##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR522125_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	27417401
Sequences flagged as poor quality	0
Sequence length	50
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	39.261923221679545	40.0	39.0	40.0	38.0	40.0
2	39.19587885080719	40.0	39.0	40.0	38.0	40.0
3	39.173100178240816	40.0	39.0	40.0	38.0	40.0
4	39.04607581148921	40.0	39.0	40.0	38.0	40.0
5	39.178693049716856	40.0	39.0	40.0	38.0	40.0
6	39.23147332600927	40.0	39.0	40.0	38.0	40.0
7	39.20767789040252	40.0	39.0	40.0	38.0	40.0
8	39.19454261911988	40.0	39.0	40.0	38.0	40.0
9	39.17915410727662	40.0	39.0	40.0	38.0	40.0
10	39.16386545901999	40.0	39.0	40.0	38.0	40.0
11	39.189287306991645	40.0	39.0	40.0	38.0	40.0
12	39.15761855035056	40.0	39.0	40.0	38.0	40.0
13	39.13987959690271	40.0	39.0	40.0	38.0	40.0
14	39.119975522114586	40.0	39.0	40.0	38.0	40.0
15	39.09794805131238	40.0	39.0	40.0	38.0	40.0
16	39.101234540794	40.0	39.0	40.0	38.0	40.0
17	39.06592663542398	40.0	39.0	40.0	38.0	40.0
18	39.0360759212735	40.0	39.0	40.0	38.0	40.0
19	39.01105338175562	40.0	39.0	40.0	38.0	40.0
20	38.978370852875514	40.0	39.0	40.0	38.0	40.0
21	38.94044844002537	40.0	39.0	40.0	37.0	40.0
22	38.88490214663308	40.0	39.0	40.0	37.0	40.0
23	38.80789725473979	40.0	39.0	40.0	36.0	40.0
24	38.74112174235625	40.0	39.0	40.0	36.0	40.0
25	38.745945649625945	40.0	39.0	40.0	36.0	40.0
26	38.62830284314695	40.0	39.0	40.0	36.0	40.0
27	38.54820327426367	40.0	39.0	40.0	36.0	40.0
28	38.51634117325709	40.0	39.0	40.0	36.0	40.0
29	38.48975831078956	40.0	38.0	40.0	35.0	40.0
30	38.40844254347814	40.0	38.0	40.0	35.0	40.0
31	38.36402538665135	40.0	38.0	40.0	35.0	40.0
32	38.293581109310836	40.0	38.0	40.0	35.0	40.0
33	38.259618079773496	40.0	38.0	40.0	35.0	40.0
34	38.20954739656031	40.0	38.0	40.0	35.0	40.0
35	38.13192603485648	40.0	38.0	40.0	35.0	40.0
36	38.13306786445586	40.0	38.0	40.0	35.0	40.0
37	38.377090155263076	40.0	38.0	40.0	35.0	40.0
38	38.31635263313251	40.0	38.0	40.0	35.0	40.0
39	38.28767730391367	40.0	38.0	40.0	35.0	40.0
40	38.23495990739604	40.0	38.0	40.0	35.0	40.0
41	38.29979413438932	40.0	39.0	40.0	35.0	40.0
42	38.24080703346025	40.0	38.0	40.0	35.0	40.0
43	38.226258462645674	40.0	38.0	40.0	35.0	40.0
44	38.167597541429984	40.0	38.0	40.0	35.0	40.0
45	38.08937269437026	40.0	38.0	40.0	35.0	40.0
46	37.97950597140845	40.0	38.0	40.0	35.0	40.0
47	37.87006492701478	40.0	38.0	40.0	35.0	40.0
48	37.73164440349397	40.0	38.0	40.0	34.0	40.0
49	37.688882764635494	40.0	38.0	40.0	34.0	40.0
50	37.68157149541636	40.0	38.0	40.0	34.0	40.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1	1	-0.0772814308678278
1	2	-0.10951927371296222
1	3	-0.11574161282975126
1	4	-0.25416968158216235
1	5	-0.07444833310703558
1	6	-0.1105924761296535
1	7	-0.12391362187825194
1	8	-0.11818118291871116
1	9	-0.13012638096347473
1	10	-0.11376360970461974
1	11	-0.10916631678752253
1	12	-0.13252590191062552
1	13	-0.11707914420114918
1	14	-0.12126482940909256
1	15	-0.11469288594338423
1	16	-0.11913851384076679
1	17	-0.13958545880535667
1	18	-0.1449005373711003
1	19	-0.12770464443373442
1	20	-0.14549749893735253
1	21	-0.114517843301293
1	22	-0.10934077877623594
1	23	-0.10064959199984003
1	24	-0.06697358650112761
1	25	-0.09975261805066538
1	26	-0.03251424742239095
1	27	-0.05404758901216411
1	28	-0.04567446240979933
1	29	-0.058878674064352765
1	30	-0.05200812148410705
1	31	-0.05717023748356098
1	32	-0.08442808668751667
1	33	-0.08005449857098057
1	34	-0.07114246732062668
1	35	-0.07368318205660529
1	36	-0.10685415082082983
1	37	-0.10319613526211668
1	38	-0.0818852851818832
1	39	-0.12071513749565099
1	40	-0.12187938656136055
1	41	-0.08300209071239806
1	42	-0.06183362253986502
1	43	-0.0324506757549301
1	44	-0.029265519884816626
1	45	0.003052112135328855
1	46	0.026949197160156757
1	47	0.0166849485718501
1	48	0.06850124274978953
1	49	0.12727790421164542
1	50	0.04516217035006065
2	1	-0.057790319121409084
2	2	-0.08581132406533243
2	3	-0.1620180418980368
2	4	-0.04881842903370881
2	5	-0.10140594738668085
2	6	-0.10323384549272419
2	7	-0.09111155309730634
2	8	-0.09587373459654458
2	9	-0.10066274096293171
2	10	-0.10050539045441553
2	11	-0.1101130948364144
2	12	-0.11542359588180773
2	13	-0.1264931480683984
2	14	-0.1267615520487979
2	15	-0.13311668021655976
2	16	-0.1191495252306396
2	17	-0.1016545101080979
2	18	-0.11635283752627146
2	19	-0.13190943496562113
2	20	-0.13036980832546163
2	21	-0.13746782322687068
2	22	-0.12814646222523862
2	23	-0.12127743898747667
2	24	-0.1457894942814093
2	25	-0.16226615883795859
2	26	-0.08612166719581893
2	27	-0.14814822474203027
2	28	-0.11725166596345815
