FastQCFastQC Report
Thu 26 May 2016
SRR522124_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR522124_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27703451
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCG1099160.396759234075206Illumina Paired End PCR Primer 2 (100% over 31bp)
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT346880.12521183732669264No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA154850.033.15506421
AGATCGG159700.032.285920
ATGCCGA161900.032.0664214
ATCGGAA167100.030.75081622
TGCCGAG172650.030.17174515
CGAGATC173600.029.81505218
AAGAGCG172500.029.56611827
AGAGCGG174600.029.38693628
GAGATCG176200.029.37489319
CCGAGAT177050.029.32088917
TCGGAAG176100.029.27915223
GAGCGGT176850.028.71446829
GCCGAGA182700.028.5346416
AGCGGTT181500.027.97880730
GCGGTTC182450.027.96577631
CGGAAGA212700.024.35527824
AATGCCG274450.018.90819413
GAAGAGC284250.018.49981926
CGGTTCA287650.018.1278381
GAATGCC341200.015.46754612
Produced by FastQC (version 0.11.5)