##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR522126_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 28648368 Sequences flagged as poor quality 0 Sequence length 50 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 39.28231157879569 40.0 39.0 40.0 38.0 40.0 2 39.20944641593545 40.0 39.0 40.0 38.0 40.0 3 39.20276949109282 40.0 39.0 40.0 38.0 40.0 4 39.20341207568962 40.0 39.0 40.0 38.0 40.0 5 39.19993512370408 40.0 39.0 40.0 38.0 40.0 6 39.23187488376301 40.0 39.0 40.0 38.0 40.0 7 39.20196808418546 40.0 39.0 40.0 38.0 40.0 8 39.191334878133375 40.0 39.0 40.0 38.0 40.0 9 39.17664381440507 40.0 39.0 40.0 38.0 40.0 10 39.161838747673166 40.0 39.0 40.0 38.0 40.0 11 39.172909256122374 40.0 39.0 40.0 38.0 40.0 12 39.1434135794402 40.0 39.0 40.0 38.0 40.0 13 39.114643703264356 40.0 39.0 40.0 38.0 40.0 14 39.09942744382507 40.0 39.0 40.0 38.0 40.0 15 39.081061476172046 40.0 39.0 40.0 38.0 40.0 16 39.06309661339173 40.0 39.0 40.0 38.0 40.0 17 39.03183615904403 40.0 39.0 40.0 38.0 40.0 18 39.00357971525638 40.0 39.0 40.0 38.0 40.0 19 38.969026089025384 40.0 39.0 40.0 38.0 40.0 20 38.92420123198641 40.0 39.0 40.0 37.0 40.0 21 38.893223097385516 40.0 39.0 40.0 37.0 40.0 22 38.84559291475173 40.0 39.0 40.0 37.0 40.0 23 38.81558031508112 40.0 39.0 40.0 37.0 40.0 24 38.77855646087763 40.0 39.0 40.0 36.0 40.0 25 38.734797807679655 40.0 39.0 40.0 36.0 40.0 26 38.61919429406939 40.0 39.0 40.0 36.0 40.0 27 38.53041126112315 40.0 39.0 40.0 36.0 40.0 28 38.47860747250943 40.0 39.0 40.0 36.0 40.0 29 38.43257224983985 40.0 38.0 40.0 35.0 40.0 30 38.338426153978475 40.0 38.0 40.0 35.0 40.0 31 38.27948862566971 40.0 38.0 40.0 35.0 40.0 32 38.17149615643027 40.0 38.0 40.0 35.0 40.0 33 38.416215995270655 40.0 39.0 40.0 36.0 40.0 34 38.380607998333446 40.0 39.0 40.0 36.0 40.0 35 38.3138530613681 40.0 39.0 40.0 35.0 40.0 36 38.38824319765789 40.0 39.0 40.0 36.0 40.0 37 38.329550884015454 40.0 39.0 40.0 36.0 40.0 38 38.26984060662723 40.0 39.0 40.0 35.0 40.0 39 38.24413669916555 40.0 39.0 40.0 35.0 40.0 40 38.180025158850235 40.0 39.0 40.0 35.0 40.0 41 38.11010676070623 40.0 39.0 40.0 35.0 40.0 42 38.0440713411668 40.0 38.0 40.0 35.0 40.0 43 37.9748242901655 40.0 38.0 40.0 35.0 40.0 44 37.91342927457508 40.0 38.0 40.0 35.0 40.0 45 37.862628509938155 40.0 38.0 40.0 35.0 40.0 46 37.75032926831992 40.0 38.0 40.0 35.0 40.0 47 37.69622793172721 40.0 38.0 40.0 35.0 40.0 48 37.70564068431403 40.0 38.0 40.0 35.0 40.0 49 37.647698326131525 40.0 38.0 40.0 34.0 40.0 50 37.58675949708549 40.0 38.0 40.0 34.0 40.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1 1 -0.10471304823855121 1 2 -0.14582873840478783 1 3 -0.12036837237339171 1 4 -0.11786470326908471 1 5 -0.12117583969725132 1 6 -0.13432227628033644 1 7 -0.147049589855186 1 8 -0.12876319630903765 1 9 -0.12891820619004335 1 10 -0.1447829429413261 1 11 -0.141233741730737 1 12 -0.1639830225586678 1 13 -0.15066160311138077 1 14 -0.1559536830356194 1 15 -0.1375119021509974 1 16 -0.16706041287884688 1 17 -0.17542967915814245 1 18 -0.17654586802385097 1 19 -0.21169158529114895 1 20 -0.2588530306944392 1 21 -0.17831272275461174 1 22 -0.20152737205327753 1 23 -0.20798715581555882 1 24 -0.18683197607098379 1 25 -0.1608457931825953 1 26 -0.17351641152819752 1 27 -0.16700124840362207 1 28 -0.14824882576657217 1 29 -0.16530272632686405 1 30 -0.1904675478853335 1 31 -0.14287719145993805 1 32 -0.1800554290777754 1 33 -0.14608878412015258 1 34 -0.1694567107709375 1 35 -0.16114494277282887 1 36 -0.13765399074051032 