FastQCFastQC Report
Thu 26 May 2016
SRR522126_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR522126_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28648368
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCG2361350.8242528858886482Illumina Paired End PCR Primer 2 (100% over 31bp)
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT640510.223576435488402No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA298800.036.64699621
AGATCGG302350.036.2604220
ATGCCGA308950.035.52583314
ATCGGAA309700.035.3087722
TGCCGAG314650.034.93374315
GAGATCG316800.034.65464819
CCGAGAT318950.034.48902517
CGAGATC319250.034.39541218
TCGGAAG321450.034.02514323
GAGCGGT320550.033.85640329
AAGAGCG322450.033.7583127
AGAGCGG323600.033.62544628
GCCGAGA328350.033.49649416
GCGGTTC328150.033.04990431
AGCGGTT331100.032.7149330
CGGAAGA359250.030.4577724
GAAGAGC442650.024.88389626
GGAAGAG501650.022.1498225
GAGTACT175500.020.10040720
AATGCCG546500.020.07960913
Produced by FastQC (version 0.11.5)