38	1.0127938551864986	7.711090128378085E-6	1.542218025675617E-5	1.542218025675617E-5	1.542218025675617E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATGCCGA	28425	0.0	35.262596	14
GATCGGA	28675	0.0	34.880436	21
CCGAGAT	28895	0.0	34.722202	17
CGAGATC	29035	0.0	34.52502	18
AGATCGG	29140	0.0	34.399902	20
TGCCGAG	29410	0.0	34.142635	15
GAGATCG	29660	0.0	33.797764	19
ATCGGAA	29610	0.0	33.721535	22
GCCGAGA	29860	0.0	33.637405	16
TCGGAAG	30095	0.0	33.140392	23
AAGAGCG	30235	0.0	32.821243	27
AGAGCGG	30435	0.0	32.62857	28
GAGCGGT	30645	0.0	32.355476	29
AGCGGTT	31290	0.0	31.697868	30
GCGGTTC	31320	0.0	31.6471	31
CGGAAGA	33455	0.0	29.831596	24
GAAGAGC	41325	0.0	24.342377	26
GGAAGAG	46755	0.0	21.679739	25
AATGCCG	50200	0.0	19.975677	13
CGGTTCA	52650	0.0	19.189793	1
>>END_MODULE