2	29	-0.16160164245613373
2	30	-0.10154317890730624
2	31	-0.1451904040167591
2	32	-0.09566254079771852
2	33	-0.11764275098123989
2	34	-0.11199971073820336
2	35	-0.14163513770216696
2	36	-0.14036365781849724
2	37	-0.19001702197393655
2	38	-0.15773623977550244
2	39	-0.1705418552856841
2	40	-0.17990475999872757
2	41	-0.17358029199286307
2	42	-0.1661635050899406
2	43	-0.14968213627032867
2	44	-0.1313777090861521
2	45	-0.10449269043935061
2	46	-0.05199433185711655
2	47	-0.09664654228988923
2	48	-0.059569846531736914
2	49	-0.008127112240920553
2	50	-0.04511512391109562
3	1	-0.08099986334352138
3	2	-0.09373041051891562
3	3	-0.09812059413947338
3	4	-0.06757643876237296
3	5	-0.07220575600288726
3	6	-0.07264245838489103
3	7	-0.07862848981822879
3	8	-0.08480623080883021
3	9	-0.10018600126027621
3	10	-0.0939389073776411
3	11	-0.09027851996737724
3	12	-0.10048270742563403
3	13	-0.10681352943895916
3	14	-0.11541600919886008
3	15	-0.089584295559483
3	16	-0.11471664472437482
3	17	-0.09778912437418086
3	18	-0.0943891917969566
3	19	-0.12510213634107004
3	20	-0.11259712666358723
3	21	-0.10048943468794391
3	22	-0.12974994643938942
3	23	-0.1391969905820858
3	24	-0.09119883526446415
3	25	-0.12065229380372244
3	26	-0.11832380934981046
3	27	-0.059371926621032856
3	28	-0.13638017504019473
3	29	-0.10239521667974572
3	30	-0.12427039451162614
3	31	-0.09686351507535562
3	32	-0.14414922640442995
3	33	-0.12920038864601224
3	34	-0.14050257792884224
3	35	-0.11812505896163827
3	36	-0.14656802491989396
3	37	-0.16568749440056507
3	38	-0.1766061064417812
3	39	-0.1635151982953289
3	40	-0.17591145975369216
3	41	-0.13695321293135976
3	42	-0.17702740228228464
3	43	-0.139819157276456
3	44	-0.10693143463848287
3	45	-0.12457959361358917
3	46	-0.0800371489116074
3	47	-0.07865454594836763
3	48	-0.029646305657458072
3	49	-0.033209544443238315
3	50	-0.19026354119280597
4	1	-0.07335574407338186
4	2	-0.10463083024767883
4	3	-0.14002560117111784
4	4	-0.06791116993390034
4	5	-0.08087380188672455
4	6	-0.09469040137818752
4	7	-0.07515540365900364
4	8	-0.0959139606190007
4	9	-0.10085116781159087
4	10	-0.08705987438941776
4	11	-0.09537068129227322
4	12	-0.10437278746505285
4	13	-0.100013745737769
4	14	-0.09773358865855641
4	15	-0.11035386365082189
4	16	-0.0952161506498328
4	17	-0.10549377121650139
4	18	-0.10309248805035054
4	19	-0.121400114542908
4	20	-0.11335924394776953
4	21	-0.10464061327438401
4	22	-0.10648278489840379
4	23	-0.17701142212010978
4	24	-0.06583056215576022
4	25	-0.08811730885589952
4	26	-0.14637333914430428
4	27	-0.19553431912227381
4	28	-0.11702727198997565
4	29	-0.14632926637610666
4	30	-0.1597918002455998
4	31	-0.21061429419848565
4	32	-0.1266214825014842
4	33	-0.11945205405790205
4	34	-0.12086418908790364
4	35	-0.18823825724952314
4	36	-0.1345223080233353
4	37	-0.158119151948803
4	38	-0.16598356861179298
4	39	-0.14422117913275656
4	40	-0.17014338277297725
4	41	-0.1479552627111005
4	42	-0.15456256017977665
4	43	-0.10844802980442836
4	44	-0.11900923151849696
4	45	-0.12139805710828
4	46	-0.10431437236516672
4	47	-0.14976744353231197
4	48	-0.05965306539152948
4	49	-0.1152150416676676
4	50	-0.08951571048877582
5	1	-0.09210753758519274
5	2	-0.09213333566044923
5	3	-0.09056278507810589
5	4	-0.0905021898468732
5	5	-0.10323324658548927
5	6	-0.10009672824083538
5	7	-0.09195411591094427
5	8	-0.1000434364769518
5	9	-0.09627789912633489
5	10	-0.11131719606325419
5	11	-0.11058194061970994
5	12	-0.11928813717434394
5	13	-0.11357565213371856
5	14	-0.10417138081944444
5	15	-0.09787999251201285
5	16	-0.09579821873197858
5	17	-0.11262415458899966
5	18	-0.13536671907262843
5	19	-0.09489602083459658
5	20	-0.12804564427536036
5	21	-0.09362315516464292
5	22	-0.06896443472687963
5	23	-0.0604686364380953
5	24	-0.07848475201694782
5	25	-0.11272029379527737
5	26	-0.10129680894669946
5	27	0.0025630348963971983
5	28	-0.028949201123580792
5	29	-0.04794363317945738
5	30	-0.06914165860115418
5	31	-0.0730597263803574
5	32	-0.052130216711546495
5	33	-0.07396443335008485
5	34	-0.06035492370448026
5	35	-0.0696558448577278
5	36	-0.09830943073237819
5	37	-0.08814524209704899
5	38	-0.09353998942053465
5	39	-0.1291109435108524
5	40	-0.09155187951387234
5	41	-0.11441157733174379
5	42	-0.13954456483857314
5	43	-0.05859711204830376
5	44	-0.09389755408541589
5	45	-0.05031349999391921
5	46	-0.04561282384194243
5	47	-0.03491213660368686
5	48	-0.05893690198196566
5	49	-0.054663954399579495
5	50	-0.21616294387447965
6	1	-0.022125459182937846
6	2	-0.03283409888068434
6	3	-0.05256072816721513
6	4	0.0012234388419614106
6	5	-0.05055277400861513
6	6	-0.06790163569624497
6	7	-0.07167196791954211