1 37 -0.13522300005737975 1 38 -0.15709381925010746 1 39 -0.14449757585668266 1 40 -0.16356435103936207 1 41 -0.1552358261311042 1 42 -0.15333183979176823 1 43 -0.13336205776678867 1 44 -0.14052686457875296 1 45 -0.24166926825829904 1 46 -0.1579543238578225 1 47 -0.1423181269438345 1 48 -0.10293111989160053 1 49 -0.08863235419016746 1 50 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-0.1867048828925988 119 39 -0.19691342400655287 119 40 -0.1800348018378628 119 41 -0.2105451202212265 119 42 -0.22835340884680022 119 43 -0.21868387096704822 119 44 -0.21609746168737587 119 45 -0.22208097684566042 119 46 -0.23832429696945212 119 47 -0.21244986173508096 119 48 -0.1664728654025751 119 49 -0.16904897151878373 119 50 -0.15625914108255046 120 1 -0.11649315511653668 120 2 -0.14688670919225189 120 3 -0.1334650999311009 120 4 -0.1376313053918352 120 5 -0.136286676912313 120 6 -0.13534110752921436 120 7 -0.14529907046043888 120 8 -0.1525263045292249 120 9 -0.16195891749036662 120 10 -0.144406005422546 120 11 -0.1778472880017219 120 12 -0.1537090198065414 120 13 -0.1575129180432313 120 14 -0.17763956417356042 120 15 -0.1388228925490722 120 16 -0.16185352865880276 120 17 -0.14245031446832712 120 18 -0.17456056184814628 120 19 -0.13775480482796354 120 20 -0.19522066457639653 120 21 -0.16371839198913563 120 22 -0.14624993021311639 120 23 -0.1493928827655182 120 24 -0.15887086224689284 120 25 -0.17706547426093522 120 26 -0.17495725534193696 120 27 -0.17380195007999788 120 28 -0.16997618987126373 120 29 -0.16090717401729648 120 30 -0.19530975785463056 120 31 -0.1968425367736657 120 32 -0.15559600990034994 120 33 -0.17533654216696704 120 34 -0.1812160484177454 120 35 -0.17818341109204283 120 36 -0.16478776847762333 120 37 -0.180546242674815 120 38 -0.18155603717192292 120 39 -0.1830243924820678 120 40 -0.1718320828887201 120 41 -0.11054465956625847 120 42 -0.11709918636287142 120 43 -0.11339176658360373 120 44 -0.15072304356995403 120 45 -0.11705100551650816 120 46 -0.11179034800920817 120 47 -0.10815425769724385 120 48 -0.07145862869856501 120 49 -0.06743877963306488 120 50 -0.05971548712096819 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5336.0 3 481.0 4 531.0 5 547.0 6 851.0 7 1597.0 8 2050.0 9 2480.0 10 3463.0 11 5731.0 12 5942.0 13 7802.0 14 10886.0 15 13860.0 16 14081.0 17 16059.0 18 20952.0 19 25201.0 20 33362.0 21 25098.0 22 31600.0 23 40413.0 24 44150.0 25 48955.0 26 64748.0 27 82776.0 28 95344.0 29 113018.0 30 138516.0 31 172397.0 32 208473.0 33 266643.0 34 370148.0 35 551767.0 36 890419.0 37 1683417.0 38 3534470.0 39 1.9491026E7 40 623778.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 17.52842352657806 28.832588268118414 24.576925113506192 29.062063091797334 2 24.24919111812974 28.987900195374337 25.781499093989474 20.981409592506452 3 23.960252254517684 27.557183276798984 25.308884098001116 23.17368037068221 4 23.11393095760289 26.544971078282714 26.731746115520437 23.60935184859396 5 24.060190793416226 27.495178782958945 27.41000464668703 21.034625776937798 6 24.08973872438388 26.54479305767086 25.004143342475913 24.361324875469347 7 23.693525386831354 27.422761048922318 26.60752350127688 22.27619006296945 8 23.948341490167955 26.603257120964095 26.541365288242595 22.907036100625348 9 22.754224603649327 26.02223973107299 27.08673666855997 24.13679899671772 10 22.81934524158584 27.699183422944024 26.136762834099308 23.344708501370828 11 23.73794207055704 27.047488359546346 26.10542771581264 23.109141854083976 12 23.569890612966155 