FastQCFastQC Report
Thu 26 May 2016
SRR522125_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR522125_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27417401
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCG1405250.5125394635326668Illumina Paired End PCR Primer 2 (100% over 31bp)
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT837150.30533528688587225No Hit
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAG302620.11037515919178481Illumina Paired End PCR Primer 2 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGATCGG216450.029.91789820
ATGCCGA219100.029.49790614
TGCCGAG220850.029.31402615
CGAGATC233850.027.65433718
CCGAGAT237650.027.20303717
GAGATCG240800.026.89266419
GCCGAGA242950.026.70023516
GATCGGA243600.026.4118421
ATCGGAA252300.025.43129522
AGAGCGG253300.025.2248628
TCGGAAG259100.024.82328623
GAGCGGT257400.024.68643429
AAGAGCG261350.024.41413127
GCGGTTC261300.024.3600831
AGCGGTT266600.023.90056230
AAGCAGT251200.022.465491
GAGTACT204750.022.45294420
CAACGCA242200.022.01605213
TCAACGC247250.021.63803312
ATCAACG248550.021.51596811
Produced by FastQC (version 0.11.5)