6	8	-0.06203687085127996
6	9	-0.07325894595608418
6	10	-0.06504956113633398
6	11	-0.07722617935196752
6	12	-0.08032713098009481
6	13	-0.08018699179235966
6	14	-0.07728853563468618
6	15	-0.06869713020760315
6	16	-0.08503270304950661
6	17	-0.06929127196384144
6	18	-0.07332667208928001
6	19	-0.08852539669653225
6	20	-0.0871952630678905
6	21	-0.07575615497535182
6	22	-0.06631817893188696
6	23	-0.08252285171698759
6	24	-0.07731903108369664
6	25	-0.05522241640480274
6	26	-0.07842334717971511
6	27	-0.07230314514772118
6	28	-0.15271705931991164
6	29	-0.0774131624781802
6	30	-0.049510532280081065
6	31	-0.14198022827132206
6	32	-0.09353193795298864
6	33	-0.06622422241516546
6	34	-0.08250280236581631
6	35	-0.06923806994922899
6	36	-0.11035841752495656
6	37	-0.14249856267882421
6	38	-0.18155612469762872
6	39	-0.11340405861949421
6	40	-0.14010369211626994
6	41	-0.12972600194918016
6	42	-0.1532696927584638
6	43	-0.09425552983272212
6	44	-0.1108218669840042
6	45	-0.07216513695730242
6	46	-0.1326521035782946
6	47	-0.026529477445897953
6	48	0.042366773988042894
6	49	0.045211264278059105
6	50	0.011394029646268677
7	1	-0.08095982473439278
7	2	-0.14862033282322074
7	3	-0.1086574260082358
7	4	-0.3410156431650151
7	5	-0.16295734142997986
7	6	-0.13692332808327023
7	7	-0.132766268036292
7	8	-0.1298502247070985
7	9	-0.13591228292265356
7	10	-0.0927406898957912
7	11	-0.08025764335801
7	12	-0.09389049899437651
7	13	-0.10989657388285679
7	14	-0.10335680298236838
7	15	-0.1301944031669251
7	16	-0.1320761886244597
7	17	-0.14739340838846005
7	18	-0.15334876534119957
7	19	-0.13831186349831626
7	20	-0.12691741530421297
7	21	-0.11671282977879116
7	22	-0.08116828479028726
7	23	-0.08947391653226333
7	24	-0.07892990278271128
7	25	-0.07868097699204668
7	26	-0.0699178387127759
7	27	-0.0654448797737075
7	28	-0.05075379795763979
7	29	-0.03295407897731195
7	30	-0.053144338250909584
7	31	-0.044098249540518
7	32	-0.05085349362361313
7	33	-0.07134094225327203
7	34	-0.06666854845433079
7	35	-0.04955244277471138
7	36	-0.08359114836436277
7	37	-0.08943659054463637
7	38	-0.08692813841800273
7	39	-0.11694271709867365
7	40	-0.10871379777709933
7	41	-0.10873779928395777
7	42	-0.08383242663698809
7	43	-0.08174990394455506
7	44	-0.0726705791606932
7	45	-0.07830903372919806
7	46	0.00309504383828596
7	47	-0.027203904245482136
7	48	-0.07809863791085547
7	49	-0.04493158094145855
7	50	-0.09717062412047994
8	1	-0.02158778543076778
8	2	-0.0248186945062443
8	3	-0.05229271277147518
8	4	-0.025735649709289987
8	5	-0.028946554671975377
8	6	-0.041933562259245605
8	7	-0.05355484631508034
8	8	-0.05777258671003693
8	9	-0.045573368350751764
8	10	-0.04144676276306569
8	11	-0.05406580682286943
8	12	-0.049677092110464116
8	13	-0.055041763142362754
8	14	-0.047076832419456593
8	15	-0.07213402247253242
8	16	-0.05551496765495756
8	17	-0.07053451753336049
8	18	-0.06342305523617142
8	19	-0.05850361973759277
8	20	-0.029486321873001486
8	21	-0.052390593867478685
8	22	-0.04111386628586189
8	23	-0.04012747460906496
8	24	-0.07531045373982437
8	25	-0.01002389486664157
8	26	-0.008036116427959428
8	27	0.024829448439795954
8	28	0.03376167709472355
8	29	-4.6041982349009913E-4
8	30	0.05768716398483775
8	31	-0.003129151123133056
8	32	-0.053695192041125495
8	33	-0.01135181374127825
8	34	0.011742236165147801
8	35	-0.011748734704390529
8	36	-0.004467807587971606
8	37	-0.05243982555091975
8	38	-0.07386389413419892
8	39	-0.06829226349986328
8	40	-0.07253645161840439
8	41	-0.07240430502526607
8	42	-0.06269357948422538
8	43	-0.06106287941616273
8	44	-0.1277020332113139
8	45	-0.08628120488882729
8	46	-0.05668843577951321
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112	29	0.046805616430930286
112	30	0.024782694951426265
112	31	0.04792139948842333
112	32	0.01664962584138152
112	33	0.024011442972131647
112	34	0.01812630580656105
112	35	0.04595986799687779
112	36	0.0374802100476046
112	37	-0.014022148247832433
112	38	0.005881145382836905
112	39	0.0023542941833341047
112	40	0.01513574013306851
112	41	0.006229379687347603
112	42	0.01756639598041687
112	43	0.03370445326763871
112	44	0.03559233665448147
112	45	0.015956286915915996
112	46	0.1039166146937518
112	47	0.1538125921529243
112	48	0.15906067397404655
112	49	0.1331049539534277
112	50	0.18498014093523807
113	1	0.0011169711608829402
113	2	-0.0035545897095445866
113	3	0.007959371761053546
113	4	-0.04853920200863371
113	5	0.00846282039633195
113	6	-0.0022085072265483063
113	7	0.005583684486481388
113	8	-0.010994921931455792
113	9	-0.011514245088001474
113	10	-0.014724391410751991
113	11	-0.0169366098839987
113	12	-0.014543416517611263
113	13	-0.029638051755192407