26.55644119064653 26.600970777811845 23.272697418575465 13 22.869948473155606 27.56379700232837 26.14833417386987 23.417920350646153 14 23.011440142084357 27.954568944727082 26.244801050029682 22.789189863158878 15 22.740006777361714 26.941290371133856 27.08894302897245 23.229759822531978 16 23.732934065851207 26.68040928919646 26.263013171861417 23.323643473090915 17 23.381063102791757 26.508099868027386 26.3417692763511 23.76906775282976 18 22.78560816216853 26.49388644052053 26.320861402748974 24.39964399456197 19 23.972370782168113 26.768477701766468 26.206567857547768 23.05258365851765 20 23.36372241973032 27.282575219996218 26.412960921670383 22.940741438603077 21 23.78044206227307 26.694727563119653 26.306910647081683 23.217919727525594 22 23.23627347996071 27.179247774879027 26.282022618478017 23.30245612668224 23 22.919765234376595 26.470445017764916 27.65076217217228 22.959027575686207 24 22.93397424013473 26.521653032142883 26.433387789048513 24.110984938673877 25 23.920504178419318 26.31109305271139 26.25790920383227 23.51049356503703 26 23.96276304727985 26.417156407589115 26.674225439359773 22.94585510577126 27 23.12310271555079 27.049360545646856 26.621973913369008 23.205562825433347 28 23.201005130425976 27.173421669326757 26.437731157296973 23.18784204295029 29 24.034441212978933 26.398932432476325 26.55785851168121 23.008767842863538 30 23.103565224142326 27.074756576658675 26.549840645594102 23.271837553604893 31 23.974799805024098 26.328144103365442 26.47260898022003 23.224447111390425 32 23.156249465231586 26.35240269484866 26.537309460922003 23.954038378997755 33 23.94334848953892 26.244851462852303 26.464776629902108 23.347023417706662 34 24.024833597066372 26.319798258282724 26.430122922170224 23.225245222480677 35 23.17538004294557 26.4155791606479 27.33113601458485 23.077904781821676 36 23.12031824156393 26.249195376338857 27.324287540098414 23.3061988419988 37 23.208671731840905 26.33557753541338 26.35468557732753 24.10106515541819 38 23.167305481696815 27.290705678058192 26.478950549776908 23.06303829046809 39 23.976379120710362 26.233923074936737 26.45621922737673 23.333478576976166 40 23.21072519512304 26.27656453759051 26.34467434678661 24.168035920499843 41 23.21759186783868 27.220382874446074 26.47134116477562 23.090684092939625 42 24.050569660300393 26.19097321178605 26.44866320303215 23.309793924881408 43 24.046894653612874 26.285105429399376 26.366842448131845 23.30115746885591 44 23.23126045181908 27.206044007879964 26.481413669459407 23.081281870841547 45 23.162825320276053 27.059050750333473 26.422353019945295 23.35577090944518 46 23.192005655320784 26.305427517609527 27.199349183727143 23.303217643342546 47 24.083693216213533 26.337418685575837 26.44755053622433 23.1313375619863 48 23.141994266071002 26.152205620547956 26.410340087887434 24.295460025493608 49 23.217606983675662 26.285909340924874 26.28085468439246 24.21562899100701 50 24.124149613199904 26.326594320253484 26.365742181761355 23.183513884785256 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 7389.0 1 4122.5 2 856.0 3 758.5 4 661.0 5 734.5 6 808.0 7 1094.5 8 1381.0 9 2265.0 10 3149.0 11 5702.0 12 8255.0 13 14030.0 14 19805.0 15 28673.5 16 37542.0 17 55978.5 18 74415.0 19 102084.5 20 129754.0 21 171695.5 22 213637.0 23 299403.0 24 385169.0 25 406944.5 26 428720.0 27 504336.0 28 579952.0 29 659876.0 30 739800.0 31 827221.0 32 914642.0 33 1007007.0 34 1099372.0 35 1201628.0 36 1303884.0 37 1391329.5 38 1478775.0 39 1545767.0 