113	14	-0.006085247503357039
113	15	-0.009337672701029476
113	16	-0.02230004400303187
113	17	-0.007421664829443841
113	18	-0.024472776748559966
113	19	-0.030598621262868164
113	20	-0.03463323524510997
113	21	-0.02604567367961863
113	22	-0.016127971193832025
113	23	0.011564528801059737
113	24	0.027485810965458768
113	25	-0.008450058485145462
113	26	0.0480295973435787
113	27	0.03691564211427334
113	28	0.07196046734622996
113	29	0.008349789689781062
113	30	0.04943163964891539
113	31	0.05957394368904545
113	32	0.0320027968522254
113	33	0.047913499428112516
113	34	0.025474893575079705
113	35	0.0605037620369302
113	36	0.05359228949865269
113	37	0.036016133624720226
113	38	0.05385663079788827
113	39	0.04136559341292667
113	40	0.032850969650674244
113	41	0.04124130128334258
113	42	0.05804475375007456
113	43	0.07291082032023155
113	44	0.08209307277503086
113	45	0.09994823682079357
113	46	0.15114366221382625
113	47	0.154377241146058
113	48	0.1589700879418814
113	49	0.035848110910549735
113	50	0.1398985071713028
114	1	0.022075285412263668
114	2	0.005977942573188955
114	3	0.012037351864947254
114	4	0.14091620158742302
114	5	0.018761181468597954
114	6	-0.024461875218719342
114	7	-0.020749843564267678
114	8	-0.012008610307916001
114	9	-0.008866904345410376
114	10	-0.025073464781321775
114	11	-0.04740078662575087
114	12	-0.028517367042063313
114	13	-0.04216452497360734
114	14	-0.04052683031274995
114	15	-0.04896782171617531
114	16	-0.06835573544383067
114	17	-0.047526029592667385
114	18	-0.037798880654925426
114	19	-0.0330651549262555
114	20	-0.03352328233818014
114	21	-0.04481690744626832
114	22	-0.04630386153016275
114	23	-0.036983770703244545
114	24	-0.05509450448733588
114	25	-0.05159097410744096
114	26	0.005064920976373344
114	27	0.014848660247160694
114	28	0.033499567895418636
114	29	0.06293026635842125
114	30	0.06706678625426576
114	31	0.04451746925389699
114	32	0.03292881450799712
114	33	0.03079599440211922
114	34	0.03432257356398338
114	35	0.033406446541491164
114	36	0.07766625987203213
114	37	0.01478639296422557
114	38	0.024175473615443366
114	39	0.030634180563382074
114	40	0.02266297035914988
114	41	-0.008416358885867226
114	42	0.0021013139721119956
114	43	0.026111962797941146
114	44	0.005427837527932411
114	45	0.02300365235241486
114	46	0.0424229053607732
114	47	0.09846267946715415
114	48	0.020461860162882317
114	49	0.12290682192880098
114	50	0.004826376251244824
115	1	-0.02425493968663517
115	2	-0.05474037568633605
115	3	-0.026143851633420923
115	4	0.02921227391693293
115	5	-0.039493921667393295
115	6	-0.05391398657268809
115	7	-0.05316875983329794
115	8	-0.04754964288153474
115	9	-0.04033765218394336
115	10	-0.04982890187741873
115	11	-0.06320416276382446
115	12	-0.06714508127241459
115	13	-0.06377095332909022
115	14	-0.05386378256189772
115	15	-0.048005585039334164
115	16	-0.04454945301542068
115	17	-0.05584704940527985
115	18	-0.06669903932014165
115	19	-0.04190082902443493
115	20	-0.0425088272135028
115	21	-0.040862125840369856
115	22	-0.06121776821128577
115	23	-0.03936494021169068
115	24	-0.04106067393639279
115	25	-0.04467150139562648
115	26	-0.008425356209762924
115	27	0.05108045800515981
115	28	0.011608393450252663
115	29	-0.010546500713964235
115	30	-0.0022262166814712714
115	31	0.009218698467620356
115	32	0.004480576124542779
115	33	0.008306902402829053
115	34	-0.016291870529748564
115	35	-3.422109955621977E-4
115	36	-0.007592475586385206
115	37	-0.004483815001549374
115	38	-0.008636682599359347
115	39	-0.009691476881272365
115	40	-0.04441869000529408
115	41	-0.010016079088977392
115	42	-0.058343274544753854
115	43	0.007500573697370783
115	44	0.03213325657542043
115	45	-0.004833323988876259
115	46	0.04227817388890287
115	47	0.05494747660385002
115	48	-0.12877178291167724
115	49	0.04765027569949609
115	50	0.014798173843736606
116	1	-0.009078720351006098
116	2	-0.022219193991880104
116	3	-0.021734356379901953
116	4	0.008507452774935587
116	5	-0.00534948173092431
116	6	-0.033311469654606185
116	7	-0.03748000005499108
116	8	-0.04548938889395515
116	9	-0.02963277162734812
116	10	-0.03492204096340146
116	11	-0.03205497617033615
116	12	-0.04862898096782686
116	13	-0.06975571599168973
116	14	-0.06270522113459265
116	15	-0.055411743240277644
116	16	-0.06871714885404145
116	17	-0.06730194054308924
116	18	-0.08270267810154053
116	19	-0.0669465339564681
116	20	-0.07007032713071482
116	21	-0.06160844537003385
116	22	-0.05289314021852931
116	23	-0.045740948461116204
116	24	0.017498496166382438
116	25	-0.05311990827032531
116	26	-0.02953849430452493
116	27	-0.021573994397627416
116	28	-0.04724428660313862
116	29	-0.02760673215705367
116	30	-0.024358172793604638
116	31	0.003355850519497494
116	32	-0.005286104769481881
116	33	-1.499161831048923E-4
116	34	0.01442087589516916
116	35	0.023937082354947847
116	36	-6.240753275577049E-4
116	37	-0.033572068058731475
116	38	-0.01162563634198932
116	39	-0.03675557008956787
116	40	-0.023736225098041075
116	41	-0.018257623067022166
116	42	-0.04132089121071658
116	43	0.009721619014662508
116	44	0.04989138915054525
116	45	0.033275598617194646
116	46	0.10050812100063666
116	47	0.12128750715617542
116	48	0.216544703453728
116	49	0.10117934808416607
116	50	0.15314252610556167
117	1	-0.008746240802366856
117	2	-0.01901773200428636
117	3	-0.01263179613815879
117	4	0.02651346535083121
117	5	-0.05742606136147543
117	6	-0.044108115612857546
117	7	-0.04142208702817385
117	8	-0.04172402748421433
117	9	-0.057499685395093536
117	10	-0.06902877246019301
117	11	-0.07309000630239382
117	12	-0.08104075858445015
117	13	-0.06311789948838964
117	14	-0.06083207666483759
117	15	-0.05806707603046135
117	16	-0.06616439502537474
117	17	-0.05724459019483419
117	18	-0.050170252867388854
117	19	-0.03487268904142127
117	20	-0.034465389341420405
117	21	-0.06432115835160346
117	22	-0.07102831926778919
117	23	-0.10117987165603637
117	24	-0.12135361785280452
117	25	-0.0636886884000134
117	26	0.00479366131055059
117	27	0.031007368136393154
117	28	-0.02853283679968399
117	29	-6.413249516725728E-4
117	30	-0.010711291305270265
117	31	-0.014015561556817602
117	32	0.0010123140060755986
117	33	-0.053037225014143985
117	34	-0.02736180592204107
117	35	-0.03503355770684635
117	36	-0.033474058547582786
117	37	-0.08462462153151051
117	38	-0.043370474363001676
117	39	-0.05375532673244976
117	40	-0.03552843772433789
117	41	-0.17183300585258365
117	42	-0.2948213406210556
117	43	-0.22693471921755304
117	44	-0.10672473682651429
117	45	-0.07689512847800017
117	46	-0.058200876240505295
117	47	-0.22832763860986205
117	48	-0.39145330845150994
117	49	-0.01587716475746248
117	50	-0.019286034199666346
118	1	0.009045130074355257
118	2	-0.01325531414883585
118	3	-0.023235560990691795
118	4	0.012364984337565943
118	5	0.009659087103095487
118	6	-0.028833751496378568
118	7	-0.07301378040130402
118	8	-0.05968752568151814
118	9	-0.05094067174303518
118	10	-0.06095364571728368
118	11	-0.051704004363777756
118	12	-0.06318395542098898
118	13	-0.048101084131033645
118	14	-0.05845448554167376
118	15	-0.058341054962561145
118	16	-0.050497169596056324
118	17	-0.05956599583507938
118	18	-0.0298032843892031
118	19	-0.052913007068440265
118	20	-0.05312321249573415
118	21	-0.04636935988840207
118	22	-0.027616044097058534
118	23	-0.019049959295216468
118	24	0.001223547840076833
118	25	-0.006664237918087679
118	26	0.0037113868477547385
118	27	0.045377379972599385
118	28	0.017523087691451167
118	29	0.0020621847871851173
118	30	6.702070533677329E-4
118	31	0.0196259163107797
118	32	0.003963105875889994
118	33	-0.01580416663794182
118	34	-0.01709440470830259
118	35	-0.0032079945281751066
118	36	-0.011346968394548185
118	37	-0.030570702318307497
118	38	-0.02317242804316777
118	39	-0.0359974257176674
118	40	-0.03359078038930363
118	41	-0.01798865608233058
118	42	-0.00827766417570075
118	43	0.02363594088798493
118	44	0.04184230441896375
118	45	0.0247976491111217
118	46	0.10587194302345893
118	47	0.0829479764118588
118	48	0.13849088693874734
118	49	0.09634985718032851
118	50	0.1352975871299833
119	1	-0.012235203406966377
119	2	-0.047410720284972285
119	3	-0.039925176300464216
119	4	-0.0279629070936096
119	5	-0.06340297553281005
119	6	-0.03830992025508806
119	7	-0.04459232667849733
119	8	-0.06179336109978095
119	9	-0.055234087884720395
119	10	-0.054605096534494635
119	11	-0.06814696886242899
119	12	-0.06609299554052939
119	13	-0.07709612457382775
119	14	-0.07629454989183415
119	15	-0.08516929669296047
119	16	-0.08476396368635619
119	17	-0.09466626725504312
119	18	-0.08968205805737739
119	19	-0.11131727089045995
119	20	-0.1205634795104018
119	21	-0.13740378400486009
119	22	-0.13055496044032822
119	23	-0.12547996700368458
119	24	-0.09639314488391193
119	25	-0.06260625585591839
119	26	-0.06559304906562602
119	27	-0.07985242254441971
119	28	-0.014354910847757196
119	29	-0.04824124733664803
119	30	-0.14728737306471373
119	31	-0.0422879494537014
119	32	-0.07322525296706317
119	33	-0.06910938470799266
119	34	-0.062203618882819
119	35	-0.049536891160869345
119	36	-0.08529081652331882
119	37	-0.08727597315636615
119	38	-0.10391845873972727
119	39	-0.10712484946501633
119	40	-0.08084419441050983
119	41	-0.0858211649188263
119	42	-0.08317212340946156