40 1612759.0 41 1667023.0 42 1721287.0 43 1752686.0 44 1784085.0 45 1802132.0 46 1820179.0 47 1821257.0 48 1822335.0 49 1807144.5 50 1791954.0 51 1755160.5 52 1718367.0 53 1678156.0 54 1637945.0 55 1569222.5 56 1500500.0 57 1437233.5 58 1373967.0 59 1405225.5 60 1436484.0 61 1195902.5 62 955321.0 63 827357.0 64 699393.0 65 596430.5 66 493468.0 67 411822.0 68 330176.0 69 273071.5 70 215967.0 71 177069.0 72 138171.0 73 107914.5 74 77658.0 75 59588.0 76 41518.0 77 32208.0 78 22898.0 79 17478.5 80 12059.0 81 9124.5 82 6190.0 83 4833.0 84 3476.0 85 2678.0 86 1880.0 87 1533.5 88 1187.0 89 871.0 90 555.0 91 413.5 92 272.0 93 191.0 94 110.0 95 84.5 96 59.0 97 49.0 98 39.0 99 91.0 100 143.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.06357081143330748 2 0.0027854989854919484 3 3.4906002324460506E-6 4 0.0 5 0.0 6 0.0 7 1.0820860720582757E-4 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 1.2217100813561178E-4 15 1.8151121208719465E-4 16 0.004328344288233103 17 0.0 18 0.0017138847141310108 19 0.0 20 1.2217100813561178E-4 21 0.0064960070325821 22 0.001308975087167269 23 1.7802061185474857E-4 24 0.0021816251452787816 25 0.0020001139331915874 26 0.02455288203502552 27 0.044344585352994625 28 0.05329448434898631 29 0.04991907392421097 30 0.05243230609157213 31 0.04549648342970182 32 0.050536910165353915 33 0.05233107868483119 34 0.05763330043791674 35 0.08620037274025522 36 0.09719576347246028 37 0.09393554285535567 38 0.09657792723131732 39 0.1039814903243354 40 0.09403677026209661 41 0.10728708874446181 42 0.1056255630338175 43 0.09105579766358768 44 0.10104589552884828 45 0.10992598252019103 46 0.13314196466618972 47 0.12987825344885268 48 0.1410551553931449 49 0.1434043293495811 50 0.1357389712391296 >>END_MODULE >>Sequence Length Distribution pass #Length Count 50 2.8648368E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 41.03358514895239 #Duplication Level Percentage of deduplicated Percentage of total 1 66.61764212614901 27.335606906057723 2 16.67393080035402 13.68382318528133 3 6.118500118154035 7.531919867464465 4 2.960828900824466 4.859736992538393 5 1.7851405086212544 3.662535753167721 6 1.1430780390040194 2.814275402722137 7 0.7733722438642242 2.2213965074307307 8 0.5722592693188766 1.878547956389872 9 0.43201084692880987 1.595425848545192 >10 2.5725854139206525 20.140344906082653 >50 0.2622919423652671 7.395363952686535 >100 0.0880248770545599 5.606249788665911 >500 2.236569612575313E-4 0.053522196049036116 >1k 5.159110021261045E-5 0.041849174142525425 >5k 4.261812810298212E-5 0.12948626562299675 >10k+ 1.7047251240611345E-5 1.0499152971526784 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCG 236135 0.8242528858886482 Illumina Paired End PCR Primer 2 (100% over 31bp) AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 64051 0.223576435488402 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 1.3962400929784202E-5 1.3962400929784202E-5 0.0 0.0 0.0 2 3.141540209201446E-5 1.3962400929784202E-5 0.0 3.4906002324460506E-6 0.0 3 4.886840325424471E-5 1.3962400929784202E-5 0.0 3.4906002324460506E-6 3.4906002324460506E-6 4 5.235900348669076E-5 1.7453001162230252E-5 0.0 3.4906002324460506E-6 3.4906002324460506E-6 5 6.981200464892101E-5 2.4434201627122354E-5 0.0 3.4906002324460506E-6 3.4906002324460506E-6 6 8.377440557870521E-5 3.141540209201446E-5 0.0 3.4906002324460506E-6 3.4906002324460506E-6 7 1.0820860720582757E-4 3.141540209201446E-5 0.0 3.4906002324460506E-6 3.4906002324460506E-6 8 1.2915220860050387E-4 