119	43	-0.03372733390629179
119	44	-0.03040781127288028
119	45	-0.025798909054003616
119	46	0.04837727587194962
119	47	0.023683806821480857
119	48	0.15317096889780402
119	49	0.049016239914848825
119	50	0.0697094360907684
120	1	-0.0426082251390838
120	2	-0.09760258066985017
120	3	-0.07612018169251655
120	4	-0.06169200141317788
120	5	-0.08868804147731879
120	6	-0.10337296767263382
120	7	-0.11388832728174236
120	8	-0.11711047146400944
120	9	-0.11608306813977265
120	10	-0.10672700345096331
120	11	-0.13643507488411188
120	12	-0.19035701858067
120	13	-0.1458136842127402
120	14	-0.15341863276919554
120	15	-0.1389307661421455
120	16	-0.11186464502880966
120	17	-0.10934655348673772
120	18	-0.11531885950257958
120	19	-0.10190996923100926
120	20	-0.11433102727139755
120	21	-0.10285997324827179
120	22	-0.09309965847408819
120	23	-0.05322768169370562
120	24	-0.04885309396561155
120	25	-0.07066059587395301
120	26	-0.02772275827313564
120	27	-7.756531545766165E-4
120	28	-0.05330697858575917
120	29	-0.06778695543470548
120	30	-0.07644880582326863
120	31	-0.04514137916456207
120	32	-0.06172076325522369
120	33	-0.03567337460567899
120	34	-0.03911548777850271
120	35	-0.06000199093077896
120	36	-0.04970188156837452
120	37	-0.03394315659490843
120	38	-0.01141779505981333
120	39	-0.012500311054360225
120	40	-0.038569155209422945
120	41	-0.04435670074733622
120	42	-0.032901989563391965
120	43	0.024146695173676846
120	44	0.02975511599993297
120	45	0.050077693226171505
120	46	0.14658323625955205
120	47	0.2021668172055442
120	48	0.16094766241895542
120	49	0.23291839383502122
120	50	0.13275347420718475
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	4908.0
3	351.0
4	220.0
5	312.0
6	443.0
7	802.0
8	975.0
9	1410.0
10	1997.0
11	3100.0
12	3633.0
13	5018.0
14	7179.0
15	8542.0
16	9280.0
17	11021.0
18	13049.0
19	15450.0
20	17431.0
21	17908.0
22	22812.0
23	29091.0
24	32744.0
25	39181.0
26	53215.0
27	69477.0
28	86729.0
29	106672.0
30	130969.0
31	165035.0
32	208380.0
33	274408.0
34	384982.0
35	579197.0
36	944192.0
37	1759558.0
38	3509178.0
39	1.8333864E7
40	564688.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	17.858413894241117	29.597451754728	25.147985853174553	27.39614849785633
2	23.65651469042034	29.779800621425405	26.159690697355238	20.40399399079902
3	23.35068961596831	27.967189910812223	25.94483458886226	22.73728588435721
4	22.94790340107728	26.994168595593283	26.658032197840843	23.399895805488594
5	23.70835951956205	28.13236746984151	27.323279110226384	20.835993900370063
6	23.899734332951546	27.03134042500965	25.34264279827253	23.726282443766276
7	23.22056711356412	27.60158776537572	27.06962632964372	22.108218791416444
8	23.322146341378016	27.096448541599905	26.936751781616064	22.64465333540602
9	22.60492566034376	26.493536155428288	27.38981304325165	23.5117251409763
10	22.42981120036555	27.975174718599042	26.58809069709195	23.00692338394346
11	23.180482962654533	27.561897101828702	26.45206004167141	22.805559893845352
12	22.874110772867397	27.070729588800084	27.053200181815622	23.001959456516893
13	22.56869654123193	27.723227650536565	26.585117942654797	23.122957865576712
14	22.62940328081808	28.146282015608428	26.753628329545403	22.470686374028084
15	22.28753092471058	27.430288500293514	27.359026615987336	22.92315395900857
16	23.011717872504743	27.098969342367855	26.886530860441688	23.002781924685717
17	22.992384968556806	26.999957435511064	26.85518346297752	23.152474132954612
18	22.35108932494056	27.100785986472165	26.760177380832673	23.787947307754607
19	23.298960732177584	27.363378785563835	26.71783706649561	22.61982341576297
20	23.01520529495239	27.40920651104335	26.896020648525678	22.679567545478584
21	23.012407231713656	27.41172013108834	26.75563284351493	22.82023979368307
22	23.00351517636555	27.370479061819168	26.730097429730847	22.89590833208443
23	22.604677226699934	26.94517616750034	27.83030747516878	22.619839130630943
24	22.51655435903644	27.208501637336084	26.832933581122443	23.442010422505035
25	23.177419333072454	26.909556452852694	26.67220353964258	23.240820674432268
26	23.223039995658233	26.874250407615225	27.314350473992775	22.58835912273377
27	22.826733009110292	27.20070218336279	27.16200404561493	22.810560761911987
28	22.793309256409824	27.324741685034258	27.003967297994436	22.87798176056148
29	23.295534952256595	26.88419397900603	27.108256070962234	22.712014997775135
30	22.804259696850494	27.228481529647926	27.09412450638767	22.873134267113915
31	23.28031574736271	26.902503137211898	27.00736871774184	22.80981239768355
32	22.82746870679056	26.84127383701628	27.06238211462198	23.26887534157117
33	23.20944493331905	26.839722804634143	27.013569069992705	22.937263192054104
34	23.318170074141257	26.80438461822704	27.063428341079476	22.814016966552224
35	22.78095439858786	26.885267882822504	27.552754015890557	22.781023702699077
36	22.80662155726645	26.739779767768685	27.54915262299062	22.904446051974247
37	22.910112205864248	26.803262678441975	26.911779108097882	23.374846007595895
38	22.73996584966485	27.552913359737456	27.01928792896462	22.687832861633076
39	23.23363931749964	26.83911762550577	26.994403443951438	22.93283961304315
40	22.851004694287706	26.802901923308397	26.922845168749976	23.42324821365392
41	22.77638841998773	27.495460372105047	27.019255375552625	22.708895832354596
42	23.404187101746427	26.69406943427835	27.00255939354552	22.8991840704297
43	23.29453956337896	26.79241303809741	26.942937806658467	22.970109591865157
44	22.87995394761775	27.364852529771493	27.051215146979267	22.703978375631483
45	22.817739739705058	27.22854727773578	27.011995305827867	22.941717676731297
46	22.800096916553102	26.797481643136052	27.53115074619947	22.871270694111377
47	23.3736961428255	26.81037856214015	27.08723558443778	22.728689710596566
48	22.730191581552095	26.65809010481608	26.972587949575594	23.63913036405623
49	22.818037513516522	26.875815336196894	26.85586812827532	23.450279022011262
50	23.489656471870724	26.80189861222238	26.939042003751716	22.769402912155172
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	7656.0
1	4410.5
2	1165.0
3	963.5
4	762.0
5	825.5
6	889.0
7	1115.0
8	1341.0
9	2194.5
10	3048.0
11	5264.5
12	7481.0
13	12457.0
14	17433.0
15	27376.0
16	37319.0
17	57324.0
18	77329.0
19	107153.0
20	136977.0
21	180229.0
22	223481.0
23	325226.0
24	426971.0
25	443257.5
26	459544.0
27	535673.0
28	611802.0
29	693620.5
30	775439.0
31	861843.0
32	948247.0
33	1033578.5
34	1118910.0
35	1218959.0
36	1319008.0
37	1402062.5
38	1485117.0
39	1561478.0
40	1637839.0
41	1698311.5
42	1758784.0
43	1776039.5
44	1793295.0
45	1794120.5
46	1794946.0
47	1776841.5
48	1758737.0
49	1726143.5
50	1693550.0
51	1663821.5
52	1634093.0
53	1573549.5
54	1513006.0
55	1430523.0
56	1348040.0
57	1283486.0
58	1218932.0
59	1195084.5
60	1171237.0
61	996191.5
62	821146.0
63	702668.5
64	584191.0
65	491603.0
66	399015.0
67	329095.0
68	259175.0
69	212609.0
70	166043.0
71	134287.5
72	102532.0
73	77955.0
74	53378.0
75	39274.5
76	25171.0
77	18955.0
78	12739.0
79	9327.5
80	5916.0
81	4360.0
82	2804.0
83	2062.0
84	1320.0
85	983.5
86	647.0
87	481.5
88	316.0
89	231.0
90	146.0
91	111.0
92	76.0
93	56.5
94	37.0
95	34.0
96	31.0
97	46.5
98	62.0
99	170.0
100	278.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.09827700298799293
2	4.0849969696252393E-4
3	0.004376782467455613
4	0.040000144433821425
5	0.0
6	0.0
7	0.0
8	0.0016011729193441786
9	7.440530194674543E-4
10	1.0212492424063098E-4
11	1.5683470508382613E-4
12	0.002243101014571002
13	3.28258685059171E-5
14	1.1671419913214969E-4
15	0.005919598287233718
16	1.8966057358974324E-4
17	6.56517370118342E-4
18	2.9178549783037422E-5
19	4.303836092998019E-4
20	1.604820238067058E-4
21	6.018075892751468E-4
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	2.2248644209566036E-4
28	0.0
29	3.6473187228796778E-6
30	0.0032679975757001914
31	0.02231794326530075
32	0.026629073995744528
33	0.014954006763806678
34	0.011343161228155797
35	0.007294637445759355
36	0.0072107491151331225
37	0.0046539786903944695
38	0.0035707250296992043
39	0.007834440616745549
40	0.004015697913890525
41	0.003855215890083819
42	0.007010146585374741
43	0.004796224120586777
44	0.006069138354871784
45	0.0015537577759467429
46	0.0
47	0.0
48	1.5683470508382613E-4
49	1.0941956168639033E-4
50	4.0849969696252393E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2.7417401E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.16976116656075
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.43127210790098	31.26840717888258
2	13.924580761151134	12.022416516067622
3	4.790331220654047	6.203923641130638
4	2.3100658716301297	3.988999678291827
5	1.3648399307195587	2.9459906919874332
6	0.8874321923552503	2.2986141477296123
7	0.6419708131371811	1.9399608675324433
8	0.47553677386935916	1.642304716308563
9	0.3668301522040844	1.4252373053409102
>10	2.4849152381760913	21.146670317826498
>50	0.2288646729997648	6.704980400676849
>100	0.0923772667858076	7.122095035827221
>500	8.981468939633601E-4	0.2329933420414104
>1k	1.7108679521082892E-5	0.008777895200097521
>5k	4.2339277039356105E-5	0.11828272697981758
>10k+	2.5403566220717988E-5	0.9303455381765744
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCG	140525	0.5125394635326668	Illumina Paired End PCR Primer 2 (100% over 31bp)