3.141540209201446E-5 0.0 6.981200464892101E-6 3.4906002324460506E-6 9 1.8151121208719463E-4 3.141540209201446E-5 0.0 6.981200464892101E-6 3.4906002324460506E-6 10 0.001452089696697557 3.8396602556906554E-5 0.0 6.981200464892101E-6 3.4906002324460506E-6 11 0.001658035110411874 3.8396602556906554E-5 0.0 1.0471800697338151E-5 3.4906002324460506E-6 12 0.002007095133656479 4.1887202789352605E-5 0.0 1.0471800697338151E-5 3.4906002324460506E-6 13 0.002195587546208566 4.1887202789352605E-5 0.0 1.0471800697338151E-5 3.4906002324460506E-6 14 0.002467854364339358 4.886840325424471E-5 0.0 1.0471800697338151E-5 3.4906002324460506E-6 15 0.010792935918723188 5.235900348669076E-5 0.0 1.0471800697338151E-5 3.4906002324460506E-6 16 0.011267657550335852 5.235900348669076E-5 0.0 1.0471800697338151E-5 3.4906002324460506E-6 17 0.012800031052379668 5.584960371913681E-5 0.0 1.0471800697338151E-5 3.4906002324460506E-6 18 0.01454882176883514 5.584960371913681E-5 0.0 1.0471800697338151E-5 3.4906002324460506E-6 19 0.018960940462646948 5.584960371913681E-5 0.0 1.0471800697338151E-5 3.4906002324460506E-6 20 0.8786434187106226 6.283080418402892E-5 0.0 1.0471800697338151E-5 3.4906002324460506E-6 21 0.8907732545183725 6.632140441647496E-5 0.0 1.0471800697338151E-5 3.4906002324460506E-6 22 0.8946548019768525 6.981200464892101E-5 0.0 1.0471800697338151E-5 3.4906002324460506E-6 23 0.8964175550942378 6.981200464892101E-5 0.0 1.3962400929784202E-5 3.4906002324460506E-6 24 0.8972238837479328 7.679320511381311E-5 0.0 1.7453001162230252E-5 3.4906002324460506E-6 25 0.8977404925823349 7.679320511381311E-5 0.0 1.7453001162230252E-5 3.4906002324460506E-6 26 0.8985642742371921 8.377440557870521E-5 0.0 1.7453001162230252E-5 3.4906002324460506E-6 27 0.8993706028908872 8.726500581115127E-5 0.0 1.7453001162230252E-5 3.4906002324460506E-6 28 0.9288661748550563 8.726500581115127E-5 0.0 1.7453001162230252E-5 3.4906002324460506E-6 29 0.9305032663640735 8.726500581115127E-5 0.0 1.7453001162230252E-5 3.4906002324460506E-6 30 0.9325522487005193 9.075560604359731E-5 0.0 1.7453001162230252E-5 3.4906002324460506E-6 31 0.9577404199778501 9.424620627604337E-5 0.0 1.7453001162230252E-5 3.4906002324460506E-6 32 0.9590668480661796 1.0122740674093547E-4 0.0 1.7453001162230252E-5 3.4906002324460506E-6 33 0.9598277989168528 1.0471800697338152E-4 0.0 2.0943601394676303E-5 3.4906002324460506E-6 34 0.9604561069586931 1.0820860720582757E-4 0.0 2.4434201627122354E-5 3.4906002324460506E-6 35 0.9610146029958845 1.1169920743827362E-4 0.0 2.4434201627122354E-5 3.4906002324460506E-6 36 0.9615626272323785 1.1169920743827362E-4 0.0 2.4434201627122354E-5 3.4906002324460506E-6 37 0.9621944258744513 1.1868040790316572E-4 0.0 2.7924801859568405E-5 3.4906002324460506E-6 38 0.9628785835200107 1.2566160836805784E-4 0.0 2.7924801859568405E-5 3.4906002324460506E-6 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GATCGGA 29880 0.0 36.646996 21 AGATCGG 30235 0.0 36.26042 20 ATGCCGA 30895 0.0 35.525833 14 ATCGGAA 30970 0.0 35.30877 22 TGCCGAG 31465 0.0 34.933743 15 GAGATCG 31680 0.0 34.654648 19 CCGAGAT 31895 0.0 34.489025 17 CGAGATC 31925 0.0 34.395412 18 TCGGAAG 32145 0.0 34.025143 23 GAGCGGT 32055 0.0 33.856403 29 AAGAGCG 32245 0.0 33.75831 27 AGAGCGG 32360 0.0 33.625446 28 GCCGAGA 32835 0.0 33.496494 16 GCGGTTC 32815 0.0 33.049904 31 AGCGGTT 33110 0.0 32.71493 30 CGGAAGA 35925 0.0 30.45777 24 GAAGAGC 44265 0.0 24.883896 26 GGAAGAG 50165 0.0 22.14982 25 GAGTACT 17550 0.0 20.100407 20 AATGCCG 54650 0.0 20.079609 13 >>END_MODULE