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	83715	0.30533528688587225	No Hit
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAG	30262	0.11037515919178481	Illumina Paired End PCR Primer 2 (97% over 36bp)
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	6.929905573471388E-5	3.6473187228796778E-6	0.0	0.0	0.0
2	8.024101190335292E-5	3.6473187228796778E-6	0.0	3.6473187228796778E-6	0.0
3	1.0212492424063098E-4	3.6473187228796778E-6	0.0	3.6473187228796778E-6	0.0
4	1.1306688040927001E-4	3.6473187228796778E-6	0.0	3.6473187228796778E-6	0.0
5	1.313034740236684E-4	3.6473187228796778E-6	0.0	3.6473187228796778E-6	0.0
6	1.3859811146942777E-4	3.6473187228796778E-6	0.0	3.6473187228796778E-6	0.0
7	1.495400676380668E-4	7.2946374457593555E-6	0.0	3.6473187228796778E-6	0.0
8	1.823659361439839E-4	7.2946374457593555E-6	0.0	1.0941956168639033E-5	0.0
9	2.2613376081854003E-4	7.2946374457593555E-6	0.0	1.0941956168639033E-5	0.0
10	7.221691071301762E-4	7.2946374457593555E-6	0.0	1.0941956168639033E-5	0.0
11	7.987628003106494E-4	7.2946374457593555E-6	0.0	1.4589274891518711E-5	0.0
12	0.0010030126487919114	1.0941956168639033E-5	0.0	1.4589274891518711E-5	3.6473187228796778E-6
13	0.0011087848917554221	1.0941956168639033E-5	0.0	1.4589274891518711E-5	3.6473187228796778E-6
14	0.0011853785849358952	1.0941956168639033E-5	0.0	1.4589274891518711E-5	3.6473187228796778E-6
15	0.005941482199570995	1.0941956168639033E-5	0.0	1.4589274891518711E-5	3.6473187228796778E-6
16	0.006236915016124249	1.0941956168639033E-5	0.0	1.4589274891518711E-5	3.6473187228796778E-6
17	0.007006499266651861	1.0941956168639033E-5	0.0	1.4589274891518711E-5	3.6473187228796778E-6
18	0.008078810971178487	1.0941956168639033E-5	0.0	1.4589274891518711E-5	3.6473187228796778E-6
19	0.010493335965724833	1.0941956168639033E-5	0.0	1.4589274891518711E-5	3.6473187228796778E-6
20	0.5493263201716312	1.0941956168639033E-5	0.0	1.4589274891518711E-5	3.6473187228796778E-6
21	0.557244649119003	1.0941956168639033E-5	3.6473187228796778E-6	1.4589274891518711E-5	3.6473187228796778E-6
22	0.5594184510778392	1.0941956168639033E-5	3.6473187228796778E-6	1.4589274891518711E-5	3.6473187228796778E-6
23	0.5606475974874496	1.0941956168639033E-5	3.6473187228796778E-6	1.4589274891518711E-5	3.6473187228796778E-6
24	0.5613004675388451	1.0941956168639033E-5	3.6473187228796778E-6	1.823659361439839E-5	3.6473187228796778E-6
25	0.5616870833234704	1.0941956168639033E-5	3.6473187228796778E-6	2.1883912337278066E-5	3.6473187228796778E-6
26	0.562365484605926	1.823659361439839E-5	1.0941956168639033E-5	2.1883912337278066E-5	7.2946374457593555E-6
27	0.5629052877769122	2.5531231060157746E-5	1.0941956168639033E-5	2.1883912337278066E-5	1.0941956168639033E-5
28	0.5851502846677553	2.9178549783037422E-5	1.0941956168639033E-5	2.1883912337278066E-5	1.0941956168639033E-5
29	0.5866748638939191	2.9178549783037422E-5	1.0941956168639033E-5	2.1883912337278066E-5	1.0941956168639033E-5
30	0.5898079106768727	3.28258685059171E-5	1.0941956168639033E-5	2.1883912337278066E-5	1.0941956168639033E-5
31	0.7058874763512413	3.647318722879678E-5	1.0941956168639033E-5	2.1883912337278066E-5	1.0941956168639033E-5
32	0.70753241709526	3.647318722879678E-5	1.0941956168639033E-5	2.1883912337278066E-5	1.0941956168639033E-5
33	0.7080612783100776	3.647318722879678E-5	1.0941956168639033E-5	2.1883912337278066E-5	1.0941956168639033E-5
34	0.7083749477202452	3.647318722879678E-5	1.0941956168639033E-5	2.1883912337278066E-5	1.0941956168639033E-5
35	0.7087652108235933	3.647318722879678E-5	1.0941956168639033E-5	2.1883912337278066E-5	1.0941956168639033E-5
36	0.7091554739269416	3.647318722879678E-5	1.0941956168639033E-5	2.1883912337278066E-5	1.0941956168639033E-5
37	0.7095457370302897	3.647318722879678E-5	1.0941956168639033E-5	2.1883912337278066E-5	1.0941956168639033E-5
38	0.7101220133885047	3.647318722879678E-5	1.0941956168639033E-5	2.1883912337278066E-5	1.0941956168639033E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGATCGG	21645	0.0	29.917898	20
ATGCCGA	21910	0.0	29.497906	14
TGCCGAG	22085	0.0	29.314026	15
CGAGATC	23385	0.0	27.654337	18
CCGAGAT	23765	0.0	27.203037	17
GAGATCG	24080	0.0	26.892664	19
GCCGAGA	24295	0.0	26.700235	16
GATCGGA	24360	0.0	26.41184	21
ATCGGAA	25230	0.0	25.431295	22
AGAGCGG	25330	0.0	25.22486	28
TCGGAAG	25910	0.0	24.823286	23
GAGCGGT	25740	0.0	24.686434	29
AAGAGCG	26135	0.0	24.414131	27
GCGGTTC	26130	0.0	24.36008	31
AGCGGTT	26660	0.0	23.900562	30
AAGCAGT	25120	0.0	22.46549	1
GAGTACT	20475	0.0	22.452944	20
CAACGCA	24220	0.0	22.016052	13
TCAACGC	24725	0.0	21.638033	12
ATCAACG	24855	0.0	21.515968	11
>>END_MODULE